##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099251_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15419 Sequences flagged as poor quality 0 Sequence length 47 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.104740904079385 37.0 34.0 38.0 30.0 39.0 2 37.298333225241585 39.0 37.0 39.0 33.0 40.0 3 37.50573967183345 39.0 38.0 40.0 33.0 40.0 4 37.05181918412348 39.0 37.0 40.0 32.0 41.0 5 37.856994617030935 40.0 38.0 41.0 33.0 41.0 6 37.940009079706854 40.0 38.0 41.0 33.0 41.0 7 36.515467929178286 39.0 36.0 39.0 31.0 41.0 8 36.68350736104806 39.0 36.0 39.0 32.0 41.0 9 35.76107399961087 37.0 35.0 37.0 35.0 37.0 10 35.62059796355146 37.0 35.0 37.0 33.0 37.0 11 35.46604838186653 37.0 35.0 37.0 33.0 37.0 12 35.304883585187106 37.0 35.0 37.0 32.0 37.0 13 36.87949931902199 39.0 37.0 39.0 33.0 39.0 14 36.88598482391854 39.0 37.0 39.0 33.0 39.0 15 36.83098774239575 39.0 37.0 39.0 33.0 39.0 16 36.88235294117647 39.0 37.0 39.0 33.0 39.0 17 36.64861534470459 39.0 37.0 39.0 32.0 39.0 18 31.115960827550424 31.0 30.0 33.0 30.0 34.0 19 30.55736429081004 31.0 30.0 33.0 26.0 34.0 20 29.888449315779233 31.0 28.0 34.0 25.0 34.0 21 32.829950061612294 35.0 32.0 37.0 26.0 37.0 22 34.00428043323173 35.0 33.0 37.0 30.0 37.0 23 32.712886698229454 35.0 32.0 37.0 25.0 37.0 24 32.4346585381672 35.0 31.0 37.0 25.0 37.0 25 32.860950775017834 35.0 32.0 37.0 26.0 37.0 26 34.43712303002789 37.0 32.0 39.0 27.0 39.0 27 34.98586159932551 37.0 33.0 39.0 28.0 39.0 28 35.72702509890395 37.0 34.0 39.0 30.0 39.0 29 36.22984629353395 37.0 35.0 39.0 32.0 39.0 30 36.522472274466566 37.0 35.0 39.0 32.0 39.0 31 37.91847720345029 39.0 37.0 40.0 33.0 41.0 32 38.29580387833193 40.0 37.0 40.0 35.0 41.0 33 38.587521888579026 40.0 38.0 40.0 36.0 41.0 34 38.64109215902458 40.0 38.0 40.0 36.0 41.0 35 38.72948959076464 40.0 38.0 40.0 36.0 41.0 36 38.77449899474674 40.0 38.0 40.0 36.0 41.0 37 38.680653738893575 40.0 38.0 40.0 35.0 41.0 38 38.6107399961087 40.0 38.0 40.0 35.0 41.0 39 38.48965561968999 40.0 38.0 40.0 35.0 41.0 40 38.353719437058174 40.0 37.0 40.0 34.0 41.0 41 38.17854594980219 40.0 37.0 40.0 34.0 41.0 42 38.04209092677865 40.0 37.0 40.0 34.0 41.0 43 37.94740255528893 40.0 37.0 40.0 34.0 41.0 44 37.91043517737856 40.0 37.0 40.0 33.0 41.0 45 37.919060898890976 40.0 37.0 40.0 34.0 41.0 46 37.91815292820546 40.0 37.0 40.0 34.0 41.0 47 37.95810363836825 40.0 37.0 40.0 34.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.24392070738704774 1101 2 -1.6220262247982689 1101 3 -0.8556038601258962 1101 4 -0.4184271045073231 1101 5 -0.07098784726223073 1101 6 0.0114281734303745 1101 7 -0.3384466840344089 1101 8 -1.0949926050993852 1101 9 0.4011096794890534 1101 10 0.7127936777801054 1101 11 0.40491394638900147 1101 12 -0.2843747586067451 1101 13 -0.7130293494023476 1101 14 -0.34783213082162234 1101 15 0.3262637109189086 1101 16 0.24314499483386243 1101 17 0.049998906380636754 1101 