##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099255_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	78185
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.353763509624606	37.0	35.0	37.0	33.0	37.0
2	35.2170237257786	37.0	35.0	37.0	32.0	37.0
3	35.70215514484875	37.0	36.0	37.0	33.0	37.0
4	37.08298266930997	39.0	38.0	39.0	33.0	39.0
5	36.56315149964827	37.0	35.0	39.0	33.0	39.0
6	36.70003197544286	39.0	37.0	39.0	32.0	39.0
7	35.8706529385432	39.0	37.0	39.0	30.0	39.0
8	36.88699878493317	39.0	37.0	39.0	32.0	39.0
9	31.98864232269617	33.0	31.0	34.0	30.0	34.0
10	32.18054614056405	34.0	31.0	34.0	30.0	34.0
11	32.2211549529961	34.0	31.0	34.0	30.0	34.0
12	35.50848628253501	37.0	35.0	37.0	33.0	37.0
13	35.60181620515444	37.0	35.0	37.0	33.0	37.0
14	31.669220438703075	31.0	31.0	34.0	30.0	34.0
15	31.721506682867556	31.0	31.0	34.0	30.0	34.0
16	31.332212061137046	31.0	31.0	34.0	28.0	34.0
17	34.94458016243525	35.0	35.0	37.0	32.0	37.0
18	34.910366438575174	35.0	35.0	37.0	32.0	37.0
19	33.93202020847989	35.0	33.0	37.0	28.0	37.0
20	33.33512822152587	35.0	32.0	37.0	26.0	37.0
21	33.165236298522736	35.0	32.0	37.0	25.0	37.0
22	34.40414401739464	37.0	32.0	39.0	25.0	39.0
23	34.593540960542306	37.0	32.0	39.0	26.0	39.0
24	34.933337596725714	37.0	33.0	39.0	27.0	39.0
25	35.1796891986954	37.0	34.0	39.0	27.0	39.0
26	35.296067020528234	37.0	34.0	39.0	27.0	39.0
27	36.65923131035365	39.0	34.0	41.0	30.0	41.0
28	36.96705250367717	39.0	35.0	41.0	30.0	41.0
29	37.25779881051353	39.0	36.0	41.0	31.0	41.0
30	37.49451940909382	39.0	36.0	41.0	32.0	41.0
31	37.741727952932145	39.0	36.0	41.0	32.0	41.0
32	38.02198631451046	40.0	37.0	41.0	33.0	41.0
33	38.20556372705762	40.0	37.0	41.0	34.0	41.0
34	38.32329730766771	40.0	37.0	41.0	34.0	41.0
35	38.50273070282024	40.0	37.0	41.0	35.0	41.0
36	38.540781479823494	40.0	37.0	41.0	35.0	41.0
37	38.55872609835646	40.0	37.0	41.0	35.0	41.0
38	38.449945641747135	40.0	37.0	41.0	35.0	41.0
39	38.466304278314254	40.0	37.0	41.0	35.0	41.0
40	38.44466329858668	40.0	38.0	41.0	35.0	41.0
41	38.3365351410117	40.0	38.0	41.0	35.0	41.0
42	38.18590522478736	40.0	38.0	41.0	35.0	41.0
43	38.04527722708959	40.0	38.0	41.0	35.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.3268724160412617
1101	2	-0.30696971783141436
1101	3	-0.09876294691116527
1101	4	-0.339734190487853
1101	5	0.44673281173522383
1101	6	-0.09570928040211157
1101	7	-0.06942840180481369
1101	8	-0.052714165150831604
1101	9	0.4545576339649706
1101	10	0.04960664912354673
1101	11	0.21507812019864758
1101	12	0.04145202614125054
1101	13	0.2576225932928011
1101	14	0.019645211249208927
1101	15	-0.12300153228007815
1101	16	-0.15932011452727934
1101	17	0.04386269115991581
1101	18	-0.21388361122426147
1101	19	-0.5912878434222293
1101	20	-0.2118224468593155
1101	21	-0.08610687648989312
1101	22	-0.4689112729779126
1101	23	-0.572409885074137
1101	24	-1.063247768653497
1101	25	-0.8943428208030397
1101	26	-1.1750201778632743
1101	27	-1.832306314843784
1101	28	-1.292718977967148
1101	29	-1.1252980120379803
1101	30	-0.7811548997182385
1101	31	-0.37163206953248107
1101	32	-0.6667044617706921
1101	33	-0.6762325597710799
1101	34	-0.5017201168301781
1101	35	-0.8108095751904827
1101	36	-0.6649613511723302
1101	37	-0.19434068258848924
1101	38	-0.1497848718694499
1101	39	-0.215943868033591
1101	40	-0.5305703927048739
1101	41	-0.4357239900272418
1101	42	-0.45192446831254784
1101	43	0.3020008822050926
1102	1	0.3145153840279278
1102	2	0.7544705948302379
1102	3	0.5701835442664915
1102	4	0.7320515443244915
1102	5	0.330519236242381
1102	6	0.8354361877282415
1102	7	0.8938453495602019
1102	8	0.2845465969619312
1102	9	0.2287400582422272
1102	10	0.21599552204727956
1102	11	0.3390507623045167
1102	12	0.4230256797995082
1102	13	0.6869001379792792
1102	14	0.08085562564084015
1102	15	-0.20990307834594546
1102	16	-0.026553863593317573
1102	17	-0.027405772544895513
1102	18	-0.5648807949704562
1102	19	-0.6492222074661456
1102	20	-1.196994008443312
1102	21	-0.7122922894446617
1102	22	-1.206137550350178
1102	23	-0.4984401166966492
1102	24	-0.3696446880166775
1102	25	-0.8118668164349714
1102	26	-0.9152926073132477
1102	27	-1.766268036781824
1102	28	-1.877695126021635
1102	29	-0.8936180304298418
1102	30	-1.0293875567674888
