##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099246_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1283004
Sequences flagged as poor quality	0
Sequence length	47
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.4039480781042	39.0	37.0	40.0	32.0	41.0
2	37.25885344083105	39.0	36.0	41.0	31.0	41.0
3	37.712417888019054	40.0	37.0	41.0	32.0	41.0
4	30.76046995956365	32.0	27.0	36.0	18.0	38.0
5	35.31408397791433	37.0	34.0	38.0	30.0	39.0
6	36.45765172984652	39.0	36.0	39.0	31.0	40.0
7	36.865874151600465	39.0	36.0	40.0	31.0	41.0
8	27.092977106852356	29.0	24.0	34.0	13.0	37.0
9	34.9341942815455	37.0	35.0	37.0	32.0	37.0
10	34.86269957069503	37.0	35.0	37.0	32.0	37.0
11	34.48563917181864	35.0	35.0	37.0	31.0	37.0
12	34.184124133673784	35.0	35.0	37.0	30.0	37.0
13	35.684325224239366	37.0	35.0	39.0	30.0	39.0
14	35.49437414068857	37.0	35.0	39.0	30.0	39.0
15	35.71981303253926	37.0	35.0	39.0	30.0	39.0
16	35.7088800970223	37.0	35.0	39.0	30.0	39.0
17	35.54454467795892	37.0	35.0	39.0	30.0	39.0
18	31.459141202989233	31.0	31.0	34.0	30.0	34.0
19	31.485544082481425	31.0	31.0	34.0	30.0	34.0
20	31.543593005166002	31.0	31.0	34.0	30.0	34.0
21	35.31962176267572	37.0	35.0	37.0	33.0	37.0
22	35.3486731140355	35.0	35.0	37.0	33.0	37.0
23	35.08455312688035	35.0	35.0	37.0	32.0	37.0
24	35.05414090680933	35.0	35.0	37.0	32.0	37.0
25	35.11304641294961	35.0	35.0	37.0	32.0	37.0
26	36.75688384447749	38.0	35.0	39.0	33.0	39.0
27	36.92177421114821	38.0	37.0	39.0	33.0	39.0
28	37.08764119207734	39.0	37.0	39.0	34.0	39.0
29	37.203970525423145	39.0	37.0	39.0	34.0	39.0
30	37.187521628927115	39.0	37.0	39.0	34.0	39.0
31	38.46100090101044	40.0	38.0	41.0	34.0	41.0
32	38.57102549953079	40.0	38.0	41.0	35.0	41.0
33	38.62162939476416	40.0	38.0	41.0	35.0	41.0
34	38.663400893528	40.0	38.0	41.0	35.0	41.0
35	38.72721753010902	40.0	38.0	41.0	35.0	41.0
36	38.80559608543699	40.0	38.0	41.0	35.0	41.0
37	38.744795027918855	40.0	38.0	41.0	35.0	41.0
38	38.65925671315132	40.0	38.0	41.0	35.0	41.0
39	38.55707932321334	40.0	38.0	41.0	34.0	41.0
40	38.43201580041839	40.0	38.0	41.0	34.0	41.0
41	38.29492503530776	40.0	38.0	41.0	34.0	41.0
42	38.164090680933185	40.0	38.0	41.0	34.0	41.0
43	37.98510215088963	40.0	37.0	41.0	33.0	41.0
44	37.841023878335534	39.0	37.0	40.0	33.0	41.0
45	37.70701650189711	39.0	37.0	40.0	33.0	41.0
46	37.43828857899118	39.0	37.0	40.0	33.0	41.0
47	37.51447774130089	39.0	37.0	40.0	33.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8888052717424273
1101	2	0.9128892865939093
1101	3	0.8099608944343899
1101	4	0.22362378417451723
1101	5	0.33375258400681673
1101	6	0.5835563449167367
1101	7	0.7676647757919142
1101	8	0.02429905109741526
1101	9	0.6492195843535669
1101	10	0.6841273509091863
1101	11	0.732676664578932
1101	12	0.7526801648649197
1101	13	1.0783483280966948
1101	14	0.9940577248728744
1101	15	0.8793694756901616
1101	16	0.8175086947803862
1101	17	1.0620604215564597
1101	18	0.30014565684827943
1101	19	0.41718092206307844
1101	20	0.407831890711428
1101	21	0.49637933062341943
1101	22	0.44507030230427347
1101	23	0.47013350266219334
1101	24	0.38531157194901766
1101	25	0.40979811640332287
1101	26	0.5340975148949525
1101	27	0.5080463620726974
1101	28	0.44517547943832625
