##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099231_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 47543 Sequences flagged as poor quality 0 Sequence length 42 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.83961887133753 37.0 35.0 37.0 35.0 37.0 2 36.170561386534295 37.0 37.0 37.0 35.0 37.0 3 36.05361462255222 37.0 37.0 37.0 35.0 37.0 4 33.8829480680647 35.0 33.0 35.0 31.0 37.0 5 37.00357571040952 39.0 37.0 39.0 34.0 39.0 6 37.63843257682519 39.0 37.0 39.0 35.0 39.0 7 37.245735439496876 39.0 37.0 39.0 34.0 39.0 8 37.8008749973708 39.0 39.0 39.0 35.0 39.0 9 32.13423637549166 33.0 31.0 34.0 31.0 34.0 10 32.4663988389458 34.0 31.0 34.0 31.0 34.0 11 32.62194224176009 34.0 31.0 34.0 31.0 34.0 12 35.984056538291654 37.0 37.0 37.0 35.0 37.0 13 31.140882990135246 31.0 30.0 31.0 30.0 34.0 14 30.650211387585976 31.0 30.0 31.0 27.0 34.0 15 30.08034831626107 31.0 30.0 31.0 26.0 34.0 16 33.135540458111606 35.0 32.0 37.0 28.0 37.0 17 34.16225311822981 35.0 33.0 37.0 30.0 37.0 18 32.36266116988831 35.0 30.0 36.0 25.0 37.0 19 31.74742864354374 35.0 30.0 35.0 23.0 37.0 20 31.819826262541277 35.0 30.0 35.0 23.0 37.0 21 33.43266095955241 35.0 31.0 39.0 25.0 39.0 22 33.965441810571484 35.0 32.0 39.0 27.0 39.0 23 34.89405380392487 37.0 33.0 39.0 28.0 39.0 24 35.547819868329725 37.0 34.0 39.0 30.0 39.0 25 35.83280398796879 37.0 35.0 39.0 31.0 39.0 26 37.31146541026018 38.0 36.0 40.0 32.0 41.0 27 37.58830953031992 39.0 36.0 40.0 33.0 41.0 28 37.87834171171361 39.0 37.0 40.0 33.0 41.0 29 38.10342216519782 39.0 37.0 40.0 34.0 41.0 30 38.28069326714763 40.0 37.0 40.0 35.0 41.0 31 38.53322255642261 40.0 38.0 40.0 35.0 41.0 32 38.5922428117704 40.0 38.0 40.0 36.0 41.0 33 38.630166375702 40.0 38.0 40.0 36.0 41.0 34 38.64244999263824 40.0 38.0 40.0 36.0 41.0 35 38.63161769345645 40.0 38.0 40.0 35.0 41.0 36 38.60843867656648 40.0 38.0 40.0 35.0 41.0 37 38.526596975369664 40.0 38.0 40.0 35.0 41.0 38 38.55011252970995 40.0 38.0 40.0 35.0 41.0 39 38.51042214416423 40.0 38.0 40.0 35.0 41.0 40 38.473613360536774 40.0 38.0 40.0 35.0 41.0 41 38.493006331110784 40.0 38.0 40.0 35.0 41.0 42 38.51096901752098 40.0 38.0 40.0 35.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.494654674627526 1101 2 0.44928684721892864 1101 3 0.5645738470429009 1101 4 1.0046965380881971 1101 5 0.8202130157215777 1101 6 0.850166925860961 1101 7 0.44355013307689717 1101 8 0.6821401869414743 1101 9 0.5141044423552543 1101 10 0.7612134789495144 1101 11 0.36752944176336655 1101 12 0.5537687524561008 1101 13 -0.1854428386277469 1101 14 -0.11173952912573526 1101 15 -0.13995294130391756 1101 16 -0.9999985499692556 1101 17 -0.45231653675153183 1101 18 -1.5213125971150845 1101 19 0.5647382117903597 1101 20 0.5372561897665022 1101 21 -0.255986008798736 1101 22 0.5367018955086564 1101 23 0.25247264187601814 1101 24 0.2017937189318033 1101 25 -0.22258257030134843 1101 26 -0.09314010179231502 1101 27 -0.22852910885025324 1101 28 -0.2419045118619607 1101 29 0.09464882080737169 1101 30 0.05053496546796765 1101 31 0.04422175669097328 1101 32 -0.29635431967690806 1101 33 -0.41695402210456933 1101 34 -0.15697817271033898 