1102	31	-1.2459351904014966
1102	32	-0.8677504988992624
1102	33	-0.5288102941236232
1102	34	-0.5103987765232603
1102	35	-0.5486680728052917
1102	36	-0.24700629628411974
1102	37	0.07349080197958813
1102	38	-0.04581337925493045
1102	39	-0.23376098395979739
1102	40	0.13268611628989646
1102	41	-0.20812470271187777
1102	42	0.3447879864262262
1102	43	0.35372799295858925
1103	1	0.7059681742327797
1103	2	0.8025045011170278
1103	3	0.5835459177065658
1103	4	0.5082465054735366
1103	5	0.7046068274832535
1103	6	1.127112665241782
1103	7	0.7564542499604912
1103	8	0.7645324693424342
1103	9	0.5285987820472648
1103	10	0.522682595275441
1103	11	0.6038258976942004
1103	12	0.7867530167432335
1103	13	0.680660439368495
1103	14	-0.10144953094027542
1103	15	-0.036133358543732896
1103	16	-0.033970863828781006
1103	17	0.01909764162981986
1103	18	-0.2289966414503226
1103	19	0.248882336918129
1103	20	-0.6022212276568766
1103	21	-0.5438792920347311
1103	22	-0.2753628858811368
1103	23	0.0022662642781625664
1103	24	-0.3237887897355378
1103	25	-0.37732478676696957
1103	26	-0.08288149358590857
1103	27	-0.9824206150723782
1103	28	-0.2697954321200555
1103	29	-0.34805645755486836
1103	30	-0.21581326903497455
1103	31	0.01654420682007185
1103	32	-0.08555129946436324
1103	33	0.020871834437713233
1103	34	0.06647901956809932
1103	35	0.2385935236157124
1103	36	0.20295838466714144
1103	37	0.33785388180063336
1103	38	0.6633342543687988
1103	39	0.5399616437774313
1103	40	0.8060437805234741
1103	41	0.7549668913545204
1103	42	0.8388426132216154
1103	43	0.8251784285601858
1104	1	0.2766286965062079
1104	2	0.0014292322998201712
1104	3	0.16739128380999801
1104	4	0.07865101136191299
1104	5	0.0895274624038862
1104	6	-0.027905255905167792
1104	7	-0.007498643024661078
1104	8	0.04077414880992336
1104	9	0.3545076402141838
1104	10	0.2973881764326336
1104	11	0.13890014244586268
1104	12	0.09282060506888712
1104	13	0.3434868602594321
1104	14	0.24711677730345372
1104	15	0.27851077868104923
1104	16	0.3817997454902162
1104	17	0.2024053733246447
1104	18	-0.08921169521376271
1104	19	0.07850343266825632
1104	20	0.4881525562652982
1104	21	-0.6092914784146544
1104	22	-0.3022446063112483
1104	23	-0.008230407508833082
1104	24	0.29308269003916365
1104	25	0.18795939142042783
1104	26	0.2308665862912065
1104	27	-0.0720263498394047
1104	28	0.4105681111467092
1104	29	0.6058605931363701
1104	30	0.7164703971196573
1104	31	0.6869231110699445
1104	32	0.3916632422663042
1104	33	-0.0057538696073464735
1104	34	0.15557272156502222
1104	35	0.32973535669302834
1104	36	0.11975305952633164
1104	37	0.1733633544068809
1104	38	0.38414838647325666
1104	39	-0.007657403841619725
1104	40	0.38937711385680984
1104	41	0.31730688111386485
1104	42	0.5693853679579206
1104	43	0.4154242042898346
1105	1	0.32958107745858456
1105	2	0.5947625656331539
1105	3	0.581803982222695
1105	4	0.7437303764412775
1105	5	0.1506068274832515
1105	6	0.9529836329837167
1105	7	1.0554219918959689
1105	8	0.8477582757940496
1105	9	0.3968568465633915
1105	10	0.573134208178665
1105	11	0.41350331704904164
1105	12	0.625011081259359
1105	13	0.47872495549752614
1105	14	-0.16977211158543426
1105	15	-0.33755271338243986
1105	16	0.04357752326799513
1105	17	-0.07651526159598632
1105	18	-0.3945450285470997
1105	19	-0.8009886308238094
1105	20	-1.424672840560099
1105	21	-1.2448470339702098
1105	22	-1.1889112729779114
1105	23	-0.9950240583024836
1105	24	-1.5688855639290864
1105	25	-1.0804215609605237
1105	26	-1.2287524613278435
1105	27	-0.3795819053949643
1105	28	-0.6988922063136087
1105	29	-0.48328226400648333
1105	30	-0.6419423012930352
1105	31	-0.5095848254379902
1105	32	-0.4770351704321101
1105	33	-0.3485475204009987
1105	34	-0.46461775462545063
1105	35	-0.5354709925133179
1105	36	-0.29897709920382454
1105	37	0.026950655994184558
1105	38	0.20662457694944436
1105	39	0.4279616437774294
1105	40	-0.28395621947652927
1105	41	-0.509613753806768
1105	42	-1.4169638383912897
1105	43	-1.2133377004720671
1106	1	-0.048768128890621654
1106	2	-0.3239675930970023
1106	3	-0.06276744634873666
1106	4	0.1738891066000079
1106	5	0.5974639703403994
1106	6	-0.15488938288930143
1106	7	-0.11860975413577535
1106	8	-0.14970204166626644
1106	9	0.13228541799196236
1106	10	0.11484849389294993
1106	11	0.27382077736650245