18 0.8292308446871175 1101 19 1.330059578687095 1101 20 1.0220409033418072 1101 21 1.7076347079240364 1101 22 1.057035733779614 1101 23 1.2411102086688146 1101 24 2.455733917748745 1101 25 0.2860038937906708 1101 26 0.6596539871071201 1101 27 0.5957751106011315 1101 28 0.7272618040168766 1101 29 0.6338216301512816 1101 30 0.5931443947192747 1101 31 0.9962245018982756 1101 32 1.0849185690528884 1101 33 0.7646256202439687 1101 34 1.0551769235679842 1101 35 0.8864764649914036 1101 36 0.6937541907819522 1101 37 0.8719916667556831 1101 38 1.040018567041443 1101 39 0.2929060618293988 1101 40 0.04050095241080953 1101 41 -0.668438488934207 1101 42 0.24883573349470112 1101 43 0.7721535208567047 1101 44 0.15355391234537308 1101 45 0.42291080007194637 1101 46 -0.7097092622685324 1101 47 -1.229940878049355 1102 1 -1.0105873740537135 1102 2 -0.6998040025760446 1102 3 -1.0667149712370119 1102 4 0.29268400660378546 1102 5 0.10678993051555352 1102 6 0.6780948400970388 1102 7 0.0948866492989211 1102 8 -0.31721482732160666 1102 9 0.0899985683779363 1102 10 0.2683492333356554 1102 11 0.23824727972233717 1102 12 0.837847463615482 1102 13 1.0314150950420924 1102 14 0.507723424733932 1102 15 -0.6515140668588728 1102 16 0.1875894392783053 1102 17 0.36111001749175387 1102 18 0.8958975113537839 1102 19 1.5856151342426514 1102 20 1.7553742366751415 1102 21 2.3298569301462564 1102 22 1.7681468448907225 1102 23 2.774443542002153 1102 24 1.4001783621931878 1102 25 0.6193372271240065 1102 26 1.3929873204404544 1102 27 1.6513306661566887 1102 28 -0.2838493070942363 1102 29 -1.1106228142931585 1102 30 -0.4290778275029439 1102 31 0.36289116856494275 1102 32 -0.2484147642804473 1102 33 -0.6353743797560298 1102 34 0.4329547013457642 1102 35 0.6086986872136251 1102 36 0.4048653018930679 1102 37 0.9831027778667973 1102 38 0.4622407892636673 1102 39 -0.1182050492817126 1102 40 0.573834285744141 1102 41 0.6871170666213473 1102 42 -0.2511642665052989 1102 43 -0.0722909235877438 1102 44 0.7535539123453674 1102 45 0.21179968896083778 1102 46 -0.2874870400463081 1102 47 0.5478368997284235 1103 1 1.267190403724065 1103 2 0.8557515529795126 1103 3 -0.011159415681454732 1103 4 -0.7628715489517717 1103 5 -2.2820989583733393 1103 6 -1.8774607154585112 1103 7 -1.2384466840344146 1103 8 -1.039437049543828 1103 9 -0.076668098288728 1103 10 -0.6760952111087875 1103 11 -1.6506416091665557 1103 12 -0.5510414252734108 1103 13 -1.2463626827356862 1103 14 0.4521678691783819 1103 15 0.6262637109189058 1103 16 -0.3124105607216947 1103 17 -0.47222331584158184 1103 18 -0.2152135997573268 1103 19 -0.858829310201795 1103 20 0.9775964588973629 1103 21 0.607634707924035 1103 22 0.8792579560018297 1103 23 1.2744435420021532 1103 24 2.6779561399709664 1103 25 1.0082261160128922 1103 26 -0.0514571240039885 1103 27 0.0402195550455815 1103 28 -0.9505159737609006 1103 29 -0.888400592070937 1103 30 0.7375888391637204 