FastQCFastQC Report
Thu 26 May 2016
SRR2099251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15419
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCTAGACTGGCAACGTAAACTAGCAGTTGACGTTGCCAGTGTAC1430.9274272002075361No Hit
ACTGCTAGGAAGTCTATGTTTCAGGTGTAAAATCGACACTCTGGCTT580.37615928400025944No Hit
ACTGCCACGAAATCTCTACGACGTCCTCGTCGAGGTCGCCGACCGCA490.31778973993125365No Hit
ACTGCTAGGGGGCCGCAGTTTTGCAGAGGGTGCAAGCTCCTTTCCAT450.29184772034502887No Hit
ACTGCTAGCAAAGCTTAATTAAGGACGGTGAGCAACATGAAGATCTC450.29184772034502887No Hit
ACTGCTAGGGGGTAGACAGGAAAAGGAAAAAGAAAAAGGGAAAAGGA440.2853622154484727No Hit
ACTGCTAGAGTGTTTGGGCTGAAGTGAGCTGTAATCACACCACTCCA390.25293469096569166No Hit
ACTGCTAGACTGGCAACCGGCCGACGATCGTAAACTAGCAGTTGACG380.24644918606913546No Hit
ACTGCTAGGACTAGGGAGAACCCCTATAGTGAGTCGTATTACCGGCC350.2269926713794669No Hit
ACTGCTAGACTGGCAACGAACCCCTATAGTGAGTCGTATTACCGGCC330.2140216615863545No Hit
ACTCCTTGGCCTTCGCCATGCGATCCGCCTCCGCCGTGCGGGCCGGG320.2075361566897983No Hit
ACTGCTCGGAGATATCTATCAGGCCCCGGGCTGCTTGCCTGGATGGC290.18807964200012972No Hit
ACTGCTAGAGCATAGGAGTTTGATAGTTAATATTATATGTCTTTCAA270.17510863220701733No Hit
ACTGCTAGTTGGTACGGGTTTGGATGGAGATCATTCAGGGTGGTATG250.16213762241390492No Hit
ACTGCTAGGGAGTTTGTGTTTAAAAGGGTTTTCTATCCACTGTCAAT240.15565211751734873No Hit
ACTGCTAGAGTTCATCAGCCTTCGCCTCGGAACTAGCTCCTATTGTG240.15565211751734873No Hit
ACTGCTAGAAGTAATGAGATTTGCAATGGAATTATGTTTCTGCTTCA230.1491666126207925No Hit
ACTGCTAGAGAAACAACGTCTCAGGGAATTGCCCCCAGGAGCGGAGC230.1491666126207925No Hit
ACTGCTAGGACTAGGGACGGCCGACGATCGTACAACTAGCAGTTGAC200.12971009793112392No Hit
ACTGCTAGAGCCCTGGACTTCCGCCCAGTTCCCCGAGGCTGCAGAGA200.12971009793112392No Hit
ACTGCAAGAGGGAATGTAGATCAAATCCGGATCCACCACAGGGAGGC200.12971009793112392No Hit
ACTGCTAGAGTTTGTCGTTTTCGGCAAAAATGATAATGCCGAAAAGG190.12322459303456773No Hit
ACTGCTAGACTGGCAACGTAGAACTCTGAACCCCTATAGTGAGTCGT190.12322459303456773No Hit
ACTGCTAGGGGTACGAAATTAAGAGAAGGATCTCTGTGCTGTAAACT190.12322459303456773No Hit
ACTGCTAGTGTGTCGTGGTTGCGGCGCCAGCACAAGCCACGCTTCAC180.11673908813801155No Hit
ACTGCTAGCACTAGGGGAATACGACTCACTATAGTGAGTCGTATTAC180.11673908813801155No Hit
ACTGCTAGGGTACGTTCGGTTCGATCAGGCTACTACAAAGTTCTGGG170.11025358324145534No Hit
ACTGCTAGACAGGTTACGGTTCGATCAGGCTACTACAAAGTTCTGGG160.10376807834489915No Hit
ACTGCTAGCGAACTGTGGTTTACCTATGGCATTAGCATAGCAAGCAG160.10376807834489915No Hit
ACTGCTAGACAGTTACAGTTTCAGTCTCGACGGGCTAGCACACATCT160.10376807834489915No Hit
ACTGCTAGTTCGTGACGGTTTCATTAAGAATTAGATTCTATTCTCTG160.10376807834489915No Hit
ACTGCTAGGACTAGGGACCGGCCGACGATCGTACAACTAGCAGTTGA160.10376807834489915No Hit
ACTGCTAGACTGGCAACCCGGCCGACGATCGTAAACTAGCAGTTGAC160.10376807834489915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAA200.00106399841.1107515
AAATTTC200.00106399841.1107516
AATTTCG256.331084E-541.1107517
GGCAACG458.112693E-1041.1107512
GGCAACA256.331084E-541.1107512
ACGTAAA200.00106399841.1107516
AGTTTTG303.778221E-641.1107517
CAACGTA200.00106399841.1107514
GCTAGGT600.040.9772764
GCTAGCT600.040.9772764
CTAGGAC650.040.9772765
CTAGCGT303.864481E-640.9772765
ACTAGGG600.040.97727610
TAGACTG850.040.9772766
GACTAGG600.040.9772769
ACTGCTC256.4546795E-540.9772721
AGCCCGG256.4546795E-540.9772727
TTTTGCA200.001081270740.97727219
TTGCAGA200.001081270740.97727221
TGGCAAC900.040.97727211
Produced by FastQC (version 0.11.5)