FastQCFastQC Report
Thu 26 May 2016
SRR2099240_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099240_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526550
Sequences flagged as poor quality0
Sequence length47
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCGACCATGACGCATGATTGGTGGCCATGTAGGTCTCCTCTCGCT24520.4656727756148514No Hit
TGACGATATTTGCGATTATCCATGCAACAGGGTTCAGTGACGATCTT24450.4643433672015953No Hit
TGACGACCTGTACGATCAAATTGACAGGAAAACTACCAGAAGGCATT20070.381160383629285No Hit
TGGCCATCGGATCACACAGTTTTTGTTGTTGTTTTGGTTTTTTTTTT18510.35153356756243476No Hit
TGACGATCATCCGGGCAGCGGCGCGTGCGCGGGCGGGGCCCGCGTGC16360.3107017377267116No Hit
TGACGATCGTATTTGACCAGCCACCGCGGCAGCGATGGCCAATGGCG14930.28354382299876557No Hit
TGCCGACCATGACGCATGGATTGGTGGCCATGTAGGTCTCCTCTCGC14050.2668312600892603No Hit
TGACGATCGGGGACCGAATCCATGTCGGTCACTTCAGTGACCGACAT13210.2508783591301871No Hit
TGACCAGCCGAATTCCAGTGGCGGCTGGTGGGACGCAGCCAGGCCGA13110.24897920425410694No Hit
TGACGAACACAGAATACGGGCAGTCTGTCTATTTCCTTGCCAGGCAA11630.22087171208812081No Hit
TGACGATCGTAGCTACTGTGGATCACGCCTGTAATCCCAGCACTTTG11390.21631374038552845No Hit
TGACGATCGAGCACCTAGCGTTGACGCCCGCGGATGCCACTCGCACT11370.21593390941031243No Hit
TGACGATCGTAGTGGATGTAAGCTTTTAAATGACTTAATTATTTTCA10080.19143481150887853No Hit
TGACGAACACAGAATACGGCAGTCTGTCTATTTCCTTGCCAGGCAAG8630.16389706580571645No Hit
TGACGATCGTATTTGACGCAGCCACCGCGGCAGCGATGGCCAATGGC7390.14034754534232266No Hit
TGACGATCTTTTGCTCTCAGTCCGACGATCCATGCCTCATGGATCGT6760.12838286962301776No Hit
TGACGATCAGAGATACAAGATCAGTGACGATCTTTCTCTCATGGATC6480.12306523596999336No Hit
TGACGATCAAAAGGCTTCAGTCCGACGATCCATGGATCGCGGACTGT5930.11261988415155255No Hit
TGACGATCAAAGGTTGTGTTCGTCAGTGACGATCCGTCCATGGATCG5750.1092014053746083No Hit
TGACGATCCACGCAATGAGAGCCGGAGAACTCGGGAGGGAGACGGGG5630.10692241952331212No Hit
TGACGATCACGGAGGGTGGGGACAGTAATGGTATAAGGAGCAAAGAT5600.10635267306048808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAC401.5445039E-841.0106477
TCTAGCG304.096397E-641.0106477
GTTCGCA304.096397E-641.0106475
TCGCTTA304.096397E-641.0106477
TCACTTA200.001110200241.0106477
TCGTAGC3150.041.0106477
CACGTTG200.001110200241.0106478
ATTTGCG3200.041.0106478
CGGAGCG401.5445039E-841.0106478
TCGCGTA304.096397E-641.0106477
ATCGCCT1750.041.0106476
CCCCTTG900.041.0106438
GCGCGAA459.549694E-1041.0106439
ATCAGCC506.002665E-1141.0106436
CCGGACC700.041.0106438
CCTCGCA256.723559E-541.0106438
TCGTGTG256.723559E-541.0106437
TACGATA700.041.0106439
TAATTCG256.723559E-541.0106439
ATCCCTT1300.041.0106436
Produced by FastQC (version 0.11.5)