##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099245_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31342 Sequences flagged as poor quality 0 Sequence length 47 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.58538063939761 41.0 39.0 41.0 34.0 41.0 2 38.04859294237764 40.0 38.0 41.0 33.0 41.0 3 38.19169165975369 40.0 38.0 41.0 33.0 41.0 4 35.68652287665114 37.0 34.0 39.0 30.0 40.0 5 37.732435709271904 39.0 38.0 40.0 34.0 40.0 6 38.27933763001723 40.0 38.0 40.0 34.0 41.0 7 38.148809903643674 40.0 38.0 41.0 34.0 41.0 8 32.86545210899113 34.0 31.0 37.0 25.0 39.0 9 35.788335141343886 37.0 37.0 37.0 35.0 37.0 10 35.74615531874162 37.0 35.0 37.0 35.0 37.0 11 35.651585731606154 37.0 35.0 37.0 35.0 37.0 12 35.491512985769894 37.0 35.0 37.0 33.0 37.0 13 37.161572331057364 39.0 37.0 39.0 34.0 39.0 14 37.076478846276565 39.0 37.0 39.0 34.0 39.0 15 37.146959351668684 39.0 37.0 39.0 34.0 39.0 16 37.20119966817689 39.0 37.0 39.0 34.0 39.0 17 37.05500606215302 39.0 37.0 39.0 33.0 39.0 18 31.362580562823048 31.0 31.0 34.0 30.0 34.0 19 31.134707421351543 31.0 31.0 34.0 28.0 34.0 20 30.627815710548145 31.0 30.0 34.0 27.0 34.0 21 34.06834279880033 35.0 33.0 37.0 30.0 37.0 22 34.62746474379427 35.0 35.0 37.0 32.0 37.0 23 33.94534490460086 35.0 33.0 37.0 29.0 37.0 24 33.69376555420841 35.0 33.0 37.0 28.0 37.0 25 33.926839384851 35.0 33.0 37.0 29.0 37.0 26 35.64172675642907 37.0 34.0 39.0 30.0 39.0 27 36.14833131261566 37.0 35.0 39.0 32.0 39.0 28 36.56282304894391 38.0 35.0 39.0 32.0 39.0 29 36.83322697977155 38.0 35.0 39.0 33.0 39.0 30 36.93558164762938 38.0 37.0 39.0 33.0 39.0 31 38.22876651139047 40.0 37.0 40.0 34.0 41.0 32 38.484238402144086 40.0 38.0 40.0 35.0 41.0 33 38.657839321038864 40.0 38.0 40.0 36.0 41.0 34 38.807606406738564 40.0 38.0 40.0 36.0 41.0 35 38.88166039180652 40.0 38.0 40.0 36.0 41.0 36 38.977665752026034 40.0 38.0 40.0 36.0 41.0 37 38.93350775317465 40.0 38.0 40.0 36.0 41.0 38 38.85919851955842 40.0 38.0 40.0 36.0 41.0 39 38.72831982643099 40.0 38.0 40.0 35.0 41.0 40 38.60075936443111 40.0 38.0 40.0 35.0 41.0 41 38.43564545976645 40.0 38.0 40.0 35.0 41.0 42 38.209463339927254 40.0 38.0 40.0 34.0 41.0 43 38.13221874800587 40.0 37.0 40.0 34.0 41.0 44 38.04198838619105 40.0 37.0 40.0 34.0 41.0 45 37.938548912003064 40.0 37.0 40.0 34.0 41.0 46 37.92993427349882 40.0 37.0 40.0 34.0 41.0 47 37.90833386510114 40.0 37.0 40.0 34.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9942528210382022 1101 2 1.0702803015736322 1101 3 0.580755503521516 1101 4 -0.33128374710597797 1101 5 0.2799745993957785 1101 6 1.1154457148439576 1101 7 0.2868416669735723 1101 8 1.2697594119199493 1101 9 0.5923411666939273 1101 10 0.6756073803087261 1101 11 0.9284799237219445 1101 12 0.7395831516092457 1101 13 0.7932464953844587 1101 14 1.21063619620179 1101 15 1.1797545627742068 1101 16 0.6927677530323706 1101 17 1.4826781206686803 1101 18 0.12712129686525842 1101 19 -0.21267005760442714 1101 20 0.010085096205500577 1101 21 0.01879904291319434 1101 22 0.06487708135200876 1101 23 0.5602367576930192 1101 24 0.20324262724746944 1101 25 0.4182523321775591 1101 26 0.15067458491603247 1101 27 -0.003614547632970755 1101 28 -0.15710602146978658 1101 29 0.2658604565042779 1101 30 0.5746987275436979 1101 31 0.983957499681722 1101 32 0.27633990537920994 1101 33 0.7017931838689648 1101 34 0.8153004117422284 1101 35 0.6828486311538882 1101 36 0.48731271403371323 1101 37 0.9041752159000609 1101 38 0.4326500265339419 1101 39 0.08957673297309299 1101 40 0.3934167348567996 1101 41 0.01784707119519169 1101 42 1.3524540238741238 1101 43 0.5788738719002993 1101 44 0.5861103963500938 1101 45 0.3363432257083758 1101 46 0.6114835430061021 1101 47 0.6251258194298046 1102 1 0.29754952433490445 1102 2 0.013137444430775247 1102 3 0.25108517385118745 1102 4 0.6276906118683812 1102 5 0.6587291781503595 1102 6 0.769658169056413 1102 7 0.3967317768636818 1102 8 1.220675162835704 1102 9 0.5286049029576603 1102 10 0.7854974901988356 1102 11 0.22591582115784092 1102 12 0.3798761919022837 1102 13 0.27749557963353766 1102 14 -0.4311220455564566 1102 15 0.5006336836533265 1102 16 0.040753101017720894 1102 17 1.3046561426466994 1102 18 0.09708466682862849 1102 19 0.17487572994135903 1102 20 0.6093524954728977 1102 21 0.24077706489121198 1102 22 -0.6552694387945124 1102 23 0.9602367576930178 1102 24 -0.07294784894300932 1102 25 -0.8645315506063298 1102 26 0.875216709458158 1102 27 -0.787497331515759 1102 28 -1.1439192082829734 1102 29 -0.17882818818436874 1102 30 0.1856877385327067 1102 31 -0.347178031453808 1102 32 0.3510651801044844 1102 33 -0.7524192703434949 1102 34 -0.9158351193933001 1102 35 -1.1962722479669878 1102 36 -1.1295371028161014 1102 37 -0.6254951137702633 1102 38 -0.471379277495366 1102 39 -0.2547456113492501 1102 40 -0.7347883933483317 1102 41 0.12480677815489827 1102 42 0.44769211911221873 1102 43 -0.7420052489788205 1102 44 -0.7999701897304874 1102 45 0.1619842513494021 1102 46 0.23419416571672258 1102 47 -0.35875696445297933 1103 1 0.42813986422756756 1103 2 0.5015095374540337 1103 3 -0.4591116061130336 1103 4 0.26871625289402346 1103 5 0.8042270910901337 1103 6 1.0928543527177084 1103 7 0.6722237267742344 1103 8 1.6458391461392168 1103 9 0.49998236270721463 1103 10 0.5261057191791565 1103 11 0.3749915516289164 1103 12 0.7382542479547567 1103 13 1.2763029737897682 1103 14 0.8372142692914863 1103 15 0.4594887820433087 1103 16 -0.30390998783141043 1103 17 1.020219648907883 1103 18 0.18891531679881268 1103 19 0.5833432314321172 1103 20 -0.41449962140247365 1103 21 0.4447126641756469 1103 22 0.27019269596994633 1103 23 -0.1560423120744261 1103 24 -0.1814749807259517 1103 25 0.6029699401509774 1103 26 -0.1629466775424362 1103 27 0.6442259839284858 1103 28 -0.2262090115030091 1103 29 0.18014617078998896 1103 30 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1104 19 0.2682823233479539 1104 20 -0.004200618080215435 1104 21 -0.06691524280109462 1104 22 0.19821041468534162 1104 23 0.05547485293111265 1104 24 -0.4062811822763379 1104 25 -1.4341286202033956 1104 26 -0.9398016055601559 1104 27 -0.12266216668059116 1104 28 -0.45710602146979085 1104 29 0.01347950412332466 1104 30 0.5318415846865534 1104 31 -0.2208044050801803 1104 32 0.4572922863315867 1104 33 0.17798366005943933 1104 34 0.09149088793270721 