1106	12	-0.13733812508984755
1106	13	0.21650273327530556
1106	14	0.09632312650980168
1106	15	0.07216157233184362
1106	16	0.1516410153314851
1106	17	-0.011880340961070601
1106	18	-0.6288942348963076
1106	19	-0.8262584720936488
1106	20	-0.35311728500454365
1106	21	-0.3553232244464013
1106	22	-0.4451017491683871
1106	23	-0.9288653281437576
1106	24	-0.691044294087817
1106	25	-0.6850564815954385
1106	26	-0.25326039783578125
1106	27	-0.5244073022203608
1106	28	0.07723477781337351
1106	29	-0.4655679782921993
1106	30	-0.22797404732478554
1106	31	-0.487680063533233
1106	32	-0.2988129482098856
1106	33	-0.40257926643274544
1106	34	-0.1380780720857686
1106	35	0.23449726145493344
1106	36	0.10388004365331938
1106	37	0.20510938615291252
1106	38	0.36033886266373116
1106	39	0.20662831044409558
1106	40	-0.2137974893177983
1106	41	-0.09539153158454639
1106	42	-0.009979711407162029
1106	43	-0.31473452586889294
1107	1	0.05074347062097928
1107	2	0.20228393315452564
1107	3	0.20341081982952858
1107	4	0.16595259866350176
1107	5	0.03580340867983267
1107	6	0.061838333838416304
1107	7	0.19579806027203972
1107	8	-0.20831010027432484
1107	9	-0.10153631582976885
1107	10	-0.10248972344526663
1107	11	-0.06683856329284055
1107	12	-0.20876669651841695
1107	13	-0.5185400017674269
1107	14	-0.11185758167090398
1107	15	0.05567805584832897
1107	16	0.1296629933534632
1107	17	0.23842490934418237
1107	18	-0.016562122564195647
1107	19	0.468002822167648
1107	20	-0.015510447397709015
1107	21	0.057375188252009934
1107	22	-0.45181725588389554
1107	23	-0.06928046855889392
1107	24	0.22714862410509795
1107	25	-0.16185745839641896
1107	26	-0.9748721194475038
1107	27	-0.6617699395829959
1107	28	-0.35133665075805
1107	29	-0.046154058878279614
1107	30	-0.2768140961648342
1107	31	0.1734920976389276
1107	32	-0.01248816188509494
1107	33	0.3990080351545586
1107	34	0.07254463853693949
1107	35	0.28394781090548094
1107	36	0.12768956746283777
1107	37	0.4664036474471729
1107	38	0.5062485085733783
1107	39	-0.049781945966159924
1107	40	-0.3072040827243896
1107	41	-0.33470777090078485
1107	42	-1.0343997358271864
1107	43	-0.74147445260882
1108	1	0.10673361983146634
1108	2	0.13137273512467829
1108	3	0.17088872798540677
1108	4	0.32508630864467136
1108	5	-0.11318978268624136
1108	6	0.19996668383117822
1108	7	-0.10139156742606303
1108	8	0.18470742833642362
1108	9	0.3066195584278013
1108	10	-0.018323418939978353
1108	11	0.3050287407778569
1108	12	0.2046043015983443
1108	13	0.006521565667021889
1108	14	0.11246517655015609
1108	15	0.057633727295524295
1108	16	-0.645507222494718
1108	17	-0.5087864480366662
1108	18	-0.21068062176743751
1108	19	-0.22227676641702487
1108	20	-0.484944027000779
1108	21	-0.19027076278376853
1108	22	-0.011284154333843333
1108	23	0.40029797559581937
1108	24	0.16623308013870997
1108	25	0.11693437124286987
1108	26	-0.17268466471767852
1108	27	-0.1021581765814048
1108	28	-0.4068922063136071
1108	29	-0.3190788741759789
1108	30	-0.3378745046828726
1108	31	-0.42755092713290566
1108	32	-0.546458899245664
1108	33	-0.4362085373501543
1108	34	0.2895517368999734
1108	35	-0.23980997556416384
1108	36	-0.1660957432716259
1108	37	0.06817099497723689
1108	38	0.15937033966130798
1108	39	0.06538537259098831
1108	40	-0.22361723642568165
1108	41	-0.8006646012643941
1108	42	0.2613412463544762
1108	43	0.18015382495165966
1109	1	0.1657777987700655
1109	2	0.056074041042990075
1109	3	0.09531217894400612
1109	4	-0.33495814814888547
1109	5	0.11034453240128528
1109	6	3.2789527880083824E-4
1109	7	0.25037281156810565
1109	8	-0.06174992092726228
1109	9	-0.1604546288464448
1109	10	-0.515128086903303
1109	11	-0.6045294698362049
1109	12	-0.33262826300293113
1109	13	-0.6514389789287023
1109	14	-0.0761983410936331
1109	15	-0.008634680595555722
1109	16	0.1431840806450424
1109	17	-0.48989231077631246
1109	18	-0.09467617608807899
1109	19	0.45763431999586857
1109	20	0.49296650370219197
1109	21	0.4990873922592982
1109	22	1.0310887270220874
1109	23	0.5950743023532539
1109	24	-0.018262613109413905
1109	25	0.06344729149849826
1109	26	-0.1528180350983348
1109	27	0.4134017011624138
1109	28	0.43737008876836114
1109	29	0.9618324900918793
1109	30	0.47707409214958574
1109	31	0.34877583029971504
1109	32	0.5281451574367466
