FastQCFastQC Report
Thu 26 May 2016
SRR2099248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2319119
Sequences flagged as poor quality0
Sequence length47
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCA352.5291956E-740.9799510
TCTGACG431450.040.8885543
TCTGACA1060250.040.77533
TCTGACC372600.040.7157063
TGACACG35250.040.4838565
CTGACAA567550.040.4618454
CTGACGG98800.040.4470374
CTGACTA143300.040.3491824
CTGACCG77200.040.237144
TGACTCG11850.040.1422235
CTGACGT73150.040.138444
CTGACGA190000.040.1224634
CTGACGC80250.040.0875664
CTGACAC176850.040.0798534
CTGACCA180600.040.0196384
TGACAAA301900.040.0022055
CTGACAG194700.039.8594864
TGACGCG17050.039.6845365
CTGACAT145600.039.6836284
TGACACA94950.039.6037335
Produced by FastQC (version 0.11.5)