1103 31 0.02955783523161415 1103 32 0.02936301349733128 1103 33 0.5868478424661916 1103 34 0.7107324791235357 1103 35 -0.3357457572308178 1103 36 -0.9284680314402678 1103 37 -0.683563888799867 1103 38 0.18446301148588873 1103 39 -0.7293161603928198 1103 40 0.07383428574414097 1103 41 0.07600595551024014 1103 42 -0.19560871094974885 1103 43 0.14993129863448473 1103 44 -1.0242238654324112 1103 45 -0.8437558665947194 1103 46 -0.4541537067129795 1103 47 -0.6188297669382408 1104 1 -2.3351487775624804 1104 2 -1.948342014271951 1104 3 -1.8766564917048427 1104 4 -1.888602543103815 1104 5 -0.6885317069113484 1104 6 -0.9359402476222556 1104 7 0.06857085982523614 1104 8 -1.8142908507134194 1104 9 -1.1146797941951618 1104 10 -1.1468554450269153 1104 11 0.036492893757419154 1104 12 0.09808138174413727 1104 13 -1.4393451388760354 1104 14 -0.8285338852075839 1104 15 0.2315268688136456 1104 16 -0.09018833849947328 1104 17 -2.434211619935148 1104 18 0.5801080376695751 1104 19 0.4774279997397244 1104 20 1.2992338857979462 1104 21 1.7918452342398226 1104 22 0.5693164355339988 1104 23 -1.9799424229101312 1104 24 -0.8600555559354639 1104 25 1.5491617885275133 1104 26 0.8140399520193995 1104 27 0.5080558123555221 1104 28 0.800946014543193 1104 29 0.7811900512039145 1104 30 0.40717948243857194 1104 31 0.933066607161436 1104 32 -0.5642042379646597 1104 33 0.36462562024397016 1104 34 0.11131727444517736 1104 35 -0.14861125430684297 1104 36 0.24112261183458372 1104 37 0.7842723685100736 1104 38 0.9242290933572335 1104 39 -0.3491992013284957 1104 40 0.6264658646915109 1104 41 0.7982281777324616 1104 42 0.33655503174031054 1104 43 0.4598728191023156 1104 44 0.19215040357344293 1104 45 1.7351915018263355 1104 46 1.6306416149244498 1104 47 1.1560240342313506 1105 1 -0.3537246289556748 1105 2 -1.774967401268853 1105 3 -0.3379568013023686 1105 4 -1.0341133790171284 1105 5 -2.063144710007329 1105 6 -0.9885718265696255 1105 7 0.4772395904753921 1105 8 -1.2910710364719336 1105 9 0.08346262066552157 1105 10 0.3794603444467697 1105 11 -0.06567428890511451 1105 12 0.8411154374716929 1105 13 -0.8542058199905824 1105 14 0.17765806525681427 1105 15 0.4203813579777318 1105 16 0.6156940144417007 1105 17 0.8382342004982846 1105 18 0.3664857466478999 1105 19 0.9418242845694458 1105 20 -1.4407041946974104 1105 21 0.22528176674756395 1105 22 -0.12727799171058507 1105 23 -0.32359567368412456 1105 24 -1.897207258721842 1105 25 -0.4787019885622712 1105 26 -0.0776009148536616 1105 27 0.3996966792285832 1105 28 -0.90803231363018 1105 29 -0.1661783698487156 1105 30 -0.18724776214347116 1105 31 -0.5096578510428955 1105 32 0.1045264121901397 1105 33 -0.2236096738736748 1105 34 -0.08992111564770511 1105 35 0.33745685714826834 1105 36 0.5290483084290116 1105 37 0.02885441185372173 1105 38 0.004724449394387875 1105 39 0.02231782653528569 1105 40 -0.132048067197033 1105 41 0.6805811189089326 1105 42 