1109	33	-8.098154829667692E-4
1109	34	0.06462814701389163
1109	35	-0.21619230398872702
1109	36	-0.7111082467448071
1109	37	-0.12340999974352229
1109	38	-0.31062132468989745
1109	39	-0.9108580283537151
1109	40	-0.4603496620994747
1109	41	-0.0717121144625068
1109	42	-0.3172917072437471
1109	43	-0.12048524145567541
1110	1	-0.24164372874296447
1110	2	0.02080907726106318
1110	3	-0.04504873095559958
1110	4	0.20729626791414546
1110	5	-0.4988815446097732
1110	6	0.19044099732480646
1110	7	-0.105136147638909
1110	8	0.028223392073122966
1110	9	-0.318988114676916
1110	10	-0.09334641197637694
1110	11	-0.32603156667189026
1110	12	0.06513511226711444
1110	13	0.1441513120866773
1110	14	-0.013865134841250182
1110	15	0.007377519175697955
1110	16	-0.34817441471649957
1110	17	-0.06429820733242053
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2208	22	-0.5092338536230727
2208	23	0.5425888449233227
2208	24	0.6036305651031668
2208	25	0.30009456807173507
2208	26	-0.0344943968117164
2208	27	-0.0630657763627056
2208	28	0.39149489046058505
2208	29	0.756782252122548
2208	30	0.4616060858037372
2208	31	0.4681571100458797
2208	32	0.47090031343886096
2208	33	0.4886137699215851
2208	34	0.260027406664868
2208	35	0.5934322332931288
2208	36	0.2674745136993977
2208	37	0.11204743018773655
2208	38	0.17946328662686284
2208	39	0.056090676035495335
2208	40	-0.18589170334749383
2208	41	0.06141850425775175
2208	42	-0.20954448355257682
2208	43	-0.11836995853659005
2209	1	0.5037349236124342
2209	2	0.6210873519579394
2209	3	0.4512740676927791
2209	4	0.8839013166122172
2209	5	1.0358034086798327
2209	6	1.0276502996503822
2209	7	0.7086185730925507
2209	8	0.41562152365730043
2209	9	0.26598505169159736
2209	10	0.38468976373422237
2209	11	0.20666571021143199
2209	12	0.4151649274132083
2209	13	0.19086170763427646
2209	14	-0.22296869278201825
2209	15	-0.23492023474996415
2209	16	-0.05837119468072416
2209	17	0.016202687121960935
2209	18	-0.16186126786333688
2209	19	-0.5405441863793641
2209	20	-0.9300403619276238
2209	21	-0.7033085724317516
2209	22	-0.44327024733688347
2209	23	-0.4538958531742807
2209	24	0.05620845316492762
2209	25	-0.34989164643060633
2209	26	0.3413671967918148
2209	27	0.5604522826392255
2209	28	0.007637708216307715
2209	29	-0.18290619563041588
2209	30	0.4411346217838812
2209	31	0.10511602926285946
2209	32	-0.14069329009022624
2209	33	0.16823880438532512
2209	34	0.03835660434890542
2209	35	-0.1177615908039158
2209	36	-5.15560742286425E-4
2209	37	-0.06351088246735515
2209	38	0.1045391068639745
2209	39	0.4117565155723
2209	40	0.3594625839422747
2209	41	0.5199930838000668
2209	42	0.2818395804121323
2209	43	0.08758537645100972
2210	1	-0.09441892254141493
2210	2	-0.1492374343668459
2210	3	-0.09819601777730469
2210	4	0.1357303764412805
2210	5	0.5106068274832509
2210	6	0.10498363298371771
2210	7	0.05542199189596886
2210	8	-0.1522417242059504
2210	9	-0.3071431534366056
2210	10	0.029134208178668075
2210	11	-0.01849668295096052
2210	12	0.08101108125936207
2210	13	-0.3052750445024728
2210	14	0.12622788841456511
2210	15	0.0544472866175596
2210	16	0.09957752326799607
2210	17	0.27548473840401044
2210	18	0.07745497145290159
2210	19	0.015011369176193057
2210	20	0.13532715943989615
2210	21	-0.1648470339702115
2210	22	0.12308872702208618
2210	23	-0.33102405830248216
2210	24	-0.04088556392908771
2210	25	0.687578439039477
2210	26	0.42724753867215526
2210	27	0.6524180946050393
2210	28	0.6371077936863898
2210	29	0.6287177359935185
2210	30	0.7020576987069589
2210	31	0.3224151745620105
2210	32	0.5789648295678944
2210	33	0.2834524795989992
2210	34	0.4553822453745511
2210	35	0.184529007486681
2210	36	0.5010229007961726
2210	37	0.39495065599418666
2210	38	0.5346245769494473
2210	39	0.6839616437774296
2210	40	0.21204378052347295
2210	41	0.4423862461932302
2210	42	-0.08096383839129118
2210	43	-0.12533770047207327
2211	1	-0.1824189225414159
2211	2	0.002762565633155134
2211	3	0.33380398222269747
2211	4	-0.19226962355872246
2211	5	0.3266068274832534
2211	6	-0.183016367016279
2211	7	-0.3765780081040262
2211	8	0.04775827579405245
2211	9	0.17285684656339484
2211	10	-0.0828657918213338
2211	11	-0.20249668295095802
2211	12	-0.1109889187406381
2211	13	0.2787249554975304
2211	14	0.25422788841456523
2211	15	0.1584472866175588
