FastQCFastQC Report
Thu 26 May 2016
SRR2099243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34367
Sequences flagged as poor quality0
Sequence length47
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGATTATCTGAGTAGAACTCTGAACCCCTATAGTGAGTCGT2540.7390810952367096No Hit
CTATAGTGAACGTCTGAGTCCGACGATCGTCGGACTGTAGAACTCTG1600.4655628946372974Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTATAGTGCAACGATGAGTAGAACTCTGAACCCCTATAGTGAGTCGT1060.30843541769720956No Hit
CTAGACTGTAGATTCTCTCCGACGATCGTCAAACGCGGATGTACCTA1010.293886577239794No Hit
CTATAGTGATTATCTGAGTCGGACTGTAGAACTCTGAACCCCTATAG950.27642796869089536Illumina NlaIII expression Adapter 1 (95% over 24bp)
CTATAGTGAACGTCTGAGTAGAACTCTGAACCCCTATAGTGAGTCGT930.27060843250792915No Hit
CTAGACTGTACTGGCACTTGGACCTCTTTGAGGCTTTCGTTGGAAAC830.24151075159309804No Hit
CTATAGTGATTATCTGAGATCGTCGGACTGTAGAACTCTGAACCCCT750.21823260686123316Illumina DpnII expression Sequencing Primer (96% over 27bp)
CTAGACTGAGGGGCTCGGCTTCGCCCAGCCTGTACTTGGGAGGGAGG750.21823260686123316No Hit
CTAGACTGGGTACTAACGGATCGACCGACACTAGGGGAATTTCGACC730.21241307067826695No Hit
CTAGACTGATGTGGCATATCGTCAGGGGAAAAGGTACCTAGACTGTT590.17167631739750341No Hit
CTAGACGTCGGGCGCCACTGCAAAGCCCAGCACCACCCAGGGTGTGG550.160037245031571No Hit
CTAGACTGCAGGATGAAGTTTGGGTGTGGGTTTCTGGTTTAAATCAA480.13966886839118922No Hit
CTATAGTGCAACGATGAGTGACGATCGTCGGACTGTAGAACTCTGAA480.13966886839118922Illumina DpnII expression Sequencing Primer (100% over 25bp)
CTAGACTGAAGCATTCTATTTCCTCTCTGCTTTAAGTCTATCATACT470.13675910029970612No Hit
CTATAGTGATTATCTGAGGACTGTAGAACTCTGAACCCCTATAGTGA380.11057118747635813Illumina DpnII expression Adapter 1 (95% over 21bp)
CTATAGTGATTATCTGAATCGTCGGACTGTAGAACTCTGAACCCCTA370.10766141938487503Illumina DpnII expression Sequencing Primer (96% over 27bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGT553.6379788E-1241.0000045
ACTGTGG553.6379788E-1241.0000045
ACTGCCA553.6379788E-1241.0000045
GTGCAAC750.041.0000046
ACTGAAC553.6379788E-1241.0000045
ATAGTGC750.041.0000043
TATCTGA1350.041.00000411
ACTGTTG505.4569682E-1141.05
ACTGTGA401.4768375E-841.05
ACTGCCT303.992547E-641.05
ACTGTCG459.0221874E-1041.05
ACTGCAT505.4569682E-1141.05
ACTGTCC401.4768375E-841.05
ACTGCAG401.4768375E-841.05
AACGCGG200.001097162141.032
CTGAGCG200.001097162141.06
TCGATCG200.001097162141.017
ACTGAGT303.992547E-641.05
ACTGACG459.0221874E-1041.05
ACTGAAT256.603006E-541.05
Produced by FastQC (version 0.11.5)