1104 35 -0.22191327360801694 1104 36 0.2587412854622855 1104 37 0.41846093018578046 1104 38 0.5469357408196558 1104 39 0.3991005424969032 1104 40 0.6791310205710843 1104 41 0.5845137378618617 1104 42 0.16197783339793403 1104 43 1.0931595861860188 1104 44 0.09563420587390681 1104 45 1.1125337018988475 1104 46 0.2543406858632409 1104 47 0.32988772419171397 1105 1 -1.6438424170570372 1105 2 -1.7249577936644656 1105 3 -1.4430540202880096 1105 4 -0.5646170804393122 1105 5 -0.18193016250898353 1105 6 -0.9226495232512804 1105 7 -0.9036345235026175 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44 0.1908723011120017 1105 45 0.3506289399940883 1105 46 -0.15042121889866422 1105 47 -0.19392179961781153 1106 1 -1.2695345543770813 1106 2 0.3154630258261264 1106 3 -0.13353021076419935 1106 4 -0.033609328501327695 1106 5 -0.5911217461191711 1106 6 -0.4187735542590332 1106 7 0.09082837793702225 1106 8 -0.05183527246543207 1106 9 0.1744009673583804 1106 10 -0.0785454436115387 1106 11 0.14243341209403582 1106 12 -0.09895505437082619 1106 13 -0.18881330528000007 1106 14 -0.5581345679178114 1106 15 -0.8660926133055327 1106 16 -0.024840220389556578 1106 17 -0.8635012813246803 1106 18 -0.7413172413407239 1106 19 -1.1375870011260218 1106 20 -0.9493833423327054 1106 21 -0.7180780334987702 1106 22 -1.1019003272858683 1106 23 0.37884140885580564 1106 24 -0.22798660863293208 1106 25 -0.2574951761280886 1106 26 -0.41876063103080696 1106 27 0.06283063509128084 1106 28 -0.06341831382859198 1106 29 -0.4710166199076866 1106 30 -0.1542049269413539 1106 31 0.45306048971494306 1106 32 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2213 4 -0.08977431314371387 2213 5 0.09075627055480595 2213 6 0.21571525662292856 2213 7 0.216760804439879 2213 8 -0.5437176770288374 2213 9 -0.6658798575648319 2213 10 0.24703595173729553 2213 11 0.4416874708917575 2213 12 -0.045861592326069456 2213 13 0.3684486517186869 2213 14 0.25538013151176386 2213 15 0.31775995360978726 2213 16 -0.20211095316708594 2213 17 -0.22945125938522892 2213 18 0.07483019174396333 2213 19 0.06118438983492425 2213 20 -0.19422757225811083 2213 21 0.65006588927438 2213 22 0.46325983067815457 2213 23 0.3564631727873575 2213 24 0.732083597597871 2213 25 0.0479019278648849 2213 26 -1.0822095121190074 2213 27 -1.1464716904901167 2213 28 -0.41532704572854584 2213 29 -0.4424953386439725 2213 30 0.29599252808277754 2213 31 0.24595210884614005 2213 32 0.2596283150827148 2213 33 -0.4248914522496321 2213 34 -0.22836535645183886 2213 35 -0.45461767612373905 2213 36 -0.4420673398746402 2213 37 0.027625350670952287 2213 38 0.28817563300294324 2213 39 0.5909244418679762 2213 40 0.0349261688190623 2213 41 0.12674194990139398 2213 42 -0.04916322679972751 2213 43 -0.23837680195414634 2213 44 -0.9847790915205223 2213 45 -0.3164869629708704 2213 46 -0.4774652440558924 2213 47 -0.9818822668235612 2214 1 -0.2937023610346259 2214 2 -0.5947056928241281 2214 3 -0.408040014685767 2214 4 -0.9273621784785249 2214 5 -0.8163839440215881 2214 6 -0.844218150702261 2214 7 -0.004474859637070949 2214 8 -1.37057672253383 2214 9 0.22091259526535367 2214 10 -0.20394444041956916 2214 11 