FastQCFastQC Report
Thu 26 May 2016
SRR2099231_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099231_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47543
Sequences flagged as poor quality0
Sequence length42
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATGTCGTCCGTAAAGCTAGGGGCTGGTGAGATGGCTCAG2590.544769997686305No Hit
ACGATGTCATGCGTTTCAGTCTCGACGGGCTAGCACACATCT2480.5216330479776202No Hit
ACGATGTCTCTTGTTTAGTAGTTCCTGATCCCAGCTCTTTCC1690.3554676818879751No Hit
ACGATGTCCTGCGTGGACTCAAGCAGTCTCTCAACCCACAGG1520.31971057779273504No Hit
ACGATGTCGCACGTTTCAGTCTCGACGGGCTAGCACACATCT1250.262919883053236No Hit
ACGATGTCTGAGGATTGGAATAGAGACGTACCCTTAGCACAC1110.23347285615127358No Hit
ACGATGTCCATAGAAACGGATTGCAAGCAATCAGCACTGTAG1080.22716277895799591No Hit
ACGATGTCTGTGGTTTCTCAGAACGCTGCTGTGTGCTTTTTA1050.22085270176471825No Hit
ACGATGTCGCCCGGATAGTCTTGGGCGCTAACATTTTACAAC1010.21243926550701472No Hit
ACGATGTCAGTAGTTTTGCCGGCGCACAAGCACGATCGGCTC990.20823254737816294No Hit
ACGATGTCTCAAGTTTTGCCGGCGCACAAGCACGATCGGCTC920.1935090339271817No Hit
ACGATGTCTCTGGTAAGGACCGCCGCTTTCGCTCGGGTCTGC860.18088887954062638No Hit
ACGATGTCTCCCGTATCGCGCACGTAGTAGCCTAGAGCGCCA780.16406200702521928No Hit
ACGATGTCACAGGGGAAGAGGAGGCAGAAAGATTGTAGAAAC770.16195864796079337No Hit
ACGATGTCCACCGTTTACTTTGAAAAAATTAGAGTGTTCAAA760.15985528889636752No Hit
ACGATGTTTCGAGAAACGTGAAAAATGAGAAATGCACACTGA750.1577519298319416No Hit
ACGATGTAGTGCATTTCTACCATACCCTACACCACACTTTGC750.1577519298319416No Hit
ACGATGTCGCTGGAAAAGTTTTGCCGGCGCACAAGCACGATC720.15144185263866394No Hit
ACGATGTCCGAGGTTTCAGTCTCGACGGGCTAGCACACATCT700.14723513450981218No Hit
ACGATGTCAAGTGAATAGGAGTTTTCCTGTGCTTCCCTTAAA670.14092505731653449No Hit
ACGATGTCTCCCGTCTAACAACAACATCCACACTACTACTCC630.13251162105883094No Hit
ACGATGTCTCTTGTTTCAATAAAAGAAAAATAGAAGGGAAAG620.13040826199440506No Hit
ACGATGTCCAGCGAATAATGGAATAGGACCGCGGTTCTATTT590.1240981848011274No Hit
ACGATGTCGTTTGTTTCAGTCTCGACGGGCTAGCACACATCT590.1240981848011274No Hit
ACGATGTCGGGTAATACGACTCACTGTAGAACTCTGAACCCC570.11989146667227561No Hit
ACGATGTCGGGCATTTTGAGGTCTTAACTTCTTTCTTTTTAA570.11989146667227561No Hit
ACGATGTCAGGAGATCCAAGTGGGCCACATGAAGTATTGCAG550.11568474854342385No Hit
ACGATGTCACTGGTTTCATGAGAAGCTCTTTAGGAGTCGTTT530.11147803041457206No Hit
ACGATGTCGAACGTTACGCTCAGCTGAGTAAGCCATTCCAAC520.10937467135014617No Hit
ACGATGTCGTTATGCAAGGAAACCAAGTCCTGTGTTGTGTTT520.10937467135014617No Hit
ACGATGTCTAGGGTTTCGCTCGGGTCTGCGGGTTATAGCTTT510.1072713122857203No Hit
ACGATGTCACACGTTTTGCCGGCGCACAAGCACGATCGGCTC510.1072713122857203No Hit
ACGATGTCGCTCGTTTATTGGAAGCATCAACCTGCGCCGTCT500.10516795322129441No Hit
ACGATGTCGCCGATTTCCTACAACTGAAGCCAGAAAAGTGAT500.10516795322129441No Hit
ACGATGTTGGGAGTTGGGAGTTCGAGACCAGCCTGACCAACA480.10096123509244262No Hit
ACGATGTCGGGTGCTTCGCACCACACAGCCCTGGGGAATGCA480.10096123509244262No Hit
ACGATGTCTCTTGCTTGGTATAGAGTTGGATGTACTGACTCT480.10096123509244262No Hit
ACGATGTCTCTGATCGCGGACGATGTCTTTTTTTTTTTTTTT480.10096123509244262No Hit
ACGATGTCCTTAGTTTGGGAGGCCGAGGCAGGCGGATCACGA480.10096123509244262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGACG200.002069515636.06118416
CTAGGCG200.002069515636.06118418
TACGCTC200.002069515636.06118415
ATACGAC404.6426067E-836.06118414
ACGACTC404.6426067E-836.06118416
ACTAGGC200.002069515636.06118417
AAACGGA200.002069515636.06118414
AACGGAT200.002069515636.06118415
CGACTCA404.6426067E-836.06118417
TTTTCGT251.4143455E-436.0611813
TCAGAAC251.4143455E-436.0611818
GAATAGA309.714791E-636.0611818
AATACGA356.7030487E-736.0611813
AAAGCTA309.714791E-636.0611815
GTGGAGA251.4143455E-436.0611813
AAGCTAG309.714791E-636.0611816
GACTCAC309.714791E-636.0611818
TAAAGCT309.714791E-636.0611814
AGATATT251.4143455E-436.0611817
GTAAAGC356.7030487E-736.0611813
Produced by FastQC (version 0.11.5)