##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099241_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22416 Sequences flagged as poor quality 0 Sequence length 47 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.538142398286936 37.0 33.0 38.0 27.0 39.0 2 31.971672019985725 34.0 29.0 37.0 19.0 39.0 3 36.080121341898646 37.0 34.0 39.0 31.0 40.0 4 36.40132048536759 39.0 37.0 39.0 31.0 40.0 5 35.676258029978584 38.0 35.0 39.0 30.0 40.0 6 36.89034618129907 38.0 37.0 39.0 33.0 40.0 7 36.96672019985724 39.0 37.0 40.0 32.0 40.0 8 34.20712883654532 37.0 32.0 39.0 25.0 39.0 9 35.48211099214846 37.0 35.0 37.0 33.0 37.0 10 35.25713775874375 37.0 35.0 37.0 32.0 37.0 11 35.02609743040685 37.0 35.0 37.0 32.0 37.0 12 35.13829407566024 37.0 35.0 37.0 32.0 37.0 13 36.66251784439686 39.0 37.0 39.0 32.0 39.0 14 36.652257316202714 39.0 37.0 39.0 32.0 39.0 15 36.67175231977159 39.0 37.0 39.0 32.0 39.0 16 36.649848322626696 39.0 37.0 39.0 32.0 39.0 17 36.42643647394718 39.0 37.0 39.0 32.0 39.0 18 31.08377944325482 31.0 30.0 31.0 30.0 34.0 19 30.60264989293362 31.0 30.0 33.0 27.0 34.0 20 30.034707351891505 31.0 30.0 33.0 26.0 34.0 21 33.08993576017131 35.0 32.0 37.0 26.0 37.0 22 34.111527480371166 35.0 33.0 37.0 30.0 37.0 23 32.94597608850821 35.0 32.0 37.0 26.0 37.0 24 32.842969307637404 35.0 32.0 37.0 26.0 37.0 25 33.09925945753034 35.0 32.0 37.0 26.0 37.0 26 34.60902926481085 37.0 32.0 39.0 28.0 39.0 27 35.12464311206281 37.0 33.0 39.0 28.0 39.0 28 35.785019628836544 37.0 35.0 39.0 30.0 39.0 29 36.18544789436117 37.0 35.0 39.0 32.0 39.0 30 36.39413811563169 37.0 35.0 39.0 32.0 39.0 31 37.887089578872235 39.0 37.0 40.0 33.0 41.0 32 38.22876516773733 40.0 37.0 40.0 34.0 41.0 33 38.53225374732334 40.0 38.0 40.0 36.0 41.0 34 38.657075303354745 40.0 38.0 40.0 36.0 41.0 35 38.66595289079229 40.0 38.0 40.0 36.0 41.0 36 38.70244468236974 40.0 38.0 40.0 35.0 41.0 37 38.59506602426838 40.0 38.0 40.0 35.0 41.0 38 38.42045860099929 40.0 38.0 40.0 35.0 41.0 39 38.22591006423983 40.0 37.0 40.0 34.0 41.0 40 37.98750892219843 40.0 37.0 40.0 34.0 41.0 41 37.75691470378301 40.0 37.0 40.0 33.0 41.0 42 37.488490364025694 39.0 36.0 40.0 33.0 41.0 43 37.371654175588866 39.0 36.0 40.0 33.0 41.0 44 37.275651320485366 39.0 36.0 40.0 33.0 41.0 45 37.21622947894361 39.0 36.0 40.0 33.0 41.0 46 37.230415774446826 39.0 36.0 40.0 33.0 41.0 47 37.21560492505353 39.0 36.0 40.0 33.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.0434490131614993 1101 2 0.5031079095457685 1101 3 0.5816034553530471 1101 4 0.7799489517348093 1101 5 0.26352331200089196 1101 6 0.7717695990740339 1101 7 1.0612106876216103 1101 8 0.6895822297538032 1101 9 0.7440209172716905 1101 10 0.609173601816984 1101 11 0.9118900175724107 1101 12 0.09785483299087616 1101 13 0.33073771757885595 1101 14 0.010533762480143594 1101 15 0.19903034002707898 1101 16 -0.554657671557294 1101 17 -0.18205465598300918 1101 18 0.29175839167702833 1101 19 0.27472096001311996 1101 20 0.4298517344007209 1101 21 0.8805120896087288 1101 22 0.2618459950476364 1101 23 -0.034695140722284634 1101 24 -0.45387521974586775 1101 25 -0.17355493444635073 1101 26 -1.0108497168293198 1101 27 -0.472951916329329 1101 28 -0.2308178989011367 1101 29 1.2059953829173509 1101 30 0.02488213539312767 1101 31 1.0110529624554374 1101 32 0.5708359794065174 1101 33 0.8096462190819409 1101 34 0.686253200682458 1101 35 0.5922055447216081 1101 36 0.9075553983300537 1101 37 0.25987926331747957 1101 38 0.6593886529310637 1101 39 0.7986976886523678 1101 40 0.3821840953668456 1101 41 0.14211857746211365 1101 42 0.32761236135954164 1101 43 -0.12078263743440232 1101 44 -0.4238666114182621 1101 45 0.4583742812128335 1101 46 0.7152414694596843 1101 47 0.6552706009808702 1102 1 0.2931101266234464 1102 2 0.3611724256748019 1102 3 0.33429162739606255 1102 4 1.0003790592616966 1102 5 1.5581469679148725 1102 6 1.4298341152030645 1102 7 -0.034488237109577824 1102 8 -0.08138551218167578 1102 9 1.028967153830834 1102 10 0.7435822039675202 1102 11 1.1086642111207965 1102 12 1.0871021448188287 1102 13 1.5543936315573461 1102 14 0.8578455904371296 1102 15 0.715159372285143 1102 16 1.4066326510233509 1102 17 1.2620313655223683 1102 18 0.051973445440470556 1102 19 0.053215583669032895 1102 20 -0.3927289107605709 1102 21 -0.09153092114395633 1102 22 -1.0725626071029026 1102 23 -0.5830822374964768 1102 24 -1.9861332842619959 1102 25 -2.493985041973229 1102 26 -1.8409572437110384 1102 27 -1.2460701958992217 1102 28 -0.7663017698688819 1102 29 -0.9036820364374876 1102 30 -0.5708167893380605 1102 31 -0.340559940770369 1102 32 -1.1646478915612235 1102 33 -0.2989559314556942 1102 34 -0.16535970254334842 1102 35 0.4857539318183868 1102 36 0.5978779789752124 1102 37 -0.22291643560725305 1102 38 0.555087577662249 1102 39 0.5664396241362368 1102 40 0.91229162224856 1102 41 0.6872798677846959 1102 42 0.4555693506068579 1102 43 0.5437334915978553 1102 44 0.4460258617000221 1102 45 1.0465463242235842 1102 46 1.1647038350510783 1102 47 0.9789265149593618 1103 1 -1.0476156798281693 1103 2 0.35310790954576987 1103 3 -0.42881321131361716 1103 4 -1.042967714931855 1103 5 -1.3843933546657752 1103 6 -2.1094804009259676 1103 7 -1.5304559790450583 1103 8 -2.0854177702461953 1103 9 -1.2976457493949738 1103 10 -1.0054097315163517 1103 11 -0.8006099824275879 1103 12 -1.6104785003424595 1103 13 -0.26717894908781403 1103 14 -1.8165495708531907 1103 15 -1.457219659972921 1103 16 -1.6296576715572968 1103 17 -0.7591379893163435 1103 18 0.23342505834369476 1103 19 -0.12319570665354718 1103 20 0.4298517344007209 1103 21 1.184678756275396 1103 22 0.27017932838096925 1103 23 -0.7846951407222846 1103 24 -1.2351252197458678 1103 25 0.29936173222031925 1103 26 -0.49418305016265407 1103 27 -0.5958685829959975 1103 28 -0.2370678989011381 1103 29 -1.7877546170826477 1103 30 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20 0.8975936698845928 1104 21 0.7149206917592679 1104 22 -0.04030454258677452 1104 23 1.8362726012131958 1104 24 -0.30871392942328413 1104 25 -0.2359205258442003 1104 26 0.9977524337083139 1104 27 0.5603814170040025 1104 28 0.8788595204537017 1104 29 0.967285705498 1104 30 0.8162799848554911 1104 31 0.8529884463264068 1104 32 0.7708359794065203 1104 33 0.6365279395120496 1104 34 0.415285458746979 1104 35 0.3244636092377391 1104 36 -0.273089762960268 1104 37 0.0674061450379071 1104 38 0.07121660992031309 1104 