0.19122502176115574 2214 12 0.05106774544677961 2214 13 -0.2941484625987414 2214 14 -0.2621929354508765 2214 15 -0.015203420419069857 2214 16 -0.1839829472477419 2214 17 -0.31676165524168454 2214 18 -0.1412260420703184 2214 19 -0.144882942758489 2214 20 -0.27450874132951597 2214 21 -0.4002485761344303 2214 22 0.4503112550214752 2214 23 -0.07897892858149191 2214 24 0.20156195497855833 2214 25 -0.015361113200597742 2214 26 0.5629995148880269 2214 27 0.128038113431451 2214 28 0.040092858082033445 2214 29 0.27818538647626667 2214 30 0.031841584686553404 2214 31 0.40244489463970723 2214 32 -0.7303827836964203 2214 33 0.07714332392498591 2214 34 0.1867289831708021 2214 35 -0.1434819010589976 2214 36 0.18871327425780748 2214 37 0.5193012663202339 2214 38 0.5497368612678315 2214 39 0.8794926993596519 2214 40 0.37380889171954124 2214 41 0.5410963709150778 2214 42 0.40847643283770907 2214 43 0.7346161688190662 2214 44 0.5620207604957557 2214 45 0.41085302962994064 2214 46 0.8887944673758454 2214 47 0.6744255393177596 2215 1 -0.17823395144856846 2215 2 0.43906337035669907 2215 3 0.7738771966432054 2215 4 0.23167921585698537 2215 5 -0.07346455404337604 2215 6 -0.6448717454735018 2215 7 -0.12321124307933928 2215 8 -0.5699231277625927 2215 9 -0.14945777510502012 2215 10 0.2285174332187765 2215 11 -0.06040896516694261 2215 12 0.24169955372564544 2215 13 0.4128232149611719 2215 14 0.6757155612811516 2215 15 0.271288954308595 2215 16 -0.053263992999369236 2215 17 -0.6511843131937525 2215 18 0.010190079934041307 2215 19 -0.18409862903299867 2215 20 0.2418311279515315 2215 21 0.5997514238655697 2215 22 0.7564114728863984 2215 23 0.9972737947300558 2215 24 0.2974225214273645 2215 25 -0.07963126570604118 2215 26 -0.38689155265010555 2215 27 0.14982460580618095 2215 28 0.13813207376830405 2215 29 -0.5420760514322325 2215 30 -0.44963989679492755 2215 31 0.2553860711102942 2215 32 0.31972614876545435 2215 33 -0.07863009655432052 2215 34 0.3533956498374664 2215 35 -0.1663577180524598 2215 36 -0.11956559284459445 2215 37 -0.2508512391263906 2215 38 0.09984579372970614 2215 39 0.6028042462006127 2215 40 0.4674908089308758 2215 41 0.07922273257085521 2215 42 0.41065508207518064 2215 43 0.8021542951807277 2215 44 0.7570098672495646 2215 45 0.5807876701528158 2215 46 0.46068989221245005 2215 47 -0.23624984194585608 2216 1 -0.38193765515227796 2216 2 -0.40352922223588905 2216 3 -1.0710302107641994 2216 4 0.47704958622735916 2216 5 0.20199840891958587 2216 6 -0.40181618991795176 2216 7 0.5666035717354774 2216 8 0.10368798334852158 2216 9 -0.6540874047346463 2216 10 -0.39879738159604017 2216 11 0.16181325705527883 2216 12 -0.22589303886694978 2216 13 -0.9228249331869733 2216 14 -0.6090066609410698 2216 15 0.2041593246789688 2216 16 -0.4560417707771478 2216 17 -0.2530361650456072 2216 18 0.08426415400811393 2216 19 0.19090137096700133 2216 20 0.8946089057293101 2216 21 0.6414180905322411 2216 22 0.4184485099234365 2216 23 0.7310700910263535 2216 24 0.6562188177236621 2216 25 0.5152761417013636 2216 26 1.0691269658684135 2216 27 1.0826949761765476 2216 28 0.38813207376830405 2216 29 0.11764617078998896 2216 30 0.6568415846865534 2216 