FastQCFastQC Report
Thu 26 May 2016
SRR2099227_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099227_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35686
Sequences flagged as poor quality0
Sequence length42
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTACAGCTGTAGTTCGAGGAGGAGGAGGGTGAAGATGAGG4331.213360981897663No Hit
TGCTACAGGTGGGTTTCACTAGATCGTGTTATGGACGATGAC1240.34747520035868407No Hit
TGCTACAGCATAGTTGCAGTGAGCCGAGATCATGCCACTGCA1230.3446729810009528No Hit
TGCTACAGGCGATTCGAAGCGGGATCTCGCCCACCATTTGGG1140.31945300678137084No Hit
TGCTACAGCCCTGTTTGGTTTGCATGACTCTTTCCTCAATGA1030.2886285938463263No Hit
TGCTACAGCTGAGTTTACTTTGAAAAAATTAGAGTGTTCAAA920.2578041809112817No Hit
TGCAACCGAGCGGCGGCGCGCCGGTGGGCGCGGACCGTCGGG890.24939752283808778No Hit
TGCTTCAACTCCAGAAGCAGGCTCCATGCACCAGGAGCCCGA870.2437930841226251No Hit
TGCTACAGTGCTGGTTCGATCAGGCTACTACAAAGTTCTGGG810.2269797679762372No Hit
TTCGACAGTGGTGTTTTGGGCAAAATTGAGAGAGCCATCGGC780.2185731099030432No Hit
TGCTACAGTGGCGATTAGTTTAATTAAACTAACGAATAGTTT730.2045620131143866No Hit
TGCTACAGCAAAGCTTCGGAGGTCGTGGGTGACAAGTTGGTG680.19055091632572996No Hit
TGCTACAGGGGAGTTGCAGAAAAGTCATTGCATGGAAAGATG660.18494647761026733No Hit
TGCTACAGCATAGTTTACTTTGAAAAAATTAGAGTGTTCAAA650.18214425825253602No Hit
TGCTACAGATCGGTTTCAGTCTCGACGGGCTAGCACACATCT550.15412206467522277No Hit
TGCTACAGGGGCGATTCCCCATGGCTAAGATCTCACACTGCT540.15131984531749146No Hit
TGCTACAGGGGTGGAGCGGACTTGTAAGGACCGCCGCTTTCG530.14851762595976015No Hit
TGCTACAGTGTAGCGACGATCGGACTGTAGAACTCTGAACCC500.14011096788656616Illumina DpnII expression Adapter 1 (95% over 23bp)
TGCTACAGTGCTGGGGCGCATTGCGATCCAACCATCGGCAGC500.14011096788656616No Hit
TGCTACAGGTGCGTTTACTTTGAAAAAATTAGAGTGTTCAAA490.13730874852883485No Hit
TGCTACAGTTTGGTTTACTTTGAAAAAATTAGAGTGTTCAAA480.13450652917110353No Hit
TGGTCCAGGGCGGCATCTGGATACCGGCGTACCTGGAGCACG470.1317043098133722No Hit
TGCTACAGCTGGAATTCTTGGACCGGCGCAAGACGGACCAGA450.12609987109790954No Hit
TGCAGCAGTGAGGGGCAGGTGCGCAACTTCTCCATTCCGGGC440.12329765174017822No Hit
TGCTACAGAGCTGTGAATTAGATAGGGAAAAGATCGGGATGC420.11769321302471558No Hit
TGCTACAGTAGGGTTTGGCAAATCTGTCCACCAGAATTGCTC420.11769321302471558No Hit
TGCTACAGATCGAATTTGACATGAAAATCAAAGGCTAGGGCT420.11769321302471558No Hit
GGCGACAGGCATGGCCGATGGCCGCACGCTGTGGCTGCTGTC410.11489099366698424No Hit
TGCTACAGCAGTGTTTACTTTGAAAAAATTAGAGTGTTCAAA400.11208877430925292No Hit
TGCTACAGATGCGTTTCCCCTGTTCTTACCCTATATAAGTTG380.10648433559379028No Hit
TGCTACAGTGAGGTTTACTTTGAAAAAATTAGAGTGTTCAAA380.10648433559379028No Hit
TGCTACAGCCCACGACGATCTACTTGCTACAGTTTTTTTTTT370.10368211623605897No Hit
TGCTACAGTGACAGTTCTACAGTCCGACGATCGTCGGACGTA360.10087989687832764No Hit
TGCTACAGCATAAATTAGCTGCCACCAGGCCCAGCTAATTTT360.10087989687832764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGC8150.036.03
CGACAGA950.036.03
CAGCTAT251.4214279E-436.06
ACAGCCA309.760084E-636.05
ACAGTCT601.8189894E-1236.05
GATGGCC251.4214279E-436.017
ACAGCAA251.4214279E-436.05
ACAGTAT251.4214279E-436.05
ACGCTGT251.4214279E-436.026
CAGCATT251.4214279E-436.06
CAGCATA251.4214279E-436.06
CAGTCCA309.760084E-636.06
ATCTCGC251.4214279E-436.024
ACAGATG453.2305252E-936.05
ACAGATC502.237357E-1036.05
ACAGAGT551.6370905E-1136.05
ACAGAGC700.036.05
TACAGGT2450.036.04
TACAGGC1800.036.04
TTACTTT850.036.015
Produced by FastQC (version 0.11.5)