FastQCFastQC Report
Thu 26 May 2016
SRR2099244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186079
Sequences flagged as poor quality0
Sequence length47
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGAACGTCTGAGTCCGACGATCGTCGGACTGTAGAACTCTG127750.5843796129966026Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTATAGTGATTATCTGAGTAGAACTCTGAACCCCTATAGTGAGTCGT127370.5826413409579434No Hit
CTATAGTGATTATCTGAGATCGTCGGACTGTAGAACTCTGAACCCCT58410.2671907099423214Illumina DpnII expression Sequencing Primer (96% over 27bp)
CTATAGTGCAACGATGAGTAGAACTCTGAACCCCTATAGTGAGTCGT50870.23269973317524206No Hit
CTATAGTGCAACGATGAGTGACGATCGTCGGACTGTAGAACTCTGAA46660.21344150874693918Illumina DpnII expression Sequencing Primer (100% over 25bp)
CTATAGTGATTATCTGAGTCGGACTGTAGAACTCTGAACCCCTATAG45610.20863838864011774Illumina NlaIII expression Adapter 1 (95% over 24bp)
CTATAGTGAACGTCTGAGTAGAACTCTGAACCCCTATAGTGAGTCGT45040.20603098058212901No Hit
CTAGACTGTAGATTCTCTCCGACGATCGTCAAACGCGGATGTACCTA39470.18055157201546695No Hit
CTAGACTGTACTGGCACTTGGACCTCTTTGAGGCTTTCGTTGGAAAC34480.15772531550781102No Hit
CTATAGTGATTATCTGAGATAATAGCTGATCGTCGGACTGTAGAACT29410.1345331069920163No Hit
CTAGACGTCGGGCGCCACTGCAAAGCCCAGCACCACCCAGGGTGTGG23550.1077271223958512No Hit
CTAGACTGATGTGGCATATCGTCAGGGGAAAAGGTACCTAGACTGTT22370.1023293302758043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTGC416050.040.720993
GACTGCG122050.040.5532464
GATTATC102550.040.486068
AGACTGG475300.040.4503443
GTGATTA103100.040.370446
TGATTAT104500.040.339727
TAGTGAT103750.040.2953764
AGACTGA535550.040.2908973
ACTGCGC28300.040.064975
ACTGCGG38200.039.987025
GACTGCC90550.039.9652374
ATTATCT103700.039.8789149
GACTGGC109300.039.8625344
GACTGAC123250.039.8589634
AGTGATT104950.039.8541765
TAGTGCA55000.039.8511734
GCAACGA54700.039.806448
GACTGTC98800.039.803184
GACTGGG157500.039.7961434
GACTGCA119250.039.7001044
Produced by FastQC (version 0.11.5)