2211	16	0.09957752326799607
2211	17	0.5474847384040089
2211	18	0.3894549714528992
2211	19	0.55901136917619
2211	20	-0.03267284056010311
2211	21	0.9151529660297868
2211	22	0.3870887270220891
2211	23	0.6529759416975125
2211	24	0.8231144360709095
2211	25	0.43957843903947946
2211	26	0.5152475386721562
2211	27	1.332418094605039
2211	28	1.0131077936863946
2211	29	0.6447177359935168
2211	30	0.7340576987069625
2211	31	0.4824151745620071
2211	32	0.8109648295678937
2211	33	0.5874524795990013
2211	34	0.6153822453745477
2211	35	0.7205290074866824
2211	36	0.5810229007961709
2211	37	0.5069506559941814
2211	38	0.3746245769494436
2211	39	0.17196164377742917
2211	40	-0.22795621947652478
2211	41	-0.3176137538067678
2211	42	0.04703616160870894
2211	43	-0.14933770047206707
2212	1	-0.3739149855335384
2212	2	-0.5668122375164515
2212	3	0.10596146253765681
2212	4	0.03821856541765811
2212	5	-0.4666530150364281
2212	6	-0.001221091425733789
2212	7	0.30065033835266775
2212	8	0.13626221280192397
2212	9	-0.0021352794208588932
2212	10	-0.03700752410480135
2212	11	-0.15500061995883385
2212	12	-0.09240624157528288
2212	13	0.36785881376524543
2212	14	0.4054719829027569
2212	15	0.2077071291372441
2212	16	0.2737507516144504
2212	17	0.02024064391581959
2212	18	0.37666756987810146
2212	19	0.8339090069714672
2212	20	0.4417208602272993
2212	21	0.7249167455573442
2212	22	1.0586477821401985
2212	23	1.0496373590203518
2212	24	0.9839963258346884
2212	25	0.6253422185670345
2212	26	0.6596254914280593
2212	27	0.5220243938176381
2212	28	0.37657236061552624
2212	29	0.9455681296943013
2212	30	0.7779002183919985
2212	31	0.43151753676673366
2212	32	0.12069711303246322
2212	33	0.3237674402289201
2212	34	0.38804366269738466
2212	35	-0.1415182366078085
2212	36	-0.2870085952668191
2212	37	-0.20221469833652606
2212	38	-0.0552966828930721
2212	39	0.16200888787192014
2212	40	0.14659496162583707
2212	41	0.31230750603575075
2212	42	0.24886293326225228
2212	43	0.25475678771690724
2213	1	-0.05209634189625234
2213	2	-0.003947111786196444
2213	3	-0.0051637597127864865
2213	4	0.016311021602568587
2213	5	-0.07765123703287458
2213	6	-0.32450023798402583
2213	7	-0.04999736294274015
2213	8	0.19195182418114598
2213	9	-0.39075605666241486
2213	10	-0.2837690176277867
2213	11	-0.5090773281122516
2213	12	-0.18098891874063838
2213	13	0.01937011678784728
2213	14	0.2856472432532726
2213	15	0.23806018984336674
2213	16	0.336996878106703
2213	17	0.37393635130723624
2213	18	0.7548743262916133
2213	19	0.17630169175684074
2213	20	0.37358522395602733
2213	21	0.9319271595781728
2213	22	0.5552822754091835
2213	23	1.0345243287942907
2213	24	0.6762112102644622
2213	25	0.517836503555607
2213	26	0.9009894741560274
2213	27	-0.5066141634594814
2213	28	0.10923682594445694
2213	29	0.11162096179996439
2213	30	0.23579963419083327
2213	31	0.16170549714265547
2213	32	0.478964829567893
2213	33	0.27893635056674526
2213	34	-0.12706936752867648
2213	35	-0.11624518606170398
2213	36	-0.02284806694576247
2213	37	0.25720872051031307
2213	38	-0.17537542305055354
2213	39	-0.1213286788032164
2213	40	-0.3149239614120063
2213	41	-0.3982589150970881
2213	42	-0.757931580326769
2213	43	-0.9893377004720705
2214	1	-0.009085589208076783
2214	2	-0.24653824737497132
2214	3	-0.16265130233015412
2214	4	-0.6172452333148186
2214	5	-0.22018992048422348
2214	6	-0.16747165156913013
2214	7	-0.7506267885918305
2214	8	-0.7184205859945649
2214	9	-0.2724764867699392
2214	10	-0.6911909950733666
2214	11	-0.5360901788859174
2214	12	-0.008224691098362769
2214	13	-0.48810431279515853
2214	14	0.3500978071137517
2214	15	0.2214066362110536
2214	16	0.48071573465010786
2214	17	0.4739888034446622
2214	18	0.17501594706265422
2214	19	0.3320845399079033
2214	20	0.44498569602526317
2214	21	-0.35996898518972387
2214	22	0.807511491249727
2214	23	0.7556100880389778
2214	24	0.9659437043635961
2214	25	0.6136272195272809
2214	26	0.8864995711924806
2214	27	0.9008733791578862
2214	28	0.5727825904343575
2214	29	0.2934331831479895
2214	30	0.3961064791947635
2214	31	0.2504151745620078
2214	32	0.18221686208821808
2214	33	-0.058986544791245876
2214	34	0.2647480990330848
2214	35	0.22849648716147897
2214	36	-0.09182262765911275
2214	37	-0.07789487246110127
2214	38	-0.08391200841640511
