FastQCFastQC Report
Thu 26 May 2016
SRR2099249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13315
Sequences flagged as poor quality0
Sequence length47
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGACTATGTGCTGAGCGCTCGCAGCTTCTCGCTCTCGCCTGCCT440.33045437476530226No Hit
ACGTGACTTGTGCATCTGCTGAGTCTAGAAGGATCATGCAGATTGTT340.25535110777318815No Hit
ACGTGAACGATACCCTACAGGTGACGGCGACGTCGGTGCGCTTGGAC290.21779947427713106No Hit
ACGTGACTCAATGGCAAGGGCATGATGGCGCATGCCTGTAATCCCAG240.18024784078107398No Hit
ACGTGACTGTAATTCTAGAGAGGTGGCGCGGCGTCTGGAAGAGGTGG230.17273751408186255No Hit
ACGTGACTAGCGAGACTGTTTCAGAGGGTCTCCAGGAAGGCCCTCCT220.16522718738265113No Hit
ACGTGACTGTAATTCTAGGAGAGGTGGCGCGGCGTCTGGAAGAGGTG220.16522718738265113No Hit
ACGTGACTAGTTGTCTCGCGATCCAGTCATGGGCCATCGAACGTGAC220.16522718738265113No Hit
ACGTGACTAAGCTCCCTAGTGACGAGGGGTCGAACGTGACTTTTTTT190.1426962072850169No Hit
ACGTGACTTATTTGTGTCCGACGATCCAGTATTGCCCAGTCGAACGT190.1426962072850169No Hit
ACGTGACTCGATAATAAAAGTTGTGTAGTGCGATCGGTGGATGCCTT180.1351858805858055No Hit
ACGTGACTCACTGATGGGGAATCAGGGCCCTGAACTGTTAAGGTGGA180.1351858805858055No Hit
ACGTGACTGGGATCATAGCTGCAATCTCATCACTGGAATGTTCCAGC170.12767555388659407No Hit
ACGTGACCAACAATAAACGCTCGACTCGGGTCACGACGCTGTTCACG170.12767555388659407No Hit
ACGTCACTTGACCAAAACAGGTCCAGGTAGCGGTAGCGCAGGCGGGC160.12016522718738265No Hit
ACGAGACGCAAACCGTTCGCAGGTCATCCTTGCTTCCGGGGCCCTGG160.12016522718738265No Hit
ACGTGACCAACAATAAAGGGTCACGACGCTGTTCACGCGGATGTCGA160.12016522718738265No Hit
ACGTGACTGATAGAGTGAACCAAAAACTCAAATAGTGTGCAGACATT150.11265490048817123No Hit
ACGTGACTACATAGCAGGCTAATGGACCGATTGATATCAGTGATGAT140.10514457378895982No Hit
ACGTGACTGACGAAAAATCCGACAATCCAGTGATCGAGGCTCGAACT140.10514457378895982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAATA303.8096532E-641.06
ACTAAGC303.8096532E-641.06
ACTAAAA303.8096532E-641.06
TGACTTG750.041.04
TGACTTC750.041.04
TGACTGC504.9112714E-1141.04
TGACTGA700.041.04
ACTGAAG200.001072973741.06
TGACTCT700.041.04
TGACTCG600.041.04
TGACTCC504.9112714E-1141.04
TGACTAC401.362605E-841.04
CTAAGCA256.385715E-541.07
ACTCTAT200.001072973741.06
CTCTATA200.001072973741.07
ACTTCTA256.385715E-541.06
CTAATAG256.385715E-541.07
GTGACTC2600.041.03
GTGACTA3750.041.03
GACTGAA401.362605E-841.05
Produced by FastQC (version 0.11.5)