##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099247_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25510 Sequences flagged as poor quality 0 Sequence length 47 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.50121520972168 38.0 34.0 39.0 27.0 40.0 2 29.577107016856136 32.0 27.0 34.0 18.0 37.0 3 33.54743237945904 34.0 32.0 38.0 27.0 39.0 4 36.27444139553116 38.0 37.0 39.0 32.0 39.0 5 37.14104272834183 39.0 38.0 40.0 32.0 40.0 6 37.551862014896116 40.0 38.0 41.0 32.0 41.0 7 34.911289690317524 37.0 33.0 39.0 28.0 39.0 8 37.17902783222266 39.0 37.0 39.0 33.0 40.0 9 35.529439435515485 37.0 35.0 37.0 33.0 37.0 10 35.577616620932965 37.0 35.0 37.0 33.0 37.0 11 35.51897295178362 37.0 35.0 37.0 33.0 37.0 12 35.501842414739315 37.0 35.0 37.0 33.0 37.0 13 37.132261858094864 39.0 37.0 39.0 34.0 39.0 14 37.06056448451588 39.0 37.0 39.0 33.0 39.0 15 37.011603292826344 39.0 37.0 39.0 33.0 39.0 16 36.98823990591925 39.0 37.0 39.0 33.0 39.0 17 36.88741669933359 39.0 37.0 39.0 33.0 39.0 18 31.18451587612701 31.0 30.0 31.0 30.0 34.0 19 30.950686005488045 31.0 30.0 33.0 28.0 34.0 20 30.56656213249706 31.0 30.0 33.0 27.0 34.0 21 33.68024304194434 35.0 33.0 37.0 28.0 37.0 22 34.10513524108193 35.0 33.0 37.0 30.0 37.0 23 32.897295178361425 35.0 32.0 37.0 26.0 37.0 24 32.096942375539 35.0 30.0 37.0 25.0 37.0 25 32.00035280282242 35.0 30.0 37.0 25.0 37.0 26 33.04735397883183 35.0 30.0 39.0 25.0 39.0 27 33.14315954527636 35.0 30.0 39.0 25.0 39.0 28 33.38008624068993 35.0 30.0 39.0 25.0 39.0 29 33.52355938847511 35.0 31.0 39.0 25.0 39.0 30 33.51015288122305 35.0 31.0 39.0 25.0 39.0 31 34.75574284594277 36.0 32.0 40.0 27.0 41.0 32 35.154762838102705 37.0 32.0 40.0 27.0 41.0 33 35.586201489611916 37.0 33.0 40.0 27.0 41.0 34 35.9296354370835 37.0 34.0 40.0 30.0 41.0 35 36.40568404547236 38.0 34.0 40.0 31.0 41.0 36 36.96358290866327 38.0 35.0 40.0 32.0 41.0 37 37.31172089376715 39.0 36.0 40.0 32.0 41.0 38 37.56068208545668 39.0 36.0 40.0 33.0 41.0 39 37.79921599372795 39.0 36.0 40.0 34.0 41.0 40 37.9000392003136 39.0 37.0 40.0 34.0 41.0 41 37.94018032144257 40.0 37.0 40.0 34.0 41.0 42 37.78365346922775 39.0 36.0 40.0 34.0 41.0 43 37.565503724029796 39.0 36.0 40.0 33.0 41.0 44 37.39000392003136 39.0 36.0 40.0 33.0 41.0 45 37.199647197177576 39.0 36.0 40.0 33.0 41.0 46 37.03986671893375 39.0 36.0 40.0 32.0 41.0 47 36.854958839670715 39.0 35.0 40.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -1.3017733740613622 1101 2 0.7261321528683027 1101 3 0.16760824511449357 1101 4 0.5289557690331605 1101 5 -0.07398336253310589 1101 6 0.07033071370416621 1101 7 1.3966613186695866 1101 8 0.8279293534371206 1101 9 0.03930671591723467 1101 10 0.8509046309521864 1101 11 0.5975963107684734 1101 12 0.14219830974028014 1101 13 0.2952473049257094 1101 14 -0.2780883335402393 1101 15 0.5588887005774978 1101 16 0.4051280517072726 1101 17 0.3566770613602017 1101 18 -0.40235334942116197 1101 19 -0.07329670578467073 1101 20 -0.45830044300507566 1101 21 0.49923055934586813 1101 22 -0.2949283779580014 1101 23 -0.7492629733041056 1101 24 -2.4121092081349005 1101 25 -0.28965696539222563 1101 26 -0.7322603335096716 1101 27 