0.7978553413378364 1105 43 0.038820187523370464 1105 44 0.2045343045022321 1105 45 0.5680088392876357 1105 46 0.3334279926334318 1105 47 0.6328042199898647 1106 1 0.17274595927962366 1106 2 0.027973775201736828 1106 3 -1.055603860125899 1106 4 -1.7017604378406546 1106 5 -0.5543211805955579 1106 6 -1.2885718265696227 1106 7 0.7448866492989197 1106 8 0.7883407282339476 1106 9 -0.8488903205109466 1106 10 -0.6205396555532303 1106 11 -0.1450860536109957 1106 12 -1.4677080919400751 1106 13 0.4869706505976481 1106 14 -0.18116546415495094 1106 15 0.22626371091890718 1106 16 -0.49018833849947185 1106 17 -1.3500010936193618 1106 18 0.3458975113537832 1106 19 0.9800595786870936 1106 20 0.5387075700084729 1106 21 -1.4423652920759658 1106 22 -0.9096309328870547 1106 23 -2.658889791331184 1106 24 0.10573391774874352 1106 25 1.6860038937906694 1106 26 2.042987320440453 1106 27 0.42910844393446723 1106 28 0.6772618040168794 1106 29 0.8838216301512816 1106 30 0.059811061385943276 1106 31 -0.5537754981017216 1106 32 -0.2484147642804473 1106 33 0.16462562024396732 1106 34 -0.6281564097653529 1106 35 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-2.0380293494023505 2214 14 -3.224915464154954 2214 15 -2.1237362890810942 2214 16 -2.0589383384994733 2214 17 -2.3750010936193604 2214 18 -0.10410248864621607 2214 19 0.6863095786870943 2214 20 0.057457570008473624 2214 21 0.42013470792403496 2214 22 -0.6658809328870561 2214 23 0.06611020866881745 2214 24 1.049483917748745 2214 25 -0.5264961062093292 2214 26 -0.5132626795595456 2214 27 -0.8521415560655328 2214 28 0.264761804016878 2214 29 0.3338216301512844 2214 30 -1.2276889386140581 2214 31 -0.33502549810172155 2214 32 0.06408523571955271 2214 33 -1.1041243797560298 2214 34 -0.4594064097653501 2214 35 -0.49894020167526065 2214 36 -0.7374958092180464 2214 37 -0.8571749999109812 2214 38 -0.6349814329585541 2214 39 -0.9133439381705983 2214 40 -1.644915714255859 2214 41 -1.4830218222675384 2214 42 -1.0844975998386346 2214 43 -1.5549298124766295 2214 44 -0.7256127543212969 2214 45 -0.5937558665947194 2214 46 -2.3742925956018652 2214 47 -0.9591075447160193 2215 1 -1.1449959762042496 2215 2 -0.8736391280240738 2215 3 -1.0039909569000898 2215 4 -1.280792695905177 2215 5 -0.4559340838213686 2215 6 -2.375668600763177 2215 7 -1.5018875442494632 2215 8 -0.6648850782176652 2215 9 -0.8311483850270776 2215 10 -0.6205396555532303 2215 11 -1.2402473439335822 2215 12 -0.21770809194007512 2215 13 -0.6227067687571903 2215 14 0.570447439070854 2215 15 -1.1640588697262544 2215 16 -1.025672209467217 2215 17 -0.9435494807161362 2215 18 -0.10410248864621607 2215 19 -0.2763920342161299 2215 20 -0.3951633977334623 2215 21 -0.34397819530177287 2215 22 0.76456261550004 2215 23 -0.3008252752021505 2215 24 -2.286201566122223 2215 25 -1.810770299757717 2215 26 0.554277643021102 2215 27 0.5097536052247875 2215 28 0.06919728788784596 2215 29 -0.5532751440422672 