1101 35 0.36068173840289575 1101 36 0.39741607836046455 1101 37 0.44865485863304144 1101 38 0.2698518022177012 1101 39 0.9480326185992496 1101 40 0.5184584402663361 1101 41 -0.6625505273949983 1101 42 -0.3984991648652212 1102 1 -0.12049310862370533 1102 2 -0.15847177347072972 1102 3 -0.05919462586350477 1102 4 -0.045180309202443425 1102 5 -0.1058953586626572 1102 6 -0.565473468227708 1102 7 -0.3563267142137434 1102 8 -0.6454460199550809 1102 9 -0.16385122266937557 1102 10 0.2544400799347386 1102 11 0.05656885063035588 1102 12 0.2766751563969905 1102 13 -0.2888911144898145 1102 14 -0.1653109576971623 1102 15 0.2067219355433707 1102 16 0.564656622444538 1102 17 -0.44246432000276315 1102 18 -0.7226672769180418 1102 19 -1.1033652364855016 1102 20 -0.630847258509359 1102 21 -1.969040195990857 1102 22 -2.2748744591711443 1102 23 -1.6859510034441811 1102 24 -0.6362604682603177 1102 25 -0.7780012895131705 1102 26 -0.7384602988366495 1102 27 -0.6220020152541963 1102 28 0.02533686744838093 1102 29 -0.6054743319019877 1102 30 -0.03444040399016046 1102 31 -0.09925114971296978 1102 32 -0.6725858467705024 1102 33 -0.4612889974740284 1102 34 -0.05537718748866638 1102 35 -0.7279882123360153 1102 36 -0.5724115078464322 1102 37 -0.43311854038173436 1102 38 -0.36623194162466177 1102 39 0.08473212598840973 1102 40 0.0492466176062436 1102 41 0.14841006373801235 1102 42 0.09534319966679305 1103 1 -0.004051329513259816 1103 2 -0.030785616549188433 1103 3 -0.15514664985150972 1103 4 0.333630285500206 1103 5 -0.3122921602618547 1103 6 0.13717520743445988 1103 7 -0.49925524994587533 1103 8 0.1676474333182867 1103 9 0.31715827258300067 1103 10 0.21695882056442173 1103 11 0.059817226442454796 1103 12 0.1919675102200742 1103 13 -0.17095008500455933 1103 14 -0.14357182726238094 1103 15 5.750090066385383E-4 1103 16 0.005436232639439709 1103 17 -0.4274718162546378 1103 18 -0.44755483313992883 1103 19 -1.1725806287893512 1103 20 -0.6058597522624822 1103 21 -1.3041226547614713 1103 22 -1.2164036945534562 1103 23 -1.5994942318299863 1103 24 -1.109024086451221 1103 25 -0.9456674564297103 1103 26 -1.032063497237452 1103 27 -0.30591006123120223 1103 28 -0.658571178528625 1103 29 -0.49877768022782476 1103 30 -0.32679422707861505 1103 31 -0.23968093482041297 1103 32 -0.12735846046365396 1103 33 0.21037517044201337 1103 34 -0.2033032244701758 1103 35 0.2457749061047636 1103 36 0.37386535372278473 1103 37 0.4062117944508472 1103 38 0.22197395542681164 1103 39 0.12246326042119904 1103 40 -0.2263655762968071 1103 41 -0.2968672975813291 1103 42 -0.069824216625058 1104 1 -0.0799251573052473 1104 2 -0.08747785866342639 1104 3 0.2105822504042436 1104 4 0.16120914313021473 1104 5 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1104 41 0.41413014487391564 1104 42 0.3935176418574642 1105 1 0.4678689603418107 1105 2 0.21491184721892864 1105 3 0.14716313275718562 1105 4 0.13639296665962775 1105 5 -0.02130484142127642 1105 6 -0.09402950271046961 1105 7 0.34087156164832777 1105 8 0.21339018694147427 1105 9 0.24401515664096962 1105 10 0.142909907520945 1105 11 0.5215472989062206 1105 12 0.1899294667418161 1105 13 