0.08617156073382404 1101 28 -1.8672088502286286 1101 29 -1.5644814406575662 1101 30 -1.4705197698224879 1101 31 -2.7406604561320123 1101 32 -2.1254917091654164 1101 33 -1.3903988197459256 1101 34 -2.092805715826522 1101 35 -2.182571663457857 1101 36 -2.127597343457623 1101 37 -1.958453580232856 1101 38 -2.806298653776423 1101 39 -2.357534399522919 1101 40 -2.6946813160102963 1101 41 -0.7885734675910001 1101 42 -0.9259569030976067 1101 43 0.1104163084421188 1101 44 -0.23861477854570978 1101 45 0.10215869564746072 1101 46 -0.8859708045521586 1101 47 -1.3116532416679476 1102 1 0.4861054138174268 1102 2 1.5148299415660915 1102 3 0.0013510788573256605 1102 4 0.8966871367645268 1102 5 -0.4318867204364665 1102 6 0.2816329250063774 1102 7 0.9281928502011141 1102 8 0.36846989397766094 1102 9 0.6019602785707932 1102 10 0.18669496674252173 1102 11 0.1315848447570076 1102 12 0.5598887274307032 1102 13 0.7088427185211259 1102 14 0.5720345165826117 1102 15 1.266505408194206 1102 16 0.9129085594877822 1102 17 1.4451291498122956 1102 18 -0.169757116824929 1102 19 0.36568773319977055 1102 20 0.5523465676419335 1102 21 -0.4458963857810758 1102 22 -0.31212739515701315 1102 23 0.2548320307909009 1102 24 0.08011028408458998 1102 25 -0.263448939184201 1102 26 0.08674048549114843 1102 27 -1.409323934761673 1102 28 -0.3045552875750701 1102 29 -0.6693135454896719 1102 30 -0.32473762404033835 1102 31 0.3371446216730618 1102 32 -1.1254917091654164 1102 33 1.1304857011385963 1102 34 1.325703702682901 1102 35 0.463619982733789 1102 36 -0.7696939855542624 1102 37 0.019433397654125883 1102 38 0.40582255834478786 1102 39 0.296028254039733 1102 40 -0.5251481464771217 1102 41 0.24582456680703757 1102 42 0.8979579208172197 1102 43 1.1702033682291813 1102 44 0.495209955279023 1102 45 0.603387196875957 1102 46 0.6856917671104128 1102 47 1.0519831219684193 1103 1 0.588669516381529 1103 2 -0.5745671478309973 1103 3 0.5335756110818579 1103 4 0.5009837410611269 1103 5 0.4295131409633939 1103 6 0.5505171938906486 1103 7 0.8069177289259954 1103 8 0.5971601226678871 1103 9 0.8015444781549945 1103 10 0.8415806216281823 1103 11 1.1010928142649732 1103 12 0.7622449297869025 1103 13 0.8710048806832873 1103 14 1.0599070044550984 1103 15 0.6684458101346067 1103 16 1.1277387743179972 1103 17 1.3287050333881751 1103 18 0.13377718670937355 1103 19 0.5770529445649828 1103 20 0.5440305593259254 1103 21 0.6670627271780347 1103 22 -0.12709621012582772 1103 23 0.4488722248310921 1103 24 -1.5706173666430558 1103 25 -0.04723272296798342 1103 26 0.09990749865816184 1103 27 0.29129976586202844 1103 28 -0.5408685238883066 1103 29 0.5683856922095671 1103 30 0.68565738635467 1103 31 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0.7901844054797706 1104 21 0.7568063169216259 1104 22 0.020980712951093494 1104 23 -0.3363841854253167 1104 24 1.140921094895404 1104 25 -0.02223272296798484 1104 26 -0.43150275775209224 1104 27 1.2528382274004883 1104 28 1.3623366043168232 1104 29 0.9158215896454678 1104 30 0.20296507866236624 1104 31 1.0290365135649537 1104 32 0.724508290834585 1104 33 0.10278299843589167 1104 34 0.7534064053856042 1104 35 -0.48029893618512887 