2215 30 0.6211013839665895 2215 31 0.35751482447892613 2215 32 0.26771426797761677 2215 33 -0.24827760556247824 2215 34 -0.16202737750728602 2215 35 -0.19954504038494036 2215 36 0.2614961262658255 2215 37 0.3020991936374031 2215 38 0.3367927605898302 2215 39 0.331615739248754 2215 40 -0.6519721658687629 2215 41 -1.3953202093643142 2215 42 -0.21352985790315415 2215 43 0.2968847036524025 2215 44 0.022371116646446865 2215 45 -0.23085264078827095 2215 46 -0.7462684020534809 2215 47 -0.15467206084505136 2216 1 -0.42725404072037776 2216 2 -0.6005976533696966 2216 3 -0.7056038601258976 2216 4 0.8839538478736273 2216 5 -0.18289260916699135 2216 6 -0.7028575408553408 2216 7 -0.4765419221296483 2216 8 -0.5116592717660495 2216 9 0.41539539377476586 2216 10 0.6651746301610544 2216 11 0.6191996606747168 2216 12 0.8180061937742096 2216 13 0.4441135077405036 2216 14 -0.024022607012099684 2216 15 0.37626371091890576 2216 16 0.624097375786242 2216 17 -0.178572522190791 2216 18 0.2530403684966416 2216 19 0.7086310072585214 2216 20 0.8387075700084736 2216 21 0.964777565066889 2216 22 0.7403690671129439 2216 23 1.369681637240248 2216 24 0.5985910606058908 2216 25 1.3217181795049555 2216 26 1.6072730347261697 2216 27 1.0719655867916131 2216 28 0.6129760897311627 2216 29 -0.1304640841344309 2216 30 -0.040188938614058145 2216 31 -1.2323469266731522 2216 32 0.0015852357195527134 2216 33 0.07891133452968546 2216 34 -0.07101355262249598 2216 35 0.11266694118188525 2216 36 -0.13481723778947696 2216 37 -0.5089607141966965 2216 38 -0.36712429010140823 2216 39 -0.8642367953134524 2216 40 0.32383428574414097 2216 41 0.5125138920181769 2216 42 0.8797881144470807 2216 43 -0.46117981247662954 2216 44 0.3368872456787031 2216 45 0.6562441334052806 2216 46 0.7641002615409889 2216 47 0.28196388385541127 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 2.0 26 7.0 27 25.0 28 69.0 29 120.0 30 188.0 31 376.0 32 488.0 33 708.0 34 1222.0 35 2257.0 36 3377.0 37 2915.0 38 3012.0 39 653.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.557753421103833 98.41105130034373 0.4150723133795966 0.6161229651728387 2 0.40210130358648416 0.2853622154484727 0.1491666126207925 99.16336986834425 3 0.2853622154484727 0.25942019586224785 98.71587003048188 0.7393475582074064 4 98.5926454374473 0.2788767105519165 0.38264478889681564 0.7458330631039627 5 0.25293469096569166 0.4539853427589338 0.791231597379856 98.50184836889552 6 0.557753421103833 0.9533692197937609 94.51974836241001 3.9691289966923926 7 4.085868084830404 91.70503923730462 1.9002529346909658 2.308839743174006 8 96.21221948372033 0.6161629264496044 1.1155791931508625 2.056038396679206 9 30.451420417693605 30.53573744973408 18.951874432481517 20.060967700090803 10 30.302244130237384 26.546893241665586 19.885847710468283 23.265014917628747 11 27.31223245557141 25.703722921260862 23.874691918536776 23.10935270463095 12 29.677670406641155 