2214	39	0.0928071722327175
2214	40	-0.06184239833831384
2214	41	-0.16314220909131905
2214	42	-0.08818335058641225
2214	43	0.10009319383686943
2215	1	-0.2985592734186042
2215	2	-0.03365848699842644
2215	3	-0.28331882479484705
2215	4	-0.15276085162889785
2215	5	-0.931358084797445
2215	6	-1.314454963507508
2215	7	-0.21468327126192577
2215	8	-0.8322417242059501
2215	9	-0.5882659604541516
2215	10	-0.7849008795406327
2215	11	-0.6689528233018365
2215	12	-0.32572576084589855
2215	13	-0.7279417111691373
2215	14	-0.3084387782521034
2215	15	-0.3100439414526157
2215	16	-0.47200142410042645
2215	17	-0.6167257879117756
2215	18	0.007279532856408366
2215	19	-0.06393599924486182
2215	20	0.5036078611942827
2215	21	1.0369073519946994
2215	22	0.6275799550922656
2215	23	0.7971162925747066
2215	24	0.7145530325621365
2215	25	0.3671573864078965
2215	26	0.29728262639145697
2215	27	0.8636461647804765
2215	28	0.15451130245832445
2215	29	0.3802966833619337
2215	30	-0.297731774977251
2215	31	0.3030467535093777
2215	32	-0.012263240607545356
2215	33	-0.06005629233082033
2215	34	-0.013600210765801535
2215	35	-0.23876923812735384
2215	36	-0.5214332395547032
2215	37	-0.3320668878654658
2215	38	-0.5832701598926562
2215	39	-0.9512664263980071
2215	40	-0.42641235982740255
2215	41	-0.37024533275413773
2215	42	-0.16327962786497352
2215	43	0.019434229352491172
2216	1	-0.37493924774466336
2216	2	-0.5961317433099325
2216	3	-0.4797244730618573
2216	4	-0.7961070219327055
2216	5	-0.5535232538175592
2216	6	-1.0780407572601831
2216	7	-0.7181064633885796
2216	8	-0.7753311551002611
2216	9	-0.4106878688837625
2216	10	-0.1383454666180839
2216	11	-0.49543977238185377
2216	12	-0.8131027398788504
2216	13	-0.7645270770227981
2216	14	0.19562626239830294
2216	15	0.34335785572324795
2216	16	0.5701466289590513
2216	17	0.5959400229568601
2216	18	0.8823330202333892
2216	19	1.1857430764932673
2216	20	1.6888881350496519
2216	21	0.9164537790379157
2216	22	1.5473488896237129
2216	23	1.8613011449495502
2216	24	1.2748867937944866
2216	25	1.2802938861939523
2216	26	0.7401581077778445
2216	27	0.18542622468633851
2216	28	0.5727825904343575
2216	29	0.6999372481886397
2216	30	0.24163493447932183
2216	31	0.8520411908221703
2216	32	0.6293713336329319
2216	33	-0.026466219587987894
2216	34	-0.18240637251162894
2216	35	-0.23491814698486735
2216	36	-0.018651895951791175
2216	37	-0.7526916204285783
2216	38	-0.2952941222375429
2216	39	0.019636440525395926
2216	40	-0.15127329264726086
2216	41	0.2271016933477057
2216	42	0.4727922591696867
2216	43	0.6366785596905302
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	2.0
26	13.0
27	78.0
28	217.0
29	481.0
30	980.0
31	2054.0
32	3987.0
33	7237.0
34	10677.0
35	12556.0
36	10808.0
37	11155.0
38	17940.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.7292319498625055	95.32391123617062	1.5028458144145296	1.4440109995523438
2	98.5444219897163	0.585812591133509	0.22895295592335832	0.6408124632268297
3	0.6484619811984396	1.8788770224467608	0.5896271663362538	96.88303383001855
4	1.719021794740612	2.6412053617108358	91.70162693134145	3.9381459122071014
5	2.581057747649805	92.46530664449702	3.228240711133849	1.7253948967193196
6	96.97888287735185	1.3404448537405829	1.0155660437691056	0.6651062251384572
7	3.916352241478544	2.632218456225619	90.01087165057235	3.440557651723476
8	1.5476708193701874	4.680105395103732	2.978946560589394	90.79327722493669
9	19.096711477213134	29.01344316394009	20.19160665634873	31.69823870249805
10	24.1206415799033	20.901230462254738	19.402164181013532	35.57596377682843
11	26.69455210611817	23.830538393645188	15.356178927305987	34.11873057293066
12	26.629232166383137	22.52209616147146	20.360445632570574	30.488226039574833
13	23.70110767184262	24.01575810288813	19.26146683379806	33.02166739147118
14	34.35233624537931	26.31201954439058	14.880853404279812	24.454790805950292
15	34.60426685766909	27.325283945564312	18.06763532180497	20.002813874961628
16	22.514133688061193	24.730756440101302	31.2744621524136	21.48064771942391
17	18.338555989000447	37.933107373537126	24.577604399820938	19.150732237641492
18	21.39312391282104	28.52885500869743	19.923513762406632	30.154507316074902
19	28.613644795251314	29.445176476608374	19.52948099630288	22.411697731837428