1104 36 -0.41699128285156206 1104 37 0.08700096522169076 1104 38 0.5058225583447893 1104 39 0.7629201459316306 1104 40 1.2485005021715239 1104 41 1.15460835059082 1104 42 1.3952552181145208 1104 43 0.8134466114724219 1104 44 0.7742640093330806 1104 45 1.3135223320110967 1104 46 1.1269079833266318 1104 47 0.8769831219684221 1105 1 0.7563756840877005 1105 2 -0.9716565449203927 1105 3 0.08243215993840636 1105 4 -0.04925880918141701 1105 5 0.4870321984824528 1105 6 0.3897410331144826 1105 7 -1.3420774200691525 1105 8 -0.03693551142774254 1105 9 -0.07371539710487696 1105 10 0.10561388566144103 1105 11 0.26671997989213736 1105 12 0.23556440310637328 1105 13 -0.047914038235632006 1105 14 0.03149397604207138 1105 15 0.23947838116718145 1105 16 0.18317882975804878 1105 17 0.12080482548796567 1105 18 0.12754018047236926 1105 19 0.09541746292950037 1105 20 0.33613035142571945 1105 21 0.01356307367838383 1105 22 -0.04185712488674653 1105 23 -1.5830058070469377 1105 24 -1.352322148347838 1105 25 -0.18236785810312028 1105 26 -1.940286541535876 1105 27 -0.5714860969238345 1105 28 1.2900393070195264 1105 29 -0.6422865184626403 1105 30 0.24282994352722653 1105 31 -0.12231483778639785 1105 32 0.7664001827264784 1105 33 1.9142694849223787 1105 34 1.9743523513315466 1105 35 -0.23908271996891273 1105 36 0.41949520363492354 1105 37 0.1275415057622311 1105 38 0.13555228807451414 1105 39 0.07981203782352253 1105 40 0.042419421090443166 1105 41 0.38095970194217443 1105 42 0.43849846135776005 1105 43 -0.8568236587978504 1105 44 -1.0723576122885419 1105 45 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35 -1.288632269518466 1106 36 -1.3725468384071178 1106 37 -1.301887923667195 1106 38 -0.6219552194329907 1106 39 -0.7287465207350365 1106 40 -0.4042772756062547 1106 41 -0.6092805382980657 1106 42 -0.4991892263299249 1106 43 -0.7809978329720195 1106 44 -0.5896248795558066 1106 45 0.15518899867775815 1106 46 0.40190798332663036 1106 47 -0.3924613224760236 1107 1 -0.5679486402366294 1107 2 0.9202353469714986 1107 3 0.3797294572357046 1107 4 -0.022231782154392477 1107 5 5.457119959686452E-4 1107 6 -0.7183670749936226 1107 7 -1.0177530957448297 1107 8 -0.631530106022339 1107 9 -1.3710126944021752 1107 10 -0.38087260082504315 1107 11 -0.05760434443218543 1107 12 -0.7103815428395706 1107 13 -1.7776437679653583 1107 14 -0.6171546726065742 1107 15 -0.5172783755895765 1107 16 -0.19519954862033018 1107 17 -0.960276255593115 1107 18 -0.4940814411492518 1107 19 0.3116336791457144 1107 20 -0.36657235127698584 1107 21 0.44599550611081185 1107 22 0.20138611835649556 1107 23 0.30888608484495705 1107 24 -0.21718701321270473 1107 25 1.3851997094644517 1107 26 1.546199944950608 1107 27 1.7528382274004883 1107 28 -1.2234742064939894 1107 29 -0.37201624819237367 1107 30 0.43201913271641956 1107 31 0.8506581351865776 1107 32 1.1447785611048573 1107 33 -0.19383862318572653 1107 34 -0.18780981083061477 1107 35 -0.6444881253743162 1107 36 -1.229153445013722 1107 37 0.046460424681150414 1107 38 -0.05363690111467179 1107 39 -1.0282960702845898 1107 40 -1.3359589572879358 1107 41 -0.6190402980578256 1107 42 -1.1290691062098048 1107 43 -0.6946614966356819 1107 44 -0.4237089636398963 1107 45 -0.7209371274483658 1107 46 -1.4224163409976924 1107 47 -2.1372060672207667 1108 1 -1.9710374433254287 1108 2 0.44301526975142025 1108 3 1.5511580286642754 1108 4 0.5376137776911634 1108 5 0.608267719717972 1108 6 0.21059045396390275 1108 7 0.8633279853362481 1108 8 0.5422150677228359 1108 9 0.20813788474840322 1108 10 -0.11446333441577394 1108 11 0.02270453587669863 1108 12 -0.3798796123376391 1108 13 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-0.8694501417381275 2214 2 -0.26174663501048556 2214 3 -1.709159431653184 2214 4 -1.6084179683405821 2214 5 -1.401256089805834 2214 6 -1.7195682761948277 2214 7 -2.425560903552636 2214 8 -1.7276262021184365 2214 9 -0.7950367184262035 2214 10 -0.5874792074316488 2214 11 0.24810136127351967 2214 12 -0.183054215512243 2214 13 -0.24010623042782697 2214 14 -0.8477853032372025 2214 15 -1.4734345317457311 2214 16 -0.5201244735452519 2214 17 -1.8251411204579782 2214 18 0.2158284687606553 2214 19 0.03175379926583588 2214 20 0.5457399610353271 2214 21 0.5901396502549616 2214 22 0.5293140462844264 2214 23 0.8163935923524619 2214 24 1.268698872673184 2214 25 -0.5138993896346484 2214 26 0.3101639089145749 2214 27 1.1083937829560426 2214 28 0.34289215987237753 2214 29 0.8880438118676892 2214 30 1.5112984119956963 2214 31 2.073480958009398 2214 32 -0.1477139313876421 2214 33 0.22778299843589167 2214 34 -0.5493713723921729 2214 35 0.2335899527037597 2214 36 0.28856427270399365 2214 37 0.36477874299946933 2214 38 0.11693366945589645 2214 39 0.4101423681538492 2214 40 -0.009832831161808997 2214 41 0.3851639061463743 2214 42 -0.07141144855214776 2214 43 -0.1198867218609081 2214 44 -0.38406932400025084 2214 45 -0.083699890211129 2214 46 0.23524131665996606 2214 47 0.007538677523974968 2215 1 0.5111054138174254 2215 2 0.09658669832284872 2215 3 -2.220270542764297 2215 4 -0.9695290794516893 2215 5 -0.4345894231391654 2215 6 -0.9001238317503777 2215 7 -1.436672014663749 2215 8 -0.1720706465628794 2215 9 -1.6561478295373107 2215 10 -1.1680347629872045 2215 11 -0.5880097498375889 2215 12 -1.494165326623353 2215 13 -0.7039951193167155 2215 14 -1.1394519699038739 2215 15 -1.8706567539679568 2215 16 -2.147902251323032 2215 17 -0.900141120457981 2215 18 0.6241618020939903 2215 19 0.587309354821393 2215 20 0.7651844054797721 2215 21 -0.5931936830783755 2215 22 0.6459807129510935 2215 23 1.2886158145746833 2215 24 1.0659210948954012 2215 25 1.302767277032018 2215 26 2.418497242247909 2215 27 1.9778382274004898 2215 28 1.2123366043168247 2215 29 1.890821589645462 2215 30 1.402965078662362 2215 31 1.829036513564958 2215 32 1.3245082908345864 2215 33 0.8527829984358917 2215 34 0.8284064053856 2215 35 0.24470106381487255 2215 36 -0.3419912828515592 2215 37 -0.11299903477831208 2215 38 -0.24417744165521071 2215 39 0.03792014593162918 2215 40 -0.3014994978284733 2215 41 -0.5703916494091814 2215 42 -1.054744781885482 2215 43 -1.7865533885275795 2215 44 -2.1757359906669222 2215 45 -1.0364776679889047 2215 46 -1.4230920166733725 2215 47 -0.2730168780315836 2216 1 0.43347383487005686 2216 2 2.7979024877965344 2216 3 -1.357112648027453 