28.97723587781309 23.036513392567613 18.308580322978145 13 28.44542447629548 26.733251183604644 24.930280822362022 19.891043517737856 14 25.338867630845062 30.21596731305532 19.871587003048187 24.57357805305143 15 28.218431804916012 30.669952655814253 22.73169466242947 18.37992087684026 16 27.23263506063947 29.59984434788248 19.125753939944225 24.041766651533823 17 27.693105908294964 28.672417147674945 20.234775277255334 23.399701666774757 18 49.89296140123257 23.645799545896853 12.52675964969186 13.934479403178724 19 17.705428367598415 18.814449704909528 48.05759128348142 15.422530644010637 20 12.459462965365157 17.03204047217538 57.4328706706447 13.075625891814763 21 14.884233737596473 16.823399701666773 54.160451391140796 14.131915169595954 22 16.68071859394254 25.533432777741748 19.23600752318568 38.549841105130035 23 38.43908135461269 26.44349292850655 16.679641884001555 18.4377838328792 24 23.892600038913027 27.394772683053375 26.019845644983462 22.69278163305013 25 20.824956222841948 28.23788831960568 27.569881315260393 23.367274142291976 26 23.711005901809457 24.275244827809843 27.122381477398015 24.89136779298268 27 23.659121862637004 25.176730008431157 30.384590440365784 20.779557688566054 28 23.010571372981385 27.291004604708473 28.59459108891627 21.103832933393864 29 23.328361112912642 25.929048576431672 28.97723587781309 21.765354432842596 30 23.46455671574032 24.430896945327195 30.527271548090017 21.57727479084247 31 21.04546338932486 26.88241779622544 30.832090278228158 21.240028536221544 32 21.79129645242882 27.926584084570983 30.74777871457293 19.534340748427265 33 21.732926908359815 28.400025942019585 28.549192554640378 21.31785459498022 34 20.87035475711784 27.070497438225566 29.119916985537326 22.93923081911927 35 22.634412088981126 27.93306958946754 29.353395161813346 20.079123159737986 36 23.67209287243012 26.90835981581166 28.789156235812957 20.630391075945262 37 21.149231467669757 27.427200207536156 30.001945651468965 21.421622673325118 38 24.02231013684415 26.408975938776834 30.37161943057267 19.197094493806343 39 22.926259809326154 28.088721706984888 26.79162072767365 22.193397756015308 40 23.004085868084832 26.83701926194954 28.451909981192035 21.706984888773594 41 21.8237239769116 28.59459108891627 29.016148907192424 20.5655360269797 42 23.522926259809328 26.798106232570206 29.230170568778778 20.44879693884169 43 23.458071210843766 27.53096828588106 29.029119916985536 19.981840586289643 44 23.41419120508497 26.1058503048385 30.101180438448566 20.378778051627965 45 20.9508983589544 27.346435752740483 30.57014983459817 21.132516053706947 46 21.60321681042869 28.432453466502366 29.029119916985536 20.935209806083403 47 21.747308340900247 27.24088727461409 28.784537553508887 22.22726683097678 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 4.0 8 7.0 9 7.0 10 6.0 11 5.0 12 13.5 13 22.0 14 30.5 15 39.0 16 44.5 17 