0.2676821613722531 1105 14 0.4753140423028377 1105 15 0.1636184872675095 1105 16 0.20312645003074437 1105 17 -0.02597725103724713 1105 18 1.0925266885992002 1105 19 0.39286321179035966 1105 20 0.8028811897665022 1105 21 0.5654425626298334 1105 22 0.5634876097943717 1105 23 0.44443692759030284 1105 24 0.6638472903603727 1105 25 0.5765245725557975 1105 26 0.4738241839219697 1105 27 0.25138160543546206 1105 28 -0.11690451186196071 1105 29 0.3848273922359411 1105 30 0.46124925118225235 1105 31 -0.10086752902331142 1105 32 0.5585563946088072 1105 33 0.656706692181146 1105 34 0.24257539871823042 1105 35 0.13300316697432635 1105 36 0.5849160783604646 1105 37 0.26785128720447204 1105 38 0.5890482307891318 1105 39 0.4435683328849649 1105 40 0.14569058312348204 1105 41 0.2459316154621476 1105 42 0.6416794065633482 1106 1 0.24767665264950267 1106 2 0.19351761644969656 1106 3 0.052211209680265824 1106 4 0.35057565896732257 1106 5 0.30153169704026084 1106 6 0.32736472805876105 1106 7 0.20866002318678767 1106 8 -0.32555212075083517 1106 9 0.007236310487122921 1106 10 -0.14290740017136017 1106 11 0.011210760444683388 1106 12 -0.24348399479664806 1106 13 -0.1854428386277469 1106 14 -0.26531095769716373 1106 15 -0.5171507435017197 1106 16 -0.7096139345846382 1106 17 -0.7819868664218603 1106 18 -0.2529060037084889 1106 19 -0.5487233266711797 1106 20 0.4391792666895782 1106 21 -0.31148051429324397 1106 22 -0.09132008251332024 1106 23 0.17664846605184437 1106 24 -0.6479315557934768 1106 25 -0.3835715812903544 1106 26 -0.9381950468472624 1106 27 -0.40847416379530443 1106 28 0.20809548813804213 1106 29 0.12953893069747835 1106 30 -0.5214430565100585 1106 31 -0.1703386828694633 1106 32 0.6160083176857327 1106 33 0.4973316921811488 1106 34 0.4432965525643837 1106 35 0.15079162851278483 1106 36 -0.2814300754856873 1106 37 0.06953397951216544 1106 38 -0.5688844615185573 1106 39 -0.34152782096118983 1106 40 0.0574694292773259 1106 41 0.4168450770006089 1106 42 -0.03860905497511169 1107 1 -0.6058250695089384 1107 2 -0.08616091397509962 1107 3 -0.024711867242814378 1107 4 0.043342593525302675 1107 5 0.30635374066827836 1107 6 0.2368940047522159 1107 7 -0.29765455775465455 1107 8 0.07766257500117746 1107 9 -0.027206858284401392 1107 10 -0.24118524173278644 1107 11 -0.24734262646691718 1107 12 -0.056572398929823464 1107 13 -0.006338361015806981 1107 14 -0.14292289799567115 1107 15 0.06287221861079217 1107 16 -0.09514780370059839 1107 17 -0.16660225103724713 1107 18 -0.256120699460503 1107 19 -1.1535453702991916 1107 20 -1.121325899785738 1107 21 -1.0464977358776295 1107 22 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16 -0.3304780020240514 1108 17 -0.5638625250098528 1108 18 1.2969359351745453 1108 19 0.641793006310909 1108 20 -0.39938764584993436 1108 21 -0.16058483463044126 1108 22 0.43934377417793513 1108 23 0.14777596868619014 1108 24 0.4119209204973586 1108 25 0.74561703830922 1108 26 0.8432591154288218 1108 27 0.8402086602299832 1108 28 0.43275302238461677 1108 29 0.5582006799071735 1108 30 0.4355643196754002 1108 31 0.4246033613876463 1108 32 0.19704098364990585 1108 33 0.2288385414962164 1108 