20	24.83403471520485	26.07477711405876	22.77337904040727	26.317809130329117
21	21.5105199207009	27.065293854319883	26.77751486858093	24.646671356398286
22	22.21653769904713	25.048282918718424	26.317068491398604	26.418110890835838
23	24.3128477329411	26.410436784549468	26.71995907143314	22.556756411076293
24	23.74752190317836	27.6370147726546	26.60101042399437	22.014452900172667
25	21.279017714395344	26.12265779881051	29.914945321992708	22.68337916480143
26	20.879964187503997	25.570122146191725	28.381403082432694	25.168510583871583
27	21.044957472660997	26.69565773485963	29.087420860778924	23.171963931700454
28	23.026155912259387	26.026731470230864	29.056724435633434	21.890388181876318
29	23.336957216857453	26.456481422267697	28.986378461341687	21.220182899533157
30	22.139796636183412	24.983053015284263	28.611626271023855	24.265524077508474
31	22.543966233932338	25.5496578627614	30.722005499776174	21.18437040353009
32	21.59904839864934	27.12447559602988	28.774429550803234	22.50204645451755
33	24.248896847221335	24.25529193579331	29.466010104239942	22.02980111274541
34	20.32487049945642	25.708256059346425	30.734795676920122	23.232077764277033
35	21.992990893277398	26.055203110610865	31.416913946587538	20.5348920495242
36	22.665472916799896	27.48864871778474	29.932851569994245	19.913026795421114
37	24.476562000383705	25.06107309586238	30.108076996866405	20.35428790688751
38	22.08763941471401	25.489870050138137	30.762048500972067	21.66044203417579
39	22.064334591034086	25.034213723860077	31.507322376414916	21.394129308690925
40	21.78962447559603	25.345339199836282	32.12166172106825	20.743374603499436
41	23.68259490432825	24.13153586411542	30.728793615061907	21.45707561649442
42	22.396879196776876	26.079171196521074	30.965018865511283	20.558930741190764
43	21.84873949579832	25.841934947494984	31.854751032833224	20.454574523873475
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	8.0
9	15.0
10	43.0
11	71.0
12	71.0
13	84.0
14	97.0
15	155.5
16	214.0
17	280.0
18	346.0
19	346.0
20	958.5
21	1571.0
22	1220.0
23	869.0
24	954.5
25	1040.0
26	1040.0
27	1131.0
28	1222.0
29	1886.0
30	2550.0
31	2407.0
32	2264.0
33	2264.0
34	2618.5
35	2973.0
36	3395.5
37	3818.0
38	3742.5
39	3667.0
40	3667.0
41	3906.5
42	4146.0
43	4378.0
44	4610.0
45	5168.5
46	5727.0
47	5727.0
48	6586.5
49	7446.0
50	6918.0
51	6390.0
52	6483.0
53	6576.0
54	6576.0
55	5796.5
56	5017.0
57	4674.5
58	4332.0
59	4051.0
60	3770.0
61	3770.0
62	3029.5
63	2289.0
64	2086.0
65	1883.0
66	1635.0
67	1387.0
68	1387.0
69	1297.0
70	1207.0
71	1331.0
72	1455.0
73	996.5
74	538.0
75	538.0
76	442.5
77	347.0
78	288.5
79	230.0
80	154.0
81	78.0
82	78.0
83	52.0
84	26.0
85	19.5
86	13.0
87	6.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003837053143186033
3	0.0
4	0.0012790177143953444
5	0.0
6	0.002558035428790689
7	0.0
8	0.003837053143186033
9	0.005116070857581378
10	0.003837053143186033
11	0.010232141715162756
12	0.005116070857581378
13	0.003837053143186033
14	0.005116070857581378
15	0.0012790177143953444
16	0.003837053143186033
17	0.0
18	0.0012790177143953444
19	0.02046428343032551
20	0.007674106286372066
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0012790177143953444
33	0.0
34	0.0
35	0.0012790177143953444
36	0.0
37	0.0
38	0.0012790177143953444
39	0.0
40	0.0012790177143953444
41	0.0012790177143953444
42	0.0
43	0.002558035428790689
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	78185.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.37001982477457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.456606393576244	26.46287651083967
2	16.714462523017477	14.163842169214044
3	8.108189694207143	10.306324742597685
4	4.292571014580251	7.275052759480719
5	2.5538080719654666	5.410244931892307
6	1.4610438615027017	3.71426744260408
7	1.0112596975276964	2.999296540257083
8	0.6671295318018535	2.261303319050969
9	0.4890270776104084	1.864807827588412
>10	2.08591179400489	15.465882202468503
>50	0.09357925559211519	2.835582272814478
>100	0.05735502762097383	4.600626718680053
>500	0.009056056992785342	2.639892562511991
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGCTAGTCCCCGCGCTAGTCTTTTTTTTTTTTTTTTTTTTTTT	794	1.0155400652299034	No Hit
AGCTAGTCACCCAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTT	646	0.8262454434993926	No Hit