1101	29	0.4012618343138641
1101	30	0.5239576923262561
1101	31	0.677281071517605
1101	32	0.6031337451768053
1101	33	0.5957439274906449
1101	34	0.6184275044331002
1101	35	0.6286545364493534
1101	36	0.7229959388608762
1101	37	0.6696656909823488
1101	38	0.5953466963214566
1101	39	0.6776103493618564
1101	40	0.6837534749044849
1101	41	0.6492610772785454
1101	42	0.4836833353646952
1101	43	0.4417438168097405
1101	44	0.46401347614463617
1101	45	0.5484338336582013
1101	46	1.02602734825021
1101	47	0.5832289608560544
1102	1	0.7597911480089223
1102	2	0.4958526449087799
1102	3	0.66937006053125
1102	4	-0.21451497895133187
1102	5	0.2697251882444007
1102	6	0.5553066714157566
1102	7	0.5254224832404546
1102	8	-0.17275582599013717
1102	9	0.436313048023365
1102	10	0.5449935349759372
1102	11	0.5549462146195197
1102	12	0.48300950290118294
1102	13	0.6310178238086266
1102	14	0.5496996058896926
1102	15	0.5519969304393726
1102	16	0.6095888226782691
1102	17	0.8489781016347351
1102	18	0.3445380009212471
1102	19	0.49541687855995065
1102	20	0.440257711629382
1102	21	0.44659702524000267
1102	22	0.4148563636216096
1102	23	0.4880443060961781
1102	24	0.509179919110089
1102	25	0.34674329178116636
1102	26	0.4578272945721622
1102	27	0.30278923137575475
1102	28	0.48133933720414745
1102	29	0.5028304270571127
1102	30	0.5083335465744483
1102	31	0.46680118231275003
1102	32	0.42024133772858363
1102	33	0.4934118896930073
1102	34	0.5453196725998879
1102	35	0.4680106438504268
1102	36	0.5036844564658338
1102	37	0.5693712228208767
1102	38	0.5698004616819858
1102	39	0.47243020060039953
1102	40	0.4784514789394194
1102	41	0.5258050983196227
1102	42	0.48000217688954194
1102	43	0.4159519264398597
1102	44	0.528243623692525
1102	45	0.5397444363997863
1102	46	1.1008508475498928
1102	47	0.7549011286256757
1103	1	0.4420302237244158
1103	2	0.4144745175534865
1103	3	0.49464666520341183
1103	4	0.46312430073743016
1103	5	0.5346984392798646
1103	6	0.5440146666228003
1103	7	0.5577120901690762
1103	8	0.18605552830645067
1103	9	0.35291251687954883
1103	10	0.4311347201558462
1103	11	0.48220344876225596
1103	12	0.47349868293693476
1103	13	0.5658542778159799
1103	14	0.6900375157597551
1103	15	0.6547709946877234
1103	16	0.5808174543521645
1103	17	0.6582806507461285
1103	18	0.23009554712368896
1103	19	0.4322863647774291
1103	20	0.3755521119761447
1103	21	0.4061556499961867
1103	22	0.2629819952617751
1103	23	0.3171917357048315
1103	24	0.3626618131317869
1103	25	0.36135575411380927
1103	26	0.40442317287025986
1103	27	0.3222004260611726
1103	28	0.2649065497249836
1103	29	0.2122031699616045
1103	30	0.3854352591828487
1103	31	0.4079481461192813
1103	32	0.47173488512140693
1103	33	0.42281398552532323
1103	34	0.4797316829011393
1103	35	0.4470835127710302
1103	36	0.45871745309385403
1103	37	0.41444927009332133
1103	38	0.4143068847144207
1103	39	0.409347052609796
1103	40	0.333508689884475
1103	41	0.43718162216956813
1103	42	0.3498784112305202
1103	43	0.39785542129593665
1103	44	0.1946560554338035
1103	45	0.17631041186476892
1103	46	0.6841517950043681
1103	47	0.5406457812588243
1104	1	0.6013673748801338
1104	2	0.6051383500653102
1104	3	0.605880685077075
1104	4	-0.6247216819643278
1104	5	0.15977579131838127
1104	6	0.23510460602888372
