FastQCFastQC Report
Thu 26 May 2016
SRR2099239_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099239_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12153
Sequences flagged as poor quality0
Sequence length47
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACGATATTTGCGATTATCCATGCAACAGGGTTCAGTGACGATCTT640.5266189418250637No Hit
TGCCGACCATGACGCATGATTGGTGGCCATGTAGGTCTCCTCTCGCT510.41964946926684765No Hit
TGACGACCTGTACGATCAAATTGACAGGAAAACTACCAGAAGGCATT460.3785073644367646No Hit
TGGCCATCGGATCACACAGTTTTTGTTGTTGTTTTGGTTTTTTTTTT380.3126799967086316No Hit
TGCCGACCATGACGCATGGATTGGTGGCCATGTAGGTCTCCTCTCGC360.2962231547765984No Hit
TGACGATCGTATTTGACCAGCCACCGCGGCAGCGATGGCCAATGGCG330.27153789187854854No Hit
TGACCAGCCGAATTCCAGTGGCGGCTGGTGGGACGCAGCCAGGCCGA320.2633094709125319No Hit
TGACGATCTTATTATAAAATCCAGGACCTGGAAAAGAAGGAGGGACG300.24685262898049865No Hit
TGACGATCGACATACGCGGTTCGATCAGGCTACTACAAAGTTCTGGG280.23039578704846542No Hit
TGACGATCGGGGACCGAATCCATGTCGGTCACTTCAGTGACCGACAT280.23039578704846542No Hit
TGACGAACACAGAATACGGGCAGTCTGTCTATTTCCTTGCCAGGCAA280.23039578704846542No Hit
TGACGATCGTAGTGGATGTAAGCTTTTAAATGACTTAATTATTTTCA250.20571052415041552No Hit
TGACGATCGTATTTGACTCAGCCACCGCGGCAGCGATGGCCAATGGC210.17279684028634903No Hit
TGACGATCTACACGTATGATCCATGCTAACTGGATCATCGGACTGTA210.17279684028634903No Hit
TGACGATCATCCGGGCAGCGGCGCGTGCGCGGGCGGGGCCCGCGTGC200.16456841932033242No Hit
TGACGATCGTATTTGACGCAGCCACCGCGGCAGCGATGGCCAATGGC200.16456841932033242No Hit
TGACGATCGTAGCTACTGTGGATCACGCCTGTAATCCCAGCACTTTG200.16456841932033242No Hit
TGACGATCGAGCACCTAGCGTTGACGCCCGCGGATGCCACTCGCACT190.1563399983543158No Hit
TGACGATCGGATACGACGTTTTGAGGGGTTCACTTTTCTGGTTGAAA180.1481115773882992No Hit
TGACGATCATGTGCGGTGTTTCACTAGATCGTGTTATGGACGATGAC180.1481115773882992No Hit
TGACGAACACAGAATACGGCAGTCTGTCTATTTCCTTGCCAGGCAAG170.13988315642228258No Hit
TGCCGACCATGACGCATTGATTGGTGGCCATGTAGGTCTCCTCTCGC170.13988315642228258No Hit
TGACGATCCATGTTCCGGTTTCTTGATTCTGACCCTGTATAGCTTCC170.13988315642228258No Hit
CGCCGATCAGAGATACAAGATCAGTGACGATCTTTCTCTCATGGATC160.13165473545626594No Hit
TGACGATCTTGCATCAGATTTCACTAATTCCAATACTTATTATTATT150.12342631449024932No Hit
TGACGATCAAGCGAACGGTTGCACCTCCTCCTAAAGGTTGGGCTTCT150.12342631449024932No Hit
TGACGATCCGACAGTTAATTTCCGAGGCCATCCGCATCGTCGTCATC150.12342631449024932No Hit
TGACGATCCTGCACACCGTTTCGCTCGGGTCTGCGGGTTATAGCTTT140.11519789352423271No Hit
TGACGATCGCCCTACGCTCTCAAACTCCTGACCTCATAATCCGCCTG130.10696947255821607No Hit
TGACGATCAGTGTCAGGGTTGCGATTGAGGAATGGATTGGTACCTTT130.10696947255821607No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGA200.00107138540.9835425
CGATATT200.00107138540.983544
ATCCAGG200.00107138540.983546
GCGATTA200.00107138540.9835412
TGCGATT200.00107138540.9835411
AGGGTTC200.00107138540.9835429
GATCGTA401.3504177E-840.983545
GATCGGG352.2621316E-740.983545
CCATGCA200.00107138540.9835420
GATCGAT303.7924492E-640.983545
GATCGAG303.7924492E-640.983545
GATCGAA200.00107138540.983545
GGTTCAG200.00107138540.9835431
CAACAGG200.00107138540.9835425
ACATTTC200.00107138540.9835416
GCAACAG200.00107138540.9835424
GAGTTCT303.7924492E-640.9835418
ATTATCC200.00107138540.9835415
CAGGGTT200.00107138540.9835428
GATCCTT256.36769E-540.983545
Produced by FastQC (version 0.11.5)