34 0.2620531384442586 1108 35 -0.10800710699827931 1108 36 0.39570374959333776 1108 37 0.28133587624557066 1108 38 -0.1560288240053893 1108 39 0.0972498397342818 1108 40 0.23323339134265808 1108 41 0.20462168395529545 1108 42 0.5188198175222496 1109 1 0.033747338720189646 1109 2 0.06753009046217073 1109 3 0.0023151597842101523 1109 4 0.017727426119087397 1109 5 0.08469178020034462 1109 6 0.43173063242466725 1109 7 0.4498242643510295 1109 8 0.060518565319853224 1109 9 0.056092859343671364 1109 10 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0.1746379649286851 2212 22 -0.2059089419297635 2212 23 0.29106049080869667 2212 24 0.018911945532785523 2212 25 0.02084928519947482 2212 26 0.543148896565647 2212 27 -0.32889856697833153 2212 28 -0.3022493394481671 2212 29 -0.3008766307525619 2212 30 0.4253297109523686 2212 31 0.01569137902266249 2212 32 0.24695438311455575 2212 33 -0.04749589402575083 2212 34 0.12853085848834667 2212 35 0.4501727072042101 2212 36 0.19195630824552268 2212 37 0.29676651708953017 2212 38 0.26595196642131924 2212 39 0.3433528156435841 2212 40 -0.4277648766466342 2212 41 0.02197328212881189 2212 42 0.1585615904713933 2213 1 0.10658847253693438 2213 2 0.06818928624331733 2213 3 -0.0734923550476907 2213 4 -0.05148813090134752 2213 5 0.10941162199335963 2213 6 0.10147354607001802 2213 7 0.10078009823369172 2213 8 0.1577499430390361 2213 9 0.12168283956779646 2213 10 0.16234588313069764 2213 11 0.19113571354036907 2213 12 0.07903007649791505 2213 13 0.046264478445422696 2213 14 0.22493294474186243 2213 15 0.15752092629189818 2213 16 -0.4908522085058422 2213 17 0.2968123831090921 2213 18 0.7979382739650518 2213 19 -0.17611544674622692 2213 20 0.8329878970835765 2213 21 -0.41016719346772845 2213 22 0.17171931711144595 2213 23 -0.10815453582433321 2213 24 0.5201735098725706 2213 25 0.49954286523872327 2213 26 0.5340375985561181 2213 27 -0.017292175052340042 2213 28 0.1788271954551135 2213 29 0.35205300199204004 2213 30 -0.04789709028116107 2213 31 0.005458690488886475 2213 32 0.03683383363319592 2213 33 -0.08071708830665614 2213 34 0.13091381335237884 2213 35 0.09413121575481398 2213 36 -0.020266848468800447 2213 37 0.07234823842398441 2213 38 0.09171591371595866 2213 39 0.13183052800691542 2213 40 -0.1496600266326169 2213 41 -0.1812787503915132 2213 42 -0.06487547148542916 2214 1 -0.11207290012330873 2214 2 -0.19896896673456155 2214 3 -0.3154095416614169 2214 4 0.2908261061945083 2214 5 -0.25786007397941546 2214 6 -0.26154403759419154 2214 7 -0.6831109964912088 2214 8 -0.4922784177096915 2214 9 -0.7512610061497291 2214 10 -0.3654476506185915 2214 11 -0.3480021196984282 2214 12 0.006426559765074558 2214 13 0.10525483579085915 2214 14 0.032363460907490094 2214 15 0.06478127796518507 2214 16 0.5494200546819101 2214 17 -0.8177650417349227 2214 18 -0.7094355207031242 2214 19 -0.31026178820964034 2214 20 0.336674794417668 2214 21 0.7282332603042505 2214 22 0.5707550516548352 2214 23 0.0265590206135613 2214 24 0.5493850810580483 2214 25 -0.027399846048851373 2214 26 0.2739695327591818 2214 27 0.7328496286912767 2214 28 0.41507223232408563 2214 29 0.2910773922359411 