FastQCFastQC Report
Thu 26 May 2016
SRR2099254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342926
Sequences flagged as poor quality0
Sequence length42
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCTCGTCGTGCAGGATGACCTGAAGGCCGTCGCCGCCTG19730.5753427853239474No Hit
ACTACTAGGTCCCTAGTTTTTTTTTTTTTTTTTTTTTTTTTT14550.4242897884674828No Hit
ACTGCTAGGCACCTAGTTTTTTTTTTTTTTTTTTTTTTTTTT11840.3452639928147763No Hit
ACTGCTAGTGGTGCTACACCCCGGTGGTTCTTATGCACAGGA9740.2840262913864799No Hit
ACTGCTAGAAGTGGAAGTAGTTAATAAACTGATTCCAGACAG6590.19216973924403516No Hit
ACTGCAAGCCCCGAAAGGGGAAAGGAACACGCGGGTAGATGA6420.18721240150936352No Hit
ACTGCTAGTTCAAAATTTCTCCCCAACTCCCCTCCCATTCGG5540.16155088852988692No Hit
ACTGCTAGCTGGGTGCGGCGGTCACAGGCTGAGTGCTGCGGC5460.15921802371357086No Hit
ACTGCTAGGTGGGGGAGGCTGGGAGGTCTGTGATCTCCCTGA5150.15017817255034613No Hit
ACTATTAGAGTTGCAGCCTAGACATCCTGGGCTCAGGTGATC4690.13676419985652882No Hit
ACTGCTAGGGCCAGTACGAGGAGGGGAGACTGCTGGTGATTT4260.12422505146883No Hit
ACTGCTAGGTGGGGGGAGGCTGGGAGGTCTGTGATCTCCCTG4080.1189761056321189No Hit
ACTCCTCGGGGACCCAGATCCTCGGTGTCACCACGCTCAACA4000.11664324081580282No Hit
ACGGCGAGAGCTAGAGAAGTGACCGCTGGAGCCTGCCCTCCT3920.11431037599948678No Hit
ACTGCTAGGAAGGGCACTGGCATAGCCTCATACGAGACAGCT3850.11226911928521022No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG251.4438565E-436.014458
GAATCGG251.4438565E-436.014458
GTGTCAA200.002100907736.014458
GCCTAAG200.002100907736.014458
AACTCGA200.002100907736.014458
GTCGTCG453.3960532E-936.0144468
AAACTCG356.9458474E-736.0091937
AGCGTAT200.002102413436.0091937
TAGCGTC1600.036.0091936
TAGCGTA700.036.0091936
TAGCGGG1050.036.0091936
TAGCGAG950.036.0091936
TAGCGAC2700.036.0091936
TAGCGAA551.6370905E-1136.0091936
CTAAGGC453.4015102E-936.0091935
TAGCCCG700.036.0091936
TAGTTAC750.036.0091936
AGACGTT356.9458474E-736.0091937
CTAACGC601.8189894E-1236.0091935
AGGCCGC356.9458474E-736.0091937
Produced by FastQC (version 0.11.5)