FastQCFastQC Report
Thu 26 May 2016
SRR2099234_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099234_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708386
Sequences flagged as poor quality0
Sequence length42
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCGACTGCATTCTCCCGCAGTTCCCGCGTCGCCTCGGGCA30660.4328148777643827No Hit
CTACGAGTAGCAGTGATGATCTGGTCCTCTGATGGAACTCAT24030.3392218366822608No Hit
CTACGAGTCCTGGGCGCGGCCTGCTCCGTGGTGTGCGGAGCA17730.2502872727580726No Hit
CTACGAGTCGTCGGGTGGGGTGAGGAGGAGGTGTGGGATGTG14860.20977263808149793No Hit
CTACGACTGCGGAGTGCGGGTCATAAGCTTGCGTTGATTAAG13930.19664420245459396No Hit
CTACGAGTCCTAGAGCAATCTGACATTGCTCTGGCACCCAAT12800.18069244733803322No Hit
CTACGAGTCGGTGGTAATGAAATAAGGCAGAATTGCTTTATA11870.16756401171112925No Hit
CAGCGAGTCCGCGATTGCAGCAGCAATCGGCGTATACCCTGT11650.1644583602725068No Hit
CTACGAGTTCATGCGATGAGTGTTGGGTGCCATATTGACAAA10350.1461067835897378No Hit
CTACGAGTCGTCGGGGTGGGGTGAGGAGGAGGTGTGGGATGT9620.1358016674524906No Hit
CTGCGACTGCATTTCCCGCGTCGCCTCGGGCAGTTCGAAGGC8880.12535538534076054No Hit
CTACTAGTTTAGGGAATGCAATGGAATGGAATGGAATGGAAT8490.11984991233592984No Hit
CTACGAGTGTGCACGATCCCTTCTACGAGTTTTTTTTTTTTT8290.11702659284627308No Hit
CTACGAGTGATTGCTCCCCGCCCCCGGAGCCCCGCGGACGCT7520.10615681281109451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG251.4424165E-436.0271538
AGAACGT251.4424165E-436.0271539
GTAGCGT251.4424165E-436.0271537
TTATCGG551.6370905E-1136.027158
TCGCTAG356.932678E-736.027158
TCCGTAC356.932678E-736.027158
TTCGCGG551.6370905E-1136.027158
TCCGCGG2100.036.0271458
CGCGTAG551.6370905E-1136.0246054
AACGGAT251.4520413E-435.98644310
AGCGCGG200.002110462235.9864410
CCGTTTA900.035.9864411
ACGTCGC251.4526446E-435.983912
TACGATA453.445166E-935.973742
ACGAGTG166650.035.916523
ACGAGTA101050.035.8641743
CGAGTGT46150.035.8294534
ACGAGTC145550.035.7770963
CGAGTGG48650.035.728414
AGCGCGA4500.035.6268466
Produced by FastQC (version 0.11.5)