AGCTAGTCCTATACAGTCCGACGATCCGCTGAGCTAGTCTTTT	624	0.798107053782695	No Hit
AGCTAGACACCCAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTT	459	0.587069130907463	No Hit
GGCCAGTCACCAGCATCCACGACGCCGCGGTGGCGGGCTACCT	458	0.5857901131930677	No Hit
AGCTAGTCAGTTGTCTACAGTCCGACGATCTCCACAGCTAGTC	296	0.3785892434610219	No Hit
AGCTAATAAGCTGAGTCGACGCAGTTCCACATCAGACAACCAG	286	0.3657990663170685	No Hit
AGCTAGCCCCCGAGGTCACTTGGGAAACTCACTTGACCTCTTA	201	0.2570825605934642	No Hit
AGCTAGTCTTAATTTCTCCAGTCCGACGATCCGGATAGCTAGT	183	0.23406024173434803	No Hit
AGCTAGTCACCCACAAAAGATGGCCTAGTCGGCCATCACTGGA	173	0.22127006459039458	No Hit
AGCTAGTCAGGCAGGAACAAAGAAGACCTCCCACTAGGAAGAA	156	0.19952676344567374	No Hit
AGCTAGTCCCGCGCCGACGATCATTCTAGCTAGTCTTTTTTTT	152	0.19441069258809235	No Hit
AGCTAGTCGCCGGATTTTACATTCAATTGATGATGCAAGTGGA	142	0.1816205154441389	No Hit
AGCTAGTCCCTGTGGGGAACCTGAGTCATGTCATGTAATGTGA	136	0.17394640915776685	No Hit
AGCAAGTAGTGTGGGCCAGGGGAGTGCTAGCCGTGAGCCGGGA	132	0.16883033830018546	No Hit
AGCCTGTCTGGGCGGGATGCCCACGCGGGTCAGAAGCCGCAGC	129	0.16499328515699943	No Hit
AACCAGTCCAGGCGGTTAGCAAAAATCTCTGACATTTCATCCA	129	0.16499328515699943	No Hit
AGTTAATCGTCATGTGTTCCGTGCCGATGCACGCCAAAAAGAG	120	0.15348212572744133	No Hit
AGCTAGTCCGACAGGGAAGGGAGCCACAGAGTTAACGTTTTTC	117	0.1496450725842553	No Hit
AGCTAGTCCCCGCGCTAGTCTTTTTTTTTTTTTTTTTTTTTTC	114	0.14580801944106928	No Hit
AGCTGGTACAAGATTGATTTGCATGGTTCAACATGCAAATCAA	113	0.1445290017266739	No Hit
AGCTAGTCTAGTGCGATCGTTCTAGCTAGTCTTTTTTTTTTTT	101	0.12918078915392978	No Hit
AGCTAGTCCCGGTGCAGGGCAGCCGCATCCGGGCTCTGCTCGG	99	0.1266227537251391	No Hit
AGCTCGGCCTACGGCCAGTACCAGCACGACCTCGACGCGACGC	96	0.12278570058195305	No Hit
AGCTAGACCCCGCGCTAGTCTTTTTTTTTTTTTTTTTTTTTTT	95	0.12150668286755771	No Hit
AGCTAGTCTGTCTGGTGTCGGGGTGGACGCTGCTTCGGTGCAA	94	0.12022766515316237	No Hit
AGCTGGTACAAGATTTGCATGGTTCAACATGCAAATCAATCAA	92	0.11766962972437169	No Hit
AGCTAGTCACCCAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTG	90	0.11511159429558099	No Hit
AGCTGGTACAAGATGATTTGCATGGTTCAACATGCAAATCAAT	89	0.11383257658118566	No Hit
AGCAAGTGAGCTAAGGACACTGGGGTTGGTAAGTGGCAGCAGA	87	0.11127454115239495	No Hit
TGCTAGTTACAACGCTCGCACGAGCTAGTCTTTTTTTTTTTTT	87	0.11127454115239495	No Hit
AGCTAGTCACCCAGTCTTTTTTTTTTTTTTTTTTTTTTTTGCT	84	0.10743748800920892	No Hit
AGCTAGTCTAGTGGCGATCGTTCTAGCTAGTCTTTTTTTTTTT	83	0.10615847029481358	No Hit
AGCTAGTCCGCAAGAGTTCTACAGTCCGACGATCTGTCCAGCT	82	0.10487945258041824	Illumina DpnII expression Sequencing Primer (100% over 22bp)
GGCTAGGCAGCTTCCTTCCCAGCCACTGGCTGCCAGAAACTCA	81	0.1036004348660229	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0012790177143953444	0.0	0.0
12	0.0	0.0	0.0012790177143953444	0.0	0.0
13	0.0	0.0	0.0012790177143953444	0.0	0.0
14	0.0	0.0	0.0115111594295581	0.0	0.0
15	0.0	0.0	0.021743301144720853	0.0	0.0
16	0.0	0.0	0.024301336573511544	0.0	0.0
17	0.0	0.0	0.03197544285988361	0.0	0.0
18	0.0	0.0	0.03581249600306964	0.0	0.0
19	0.0	0.0	0.060113832576581185	0.0	0.0
20	0.0	0.0	0.07418302743492998	0.0	0.0
21	0.0	0.0	0.07929909829251135	0.0	0.0
22	0.0	0.0	0.12534373601074375	0.0	0.0
23	0.0	0.0	0.1880156040161156	0.0	0.0
24	0.0	0.0	0.19696872801688303	0.0	0.0
25	0.0	0.0	0.24301336573511542	0.0	0.0
26	0.0	0.0	0.2967321097397199	0.0	0.0
27	0.0	0.0	0.3798682611754173	0.0	0.0
28	0.0	0.0	0.44509816460957985	0.0	0.0
29	0.0	0.0	0.5103280680437424	0.0	0.0
30	0.0	0.0	0.5857901131930677	0.0	0.0
31	0.0	0.0	0.6638101937711838	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTCC	20	0.0018267923	37.019207	14
CAACGCT	20	0.0018267923	37.019207	10
GCGAAGA	20	0.0018267923	37.019207	11
ATGTGTT	20	0.0018267923	37.019207	12
CTATACC	25	1.2193908E-4	37.019207	14
CCGGATT	35	5.5197415E-7	37.019207	10
GGAAGGG	20	0.0018267923	37.019207	15
CCCCGAC	20	0.0018267923	37.019207	12
TGTCTAC	45	2.5411282E-9	37.019207	12
GGGCGGT	20	0.0018267923	37.019207	10
TGTTCCG	20	0.0018267923	37.019207	15
CCGAGGT	20	0.0018267923	37.019207	10
GTTCCGT	20	0.0018267923	37.019207	16
AAGATTT	20	0.0018267923	37.019207	10
CTCCTAT	25	1.2193908E-4	37.019207	11
TATACCT	25	1.2193908E-4	37.019207	15
TATACAG	55	1.2732926E-11	37.019207	10
TGTGTTC	20	0.0018267923	37.019207	13
CGAAGAA	20	0.0018267923	37.019207	12
CGCGCTA	130	0.0	37.019203	11
>>END_MODULE