39 -0.014205537154083459 1104 40 -0.2167406358159525 1104 41 0.3646992226234005 1104 42 -0.15733387519959763 1104 43 -1.1982019922731126 1104 44 -1.6184902673322412 1104 45 -1.2115181919054478 1104 46 -1.415941326239242 1104 47 -1.8597831624599905 1105 1 1.2931101266234464 1105 2 -1.509795316260682 1105 3 0.463323885460575 1105 4 0.9358629302294332 1105 5 1.203308258237449 1105 6 0.7201566958482246 1105 7 0.7719633757936535 1105 8 0.8218402942699328 1105 9 1.028967153830834 1105 10 1.0984209136449365 1105 11 1.46350292079822 1105 12 0.9903279512704444 1105 13 0.6189097605896023 1105 14 0.5030068807597132 1105 15 0.457094856156111 1105 16 -0.1417544457508484 1105 17 0.6491281397159128 1105 18 -0.27060719972082126 1105 19 0.3112800997980649 1105 20 -0.19918052366379513 1105 21 -0.2205631792084759 1105 22 -0.6209497038770948 1105 23 -0.647598366528733 1105 24 0.07838284477026036 1105 25 0.31246657092999897 1105 26 -0.06676369532394233 1105 27 -0.11703793783470928 1105 28 -1.572753382772106 1105 29 -1.6456175203084555 1105 30 -1.5708167893380605 1105 31 -0.856688973028433 1105 32 -1.0033575689805758 1105 33 -1.2021817379073099 1105 34 -0.7137467993175406 1105 35 -0.3206976810848374 1105 36 -0.20857363392801176 1105 37 -0.5132390162524132 1105 38 -0.05781564814420648 1105 39 0.2761170434910767 1105 40 0.8155174287001756 1105 41 0.30018309359114426 1105 42 0.8104080602842743 1105 43 0.7372818786946311 1105 44 1.0911871520225986 1105 45 0.8529979371268155 1105 46 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35 -0.4653702128541468 1106 36 -0.2985052077305568 1106 37 -0.22193891850070457 1106 38 -0.02849013494772379 1106 39 -0.3406962507415727 1106 40 -0.5481189349361912 1106 41 -0.048790513446981265 1106 42 -0.16026642651924305 1106 43 -0.24805536470712752 1106 44 -0.002654490206140281 1106 45 -0.4598075369689809 1106 46 -0.41203125781304806 1106 47 -0.9174566717464074 1107 1 2.251953285689069 1107 2 1.930694116442325 1107 3 0.6390747197208668 1107 4 1.2339719402405578 1107 5 1.1209945763687088 1107 6 0.9303902887292068 1107 7 0.8531647106101161 1107 8 1.7550994711331143 1107 9 0.5428714919843358 1107 10 0.7068747512422675 1107 11 0.1498210520551737 1107 12 0.14383184448512765 1107 13 -0.2186875697774724 1107 14 0.24271767052611892 1107 15 0.474892408992595 1107 16 1.0039630180978776 1107 17 -0.8636638513853114 1107 18 0.20325264455059155 1107 19 -0.24711812044665038 1107 20 0.2057138033662369 1107 21 -0.08040745062115917 1107 22 -1.208268947481102 1107 23 -0.5864192786533167 1107 24 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-0.35983243376078633 2213 18 -0.2804638305451945 2213 19 -0.2030568177646579 2213 20 -0.18125937671038983 2213 21 0.3027343118309531 2213 22 0.17295710615874782 2213 23 0.2708604148332725 2213 24 0.29612478025413225 2213 25 0.4486672877758764 2213 26 -0.6886274946070969 2213 27 -0.8285074718848833 2213 28 -0.5530401211233595 2213 29 -0.1940046170826477 2213 30 -0.6362289757179838 2213 31 -0.13894703754456117 2213 32 -0.22916402059347973 2213 33 0.4707573301930523 2213 34 0.23069764512690227 2213 35 -0.12446112194505332 2213 36 0.19644428721893803 2213 37 0.04321259665081101 2213 38 -0.40727801373560624 