2214 30 0.24903994885666947 2214 31 -0.11649252902331142 2214 32 -0.09078953562374892 2214 33 -0.41197063340025153 2214 34 -0.30357285709572324 2214 35 0.1729740972068825 2214 36 0.14450910161627917 2214 37 0.2609472174370282 2214 38 -0.1300651413038949 2214 39 0.40069042590822335 2214 40 0.4229434901002236 2214 41 0.47158568522959143 2214 42 0.2601387088889311 2215 1 -0.3061695011966492 2215 2 -0.11161058867850926 2215 3 -0.21701955955050778 2215 4 -0.15968075128908765 2215 5 -0.3830836875751231 2215 6 -0.3444301437361119 2215 7 -0.13749382296705903 2215 8 -0.21529571049442353 2215 9 -0.14660984335903038 2215 10 -0.8582920155559783 2215 11 -0.16314821391429035 2215 12 0.1590801077674584 2215 13 0.01968536650045749 2215 14 -0.3704391628253667 2215 15 0.47772105137007514 2215 16 0.23397580900510206 2215 17 0.48724390280890617 2215 18 -0.2452136960161866 2215 19 -0.015389993337844743 2215 20 0.7519997795100934 2215 21 -0.152506155318882 2215 22 0.44970555851232064 2215 23 0.19203308143645614 2215 24 0.2443761365142194 2215 25 -0.4194690171877937 2215 26 -0.7817847904370012 2215 27 -0.6136023689235088 2215 28 -0.6970327169901651 2215 29 -0.5935379923794457 2215 30 -0.38810972317671855 2215 31 -0.09085150338228232 2215 32 0.062162163839573736 2215 33 0.18194707679653277 2215 34 0.03304014230797492 2215 35 0.21233009005124615 2215 36 -0.27886597292158655 2215 37 -0.05867114869296586 2215 38 0.02855143591733622 2215 39 0.2969337175003517 2215 40 0.11644378825168644 2215 41 -0.06777030761477221 2215 42 0.2434422270761658 2216 1 0.0747237990514904 2216 2 -0.04668089471655179 2216 3 -0.411808641436366 2216 4 0.12278409569188398 2216 5 -0.6337040349696608 2216 6 -0.18727547045240556 2216 7 -0.027071986738768317 2216 8 -0.0597952969294937 2216 9 -0.4046743594880624 2216 10 -0.33844089893067064 2216 11 -0.21484383012603558 2216 12 -0.0948487590646323 2216 13 0.8952023226625734 2216 14 0.8992051713350975 2216 15 1.2039410679126696 2216 16 1.1653240306759045 2216 17 1.2204945231563045 2216 18 2.365208140212104 2216 19 1.3994156311451995 2216 20 1.1461271575084382 2216 21 2.210603852952417 2216 22 2.0332456743105 2216 23 1.9897998308161107 2216 24 1.236931967779725 2216 25 0.7831777983622459 2216 26 0.6603161194058416 2216 27 0.844627573177398 2216 28 0.5080954881380393 2216 29 0.5652709406230372 2216 30 0.7152815092467719 2216 31 0.738346180654105 2216 32 0.08325397525396738 2216 33 0.07152524056824205 2216 34 0.3055794309762945 2216 35 0.45054348955497403 2216 36 0.5627386590056247 2216 37 0.7159359646238244 2216 38 -0.2733509627592525 2216 39 0.2510280103043172 2216 40 0.3145413895750977 2216 41 0.5488550025589234 2216 42 0.5197036001117326 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 5.0 26 11.0 27 46.0 28 155.0 29 293.0 30 509.0 31 945.0 32 1672.0 33 3093.0 34 5988.0 35 9660.0 36 11216.0 37 8773.0 38 5177.