1104	7	0.4046104969853559
1104	8	0.13133231446785842
1104	9	0.3016495771801573
1104	10	0.3442388708751807
1104	11	0.33771565339372245
1104	12	0.3853915812149822
1104	13	0.5501777470504408
1104	14	0.5720519595762426
1104	15	0.3970107520258779
1104	16	0.6427464800562959
1104	17	0.7854357769059632
1104	18	0.11885929164197861
1104	19	0.33484112823221324
1104	20	0.3685475048350746
1104	21	0.3001931939035316
1104	22	0.31122641697256626
1104	23	0.2430701508196904
1104	24	0.34477516024838906
1104	25	0.2941484578045319
1104	26	0.3278370536712174
1104	27	0.40582692213008187
1104	28	0.40690566621268687
1104	29	0.2588629926868222
1104	30	0.4032351704739199
1104	31	0.48903052022772187
1104	32	0.4074607065732323
1104	33	0.42779313177344136
1104	34	0.4279286202969601
1104	35	0.40532235440413444
1104	36	0.3877668546662889
1104	37	0.3922689083898234
1104	38	0.2806166291039389
1104	39	0.3249890655870473
1104	40	0.27110303254416834
1104	41	0.3938461486141236
1104	42	0.3456267318417545
1104	43	0.30000419832890657
1104	44	0.4052230592897743
1104	45	0.33469608823457264
1104	46	0.7237394952254235
1104	47	0.6510459193299738
1105	1	0.4314966031191645
1105	2	0.40885919174033347
1105	3	0.39070803379033947
1105	4	0.8305259491115144
1105	5	0.3327655887237526
1105	6	0.3117112414452947
1105	7	0.45641976172542087
1105	8	-0.007819643646165275
1105	9	0.23767539680536487
1105	10	0.34055515477829346
1105	11	0.39398237291518257
1105	12	0.3058799560514771
1105	13	0.4742995276209214
1105	14	0.4938293206068778
1105	15	0.47789767167849817
1105	16	0.3108416429751273
1105	17	0.5011349521629853
1105	18	0.10283348936517456
1105	19	0.1946727706747282
1105	20	0.28152141977933454
1105	21	0.22146701759520226
1105	22	0.14261297057392142
1105	23	0.1661410519997304
1105	24	0.09655337135067299
1105	25	0.19266963673246806
1105	26	0.3449224095389667
1105	27	0.2374717500512915
1105	28	0.22357535439252274
1105	29	0.20769475136362558
1105	30	0.25193065746088905
1105	31	0.2401392999064953
1105	32	0.1593497305868894
1105	33	0.28206135887094774
1105	34	0.28079661457893224
1105	35	0.2561885600659579
1105	36	0.3825950794738091
1105	37	0.42275590402843477
1105	38	0.2635306226711549
1105	39	0.2285512561349492
1105	40	0.3120513308395161
1105	41	0.31025743236110515
1105	42	0.20999888453544457
1105	43	0.14401670132151168
1105	44	0.028627656963010395
1105	45	0.032845621042135065
1105	46	0.4970759136827354
1105	47	0.36497837680037293
1106	1	0.46128409963531425
1106	2	0.5122713096018643
1106	3	0.4120931672275461
1106	4	0.1305003627965391
1106	5	0.104901177917192
1106	6	0.33596798184014887
1106	7	0.1686650979341735
1106	8	-0.0605365904677484
1106	9	0.26393335255017547
1106	10	0.2390048982297941
1106	11	0.25845486077310653
1106	12	0.39053410674573286
1106	13	0.46055830578300316
1106	14	0.3578905998312436
1106	15	0.4038595572071486
1106	16	0.4086613234203327
1106	17	0.6336377963560338
1106	18	0.1553311249155307
1106	19	0.2886248054721179
1106	20	0.23284117104380186
1106	21	0.3696574711583196
1106	22	0.1251937159581047
1106	23	0.25117899741863425
1106	24	0.18597525770174173
1106	25	0.1626080011272819
1106	26	0.2276068708785033
1106	27	0.18041655214571506
1106	28	0.22130228444594024
1106	29	0.2346197788917621