50.0 18 73.0 19 96.0 20 94.0 21 92.0 22 97.5 23 103.0 24 123.5 25 144.0 26 144.0 27 193.0 28 242.0 29 296.0 30 350.0 31 399.0 32 448.0 33 495.5 34 543.0 35 609.5 36 676.0 37 744.5 38 813.0 39 849.5 40 886.0 41 1028.0 42 1170.0 43 1170.0 44 1150.0 45 1130.0 46 1209.0 47 1288.0 48 1403.5 49 1519.0 50 1415.0 51 1311.0 52 1307.5 53 1304.0 54 1168.0 55 1032.0 56 860.5 57 689.0 58 689.0 59 579.0 60 469.0 61 433.0 62 397.0 63 297.5 64 198.0 65 152.0 66 106.0 67 86.0 68 66.0 69 65.5 70 65.0 71 57.0 72 49.0 73 64.0 74 79.0 75 79.0 76 52.0 77 25.0 78 15.0 79 5.0 80 2.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.006485504896556197 9 0.006485504896556197 10 0.006485504896556197 11 0.006485504896556197 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.025942019586224788 19 0.0 20 0.006485504896556197 21 0.0 22 0.0 23 0.03242752448278098 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.006485504896556197 45 0.012971009793112394 46 0.0 47 0.006485504896556197 >>END_MODULE >>Sequence Length Distribution pass #Length Count 47 15419.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.16914196770219 #Duplication Level Percentage of deduplicated Percentage of total 1 80.32537341832366 54.75711784162397 2 11.740081819046713 16.006226084700696 3 4.014841594520027 8.210649199040146 4 1.4556179240795357 3.9691289966923926 5 0.6659689848729902 2.2699267137946686 6 0.3900675482827514 1.5954342045528245 7 0.32347064979545237 1.5435501653803747 8 0.2283322233850252 1.2452169401387898 9 0.20930453810293978 1.284129969518127 >10 0.6279136143088193 7.8150334003502175 >50 0.009513842641042717 0.37615928400025944 >100 0.009513842641042717 0.9274272002075361 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTGCTAGACTGGCAACGTAAACTAGCAGTTGACGTTGCCAGTGTAC 143 0.9274272002075361 No Hit ACTGCTAGGAAGTCTATGTTTCAGGTGTAAAATCGACACTCTGGCTT 58 0.37615928400025944 No Hit ACTGCCACGAAATCTCTACGACGTCCTCGTCGAGGTCGCCGACCGCA 49 0.31778973993125365 No Hit ACTGCTAGGGGGCCGCAGTTTTGCAGAGGGTGCAAGCTCCTTTCCAT 45 0.29184772034502887 No Hit ACTGCTAGCAAAGCTTAATTAAGGACGGTGAGCAACATGAAGATCTC 45 0.29184772034502887 No Hit ACTGCTAGGGGGTAGACAGGAAAAGGAAAAAGAAAAAGGGAAAAGGA 44 0.2853622154484727 No Hit ACTGCTAGAGTGTTTGGGCTGAAGTGAGCTGTAATCACACCACTCCA 39 0.25293469096569166 No Hit ACTGCTAGACTGGCAACCGGCCGACGATCGTAAACTAGCAGTTGACG 38 0.24644918606913546 No Hit ACTGCTAGGACTAGGGAGAACCCCTATAGTGAGTCGTATTACCGGCC 35 0.2269926713794669 No Hit ACTGCTAGACTGGCAACGAACCCCTATAGTGAGTCGTATTACCGGCC 33 0.2140216615863545 No Hit ACTCCTTGGCCTTCGCCATGCGATCCGCCTCCGCCGTGCGGGCCGGG 32 0.2075361566897983 No Hit ACTGCTCGGAGATATCTATCAGGCCCCGGGCTGCTTGCCTGGATGGC 29 0.18807964200012972 No Hit ACTGCTAGAGCATAGGAGTTTGATAGTTAATATTATATGTCTTTCAA 27 0.17510863220701733 No Hit ACTGCTAGTTGGTACGGGTTTGGATGGAGATCATTCAGGGTGGTATG 25 0.16213762241390492 No Hit ACTGCTAGGGAGTTTGTGTTTAAAAGGGTTTTCTATCCACTGTCAAT 24 0.15565211751734873 No Hit