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.6478345918431735 98.85997938708117 0.16826872515407104 0.32391729592158675 2 0.27343667837536545 0.601560692425804 0.18930231579832993 98.9357003134005 3 98.40775718822961 0.618387564941211 0.5216330479776202 0.452222198851566 4 0.2692299602465137 98.55288896367499 0.41646509475632587 0.7614159813221716 5 0.5637002292661379 0.7845529310308562 97.91767452621836 0.734072313484635 6 98.5023768457364 0.7908796432627991 0.35968196542004965 0.34706154558074964 7 1.1821126582810988 0.9023600185099491 97.20037019898196 0.7151571242269993 8 0.8118965988683928 0.6120774877479335 1.2094314620448858 97.36659445133878 9 32.681993143049446 15.238836421765559 28.975874471531032 23.103295963653956 10 22.888753339082516 22.703657741413036 19.977704393917087 34.42988452558736 11 33.012200252419014 13.273033235170384 29.27219183845183 24.442574673958774 12 21.431576290437928 24.378444322914476 17.94834041479113 36.24163897185647 13 60.07068624563471 23.038246307905922 7.87857112803467 9.012496318424706 14 19.023179504438183 22.49589836355223 47.707290395860504 10.773631736149088 15 15.508066382012073 18.223502934185895 54.14256567738679 12.125865006415246 16 16.019182634667565 23.252634457228194 49.361630523946744 11.3665523841575 17 15.726815724712365 26.542288033990282 12.689565235681382 45.04133100561597 18 45.8270202020202 25.237794612794612 13.587962962962962 15.347222222222223 19 25.8356296935148 29.46212583352616 23.115757588506277 21.586486884452764 20 20.627642344824686 27.619207874976336 28.01043266095955 23.742717119239426 21 24.07925456954757 26.245714405906234 25.97648444565972 23.698546578886482 22 25.2760658772059 27.246913320572958 28.20604505395116 19.270975748269986 23 22.87709555962222 28.200921310027134 26.869439010538272 22.052544119812374 24 23.128536272427066 26.277264791872625 27.066024441032326 23.528174494667986 25 22.00113581389479 28.193424899564608 27.171192394253623 22.63424689228698 26 22.39446395894243 30.284163809603935 25.60629325032076 21.71507898113287 27 24.952148581284312 26.695833245693372 28.04618976505479 20.305828407967525 28 24.243316576572788 29.57743516395684 25.35178680352523 20.827461455945144 29 24.838567191805314 28.29228277559262 27.09967818606314 19.76947184653892 30 21.773973034936795 29.12310960604085 27.2258797299287 21.877037629093664 31 23.4398334139621 28.374313779105233 26.3487790000631 21.83707380686957 32 22.40498075426456 30.0780346212902 25.08045348421429 22.436531140230947 33 22.649446804930378 28.290774473097475 28.231879180514074 20.827899541458077 34 23.171090824954778 29.47078372807202 27.211728576837324 20.14639687013588 35 22.39446395894243 29.234587636455416 27.58555412994552 20.78539427465663 36 22.217781797530655 28.170287949855922 27.917884862124815 21.69404539048861 37 22.221988515659508 28.429001114780306 27.728582546326486 21.620427823233705 38 22.463874808068486 27.86319752644974 27.74751277790632 21.92541488757546 39 22.514355425614706 28.601476558063226 27.575037334623392 21.309130681698672 40 19.615926634835834 29.72467029846665 29.125212965105273 21.534190101592245 41 20.316345203289654 29.712050144080095 27.87792103990072 22.093683612729528 42 21.92166926086408 28.294981279710573 28.107778385427622 21.67557107399773 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 2.0 8 4.5 9 7.0 10 7.0 11 14.5 12 22.0 13 29.5 14 