2213 39 -0.040191200236520785 2213 40 0.31551742870017563 2213 41 -0.013436978093444907 2213 42 -0.3723876386404541 2213 43 -0.7985604152121795 2213 44 -0.29053327808492924 2213 45 -0.6694034965649465 2213 46 0.09301924723745714 2213 47 -0.005840510130241228 2214 1 -0.004852521933429443 2214 2 -1.9758394588752815 2214 3 -0.9008526849978296 2214 4 -0.8077703465108002 2214 5 -1.9978801967710353 2214 6 -0.7229672430312277 2214 7 -0.9580875579924282 2214 8 -1.0525230334040927 2214 9 -1.2647510125528711 2214 10 -0.32942288941109155 2214 11 0.19610054388819975 2214 12 0.34346886807859534 2214 13 -0.8938236859299167 2214 14 -0.7491153603268756 2214 15 -0.9325486073413387 2214 16 -0.8862366189257145 2214 17 -0.41538798931634346 2214 18 -0.009995994287884002 2214 19 0.4343700828201378 2214 20 0.9035359449270359 2214 21 -0.015979138461446496 2214 22 1.000442486275709 2214 23 -0.45574777230122976 2214 24 -0.5854541671142854 2214 25 -0.8174145835691533 2214 26 -0.8494462080573939 2214 27 0.2182761538461051 2214 28 0.32707683794096454 2214 29 -0.56242566971423 2214 30 -0.07336347864195858 2214 31 0.16368454140280875 2214 32 -0.04495349427769213 2214 33 -0.5833362370584112 2214 34 -0.6347994308964857 2214 35 -0.26042603422575894 2214 36 -0.4292867069331052 2214 37 -0.49626108755971643 2214 38 -0.5213131014549006 2214 39 -0.5065654692423749 2214 40 -0.342377308141927 2214 41 0.01580278798842727 2214 42 -0.15308939302641988 2214 43 -0.8892036900659832 2214 44 -0.08877889212001122 2214 45 -0.1767134380854145 2214 46 0.28015375016143196 2214 47 0.030709197472098992 2215 1 0.750995431282945 2215 2 -0.4802254237875623 2215 3 0.5260478997974971 2215 4 0.4466156184014807 2215 5 0.3413010897786677 2215 6 0.8106584879629182 2215 7 0.2889884653993846 2215 8 0.23402667419824752 2215 9 -0.3948679716171952 2215 10 0.414729157372534 2215 11 -0.21033220464980928 2215 12 -0.23547850034245954 2215 13 0.5974043842455217 2215 14 0.9994226513690307 2215 15 -0.27319188219514245 2215 16 -0.004657671557296794 2215 17 0.7512786773503208 2215 18 0.05286950278813762 2215 19 0.15805429334645282 2215 20 0.12429617884516375 2215 21 0.497178756275396 2215 22 0.6451793283809693 2215 23 0.29863819261105107 2215 24 0.46279144692079655 2215 25 -0.35688826777968075 2215 26 0.061372505392903065 2215 27 0.2548258614484453 2215 28 -0.19192901001225238 2215 29 0.7226620495840166 2215 30 1.002659913170902 2215 31 0.6388307402332174 2215 32 0.8263915349620774 2215 33 0.1652017746374952 2215 34 0.4529198673491237 2215 35 0.292205544721611 2215 36 0.5853331761078309 2215 37 0.07099037442858958 2215 38 0.3982775418199509 2215 39 0.7375865775412507 2215 40 0.5655174287001756 2215 41 0.3198963552398908 2215 42 0.5720568058039888 2215 43 0.7292173625655991 2215 44 -0.09608883364047927 2215 45 0.7472631701017249 2215 46 0.8707970250152357 2215 47 0.32749282320308737 2216 1 0.3898843201718307 2216 2 2.3031079095457727 2216 3 0.7482701220197185 2216 4 0.7132822850681464 2216 5 0.4635233120008948 2216 6 0.571769599074031 2216 7 0.2945440209549446 2216 8 -0.4104177702461982 2216 9 0.5773542506050262 2216 10 -0.457493064849686 2216 11 0.4118900175724107 2216 12 0.7978548329908719 2216 13 -0.40259561575447833 2216 14 0.2772004291468093 2216 15 0.49903034002707614 2216 16 -0.38799100489062965 2216 17 0.7179453440169894 2216 18 -0.07490827498963881 2216 19 0.27472096001311996 2216 20 -0.003481598932612684 2216 21 1.1471787562753946 2216 22 1.2618459950476364 2216 23 1.231971525944381 2216 24 2.0794581135874637 2216 25 1.593111732220322 2216 26 1.8558169498373474 2216 27 1.7937147503373367 2216 28 0.2691821010988633 2216 29 1.1726620495840194 2216 30 0.35821546872645627 2216 31 1.0443862957887688 2216 32 0.5708359794065174 2216 33 0.8429795524152723 2216 34 -0.1470801326508706 2216 35 0.5588722113882767 2216 36 0.574222064996718 2216 37 -0.00678740334918615 2216 38 -0.040611347068939097 2216 39 0.4986976886523635 2216 40 0.6821840953668428 2216 41 1.0421185774621122 2216 42 0.8942790280262116 2216 43 1.179217362565602 2216 44 0.8094667219150722 2216 45 0.9250409478795021 2216 46 1.3819081361263486 2216 47 1.8219372676475345 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 1.0 25 1.0 26 13.0 27 42.0 28 105.0 29 242.0 30 417.0 31 682.0 32 890.0 33 1186.0 34 2052.0 35 3836.0 36 5449.0 37 4134.0 38 3177.0 39 189.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 99.15239114917915 0.19182726623840116 0.3122769450392577 0.34350463954318344 2 2.1145610278372593 96.32405424696645 0.9903640256959315 0.5710206995003569 3 0.08029978586723768 0.11598857958600998 0.1873661670235546 99.61634546752319 4 0.5754817987152034 0.704853675945753 98.4653818700928 0.2542826552462527 5 0.9412919343326196 95.50767309064953 1.7978229835831547 1.7532119914346895 6 0.23197715917201997 0.08476088508208424 0.45057102069950034 99.23269093504639 7 98.69289793004997 0.6513204853675946 0.18290506780870808 0.47287651677373305 8 1.070663811563169 0.6870092790863669 97.56870092790864 0.6736259814418273 9 26.668451106352602 26.226802284082794 22.613311920057104 24.491434689507493 10 27.92772696854785 27.570823109524873 20.397055543163063 24.104394378764223 11 27.29300499643112 30.411313347608846 21.266059957173447 21.02962169878658 12 24.848322626695218 26.806745182012847 21.680942184154176 26.663990007137762 13 30.50053533190578 27.578515346181298 20.78872234118487 21.132226980728053 14 28.002319771591722 26.72644539614561 21.243754461099215 24.027480371163453 15 25.298893647394717 27.62758743754461 22.689150606709493 24.384368308351178 16 29.153283369022127 25.713775874375443 19.94557458957887 25.187366167023555 17 22.82298358315489 26.90935046395432 25.44164882226981 24.826017130620983 18 42.82718307973763 27.923787425817682 12.806211235553969 16.442818258890725 19 19.633297644539617 23.148643825838686 38.552819414703784 18.665239114917917 20 15.494088779834932 18.951594914120008 48.26678563461967 17.287530671425387 21 14.311206281227696 25.517487508922198 43.562633832976445 16.608672376873663 22 14.904532476802284 24.660956459671663 17.91131334760885 42.523197715917206 23 34.342081771112305 27.383502945902517 16.73361899660775 21.540796286377432 24 21.968412599268312 31.605246720799503 26.23806549478005 20.188275185152136 25 20.182012847965737 26.253568879371876 28.894539614561026 24.669878658101357 26 20.65488936473947 25.379193433261953 