2216 4 0.10152355212725439 2216 5 -1.2924841599812709 2216 6 -2.0014396212240655 2216 7 0.4054332484941483 2216 8 0.35424514291080555 2216 9 0.4938521704626879 2216 10 0.3517020791180556 2216 11 0.37909551332030844 2216 12 -0.33627058978125035 2216 13 0.4499522491043422 2216 14 0.375021714306655 2216 15 0.5780274565583596 2216 16 -0.6084285671125045 2216 17 0.04327993217360415 2216 18 0.43337232840978146 2216 19 0.5399409337687615 2216 20 0.49544756337450835 2216 21 0.23049052744794096 2216 22 1.0854543971616195 2216 23 1.4070368672062656 2216 24 0.39092109489540405 2216 25 -0.1524958808627197 2216 26 0.027707768563693946 2216 27 -0.11558282523109398 2216 28 1.2189155516852423 2216 29 0.6237163264875676 2216 30 1.3253334997149935 2216 31 0.4132470398807442 2216 32 1.190297764518796 2216 33 0.6119935247516821 2216 34 -0.39264622619334233 2216 35 1.2552273796043423 2216 36 0.24879819083265176 2216 37 0.27121149153747837 2216 38 0.5110857162395277 2216 39 1.2405517248789977 2216 40 0.8077110284873115 2216 41 1.003292561117135 2216 42 0.9057815339039905 2216 43 0.22923608515663574 2216 44 0.8650534830172916 2216 45 0.5806275951689912 2216 46 1.2176974570108428 2216 47 0.9993515430210493 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 1.0 25 4.0 26 17.0 27 63.0 28 154.0 29 293.0 30 525.0 31 870.0 32 1554.0 33 2792.0 34 4235.0 35 4918.0 36 4009.0 37 2694.0 38 3177.0 39 204.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 98.81615052920424 0.5096040768326147 0.3253626029008232 0.3488827910623285 2 0.7604860838886711 97.45981967855742 0.7291258330066641 1.0505684045472363 3 0.36065702301148617 1.1917362499509978 97.93406248774942 0.5135442392880945 4 0.058800470403763225 0.18816150529204234 0.2352018816150529 99.51783614268915 5 0.4900039200313603 0.5448843590748726 97.96550372402979 0.999607996863975 6 98.82399059192474 0.5801646413171305 0.33320266562132494 0.2626421011368091 7 0.7918463347706782 96.66013328106625 1.2387299098392788 1.3092904743237945 8 0.8781558726673985 0.7723067273012388 0.7997490983220951 97.54978830170927 9 19.903559667555275 47.9065391249804 14.238670221107103 17.95123098635722 10 20.937708259829865 46.873652436395 14.712454427848995 17.476184875926144 11 19.702849974518795 46.57179818887451 15.77874475675252 17.946607079854168 12 19.984319874559 46.07604860838887 16.742453939631517 17.197177577420618 13 20.917287338298706 47.3657389259114 14.402195217561742 17.314778518228145 14 18.6671893375147 48.059584476675816 15.150921207369658 18.122304978439825 15 20.603684829478635 46.80909447275578 15.664445315562524 16.922775382203056 16 18.34182673461388 48.294786358290864 16.816934535476282 16.546452371618972 17 19.976479811838495 50.18424147393179 15.088200705605646 14.751078008624068 18 46.28293601003764 24.18444165621079 14.793757841907151 14.738864491844417 19 24.371790348504447 25.36751734681877 34.466266807793325 15.794425496883452 20 17.561740493923953 23.7749901999216 41.01528812230498 17.64798118384947 21 16.448451587612702 25.75852606820855 41.74441395531164 16.048608388867112 22 17.127959855731536 28.395013329151634 21.09534263760389 33.38168417751294 