FastQCFastQC Report
Thu 26 May 2016
SRR2099241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22416
Sequences flagged as poor quality0
Sequence length47
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACGTACGCGCCCTACTACGTTTTTTTTTTTTTTTTTTTTTTTT3611.6104568165596003No Hit
GGCTTCGTCCATGGCCTGGTCACAGCGGGCTCGGGCGTGGCCATCGG1990.8877587437544611No Hit
GACTACGTTATCGCACCCTTGGCTCCTCGAGCCAGCGTCGAGTTCTT1310.5844039971448965No Hit
GACTACGTCGGAGTCCCCGACTCACTATAGTGAGTCGTATTACCGGC970.43272662384011423No Hit
GACTACGTATATAGAATGTTTAGGACAGCTCAACTTATCACCAGATC510.22751605995717344No Hit
GACTACGTGCAGAAGGGTGATGATCGGAGTGTCGTCACCCGGGAAGT510.22751605995717344No Hit
GACTACGTGACCACTGAGCCAACGTCACATTAGGGTCCGCAAGCAGG430.19182726623840116No Hit
GACTACGTCGATTCGTCCTTTCATCCAAACTTCCCAGAAGCGAATCT400.17844396859386152No Hit
GACTACGTCACCAATCAGCTTCACCACAGCTCAGAAGAGGGTGTACA370.16506067094932192No Hit
GACTACGTGGTCAATAAACGATCTCGACTGCACAGTCAGTGACTACG360.16059957173447537No Hit
GACTACGTCACCTGCGTATCTCTATACACGTGGCAGTGACTACGTTT330.14721627408993576No Hit
GACTACGTGTATGTGGGGTTTCCTTGGTAGGTTTTGTTTCTTTTGTA320.14275517487508924No Hit
GACTACGTTGAGGCCCTTCCGACGATCTCGAAGATGAGGCCAGTGAC320.14275517487508924No Hit
GACTACGTTTAGTTTTAGAAGAAATGTAACACTGTGTAAGAAAACAC280.12491077801570308No Hit
GACTACGTGAAACCGTAATTTCGAAGAGATGAGGAAGGCTGGTATCT240.10706638115631692No Hit
GACTACGTACGGCGAACACGCAAGCAGGCTGAATGGGATGCTCAGAA230.10260528194147038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGT352.3737448E-741.0000045
ACGTTCC352.3737448E-741.0000045
ACGTTCA352.3737448E-741.0000045
ACGTTAT352.3737448E-741.0000045
ACGTTAA352.3737448E-741.0000045
TACGTTA950.041.0000044
TACGTAC1400.041.0000044
ACGTGAA352.3737448E-741.0000045
GTCGGAG352.3737448E-741.0000047
GCCCTAC200.001088968641.013
ACAGCGG200.001088968641.022
CGTTCCT200.001088968641.06
CGTTCAC200.001088968641.06
TGGTCAC200.001088968641.017
CCTACTA200.001088968641.015
CGGAGTC303.930156E-641.09
CGTTATC303.930156E-641.06
ACGTCTT401.4375473E-841.05
ACGTCTG256.5291686E-541.05
ACGTCTA303.930156E-641.05
Produced by FastQC (version 0.11.5)