FastQCFastQC Report
Thu 26 May 2016
SRR2099245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31342
Sequences flagged as poor quality0
Sequence length47
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACTGAAGAGGAACAGCCGACGATCAATCAGTGCAAGCTTGTTCC3391.0816157233105737No Hit
TGTACTGAGACTAGGTATCTACAGTCCGACGATCAGTCTAGAAAGGT1460.4658286005998341No Hit
TGCACTGATTCATAGGCGAAGACTGATCGTCGGACTGTAGAACTCTG1390.4434943526258695Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGCACTGATACGTCTATACGATCAGTCTAAGGAGGGAGCTTGCACTG1380.4403037457724459No Hit
TGCACTGACCGTCGTACGCTTGCACTGATTTTTTTTTTTTTTTTTTT1290.4115882840916342No Hit
TGCACTGAAAAAAAGAACGACTCACTATAGTGAGTCGTATTACCGGC1140.3637291812902814No Hit
TGCACTGAATCATTTTAGTTTCAGAACCCAGTTGTGGGACACTGGAC700.22334247973964647No Hit
TGCACTGATGTGTGTGGGAGGAGCTTGCACTGATTTTTTTTTTTTTT630.20100823176568183No Hit
TGCACTGACGTATGAGCGATGCATTGTGCAGGCCCACATGCATACCA620.19781762491225832No Hit
TGCACTGATTCATAGGCGTAGAACTCTGAACCCCTATAGTGAGTCGT600.19143641120541127No Hit
TGCACTGAAAAAAAGAAAGTGAGTCGTATTACCGGCCGACGATCAGT580.18505519749856422No Hit
TGCACTGATGAAATGAAGTAAGATCAAAGGACATCTGGCGAGTGATG540.17229277008487015No Hit
TGGAATGACGAGGGGCTGCCAGGACTTCCAACAGACGGGCCTCGGGC520.1659115563780231No Hit
TGCACTGAAAAAAACCGCATGCCTCATTTCCCCTGTTCCCTGTTCCC470.1499585221109055No Hit
TGCACTGATTCATAGGCGCCTATGAAGACTGATCGTCGGACTGTAGA430.13719609469721142No Hit
TGCACTGACTAATTTGCGTTTTGTTTTTTGTTTTTTTTTTTAATTCT420.13400548784378788No Hit
TGCACTGAGACAGATATGTTCCATCCAGTACTAAATGCTTACCGTGT400.12762427413694086No Hit
TGCACTGAAGAAAAGATGTTTCGGAATGCTCTTTGAAGCAAGAAACA390.12443366728351732No Hit
TGCACTGACACGAGGAGATTTCTGAATGGCCCAGGTCTGAGGCCTCC380.12124306043009381No Hit
TGCACTGAGTATCGGAGATTTAAAGATGCTTCTTAGCACCCATTGTC350.11167123986982323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGCA256.537912E-541.054417
TTTCACT303.9447696E-641.054419
ATTTCAC256.537912E-541.054418
TCTATCC200.00108851241.05439821
TTCTATC200.00108851241.05439820
ATTTGGT200.00108851241.05439818
GCTTTGG200.00108851241.05439817
ATTTCAG200.00108851241.05439818
CTATCCT200.00108851241.05439822
TTCACTG303.9885654E-640.988821
CTGAGTC650.040.988825
CCAATAC256.6000815E-540.9888230
CTGAGCT256.6000815E-540.988825
CTGAGCG458.9858077E-1040.988825
CTGAGAC505.4569682E-1140.988825
ACTGAAT1750.040.988824
GTCGTAC458.9858077E-1040.9888211
TGACACG256.6000815E-540.988826
CTGACTA458.9858077E-1040.988825
CCGTCGT458.9858077E-1040.988829
Produced by FastQC (version 0.11.5)