FastQCFastQC Report
Thu 26 May 2016
SRR2099246_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099246_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283004
Sequences flagged as poor quality0
Sequence length47
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACTGAAGAGGAACAGCCGACGATCAATCAGTGCAAGCTTGTTCC171931.3400581759682744No Hit
TGTACTGAGACTAGGTATCTACAGTCCGACGATCAGTCTAGAAAGGT64170.5001543253177698No Hit
TGCACTGACCGTCGTACGCTTGCACTGATTTTTTTTTTTTTTTTTTT63100.4918145227918229No Hit
TGCACTGATTCATAGGCGAAGACTGATCGTCGGACTGTAGAACTCTG54620.42571963922170153Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCACTGATACGTCTATACGATCAGTCTAAGGAGGGAGCTTGCACTG46040.3588453348547627No Hit
TGCACTGAAAAAAAGAACGACTCACTATAGTGAGTCGTATTACCGGC45460.3543246942332214No Hit
TGCACTGATTCATAGGCGTAGAACTCTGAACCCCTATAGTGAGTCGT28280.22042020133998022No Hit
TGCACTGAAAAAAAGAAAGTGAGTCGTATTACCGGCCGACGATCAGT25400.19797288239163713No Hit
TGGAATGACGAGGGGCTGCCAGGACTTCCAACAGACGGGCCTCGGGC23110.18012414614451708No Hit
TGCACTGATGTGTGTGGGAGGAGCTTGCACTGATTTTTTTTTTTTTT21140.16476955644721294No Hit
TGCACTGATGAAATGAAGTAAGATCAAAGGACATCTGGCGAGTGATG19590.15268853409654218No Hit
TGCACTGATTCATAGGCGCCTATGAAGACTGATCGTCGGACTGTAGA18430.14364725285345953No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAGC256.759909E-540.9787623
TTCGACA200.001115483840.97556311
CATAGGC44000.040.46336711
TCATAGG44150.040.2794910
CACTGAA340500.040.2477463
ACTGACG56800.040.224354
CACTGAC223450.040.192663
TGACACG9800.039.9723326
CACTGAG301600.039.9578973
CTGACCG24800.039.9436385
ATAGGCG19750.039.84689712
CTGATTC55450.039.835145
TTCATAG44900.039.7397469
TGATTCA48900.039.7185446
ACTGATA70100.039.672894
CTGACGT15100.039.6604925
ACTGAAG104050.039.638954
CGTACGC7250.039.57649613
ACTGAAT75950.039.560524
ACTGACC58200.039.538674
Produced by FastQC (version 0.11.5)