FastQCFastQC Report
Thu 26 May 2016
SRR2099233_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099233_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41182
Sequences flagged as poor quality0
Sequence length42
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGAGTCTATGAATAGAGTTGAGCCCTTTGAATGGCTATA1730.4200864455344568No Hit
CTACGAGTCGGCGTGGGCTGCCCCTATAGGCAACTTCTTTGC1360.3302413675877811No Hit
CTACGAGTTTTAGATTCGTCCTCCTCAGCCTTGTCACTGGGA1230.2986741780389491No Hit
CTACGAGTGATGGTCTCGACGGGCTAGCACACATCTGGTTGA1080.26225049779029674No Hit
CTACGAGTAACCGGTTCTTCAAGCACATCTCGCGCTGGCGTG1000.2428245349910155No Hit
CTACGAGTCCTAATTTCGGCTAGAGCCACCATACTAGCAGTG1000.2428245349910155No Hit
CTACGAGTCAGGGTTTCGCTCGGGTCTGCGGGTTATAGCTTT900.21854208149191395No Hit
CTACGAGTCGAAGTTTCTTCTATTGTATTTTCAAATAGCAGA800.19425962799281238No Hit
CTACGAGTACGTGTTTCGCCATGTTGGCCAGGCTGGTCTTGA760.18454664659317177No Hit
CTGCGACTGCATTCTCCCGCAGTTCCCGCGTCGCCTCGGGCA690.16754892914380068No Hit
CTACGAGTGCCGGATTTGCCATCGAAAGACGACCTCTACTTT680.16512068379389055No Hit
CTACGAGTACTAGTTAAGAACTGGAAGCAGTCAAACTAATGG650.15783594774416007No Hit
CTACGAGTGAGTGTTTACTTTGAAAAAATTAGAGTGTTCAAA610.14812296634451946No Hit
CTACGAGTAGCAGTGATGATCTGGTCCTCTGATGGAACTCAT610.14812296634451946No Hit
CTACGAGTTCTTGGTTCCCTGGCCGCCAGAAGATCCACATCT600.14569472099460928No Hit
CTACGAGTCGGCGGTGGGCTGCCCCTATAGGCAACTTCTTTG600.14569472099460928No Hit
CTACGAGTGCCCGTTTAAAGACCTCTTGGCCTCTGTGACCCT590.14326647564469913No Hit
CTACGAGTGGAGGATCCGGACTGTAGAACTCTGAACCCCTAT590.14326647564469913Illumina DpnII expression PCR Primer 2 (95% over 22bp)
CTACGAGTCGTTGTGGCGGCGACGACCCATTCGAACGTCTGC580.14083823029478898No Hit
CTACAAGTCCTAGTTTTGGGGATCTCAGTATCTCTTTCTCTA570.13840998494487883No Hit
CTACGAGTTTTCGTTTACTTTGAAAAAATTAGAGTGTTCAAA560.13598173959496868No Hit
CTACGAGTTCAAATCTCGACTGTAGAACTCTGAACCCCTATA560.13598173959496868No Hit
CTACGAGTCCAAGCTTCTCGCCATGTCTTCTCACAAGACTTT560.13598173959496868No Hit
CTACGAGTGGTCGATTAGATAGGGAAAAGATCGGGATGCTAC550.13355349424505852No Hit
CTACGAGTTGTTGCGACGGGCTAGCACACATCTGGTTGACTA530.1286970035452382No Hit
CTACGAGTGCGCGTTTAAGACCAGGCTGGTGAGTTGGCTCAG520.12626875819532804No Hit
CTACGAGTTGTCGTCTTGGGGTTCTTTACACACACACACAAA510.1238405128454179No Hit
CTACGAGTATGTGTTTACTTTGAAAAAATTAGAGTGTTCAAA500.12141226749550775No Hit
CTACGAGTGCCTGTTACAAGACGGTGCTGGGGATGGCCCCAG500.12141226749550775No Hit
CTACGAGTAATTGTTTCATAACACAAAGATGTTTTTTACAAG480.11655577679568743No Hit
CTACGAGTTAGGATCTCGGTCAGCTGCCCCACAAGCTGCCCT470.11412753144577727No Hit
CTACGAGTCGTTGTATAGCTTCCCATCTTCTTTGAGAGTTGT470.11412753144577727No Hit
CTACGAGTGCCCGCCTAGCCTCAGGATGAGGTATATCTACGA460.11169928609586713No Hit
CTACGAGTGCGCTTTAAGACCAGGCTGGTGAGTTGGCTCAGC460.11169928609586713No Hit
CTACGAGTCCGTGGTTCGATCAGGCTACTACAAAGTTCTGGG450.10927104074595698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTCA200.002073613536.03528212
AATTTCG200.002073613536.03528212
AGGACCG251.4170425E-436.03528217
GGACCGC251.4170425E-436.03528218
TCGGCTA200.002073613536.03528216
TCGGTCT200.002073613536.03528216
TTCGATC200.002073613536.03528215
GACGGGG309.731089E-636.03528215
GTTTCGC251.4170425E-436.03528213
GGTTTCG200.002073613536.03528212
ACGGGGA309.731089E-636.03528216
AGGTTCG251.4170425E-436.03528212
GGCTAGA200.002073613536.03528218
TGTGGCG200.002073613536.03528212
GAATAGA200.002073613536.03528213
CGGTCTA200.002073613536.03528217
GGAATAG200.002073613536.03528212
TTCGGCT200.002073613536.03528215
TTTCGGC251.4170425E-436.03528214
CTACAAG502.2737368E-1035.9914971
Produced by FastQC (version 0.11.5)