31 -0.11961392888970579 2216 32 -0.1617553327160266 2216 33 -0.12261157803580147 2216 34 -7.710168291978903E-4 2216 35 0.13225339305865447 2216 36 0.22302699974800078 2216 37 -0.19761049838565015 2216 38 -0.3816356877517748 2216 39 0.3157672091635746 2216 40 0.6850834015234639 2216 41 0.28987088071900047 2216 42 1.0101921191122187 2216 43 0.3878024433288729 2216 44 0.8009913487310456 2216 45 0.6155098923750373 2216 46 0.7037454477680072 2216 47 0.844768676572663 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 1.0 25 2.0 26 6.0 27 30.0 28 107.0 29 218.0 30 334.0 31 554.0 32 799.0 33 1069.0 34 1676.0 35 3035.0 36 6119.0 37 9120.0 38 7088.0 39 1184.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.6221683364175866 0.4977346691340693 98.62803905302788 0.2520579414204582 2 98.04090488497495 0.30949873967008074 1.2220414153983599 0.42755495995660636 3 1.3368642715844554 0.153149128964329 0.8199859613298449 97.69000063812136 4 0.26162976198072874 98.64080148044158 0.3318231127560462 0.7657456448216451 5 0.14676791525748198 0.4115882840916342 0.14038670155063493 99.30125709910025 6 0.3573479675834344 0.1627209495245996 99.35230680875502 0.12762427413694086 7 98.81947546423329 0.43073192521217535 0.5934528747367749 0.15633973581775254 8 1.9720467164464868 96.61752504946072 0.7754164273406089 0.6350118067521859 9 27.989789406509253 28.158902361199743 23.790682833439693 20.060625398851307 10 28.652563734405412 28.7865734979739 24.134520276953513 18.426342490667178 11 20.793848313710477 35.4551545898344 19.760058709039278 23.990938387415845 12 27.429647118882013 35.15729691787378 20.62089209367622 16.792163869567993 13 26.673473294620635 32.98130304383894 20.80275668432136 19.542466977219068 14 24.660200370110395 36.10809776019399 20.965477633845957 18.26622423584966 15 29.63116584774424 31.855018824580434 20.761278795226854 17.75253653244847 16 27.126539467806776 31.1722289579478 19.749856422691593 21.951375151553826 17 25.384468125837532 34.36921702507817 19.325505711186267 20.92080913789803 18 40.44549400051059 24.498978810314014 15.496553484809802 19.558973704365588 19 17.612149830897835 24.20075298321741 38.79458873077659 19.392508455108164 20 13.649416118945823 16.071086720694275 55.50379682215557 14.775700338204325 21 19.520132729245102 20.4422181098845 44.2760513049582 15.761597855912193 22 15.200051049709653 20.51241146065982 18.846914683172738 45.44062280645779 23 25.355888924353653 30.87775295244175 19.13501436323013 24.631343759974467 24 24.254898206650076 26.69921501053035 26.83004658880592 22.21584019401366 25 21.48554655095399 26.810669389317848 28.342160678961136 23.36162338076702 26 22.165145810733204 25.132410184417076 28.792036245293858 23.91040775955587 27 21.772701167762108 26.781953927637037 28.54316891072682 22.902175993874035 28 22.423584965860506 28.511262842192586 28.859038989215748 20.20611320273116 29 19.49779848127114 27.33711952013273 31.200944419628613 21.96413757896752 30 20.040201646353136 26.08959224044413 31.06055771807798 22.80964839512475 31 21.523833833195074 26.963818518282178 29.261055452747115 22.251292195775637 32 22.458681641248166 27.58598685469976 