1106	30	0.3881595087724108
1106	31	0.3472819938713272
1106	32	0.3361146151971752
1106	33	0.2992156123673624
1106	34	0.3236970975206219
1106	35	0.23258290000756432
1106	36	0.30823028258803475
1106	37	0.17568348105010045
1106	38	0.29159789640360145
1106	39	0.22459223079079038
1106	40	0.28933662282049255
1106	41	0.27900557621101996
1106	42	0.23349488895821935
1106	43	0.14826585720156515
1106	44	0.024718890451843833
1106	45	0.11315466717517353
1106	46	0.5818031681340656
1106	47	0.37436764059984995
1107	1	0.4664129945472766
1107	2	0.5764125327129364
1107	3	0.5045142070843411
1107	4	0.6408916491161811
1107	5	0.495255853834216
1107	6	0.4530318680040395
1107	7	0.3762181678479024
1107	8	0.9056843462326611
1107	9	0.39671948509601407
1107	10	0.385422236592845
1107	11	0.4021029559197018
1107	12	0.48856341529456415
1107	13	0.5515545739491472
1107	14	0.7067875344685248
1107	15	0.6094953342680043
1107	16	0.6632470291819175
1107	17	0.9503479168062796
1107	18	0.1631046663146165
1107	19	0.1802430459478508
1107	20	0.15774029016919755
1107	21	0.19042467147386333
1107	22	0.16104055906938441
1107	23	0.14659743813911064
1107	24	0.18719553703304825
1107	25	0.2046555541971955
1107	26	0.23908251282472293
1107	27	0.0847195891909891
1107	28	0.20487918146147877
1107	29	0.3020031464419972
1107	30	0.3174148612739529
1107	31	0.32253457530924123
1107	32	0.29636097691199836
1107	33	0.35204927775637174
1107	34	0.3224950222569163
1107	35	0.2678314360971257
1107	36	0.20800192267338957
1107	37	0.26628027106617935
1107	38	0.2099918931066469
1107	39	0.1953073472462563
1107	40	0.22869786231522937
1107	41	0.26709000263119265
1107	42	0.1674090668928656
1107	43	0.132393528395653
1107	44	0.07573902617328088
1107	45	0.06805631464328599
1107	46	0.5093521926994455
1107	47	0.3095753186710226
1108	1	0.38912141426940394
1108	2	0.345276197462816
1108	3	0.25723826421834417
1108	4	0.15076959736783735
1108	5	0.504568756947215
1108	6	0.39919800426435614
1108	7	0.3048368298250139
1108	8	0.6778245733498309
1108	9	0.2181540302871383
1108	10	0.2972562102440506
1108	11	0.32272925175155365
1108	12	0.10108853257515449
1108	13	0.35749039561270735
1108	14	0.3808613050831795
1108	15	0.36822435740437953
1108	16	0.422035604378884
1108	17	0.5349806874259286
1108	18	0.05291456934267913
1108	19	0.07993439441233718
1108	20	-0.010776054173607719
1108	21	0.10281140015727175
1108	22	0.02774286371414547
1108	23	0.037641547630975936
1108	24	0.1381478204448996
1108	25	0.1345965516942087
1108	26	0.22307140176911844
1108	27	0.19227440756220204
1108	28	0.07106989793471996
1108	29	0.05958729120546735
1108	30	0.06638913698201065
1108	31	0.06957320920895427
1108	32	0.12132288257793533
1108	33	0.15826147693603332
1108	34	0.1907173033206604
1108	35	0.10579407617186831
1108	36	0.10455180002212217
1108	37	0.12330496618660902
1108	38	-0.01572100800795795
1108	39	0.08567280305432945
1108	40	0.04598794379963067
1108	41	-0.009393428206983856
1108	42	7.832007151407083E-4
1108	43	-0.04552844441623449
1108	44	-0.14750066587462385
1108	45	-0.11232088171364296
1108	46	0.4827232290152139
1108	47	0.19141243978852174
1109	1	0.3238716312781591
1109	2	0.0406532902742498
1109	3	0.038686264232644874
1109	4	-0.016060541619047797