37.0 15 63.0 16 89.0 17 89.0 18 118.0 19 147.0 20 192.5 21 238.0 22 238.0 23 317.5 24 397.0 25 523.5 26 650.0 27 801.0 28 952.0 29 952.0 30 1243.0 31 1534.0 32 1780.5 33 2027.0 34 2027.0 35 2209.0 36 2391.0 37 2535.0 38 2679.0 39 2930.0 40 3181.0 41 3181.0 42 3307.5 43 3434.0 44 3981.5 45 4529.0 46 4249.5 47 3970.0 48 3970.0 49 3996.5 50 4023.0 51 4077.0 52 4131.0 53 4131.0 54 3918.0 55 3705.0 56 3544.0 57 3383.0 58 2826.0 59 2269.0 60 2269.0 61 1848.5 62 1428.0 63 1161.5 64 895.0 65 747.5 66 600.0 67 600.0 68 480.0 69 360.0 70 246.5 71 133.0 72 133.0 73 122.0 74 111.0 75 120.0 76 129.0 77 85.5 78 42.0 79 42.0 80 38.5 81 35.0 82 21.5 83 8.0 84 8.0 85 6.5 86 5.0 87 2.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.002103359064425888 7 0.002103359064425888 8 0.0 9 0.0 10 0.0 11 0.006310077193277664 12 0.002103359064425888 13 0.018930231579832993 14 0.002103359064425888 15 0.0 16 0.0 17 0.0 18 0.04837725848179542 19 0.008413436257703553 20 0.0 21 0.0 22 0.0 23 0.004206718128851776 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.002103359064425888 34 0.002103359064425888 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.002103359064425888 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 47543.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.771470037650126 #Duplication Level Percentage of deduplicated Percentage of total 1 76.37480798771121 41.831605073302065 2 11.74347158218126 12.864144038028732 3 4.308755760368664 7.079906610857539 4 2.334869431643625 5.115369244683761 5 1.4362519201228878 3.9332814504764104 6 0.8179723502304148 2.6880928843362852 7 0.6067588325652842 2.3263151252550323 8 0.41090629800307216 1.8004753591485603 9 0.3225806451612903 1.5901394527059716 >10 1.5207373271889402 14.637275729339756 >50 0.08832565284178187 3.2349662410870157 >100 0.03456221198156682 2.898428790778874 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACGATGTCGTCCGTAAAGCTAGGGGCTGGTGAGATGGCTCAG 259 0.544769997686305 No Hit ACGATGTCATGCGTTTCAGTCTCGACGGGCTAGCACACATCT 248 0.5216330479776202 No Hit ACGATGTCTCTTGTTTAGTAGTTCCTGATCCCAGCTCTTTCC 169 0.3554676818879751 No Hit ACGATGTCCTGCGTGGACTCAAGCAGTCTCTCAACCCACAGG 152 0.31971057779273504 No Hit ACGATGTCGCACGTTTCAGTCTCGACGGGCTAGCACACATCT 125 0.262919883053236 No Hit ACGATGTCTGAGGATTGGAATAGAGACGTACCCTTAGCACAC 111 0.23347285615127358 No Hit ACGATGTCCATAGAAACGGATTGCAAGCAATCAGCACTGTAG 108 0.22716277895799591 No Hit ACGATGTCTGTGGTTTCTCAGAACGCTGCTGTGTGCTTTTTA 105 0.22085270176471825 No Hit ACGATGTCGCCCGGATAGTCTTGGGCGCTAACATTTTACAAC 101 0.21243926550701472 No Hit ACGATGTCAGTAGTTTTGCCGGCGCACAAGCACGATCGGCTC 99 0.20823254737816294 No Hit ACGATGTCTCAAGTTTTGCCGGCGCACAAGCACGATCGGCTC 92 0.1935090339271817 No Hit ACGATGTCTCTGGTAAGGACCGCCGCTTTCGCTCGGGTCTGC 86 0.18088887954062638 No Hit ACGATGTCTCCCGTATCGCGCACGTAGTAGCCTAGAGCGCCA 78 0.16406200702521928 No Hit ACGATGTCACAGGGGAAGAGGAGGCAGAAAGATTGTAGAAAC 77 0.16195864796079337 No Hit ACGATGTCCACCGTTTACTTTGAAAAAATTAGAGTGTTCAAA 76 0.15985528889636752 No Hit ACGATGTTTCGAGAAACGTGAAAAATGAGAAATGCACACTGA 75 0.1577519298319416 No Hit ACGATGTAGTGCATTTCTACCATACCCTACACCACACTTTGC 75 0.1577519298319416 