FastQCFastQC Report
Thu 26 May 2016
SRR2099229_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099229_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71076
Sequences flagged as poor quality0
Sequence length42
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCTCCGTCTTTTAAATCCTGGTTTGACAAGCTACTTCTT2370.33344588890764815No Hit
GATGCTCATCGCGTAAAATGGTGAAGCTTATGAATTTGTGGA1560.219483369913895No Hit
GATGCTCAGCAGGCTAAGGAATTGGTGAAGTAAAATGGTGAA1140.16039169339861556No Hit
GATGCTCAGACAGTGTGATTCTCGAGCGATTTATACATCCGT1020.14350835725139288No Hit
GATGCTCAATTAGGGAAGGGATCACATTATGGTCAGTGCGAA1000.1406944678935224No Hit
GATGCTCTTTACTATGCCCCTGTACAGGGGAACGTCAGGGCC960.13506668917778153No Hit
GATGCTCAAGGGGGAGCGGACTTGTAAGGACCGCCGCTTTCG890.12521807642523497No Hit
GATGCTCACTAAGTGAATGACGCATGAGCCTCGTATCGAGTG880.12381113174629974No Hit
GATGCTCAGGCGGTTGACTAGGCGCATAGTCGCCATTCACAG870.12240418706736451No Hit
GATGCTCATAAGGATTGGAGCTGTTTAGGAAGCAATAGGCAT790.1111486296358827No Hit
GATGCTTACGCCAATTAGTCCGACATGGTGGTGCATGCCTGT780.1097416849569475No Hit
GATGCTCAACAGGCATAGTCGCCATTCACAGATTTGCTCGGC740.10411390624120659No Hit
GATGCTCAGAACGGTTGGGTAAGATGAAATACAATGCAATAA740.10411390624120659No Hit
GATGCGCCAGGGGGTGGAATCAGTGGATGCTGCAAGGAAGTC720.10130001688333613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCG309.821164E-636.0408747
CATGCCG356.7960536E-736.0408747
CATTGCG309.821164E-636.0408747
CACGCGA251.4261845E-436.0408747
CATCTAG356.7960536E-736.0408747
CACTCCG309.821164E-636.0408747
CATAGCG309.821164E-636.0408747
CAAGCGG251.4261845E-436.0408747
CACGTGC251.4261845E-436.0408747
CAGGTCG200.00208221136.040877
CACGGCA200.00208221136.040877
CATGCGG200.00208221136.040877
CAATTGA200.00208221136.040877
CACCTTG200.00208221136.040877
CACGTCA200.00208221136.040877
CATCCTT200.00208221136.040877
CAATCCG200.00208221136.040877
CAATACG200.00208221136.040877
CACCACG200.00208221136.040877
CAGACCG200.00208221136.040877
Produced by FastQC (version 0.11.5)