29.166666666666668 24.799250535331907 27 22.024446823697357 26.070663811563172 30.955567451820126 20.949321912919345 28 21.332976445396145 28.310135617416133 29.8313704496788 20.525517487508925 29 21.877230549607425 26.021591720199854 30.754817987152034 21.346359743040686 30 20.92701641684511 24.660956459671663 31.8923982869379 22.519628836545323 31 21.002855103497502 26.378479657387583 31.69164882226981 20.92701641684511 32 20.842255531763026 27.16363311920057 30.005353319057814 21.988758029978587 33 21.654175588865098 29.22466095645967 29.362955032119913 19.758208422555317 34 21.783547466095644 28.653640256959317 28.787473233404707 20.775339043540328 35 21.680942184154176 29.072983583154887 30.91541755888651 18.330656673804423 36 21.955028107432856 27.830820023199788 30.342642990987777 19.871508878379586 37 21.814775160599574 28.002319771591722 31.47751605995717 18.705389007851533 38 20.99393290506781 29.73322626695218 31.905781584582442 17.367059243397573 39 20.570128479657388 30.415774446823697 29.800142755174875 19.21395431834404 40 20.556745182012847 28.63133476088508 30.40239114917916 20.409528907922912 41 20.401516841400845 30.140530894490297 29.788088333705108 19.669863930403746 42 20.47644539614561 30.121341898643827 29.93843683083512 19.463775874375447 43 19.79389721627409 29.380799428979298 30.848501070663815 19.976802284082797 44 20.554117961988045 29.138038725796378 31.413402337824575 18.894440974391006 45 20.239157594145993 29.863466000356954 30.706764233446364 19.190612172050685 46 22.149357601713064 29.349571734475376 30.366702355460383 18.134368308351178 47 20.758420700423823 28.628150791880437 31.594914120008923 19.018514387686817 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 2.5 8 5.0 9 5.0 10 7.0 11 9.0 12 19.0 13 29.0 14 36.0 15 43.0 16 69.0 17 95.0 18 126.5 19 158.0 20 186.5 21 215.0 22 253.5 23 292.0 24 323.0 25 354.0 26 354.0 27 419.0 28 484.0 29 729.0 30 974.0 31 826.0 32 678.0 33 726.0 34 774.0 35 881.5 36 989.0 37 1067.0 38 1145.0 39 1240.5 40 1336.0 41 1407.5 42 1479.0 43 1479.0 44 1580.0 45 1681.0 46 1768.5 47 1856.0 48 1863.5 49 1871.0 50 1814.5 51 1758.0 52 1681.0 53 1604.0 54 1447.0 55 1290.0 56 1237.5 57 1185.0 58 1185.0 59 939.5 60 694.0 61 572.0 62 450.0 63 357.0 64 264.0 65 206.5 66 149.0 67 146.0 68 143.0 69 113.5 70 84.0 71 180.5 72 277.0 73 152.0 74 27.0 75 27.0 76 22.0 77 17.0 78 9.5 79 2.0 80 2.0 81 2.0 82 2.0 83 2.0 84 1.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.004461099214846539 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.02230549607423269 19 0.0 20 0.004461099214846539 21 0.0 22 0.0 23 0.05353319057815846 24 0.008922198429693077 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.008922198429693077 37 0.0 38 0.0 39 0.0 40 0.0 41 0.004461099214846539 42 0.0 43 0.0 44 0.008922198429693077 45 0.017844396859386154 46 0.0 47 0.004461099214846539 >>END_MODULE >>Sequence Length Distribution pass #Length Count 47 22416.