ACTGCTAGAGTTCATCAGCCTTCGCCTCGGAACTAGCTCCTATTGTG 24 0.15565211751734873 No Hit ACTGCTAGAAGTAATGAGATTTGCAATGGAATTATGTTTCTGCTTCA 23 0.1491666126207925 No Hit ACTGCTAGAGAAACAACGTCTCAGGGAATTGCCCCCAGGAGCGGAGC 23 0.1491666126207925 No Hit ACTGCTAGGACTAGGGACGGCCGACGATCGTACAACTAGCAGTTGAC 20 0.12971009793112392 No Hit ACTGCTAGAGCCCTGGACTTCCGCCCAGTTCCCCGAGGCTGCAGAGA 20 0.12971009793112392 No Hit ACTGCAAGAGGGAATGTAGATCAAATCCGGATCCACCACAGGGAGGC 20 0.12971009793112392 No Hit ACTGCTAGAGTTTGTCGTTTTCGGCAAAAATGATAATGCCGAAAAGG 19 0.12322459303456773 No Hit ACTGCTAGACTGGCAACGTAGAACTCTGAACCCCTATAGTGAGTCGT 19 0.12322459303456773 No Hit ACTGCTAGGGGTACGAAATTAAGAGAAGGATCTCTGTGCTGTAAACT 19 0.12322459303456773 No Hit ACTGCTAGTGTGTCGTGGTTGCGGCGCCAGCACAAGCCACGCTTCAC 18 0.11673908813801155 No Hit ACTGCTAGCACTAGGGGAATACGACTCACTATAGTGAGTCGTATTAC 18 0.11673908813801155 No Hit ACTGCTAGGGTACGTTCGGTTCGATCAGGCTACTACAAAGTTCTGGG 17 0.11025358324145534 No Hit ACTGCTAGACAGGTTACGGTTCGATCAGGCTACTACAAAGTTCTGGG 16 0.10376807834489915 No Hit ACTGCTAGCGAACTGTGGTTTACCTATGGCATTAGCATAGCAAGCAG 16 0.10376807834489915 No Hit ACTGCTAGACAGTTACAGTTTCAGTCTCGACGGGCTAGCACACATCT 16 0.10376807834489915 No Hit ACTGCTAGTTCGTGACGGTTTCATTAAGAATTAGATTCTATTCTCTG 16 0.10376807834489915 No Hit ACTGCTAGGACTAGGGACCGGCCGACGATCGTACAACTAGCAGTTGA 16 0.10376807834489915 No Hit ACTGCTAGACTGGCAACCCGGCCGACGATCGTAAACTAGCAGTTGAC 16 0.10376807834489915 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.006485504896556197 0.0 0.0 17 0.0 0.0 0.006485504896556197 0.0 0.0 18 0.0 0.0 0.05836954406900577 0.0 0.0 19 0.0 0.0 0.08431156365523056 0.0 0.0 20 0.0 0.0 0.12322459303456774 0.0 0.0 21 0.0 0.0 0.12322459303456774 0.0 0.0 22 0.0 0.0 0.13619560282768015 0.0 0.0 23 0.0 0.0 0.1751086322070173 0.0 0.0 24 0.0 0.0 0.25293469096569166 0.0 0.0 25 0.0 0.0 0.29184772034502887 0.0 0.0 26 0.0 0.0 0.395615798689928 0.0 0.0 27 0.0 0.0 0.6874635190349568 0.0 0.0 28 0.0 0.0 0.7652895777936313 0.0 0.0 29 0.0 0.0 0.8755431610350866 0.0 0.0 30 0.0 0.0 0.9274272002075361 0.0 0.0 31 0.0 0.0 1.1219923471042221 0.0 0.0 32 0.0 0.0 1.284129969518127 0.0 0.0 33 0.0 0.0 1.4657241066217006 0.0 0.0 34 0.0 0.0 1.712173292690836 0.0 0.0 35 0.0 0.0 1.8289123808288474 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGTAA 20 0.001063998 41.11075 15 AAATTTC 20 0.001063998 41.11075 16 AATTTCG 25 6.331084E-5 41.11075 17 GGCAACG 45 8.112693E-10 41.11075 12 GGCAACA 25 6.331084E-5 41.11075 12 ACGTAAA 20 0.001063998 41.11075 16 AGTTTTG 30 3.778221E-6 41.11075 17 CAACGTA 20 0.001063998 41.11075 14 GCTAGGT 60 0.0 40.977276 4 GCTAGCT 60 0.0 40.977276 4 CTAGGAC 65 0.0 40.977276 5 CTAGCGT 30 3.864481E-6 40.977276 5 ACTAGGG 60 0.0 40.977276 10 TAGACTG 85 0.0 40.977276 6 GACTAGG 60 0.0 40.977276 9 ACTGCTC 25 6.4546795E-5 40.977272 1 AGCCCGG 25 6.4546795E-5 40.977272 7 TTTTGCA 20 0.0010812707 40.977272 19 TTGCAGA 20 0.0010812707 40.977272 21 TGGCAAC 90 0.0 40.977272 11 >>END_MODULE