1109	5	0.10120393458856825
1109	6	0.19199619296853854
1109	7	-0.06228698342849981
1109	8	0.40683082514830815
1109	9	0.10041324886495318
1109	10	0.05041413205179168
1109	11	-0.04503044532784628
1109	12	0.048559802124557905
1109	13	0.16488074785302587
1109	14	0.03126063893430597
1109	15	0.13293105747910516
1109	16	0.22148018904963607
1109	17	0.404601069548022
1109	18	-0.07229516607444353
1109	19	0.040219259245318995
1109	20	-0.13379435360736736
1109	21	-0.031039364938862946
1109	22	-0.03471296251679945
1109	23	-0.07616914606029468
1109	24	0.03250749913490836
1109	25	0.0011443558793260422
1109	26	0.04717902461018042
1109	27	0.030090867500426555
1109	28	0.018502839695742068
1109	29	0.11623000333537448
1109	30	0.10188344927722426
1109	31	-0.021047782384549407
1109	32	0.004764106318688732
1109	33	0.12420197433540636
1109	34	0.16864744784820118
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2214	47	-0.39396631784793357
2215	1	-0.4023164782271422
2215	2	-0.21421840937868097
2215	3	-0.3563328110660464
2215	4	-1.3278551097429379
2215	5	-0.12102763795488869
2215	6	-0.410650068037441
2215	7	-0.37410162471908137
2215	8	-0.29196474624789204
2215	9	-0.39764525129119477
2215	10	-0.2176892586534862
2215	11	-0.0924864995686292
2215	12	-0.40886095808486544
2215	13	-0.7448672065175757
2215	14	-0.6991243064994919
2215	15	-0.574152029604079
2215	16	-0.4209707754853227
2215	17	-1.406471443608588
2215	18	-0.4523836655782105
2215	19	-0.48143370426822685
2215	20	-0.5560021471687442
2215	21	-0.36283375549243146
2215	22	-0.33679868034289484
2215	23	-0.510826935240317
2215	24	-0.5848701500888183
2215	25	-0.6941862781277806
2215	26	-1.1227152399842666
2215	27	-0.7300081549749535
2215	28	-0.8286558228600427
2215	29	-0.8513244248631011
2215	30	-0.9934926581728902
2215	31	-1.47521823367952
2215	32	-1.2832414431398576
2215	33	-1.0983569452341229
2215	34	-0.9481359198346979
2215	35	-1.4199127298492868
2215	36	-1.078221067819264
2215	37	-1.327462166271765
2215	38	-1.6629899565044184
2215	39	-1.3576053580259995
2215	40	-1.5075164851350777
2215	41	-1.4151917605494475
2215	42	-1.4868692390708347
2215	43	-1.1281769123995105
2215	44	-0.9869748891249088
2215	45	-0.8798779982348464
2215	46	-0.6798958626160285
2215	47	-0.7822137910956144
2216	1	0.4070584643058268
2216	2	0.4737493723017465
2216	3	0.6101799971871387
2216	4	4.301910056957446
2216	5	2.000931206451959
2216	6	2.044875042759003
2216	7	1.4442841492010032
2216	8	6.4469418169474615
2216	9	0.5484000970969731
2216	10	0.5907294323853378
2216	11	1.0811467044255565
2216	12	1.6346117059950203
2216	13	1.6657692524095822
2216	14	1.6479398175189672
2216	15	1.6120963383320728
2216	16	1.4391590540444525
2216	17	1.9099558536936811
2216	18	0.14056581859766482
2216	19	0.27428081788186987
2216	20	-0.020170300316099343
2216	21	0.2188062158200239
2216	22	-0.4798870048799344
2216	23	0.06240905659476681
2216	24	-0.37706934463083286
2216	25	-0.21949935854485148
2216	26	-0.6371785438331798
2216	27	-0.7948309726067606
2216	28	-0.06554874108214648
2216	29	-0.2716521248943593
2216	30	-0.3999363220872709
2216	31	0.08514514188825473
2216	32	-0.09818838957138354
2216	33	-0.026454188655293365
2216	34	0.3896047521158721
2216	35	0.50290674090229