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.75678087080657 #Duplication Level Percentage of deduplicated Percentage of total 1 84.50108797015852 60.635260528194145 2 9.53061858874728 13.677730192719487 3 2.7603357165060616 5.942184154175589 4 1.1936586882188374 3.4261241970021414 5 0.5470935654336339 1.9628836545324768 6 0.3978862294062791 1.7130620985010707 7 0.19894311470313955 0.9992862241256246 8 0.19272614236866645 1.1063526052819415 9 0.12433944668946223 0.8029978586723768 >10 0.5160087037612683 5.763740185581727 >50 0.018650917003419334 0.8877587437544611 >100 0.018650917003419334 3.082619557458958 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GACTACGTACGCGCCCTACTACGTTTTTTTTTTTTTTTTTTTTTTTT 361 1.6104568165596003 No Hit GGCTTCGTCCATGGCCTGGTCACAGCGGGCTCGGGCGTGGCCATCGG 199 0.8877587437544611 No Hit GACTACGTTATCGCACCCTTGGCTCCTCGAGCCAGCGTCGAGTTCTT 131 0.5844039971448965 No Hit GACTACGTCGGAGTCCCCGACTCACTATAGTGAGTCGTATTACCGGC 97 0.43272662384011423 No Hit GACTACGTATATAGAATGTTTAGGACAGCTCAACTTATCACCAGATC 51 0.22751605995717344 No Hit GACTACGTGCAGAAGGGTGATGATCGGAGTGTCGTCACCCGGGAAGT 51 0.22751605995717344 No Hit GACTACGTGACCACTGAGCCAACGTCACATTAGGGTCCGCAAGCAGG 43 0.19182726623840116 No Hit GACTACGTCGATTCGTCCTTTCATCCAAACTTCCCAGAAGCGAATCT 40 0.17844396859386152 No Hit GACTACGTCACCAATCAGCTTCACCACAGCTCAGAAGAGGGTGTACA 37 0.16506067094932192 No Hit GACTACGTGGTCAATAAACGATCTCGACTGCACAGTCAGTGACTACG 36 0.16059957173447537 No Hit GACTACGTCACCTGCGTATCTCTATACACGTGGCAGTGACTACGTTT 33 0.14721627408993576 No Hit GACTACGTGTATGTGGGGTTTCCTTGGTAGGTTTTGTTTCTTTTGTA 32 0.14275517487508924 No Hit GACTACGTTGAGGCCCTTCCGACGATCTCGAAGATGAGGCCAGTGAC 32 0.14275517487508924 No Hit GACTACGTTTAGTTTTAGAAGAAATGTAACACTGTGTAAGAAAACAC 28 0.12491077801570308 No Hit GACTACGTGAAACCGTAATTTCGAAGAGATGAGGAAGGCTGGTATCT 24 0.10706638115631692 No Hit GACTACGTACGGCGAACACGCAAGCAGGCTGAATGGGATGCTCAGAA 23 0.10260528194147038 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.004461099214846539 0.0 0.0 17 0.0 0.0 0.02230549607423269 0.0 0.0 18 0.0 0.0 0.031227694503925766 0.0 0.0 19 0.0 0.0 0.12044967880085652 0.0 0.0 20 0.0 0.0 0.13383297644539616 0.0 0.0 21 0.0 0.0 0.1516773733047823 0.0 0.0 22 0.0 0.0 0.16059957173447537 0.0 0.0 23 0.0 0.0 0.16506067094932192 0.0 0.0 24 0.0 0.0 0.1873661670235546 0.0 0.0 25 0.0 0.0 0.24982155603140613 0.0 0.0 26 0.0 0.0 0.26320485367594576 0.0 0.0 27 0.0 0.0 0.27658815132048536 0.0 0.0 28 0.0 0.0 0.3122769450392577 0.0 0.0 29 0.0 0.0 0.33458244111349034 0.0 0.0 30 0.0 0.0 0.3970378301213419 0.0 0.0 31 0.0 0.0 0.4773376159885796 0.0 0.0 32 0.0 0.0 0.5844039971448965 0.0 0.0 33 0.0 0.0 0.8119200571020699 0.0 0.0 34 0.0 0.0 0.9992862241256245 0.0 0.0 35 0.0 0.0 1.1331192005710207 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTCGT 35 2.3737448E-7 41.000004 5 ACGTTCC 35 2.3737448E-7 41.000004 5 ACGTTCA 35 2.3737448E-7 41.000004 5 ACGTTAT 35 2.3737448E-7 41.000004 5 ACGTTAA 35 2.3737448E-7 41.000004 5 TACGTTA 95 0.0 41.000004 4 TACGTAC 140 0.0 41.000004 4 ACGTGAA 35 2.3737448E-7 41.000004 5 GTCGGAG 35 2.3737448E-7 41.000004 7 GCCCTAC 20 0.0010889686 41.0 13 ACAGCGG 20 0.0010889686 41.0 22 CGTTCCT 20 0.0010889686 41.0 6 CGTTCAC 20 0.0010889686 41.0 6 TGGTCAC 20 0.0010889686 41.0 17 CCTACTA 20 0.0010889686 41.0 15 CGGAGTC 30 3.930156E-6 41.0 9 CGTTATC 30 3.930156E-6 41.0 6 ACGTCTT 40 1.4375473E-8 41.0 5 ACGTCTG 25 6.5291686E-5 41.0 5 ACGTCTA 30 3.930156E-6 41.0 5 >>END_MODULE