FastQCFastQC Report
Thu 26 May 2016
SRR2099253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94539
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCTCGTCGTGCAGGATGACCTGAAGGCCGTCGCCGCCTG5960.630427654195623No Hit
ACTACTAGGTCCCTAGTTTTTTTTTTTTTTTTTTTTTTTTTT4890.5172468505061403No Hit
ACTGCTAGGGCCAGTACGAGGAGGGGAGACTGCTGGTGATTT2860.302520652852262No Hit
ACTGCTAGTGGTGCTACACCCCGGTGGTTCTTATGCACAGGA2760.2919430076476375No Hit
ACTGCAAGCCCCGAAAGGGGAAAGGAACACGCGGGTAGATGA2280.24117031066543965No Hit
ACTGCTAGTTCAAAATTTCTCCCCAACTCCCCTCCCATTCGG1750.18510879108092956No Hit
ACTATTAGAGTTGCAGCCTAGACATCCTGGGCTCAGGTGATC1730.18299326204000466No Hit
ACTGCTAGCTGGGTGCGGCGGTCACAGGCTGAGTGCTGCGGC1630.17241561683538012No Hit
ACTGCTAGGGGTCAAATGACTAATGCTTCCCTTAAAGTTTAC1590.16818455875353028No Hit
ACTGCTAGGTGGGGGAGGCTGGGAGGTCTGTGATCTCCCTGA1540.16289573615121802No Hit
ACTGCTAGGCACCTAGTTTTTTTTTTTTTTTTTTTTTTTTTT1500.15866467806936818No Hit
ACTGCTAGAAGTGGAAGTAGTTAATAAACTGATTCCAGACAG1330.14068268122150646No Hit
ACTCCTCGGGGACCCAGATCCTCGGTGTCACCACGCTCAACA1200.12693174245549455No Hit
ACTGCTAGCGGTGAAACTAGCCTGGGCCAAGGAGAAGTATGC1190.1258739779350321No Hit
ACTGCTAGGAAGGGCACTGGCATAGCCTCATACGAGACAGCT1140.12058515533271982No Hit
ACTGCTAGGTGGGGGGAGGCTGGGAGGTCTGTGATCTCCCTG1140.12058515533271982No Hit
ACTGCTAGGTGTGGCGGTCAGCACGGTGTCGACGCAAACGTC1050.11106527464855773No Hit
ACTGCTAGTATACGCACGCGGAAACCGTTAACGCAAGAATAT1030.10894974560763282No Hit
AGTTCTAGAGGCGAACCCGCTCCAAGTATCTTGGACGAGCAA1000.10577645204624547No Hit
ACTGCTAATTTAAGAAGGACTCCCCAAACCAGGATGCAACAT990.104718687525783No Hit
ACGGCGAGAGCTAGAGAAGTGACCGCTGGAGCCTGCCCTCCT980.10366092300532057No Hit
ACTGCTAGCACCTGAAAGGACCACGTTTTATTTATTGTGCTT980.10366092300532057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGGG251.4357417E-436.0153548
TCCGATC251.4357417E-436.01535411
TTGTACG251.4357417E-436.01535412
AGAGTCG251.4357417E-436.0153547
GTCGTGC502.3464963E-1036.0153548
TTAAACG251.4357417E-436.01535412
TGTCGGG251.4357417E-436.0153547
AGCACCG200.00209296936.015357
AGGTATG309.903728E-636.015357
AGCGTCA200.00209296936.015357
AGCCGTG200.00209296936.015357
AGGTACC200.00209296936.015357
AGGTACA200.00209296936.015357
GGTACGG309.903728E-636.015358
CGAACCC200.00209296936.0153512
GGTCGCG200.00209296936.015358
CGAAAGG404.780304E-836.0153512
CCTAGTT750.036.0153512
GAGTCGA200.00209296936.015358
GCGAACC200.00209296936.0153511
Produced by FastQC (version 0.11.5)