2216	36	0.582125757241883
2216	37	0.30447295574224853
2216	38	0.2423227561236203
2216	39	0.41986388561584675
2216	40	-0.1808646526262052
2216	41	-0.01227887138267647
2216	42	-0.4113621499371618
2216	43	0.7025297942335627
2216	44	0.6022415363334517
2216	45	0.5233659010645155
2216	46	0.7834999335552908
2216	47	0.44062958594332
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	8.0
25	70.0
26	774.0
27	3671.0
28	9770.0
29	17169.0
30	26098.0
31	36299.0
32	47726.0
33	63365.0
34	87859.0
35	130465.0
36	213275.0
37	371092.0
38	273667.0
39	1696.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.522135212677153	0.5329691870855907	98.72011011682766	0.22478548340959986
2	97.8788046481575	0.23530732196261425	1.4922022785605333	0.3936857513193656
3	1.087606322988057	0.15105210362267837	0.9621972235407455	97.79914434984852
4	0.2611088161248334	97.28571539918471	1.1748337867013772	1.2783419979890724
5	0.14512905730025658	0.5089649539047667	0.2756049122522595	99.07030107654272
6	0.41528155044680953	0.22759424311274098	99.14963931768493	0.20748488875551938
7	98.48539881432106	0.5326669670095668	0.7584697477275526	0.22346447094182442
8	2.0856829970732313	94.1884745339969	2.4314391828431354	1.2944032860867432
9	26.944783113010256	28.531936120738138	24.58725987358627	19.936020892665336
10	28.950459610907192	27.849077075731937	24.532613940088844	18.667849373272023
11	21.45516106101987	35.26187224861885	19.211031713369998	24.071934976991283
12	27.779609429498947	34.76116430695365	20.800236945584356	16.658989317963048
13	27.088613909231775	32.61790298393458	20.385517114521857	19.907965992311794
14	25.56769156301955	36.29765253605142	19.744590435080152	18.39006546584887
15	30.10561184723305	32.07131722525332	20.50444271239283	17.31862821512081
16	27.37827543123572	31.705480674575742	18.968292258996307	21.947951635192233
17	24.89128884392437	35.02420523356823	19.14446522061408	20.940040701893317
18	37.14910806804865	25.272483866163814	17.9043673048989	19.674040760888634
19	24.875906923957135	26.206477783286097	23.63287156990213	25.284743722854643
20	19.94865115757033	25.904430981091807	30.762851435203263	23.3840664261346
21	22.68889682167292	29.163087820595756	26.69699657521968	21.451018782511643
22	20.692842734706986	30.099750273576696	24.718784976508257	24.48862201520806
23	21.844285967060642	28.331136492782385	23.109307562613854	26.715269977543123
24	23.6755261106781	27.578254091971942	24.922837100545596	23.823382696804366
25	21.711156005749007	27.865228791180698	26.508724836399573	23.91489036667072
26	20.379983226864454	25.833590542196283	29.044804225084253	24.74162200585501
27	22.078652911448444	27.276376379185102	27.057203251120026	23.58776745824643
28	22.074054328747224	29.896009677288614	26.138422015831598	21.891513978132572
29	20.212719523867424	29.872627053384086	28.228282998338273	21.68637042441021
30	20.53290741896987	26.906033033306908	30.008566615765847	22.552492931957374
31	21.22627832804886	28.161720462290063	27.870528852599058	22.74147235706202
32	22.44209682900443	29.075981056956955	27.014335107295068	21.46758700674355
33	22.564465894104774	28.30957658744634	28.012383437619835	21.113574080829054