No Hit ACGATGTCGCTGGAAAAGTTTTGCCGGCGCACAAGCACGATC 72 0.15144185263866394 No Hit ACGATGTCCGAGGTTTCAGTCTCGACGGGCTAGCACACATCT 70 0.14723513450981218 No Hit ACGATGTCAAGTGAATAGGAGTTTTCCTGTGCTTCCCTTAAA 67 0.14092505731653449 No Hit ACGATGTCTCCCGTCTAACAACAACATCCACACTACTACTCC 63 0.13251162105883094 No Hit ACGATGTCTCTTGTTTCAATAAAAGAAAAATAGAAGGGAAAG 62 0.13040826199440506 No Hit ACGATGTCCAGCGAATAATGGAATAGGACCGCGGTTCTATTT 59 0.1240981848011274 No Hit ACGATGTCGTTTGTTTCAGTCTCGACGGGCTAGCACACATCT 59 0.1240981848011274 No Hit ACGATGTCGGGTAATACGACTCACTGTAGAACTCTGAACCCC 57 0.11989146667227561 No Hit ACGATGTCGGGCATTTTGAGGTCTTAACTTCTTTCTTTTTAA 57 0.11989146667227561 No Hit ACGATGTCAGGAGATCCAAGTGGGCCACATGAAGTATTGCAG 55 0.11568474854342385 No Hit ACGATGTCACTGGTTTCATGAGAAGCTCTTTAGGAGTCGTTT 53 0.11147803041457206 No Hit ACGATGTCGAACGTTACGCTCAGCTGAGTAAGCCATTCCAAC 52 0.10937467135014617 No Hit ACGATGTCGTTATGCAAGGAAACCAAGTCCTGTGTTGTGTTT 52 0.10937467135014617 No Hit ACGATGTCTAGGGTTTCGCTCGGGTCTGCGGGTTATAGCTTT 51 0.1072713122857203 No Hit ACGATGTCACACGTTTTGCCGGCGCACAAGCACGATCGGCTC 51 0.1072713122857203 No Hit ACGATGTCGCTCGTTTATTGGAAGCATCAACCTGCGCCGTCT 50 0.10516795322129441 No Hit ACGATGTCGCCGATTTCCTACAACTGAAGCCAGAAAAGTGAT 50 0.10516795322129441 No Hit ACGATGTTGGGAGTTGGGAGTTCGAGACCAGCCTGACCAACA 48 0.10096123509244262 No Hit ACGATGTCGGGTGCTTCGCACCACACAGCCCTGGGGAATGCA 48 0.10096123509244262 No Hit ACGATGTCTCTTGCTTGGTATAGAGTTGGATGTACTGACTCT 48 0.10096123509244262 No Hit ACGATGTCTCTGATCGCGGACGATGTCTTTTTTTTTTTTTTT 48 0.10096123509244262 No Hit ACGATGTCCTTAGTTTGGGAGGCCGAGGCAGGCGGATCACGA 48 0.10096123509244262 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.004206718128851776 0.0 0.0 14 0.0 0.0 0.01262015438655533 0.0 0.0 15 0.0 0.0 0.01262015438655533 0.0 0.0 16 0.0 0.0 0.03996382222409187 0.0 0.0 17 0.0 0.0 0.03996382222409187 0.0 0.0 18 0.0 0.0 0.06099741286835075 0.0 0.0 19 0.0 0.0 0.06310077193277665 0.0 0.0 20 0.0 0.0 0.07572092631933197 0.0 0.0 21 0.0 0.0 0.1787855204762005 0.0 0.0 22 0.0 0.0 0.23347285615127358 0.0 0.0 23 0.0 0.0 0.24188629240897713 0.0 0.0 24 0.0 0.0 0.2776433965042172 0.0 0.0 25 0.0 0.0 0.32181393685716086 0.0 0.0 26 0.0 0.0 0.3491576046946974 0.0 0.0 27 0.0 0.0 0.37019119533895634 0.0 0.0 28 0.0 0.0 0.45222219885156595 0.0 0.0 29 0.0 0.0 0.4648423532381213 0.0 0.0 30 0.0 0.0 0.4942893801400837 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGGACG 20 0.0020695156 36.061184 16 CTAGGCG 20 0.0020695156 36.061184 18 TACGCTC 20 0.0020695156 36.061184 15 ATACGAC 40 4.6426067E-8 36.061184 14 ACGACTC 40 4.6426067E-8 36.061184 16 ACTAGGC 20 0.0020695156 36.061184 17 AAACGGA 20 0.0020695156 36.061184 14 AACGGAT 20 0.0020695156 36.061184 15 CGACTCA 40 4.6426067E-8 36.061184 17 TTTTCGT 25 1.4143455E-4 36.06118 13 TCAGAAC 25 1.4143455E-4 36.06118 18 GAATAGA 30 9.714791E-6 36.06118 18 AATACGA 35 6.7030487E-7 36.06118 13 AAAGCTA 30 9.714791E-6 36.06118 15 GTGGAGA 25 1.4143455E-4 36.06118 13 AAGCTAG 30 9.714791E-6 36.06118 16 GACTCAC 30 9.714791E-6 36.06118 18 TAAAGCT 30 9.714791E-6 36.06118 14 AGATATT 25 1.4143455E-4 36.06118 17 GTAAAGC 35 6.7030487E-7 36.06118 13 >>END_MODULE