FastQCFastQC Report
Thu 26 May 2016
SRR2099236_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099236_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576537
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTCAATCGAAATTAGAACCCAAATGTCTGCTGGGCTTCT32160.12481870044947928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACG182650.036.8582954
ACGTCAC739550.036.8205683
CGTCAAC179050.036.7930074
ACGTCAA617800.036.730673
CGTCATA113800.036.7276654
CGTCACA179700.036.6702234
CGTCACT144650.036.5981564
TACGTCA2370050.036.5630232
CGTCAAT127650.036.5419164
ACGTCAG541200.036.5292243
CGTCAAG177050.036.5290684
GTCACCG62150.036.5176775
CGTCATC162350.036.4731754
GTCACGC67150.036.472685
CGTCACC246600.036.4049344
CGTCAGG146850.036.314574
CGTCAGC171500.036.254134
GTACGTC2431100.036.149611
AATCGCG2100.036.1460278
CGTCAAA143100.036.114984
Produced by FastQC (version 0.11.5)