FastQCFastQC Report
Thu 26 May 2016
SRR2099237_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099237_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40973
Sequences flagged as poor quality0
Sequence length47
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGGCAACTGCTGCTAGGTGGAACGGCTGCGTGCGTTCGCCCCGC1800.43931369438410656No Hit
CAGTTGCACGCGTTCAAGTTGGGGCGGAACACCACTTGCAGCGCCTG1350.32948527078807993No Hit
CTGATGCACGAGTCTAGATCAGCTGTTCACAGAAGTCCTGATGCATT1280.3124008493398091No Hit
CTGATGCAGAAGGGAACAGAGGACTTGTCACCTTCTTTGCTTTCTAG480.11715031850242842No Hit
CAGTTGCACGCGTTCAAGGTTGGGGCGGAACACCACTTGCAGCGCCT410.10006589705415761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACGT505.638867E-1141.05
TGCACGG352.4395558E-741.05
TGCACCG459.113137E-1041.05
TGCACAG459.113137E-1041.05
TGCACAA1350.041.05
TGCATAA1050.041.05
TTGCACG401.4890247E-841.04
AATCTAG200.001099646141.09
CAGTTGC352.4395558E-741.01
GCAAGTC553.6379788E-1241.06
TGCAATC750.041.05
TGCGTGC200.001099646141.030
CGCCCCG200.001099646141.040
TGCAAGT1000.041.05
TGCAAGG401.4890247E-841.05
TGCAACT352.4395558E-741.05
TGCAACC459.113137E-1041.05
CAACTGC200.001099646141.07
CAGTACG200.001099646141.07
CACAAAT200.001099646141.07
Produced by FastQC (version 0.11.5)