FastQCFastQC Report
Thu 26 May 2016
SRR2099252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458415
Sequences flagged as poor quality0
Sequence length47
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCTAGACTGGCAACGTAAACTAGCAGTTGACGTTGCCAGTGTAC62241.3577217150398657No Hit
ACTGCTAGGGGGTAGACAGGAAAAGGAAAAAGAAAAAGGGAAAAGGA23260.5074004995473533No Hit
ACTGCCACGAAATCTCTACGACGTCCTCGTCGAGGTCGCCGACCGCA18440.40225559809343064No Hit
ACTGCTAGAGTGTTTGGGCTGAAGTGAGCTGTAATCACACCACTCCA18340.40007416860268535No Hit
ACTGCTAGACTGGCAACCGGCCGACGATCGTAAACTAGCAGTTGACG16850.3675708691905806No Hit
ACTGCTAGGACTAGGGAGAACCCCTATAGTGAGTCGTATTACCGGCC15820.3451021454359042No Hit
ACTGCTAGACTGGCAACGAACCCCTATAGTGAGTCGTATTACCGGCC15250.332667997338656No Hit
ACTGCTCGGAGATATCTATCAGGCCCCGGGCTGCTTGCCTGGATGGC11210.2445382459125465No Hit
ACTGCTAGGACTAGGGACGGCCGACGATCGTACAACTAGCAGTTGAC11120.24257495937087575No Hit
ACTCCTTGGCCTTCGCCATGCGATCCGCCTCCGCCGTGCGGGCCGGG10980.23952095808383234No Hit
ACTGCTAGAGTTCATCAGCCTTCGCCTCGGAACTAGCTCCTATTGTG9500.20723580162080213No Hit
ACTGCAAGAGGGAATGTAGATCAAATCCGGATCCACCACAGGGAGGC8580.18716665030594548No Hit
ACTGCTAGACTGGCAACCCGGCCGACGATCGTAAACTAGCAGTTGAC7810.1703696432272068No Hit
ACTGCTAGACTGGCAACGTTGCCAGTGTACGATCGTCGGACTGTAGA7710.1681882137364615No Hit
ACTGCCAGCTAGGCATTAAAGGTTGCTTCTGGTGAGCCAATTAAGAA7430.1620802111623747No Hit
ACTGCTAGACTGGCAACGTAGAACTCTGAACCCCTATAGTGAGTCGT7120.1553177797410643No Hit
ACTGCTAGACTGGCAACGACGTTGCCAGTGTACGATCGTCGGACTGT7050.15379077909754263No Hit
ACTGCTAGACTGGCAACGAGTCGTATTACCGGCCGACGATCGTAAAC6340.1383026297132511No Hit
ACTGCTAGGACTAGGGAGAGTCGTATTACCGGCCGACGATCGTACAA5980.13044948354656805No Hit
ACTGCTAGGACTAGGGACCGGCCGACGATCGTACAACTAGCAGTTGA5920.12914062585212088No Hit
ACTGCTAGGACTAGGGAGACTCCCTAGTCGTACGATCGTCGGACTGT5730.12499590981970485No Hit
ACTGCTAGGACTAGGGAGTAGAACTCTGAACCCCTATAGTGAGTCGT5400.11779719250024541No Hit
ACTGCTAGACTGGCAACGGCCGACGATCGTAAACTAGCAGTTGACGT5390.11757904955117088No Hit
ACTGCTAGATCAGGACTAAGGAGGATGAAGCTAAAAGAAGACTTTAA5290.1153976200604256No Hit
ACTGCTAGCGTATCGCAGTCCGACGATCGTACGTAGGAGGAGTCAAC5070.11059847518078596No Hit
ACTGCTAGACTGGCAACCTAGCAGTTGACGTTGCCAGTGTACGATCG4950.10798075979189163No Hit
ACTGCTAGACTGGCAACAGAACTCTGAACCCCTATAGTGAGTCGTAT4770.10405418670855011No Hit
ACTGCTAGACTGGCAACGGTAAACTAGCAGTTGACGTTGCCAGTGTA4690.10230904311595387No Hit
ACTGCTAGACTGGCAACAGTTGACGTTGCCAGTGTACGATCGTCGGA4660.1016546142687303No Hit
ACTGCTAGCCACCTGTGAACTGCTAGTTTTTTGTACGATCGTCGGAC4590.1001276136252086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGGC200.001109620741.0138667
GGCGTCT950.041.0138668
GTCTCCT200.001109620741.0138668
GAGAACG304.093021E-641.0138668
AGCGACC256.719078E-541.0138667
GTATATC200.001109620741.0138667
TAGGCCC800.041.0138666
AGATGCG256.719078E-541.0138667
TAGCACC352.5069312E-741.0138636
AGAGCGC352.5069312E-741.0138637
AACGTTG900.040.9914915
TTGGACG200.001112604240.9914915
ACGTTTA200.001112604240.9914915
TACGGTG256.740738E-540.9914915
CTACGCA200.001112604240.9914914
CACTACG200.001112604240.9914914
TATACGT459.586074E-1040.9914914
ATATCTC506.002665E-1140.98701512
GATCTTA304.114574E-640.98254430
TAAGCCG200.001114397340.97807311
Produced by FastQC (version 0.11.5)