23 27.693695106649933 30.728513174404014 18.557873274780427 23.019918444165622 24 24.414912383864518 28.4331020424164 25.100944764592885 22.051040809126192 25 20.61936495491964 31.19168953351627 25.43316346530772 22.75578204625637 26 19.50607604860839 28.6593492747942 26.275970207761663 25.55860446883575 27 21.975695805566446 27.30301842414739 28.57702861622893 22.144257154057232 28 20.815366522932184 28.482947863582908 31.089768718149745 19.611916895335163 29 19.357114856918855 26.464131713053707 32.99882399059192 21.179929439435515 30 18.255586044688357 26.07604860838887 34.17483339866719 21.493531948255587 31 18.612308898471188 27.710701685613486 32.72442179537436 20.952567620540965 32 20.576244609956877 27.424539396315172 33.32418659349275 18.675029400235204 33 20.752646021168168 27.793022344178752 32.896903175225404 18.557428459427676 34 20.607604860838887 26.244609956879657 33.833790670325364 19.313994511956096 35 19.658957271658174 27.91454331634653 33.806350450803606 18.620148961191692 36 19.742051824846133 28.19005057038692 33.725351836606684 18.342545768160257 37 19.847118776950214 27.64014112112897 33.9749117992944 18.537828302626423 38 18.616958720451603 28.53110666823474 35.52863695166412 17.323297659649537 39 19.47863582908663 28.353586828694628 34.09643277146217 18.07134457075657 40 18.651509212073698 29.29439435515484 34.25323402587221 17.800862406899252 41 18.63974911799294 27.84398275186202 34.90395923167385 18.612308898471188 42 19.368874950999608 28.678949431595452 33.42218737749902 18.52998823990592 43 19.73343786750294 28.714229713837714 34.44923559388475 17.1030968247746 44 19.393915634310805 27.603104908264076 34.72244001881762 18.280539438607494 45 19.233181746902932 28.418535361455227 34.636192567037796 17.712090324604045 46 18.773030184241474 26.97765582124657 36.12308898471188 18.12622500980008 47 18.871770747579287 28.3860598220236 34.68579716962641 18.056372260770708 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 3.0 6 5.0 7 8.5 8 12.0 9 12.0 10 24.5 11 37.0 12 61.0 13 85.0 14 98.0 15 111.0 16 134.0 17 157.0 18 227.0 19 297.0 20 332.5 21 368.0 22 470.0 23 572.0 24 690.0 25 808.0 26 808.0 27 906.5 28 1005.0 29 1027.0 30 1049.0 31 1145.5 32 1242.0 33 1290.0 34 1338.0 35 1435.5 36 1533.0 37 1604.5 38 1676.0 39 1794.5 40 1913.0 41 1994.0 42 2075.0 43 2075.0 44 2011.5 45 1948.0 46 1949.0 47 1950.0 48 1861.5 49 1773.0 50 1644.5 51 1516.0 52 1337.5 53 1159.0 54 1060.5 55 962.0 56 816.0 57 670.0 58 670.0 59 548.0 60 426.0 61 340.0 62 254.0 63 239.0 64 224.0 65 194.0 66 164.0 67 120.5 68 77.0 69 60.0 70 43.0 71 34.5 72 26.0 73 20.0 74 14.0 75 14.0 76 13.0 77 12.0 78 9.5 79 7.0 80 4.0 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.003920031360250882 4 0.0 5 0.0 6 0.0 7 0.0 8 0.007840062720501764 9 0.007840062720501764 10 0.003920031360250882 11 0.003920031360250882 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.023520188161505293 19 0.003920031360250882 20 0.0 21 0.0 22 0.007840062720501764 23 0.023520188161505293 24 0.003920031360250882 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.003920031360250882 37 0.0 38 0.003920031360250882 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.007840062720501764 45 0.007840062720501764 46 0.0 47 0.003920031360250882 >>END_MODULE >>Sequence Length Distribution pass #Length Count 47 25510.