34	21.35028417682252	26.91441336114307	29.476837172760177	22.258465289274234
35	23.102500070147872	27.10100669990117	28.832645884190537	20.96384734576042
36	21.73134300438658	27.775673341626373	27.303422280834667	23.189561373152383
37	21.39058023201798	29.848465008682744	28.52212463873846	20.23883012056081
38	21.48013568157231	29.345115058098024	28.953845818095658	20.220903442234007
39	21.69104694919112	29.085957643156217	28.93295734074095	20.290038066911716
40	20.711237065511874	28.520721681304188	28.8887641815614	21.879277071622536
41	21.277831211486813	29.001123926541034	30.019438784974618	19.701606076997543
42	20.899778956262022	30.61681803018541	28.83514003074036	19.648262982812213
43	22.28239350773653	28.365383116498467	28.38494657849859	20.967276797266415
44	20.6379257397884	29.64321177987252	29.72481726450933	19.99404521582975
45	22.25920146656046	27.815233335567672	28.587017011664827	21.33854818620704
46	21.599243779157135	26.54482759703604	29.68598620399434	22.169942419812482
47	21.02191419512332	28.244728777151124	29.14527156579403	21.58808546193153
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	2.0
6	4.0
7	10.5
8	17.0
9	17.0
10	70.5
11	124.0
12	234.0
13	344.0
14	613.0
15	882.0
16	1234.5
17	1587.0
18	2467.0
19	3347.0
20	4508.0
21	5669.0
22	7422.5
23	9176.0
24	12209.0
25	15242.0
26	15242.0
27	18073.0
28	20904.0
29	25296.5
30	29689.0
31	32955.5
32	36222.0
33	44043.5
34	51865.0
35	56087.5
36	60310.0
37	68260.0
38	76210.0
39	78469.5
40	80729.0
41	89825.5
42	98922.0
43	98922.0
44	106987.0
45	115052.0
46	117661.5
47	120271.0
48	127196.5
49	134122.0
50	132978.0
51	131834.0
52	107670.5
53	83507.0
54	72250.5
55	60994.0
56	55052.0
57	49110.0
58	49110.0
59	41565.0
60	34020.0
61	27801.0
62	21582.0
63	18558.5
64	15535.0
65	13530.5
66	11526.0
67	8481.0
68	5436.0
69	4036.5
70	2637.0
71	2461.0
72	2285.0
73	1980.5
74	1676.0
75	1676.0
76	1217.0
77	758.0
78	668.0
79	578.0
80	694.5
81	811.0
82	418.0
83	25.0
84	13.0
85	1.0
86	1.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.3382623904524068E-4
2	7.79420796817469E-5
3	2.3382623904524068E-4
4	0.0010911891155444566
5	6.235366374539752E-4
6	0.0014808995139531911
7	0.0020264940717254192
8	0.0014808995139531911
9	0.07973474751442708
10	0.00553388765740403
11	9.353049561809627E-4
12	7.014787171357221E-4
13	0.0
14	0.0035073935856786104
15	3.117683187269876E-4
16	3.897103984087345E-4
17	0.0013250153545896974
18	0.03515187793646785
19	0.007482439649447702
20	0.0011691311952262036
21	1.558841593634938E-4
22	0.0
23	0.007872150047856437
24	3.117683187269876E-4
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.009119223322764387
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	1.558841593634938E-4
42	0.0
43	0.0
44	1.558841593634938E-4
45	1.558841593634938E-4
46	0.7250172251996096
47	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
47	1283004.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.198664957114566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.00651687514248	12.096410076006455
2	7.164292545028649	2.750897044535173
3	3.6710014672646443	2.114349816812697
4	2.5157849622399193	1.9319885037676534
5	2.0530219229353035	1.9707640024022988
6	1.7144962740864134	1.9749623721843788