29.679024950545596 20.276306553506476 33 21.5812647565567 27.2382107076766 30.69044732308085 20.490077212685854 34 22.066236998277073 25.51847361368132 30.90740858911365 21.507880798927957 35 22.975559951502774 27.49984046965733 29.73964648076064 19.784953098079257 36 21.239869823240383 26.976580945695872 29.535447642141538 22.24810158892221 37 20.592176631995404 28.938804160551335 30.208665688213898 20.26035351923936 38 22.251292195775637 28.1475336609023 30.760640673856166 18.840533469465893 39 21.415353200178675 28.823942313828088 29.64711888201136 20.113585603981875 40 21.21115436155957 27.921000574309236 30.693637929934276 20.174207134196926 41 21.397574984045946 28.337587747287813 31.66560306317805 18.599234205488195 42 20.4964584263927 30.744687639589053 29.49396975304703 19.26488418097122 43 21.290919532895156 28.24325186650501 30.773403101269864 19.69242549932997 44 20.676451818761965 29.40331844288449 31.26037013401404 18.659859604339502 45 21.81557115507339 28.688576898532226 29.307594128908743 20.188257817485642 46 21.115436155956864 26.746857252249377 30.349052389764537 21.788654202029225 47 21.083530087422627 27.972050283964013 30.786165528683558 20.158254099929806 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 2.0 7 3.0 8 4.0 9 4.0 10 11.5 11 19.0 12 33.0 13 47.0 14 65.5 15 84.0 16 95.0 17 106.0 18 119.0 19 132.0 20 167.5 21 203.0 22 271.0 23 339.0 24 402.5 25 466.0 26 466.0 27 548.0 28 630.0 29 727.5 30 825.0 31 879.5 32 934.0 33 1120.5 34 1307.0 35 1429.0 36 1551.0 37 1740.5 38 1930.0 39 1908.0 40 1886.0 41 2197.5 42 2509.0 43 2509.0 44 2653.0 45 2797.0 46 2780.0 47 2763.0 48 2971.5 49 3180.0 50 3017.5 51 2855.0 52 2481.0 53 2107.0 54 1759.0 55 1411.0 56 1251.5 57 1092.0 58 1092.0 59 918.0 60 744.0 61 627.5 62 511.0 63 451.0 64 391.0 65 321.5 66 252.0 67 174.0 68 96.0 69 70.0 70 44.0 71 51.0 72 58.0 73 43.5 74 29.0 75 29.0 76 21.0 77 13.0 78 11.5 79 10.0 80 12.5 81 15.0 82 7.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0031906068534235214 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.012762427413694086 9 0.006381213706847043 10 0.0031906068534235214 11 0.0031906068534235214 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.019143641120541125 19 0.0 20 0.0 21 0.0 22 0.0 23 0.03828728224108225 24 0.012762427413694086 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.006381213706847043 42 0.0 43 0.0 44 0.006381213706847043 45 0.006381213706847043 46 0.0 47 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 47 31342.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.23916788973263 #Duplication Level Percentage of deduplicated Percentage of total 1 79.40739221817809 49.422500159530344 2 11.298508227815656 14.06419500989088 3 3.5320654124160558 6.594984366026418 4 1.7993540780232737 4.479612022206624 5 1.0252729789306403 3.1906068534235215 6 0.7433229097247143 2.775827962478463 7 0.49725739478136055 2.166422053474571 8 0.3690982724150305 1.8377895475719483 9 0.28707643410057926 1.6080658541254547 >10 0.9740093299841083 9.316572011996682 >50 0.03588455426257241 1.3368642715844554 >100 0.030758189367919207 3.2065598876906387 