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.39905919247354 #Duplication Level Percentage of deduplicated Percentage of total 1 88.58705994291152 72.99490395923168 2 8.163653663177927 13.453547628381028 3 1.693625118934348 4.186593492747942 4 0.5946717411988582 1.9600156801254411 5 0.2759276879162702 1.1368090944727558 6 0.18553758325404376 0.9172873382987063 7 0.11893434823977164 0.6860054880439044 8 0.09514747859181732 0.6272050176401411 9 0.04757373929590866 0.3528028224225794 >10 0.22835394862036157 3.226185809486476 >50 0.009514747859181731 0.4586436691493532 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCTGACAGAGAACTGATTCCAGACAGCATTGGGAAAGACATAGAA 61 0.23912191297530383 No Hit GATCTGACAAAATATAATGGATCTGACTTTTTTTTTTTTTTTTTTTT 56 0.21952175617404943 No Hit GATCTGACGTGAACGAGAGGAGGAAGTCTTGACTATATCCCTAGCTA 40 0.15680125441003528 No Hit GATCTGACACCACATAAAGTGGATTCAGTGAATCTAGGAAGACAGCA 38 0.1489611916895335 No Hit GATCTGACTCGGCCGATGTCCGACGATCTCAGAAAGTCGATCATGGA 38 0.1489611916895335 No Hit GATCTGACGCTGCCCGCGGTTAAAAAGGCCAAAGATAAAAAGATTTT 36 0.14112112896903176 No Hit GATCCGATGGCCCCGTCGACATCAACGAGACGGGTGAACCCGTTGCG 35 0.13720109760878088 No Hit GATCTGACAATAAATTTGTTTCGCTCGGGTCTGCGGGTTATAGCTTT 33 0.1293610348882791 No Hit GATCTGGAACATGTCAGTCAACCCCTCAAGGGTTGGTGAAGAGATTC 28 0.10976087808702471 No Hit GATCTGACCATAAAGGGAGCCGCAGCCGCTGCTGCTCGCCGAGCTCC 27 0.10584084672677382 No Hit GATCTGACAAAATAAAAGTTTCGCTCGGGTCTGCGGGTTATAGCTTT 27 0.10584084672677382 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.003920031360250882 0.0 0.0 17 0.0 0.0 0.007840062720501764 0.0 0.0 18 0.0 0.0 0.01960015680125441 0.0 0.0 19 0.0 0.0 0.10192081536652293 0.0 0.0 20 0.0 0.0 0.199921599372795 0.0 0.0 21 0.0 0.0 0.21560172481379852 0.0 0.0 22 0.0 0.0 0.2195217561740494 0.0 0.0 23 0.0 0.0 0.23128185025480205 0.0 0.0 24 0.0 0.0 0.25480203841630733 0.0 0.0 25 0.0 0.0 0.26656213249705996 0.0 0.0 26 0.0 0.0 0.297922383379067 0.0 0.0 27 0.0 0.0 0.34104272834182675 0.0 0.0 28 0.0 0.0 0.3528028224225794 0.0 0.0 29 0.0 0.0 0.40376323010584086 0.0 0.0 30 0.0 0.0 0.4116032928263426 0.0 0.0 31 0.0 0.0 0.44688357506860055 0.0 0.0 32 0.0 0.0 0.5096040768326147 0.0 0.0 33 0.0 0.0 0.5566444531556253 0.0 0.0 34 0.0 0.0 0.5684045472363779 0.0 0.0 35 0.0 0.0 0.6036848294786358 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGACAAC 125 0.0 41.000004 5 GACAACG 55 3.6379788E-12 41.000004 6 GACAACA 55 3.6379788E-12 41.000004 6 TGACCTT 20 0.001091827 41.0 5 TGACCTA 20 0.001091827 41.0 5 TGACTTA 20 0.001091827 41.0 5 GACAGTA 20 0.001091827 41.0 6 TGACCGA 35 2.3912617E-7 41.0 5 TGACTGA 40 1.45118975E-8 41.0 5 CGCCCGC 20 0.001091827 41.0 5 TGACCCC 25 6.5549015E-5 41.0 5 TGACCCA 30 3.951871E-6 41.0 5 TGACTCG 20 0.001091827 41.0 5 TGACCAC 50 5.4569682E-11 41.0 5 TGACCAA 120 0.0 41.0 5 AGGATCT 40 1.45118975E-8 41.0 17 CCGCCCG 20 0.001091827 41.0 4 CCGCCAG 25 6.5549015E-5 41.0 8 TGACATA 60 0.0 41.0 5 TGACAGT 25 6.5549015E-5 41.0 5 >>END_MODULE