7	1.648823376776646	2.215864530893517
8	1.6025661523181867	2.4613704503975273
9	1.5565771585634278	2.6895783012440977
>10	14.293217954606172	45.513093117026514
>50	0.45675354256374084	6.063950294187194
>100	0.2903971700682217	10.772793395745614
>500	0.01991294880465865	2.4550958643753678
>1k	0.004978237201164662	2.1708383740280626
>5k	0.0012445593002911655	1.4486857640591588
>10k+	4.1485310009705523E-4	1.3693580923343294
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGCACTGAAGAGGAACAGCCGACGATCAATCAGTGCAAGCTTGTTCC	17193	1.3400581759682744	No Hit
TGTACTGAGACTAGGTATCTACAGTCCGACGATCAGTCTAGAAAGGT	6417	0.5001543253177698	No Hit
TGCACTGACCGTCGTACGCTTGCACTGATTTTTTTTTTTTTTTTTTT	6310	0.4918145227918229	No Hit
TGCACTGATTCATAGGCGAAGACTGATCGTCGGACTGTAGAACTCTG	5462	0.42571963922170153	Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCACTGATACGTCTATACGATCAGTCTAAGGAGGGAGCTTGCACTG	4604	0.3588453348547627	No Hit
TGCACTGAAAAAAAGAACGACTCACTATAGTGAGTCGTATTACCGGC	4546	0.3543246942332214	No Hit
TGCACTGATTCATAGGCGTAGAACTCTGAACCCCTATAGTGAGTCGT	2828	0.22042020133998022	No Hit
TGCACTGAAAAAAAGAAAGTGAGTCGTATTACCGGCCGACGATCAGT	2540	0.19797288239163713	No Hit
TGGAATGACGAGGGGCTGCCAGGACTTCCAACAGACGGGCCTCGGGC	2311	0.18012414614451708	No Hit
TGCACTGATGTGTGTGGGAGGAGCTTGCACTGATTTTTTTTTTTTTT	2114	0.16476955644721294	No Hit
TGCACTGATGAAATGAAGTAAGATCAAAGGACATCTGGCGAGTGATG	1959	0.15268853409654218	No Hit
TGCACTGATTCATAGGCGCCTATGAAGACTGATCGTCGGACTGTAGA	1843	0.14364725285345953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	7.79420796817469E-5	0.0	0.0
14	0.0	0.0	7.79420796817469E-5	0.0	0.0
15	0.0	0.0	0.001714725752998432	0.0	0.0
16	0.0	0.0	0.00428681438249608	0.0	0.0
17	0.0	0.0	0.012626616908442998	0.0	0.0
18	0.0	0.0	0.1494149667499088	0.0	0.0
19	0.0	0.0	0.19088015314059817	0.0	0.0
20	0.0	0.0	0.26944576945979903	0.0	0.0
21	0.0	0.0	0.36172919180298735	0.0	0.0
22	0.0	0.0	0.4506611047198606	0.0	0.0
23	0.0	0.0	0.5408400909116418	0.0	0.0
24	0.0	0.0	0.614963008688983	0.0	0.0
25	0.0	0.0	1.1419294094172738	0.0	0.0
26	0.0	7.79420796817469E-4	1.2446570704378161	0.0	0.0
27	0.0	7.79420796817469E-4	1.3526847928767174	0.0	0.0
28	0.0	9.353049561809628E-4	1.495396740773996	0.0	0.0
29	0.0	0.0010911891155444566	1.603814173611306	0.0	0.0
30	0.0	0.0010911891155444566	1.8139460204332956	0.0	0.0
31	0.0	0.0019485519920436724	2.0729475512157407	0.0	0.0
32	0.0	0.003351509426315117	2.2134771208819304	0.0	0.0
33	0.0	0.003351509426315117	2.392198309592176	0.0	0.0
34	0.0	0.003351509426315117	2.612072916374384	0.0	0.0
35	0.0	0.0040529881434508384	2.7572790108214784	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTAGC	25	6.759909E-5	40.97876	23
TTCGACA	20	0.0011154838	40.975563	11
CATAGGC	4400	0.0	40.463367	11
TCATAGG	4415	0.0	40.27949	10
CACTGAA	34050	0.0	40.247746	3
ACTGACG	5680	0.0	40.22435	4
CACTGAC	22345	0.0	40.19266	3
TGACACG	980	0.0	39.972332	6
CACTGAG	30160	0.0	39.957897	3
CTGACCG	2480	0.0	39.943638	5
ATAGGCG	1975	0.0	39.846897	12
CTGATTC	5545	0.0	39.83514	5
TTCATAG	4490	0.0	39.739746	9
TGATTCA	4890	0.0	39.718544	6
ACTGATA	7010	0.0	39.67289	4
CTGACGT	1510	0.0	39.660492	5
ACTGAAG	10405	0.0	39.63895	4
CGTACGC	725	0.0	39.576496	13
ACTGAAT	7595	0.0	39.56052	4
ACTGACC	5820	0.0	39.53867	4
>>END_MODULE