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGCACTGAAGAGGAACAGCCGACGATCAATCAGTGCAAGCTTGTTCC 339 1.0816157233105737 No Hit TGTACTGAGACTAGGTATCTACAGTCCGACGATCAGTCTAGAAAGGT 146 0.4658286005998341 No Hit TGCACTGATTCATAGGCGAAGACTGATCGTCGGACTGTAGAACTCTG 139 0.4434943526258695 Illumina DpnII expression Sequencing Primer (100% over 23bp) TGCACTGATACGTCTATACGATCAGTCTAAGGAGGGAGCTTGCACTG 138 0.4403037457724459 No Hit TGCACTGACCGTCGTACGCTTGCACTGATTTTTTTTTTTTTTTTTTT 129 0.4115882840916342 No Hit TGCACTGAAAAAAAGAACGACTCACTATAGTGAGTCGTATTACCGGC 114 0.3637291812902814 No Hit TGCACTGAATCATTTTAGTTTCAGAACCCAGTTGTGGGACACTGGAC 70 0.22334247973964647 No Hit TGCACTGATGTGTGTGGGAGGAGCTTGCACTGATTTTTTTTTTTTTT 63 0.20100823176568183 No Hit TGCACTGACGTATGAGCGATGCATTGTGCAGGCCCACATGCATACCA 62 0.19781762491225832 No Hit TGCACTGATTCATAGGCGTAGAACTCTGAACCCCTATAGTGAGTCGT 60 0.19143641120541127 No Hit TGCACTGAAAAAAAGAAAGTGAGTCGTATTACCGGCCGACGATCAGT 58 0.18505519749856422 No Hit TGCACTGATGAAATGAAGTAAGATCAAAGGACATCTGGCGAGTGATG 54 0.17229277008487015 No Hit TGGAATGACGAGGGGCTGCCAGGACTTCCAACAGACGGGCCTCGGGC 52 0.1659115563780231 No Hit TGCACTGAAAAAAACCGCATGCCTCATTTCCCCTGTTCCCTGTTCCC 47 0.1499585221109055 No Hit TGCACTGATTCATAGGCGCCTATGAAGACTGATCGTCGGACTGTAGA 43 0.13719609469721142 No Hit TGCACTGACTAATTTGCGTTTTGTTTTTTGTTTTTTTTTTTAATTCT 42 0.13400548784378788 No Hit TGCACTGAGACAGATATGTTCCATCCAGTACTAAATGCTTACCGTGT 40 0.12762427413694086 No Hit TGCACTGAAGAAAAGATGTTTCGGAATGCTCTTTGAAGCAAGAAACA 39 0.12443366728351732 No Hit TGCACTGACACGAGGAGATTTCTGAATGGCCCAGGTCTGAGGCCTCC 38 0.12124306043009381 No Hit TGCACTGAGTATCGGAGATTTAAAGATGCTTCTTAGCACCCATTGTC 35 0.11167123986982323 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.006381213706847042 0.0 0.0 18 0.0 0.0 0.09252759874928211 0.0 0.0 19 0.0 0.0 0.12762427413694086 0.0 0.0 20 0.0 0.0 0.1691021632314466 0.0 0.0 21 0.0 0.0 0.2233424797396465 0.0 0.0 22 0.0 0.0 0.2520579414204582 0.0 0.0 23 0.0 0.0 0.2839640099546934 0.0 0.0 24 0.0 0.0 0.3477761470231638 0.0 0.0 25 0.0 0.0 0.8359389955969625 0.0 0.0 26 0.0 0.0 0.90613234637228 0.0 0.0 27 0.0 0.0 1.0273754068023737 0.0 0.0 28 0.0 0.0 1.1294748261119265 0.0 0.0 29 0.0 0.0 1.2251930317146322 0.0 0.0 30 0.0 0.0 1.346436092144726 0.0 0.0 31 0.0 0.0 1.5314912896432902 0.0 0.0 32 0.0 0.0 1.6144470678323017 0.0 0.0 33 0.0 0.0 1.732499521408972 0.0 0.0 34 0.0 0.0 1.8377895475719481 0.0 0.0 35 0.0 0.0 1.911173505200689 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTTGCA 25 6.537912E-5 41.0544 17 TTTCACT 30 3.9447696E-6 41.0544 19 ATTTCAC 25 6.537912E-5 41.0544 18 TCTATCC 20 0.001088512 41.054398 21 TTCTATC 20 0.001088512 41.054398 20 ATTTGGT 20 0.001088512 41.054398 18 GCTTTGG 20 0.001088512 41.054398 17 ATTTCAG 20 0.001088512 41.054398 18 CTATCCT 20 0.001088512 41.054398 22 TTCACTG 30 3.9885654E-6 40.98882 1 CTGAGTC 65 0.0 40.98882 5 CCAATAC 25 6.6000815E-5 40.98882 30 CTGAGCT 25 6.6000815E-5 40.98882 5 CTGAGCG 45 8.9858077E-10 40.98882 5 CTGAGAC 50 5.4569682E-11 40.98882 5 ACTGAAT 175 0.0 40.98882 4 GTCGTAC 45 8.9858077E-10 40.98882 11 TGACACG 25 6.6000815E-5 40.98882 6 CTGACTA 45 8.9858077E-10 40.98882 5 CCGTCGT 45 8.9858077E-10 40.98882 9 >>END_MODULE