FastQCFastQC Report
Thu 26 May 2016
SRR2099238_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099238_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1925515
Sequences flagged as poor quality0
Sequence length47
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGGCAACTGCTGCTAGGTGGAACGGCTGCGTGCGTTCGCCCCGC62210.323082396138176No Hit
CTGATGCACGAGTCTAGATCAGCTGTTCACAGAAGTCCTGATGCATT55810.28984453509840225No Hit
CAGTTGCACGCGTTCAAGTTGGGGCGGAACACCACTTGCAGCGCCTG50200.2607094725307256No Hit
CTGATGCAGAAGGGAACAGAGGACTTGTCACCTTCTTTGCTTTCTAG19740.10251802764455224No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGCG256.746797E-540.99355713
GTCCCGT200.001113785140.989312
GATGCAC341100.040.692723
GATGCAG431100.040.6343153
ATGCACG94950.040.5734184
TGCACGA38450.040.3120775
GATGCAA617450.040.3013233
ATGCAAG151800.040.1948974
ATGCAGA155700.039.9654
ATGCAAC111650.039.866754
ATGCACT66550.039.834584
ATGCAGC88100.039.7485854
ATGCACC69950.039.6276864
TGCACGC32150.039.6022955
ATGCAGG109300.039.5796554
TGCATAG27250.039.5757335
TTGCACG12700.039.3908964
GCACGCG16500.039.389916
ATGCAAT117250.039.344084
ATGCACA121000.039.29394
Produced by FastQC (version 0.11.5)