##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099227_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35686 Sequences flagged as poor quality 0 Sequence length 42 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.70215210446674 37.0 35.0 37.0 35.0 37.0 2 35.907778960937065 37.0 37.0 37.0 35.0 37.0 3 36.152973154738554 37.0 37.0 37.0 35.0 37.0 4 36.15232864428627 37.0 37.0 37.0 35.0 37.0 5 36.93944403967943 38.0 37.0 39.0 34.0 39.0 6 37.967830521773244 39.0 39.0 39.0 37.0 39.0 7 37.152356666479854 39.0 37.0 39.0 34.0 39.0 8 37.48049655327019 39.0 37.0 39.0 35.0 39.0 9 32.217928599450765 33.0 31.0 34.0 31.0 34.0 10 32.497281847223 34.0 31.0 34.0 31.0 34.0 11 32.55907078406098 34.0 31.0 34.0 31.0 34.0 12 35.989127388892 37.0 37.0 37.0 35.0 37.0 13 30.947794653365467 31.0 30.0 31.0 30.0 34.0 14 30.36566160399036 31.0 30.0 31.0 26.0 34.0 15 29.367595135347194 31.0 28.0 31.0 25.0 34.0 16 32.579611051953144 35.0 30.0 37.0 26.0 37.0 17 34.10340189430028 35.0 33.0 37.0 30.0 37.0 18 32.63075155523175 35.0 31.0 37.0 25.0 37.0 19 32.66474247604103 35.0 32.0 37.0 26.0 37.0 20 33.17586728689122 35.0 32.0 37.0 28.0 37.0 21 34.80507762147621 37.0 33.0 39.0 28.0 39.0 22 35.41817519475425 37.0 34.0 39.0 30.0 39.0 23 35.892618954211734 37.0 35.0 39.0 31.0 39.0 24 36.369080311606794 37.0 35.0 39.0 32.0 39.0 25 36.50039231071008 37.0 35.0 39.0 32.0 39.0 26 37.74328868463823 39.0 36.0 40.0 33.0 41.0 27 37.836574567057106 39.0 37.0 40.0 33.0 41.0 28 37.985568570307684 39.0 37.0 40.0 34.0 41.0 29 38.06638457658465 39.0 37.0 40.0 34.0 41.0 30 38.17351342263073 40.0 37.0 40.0 34.0 41.0 31 38.3812979880065 40.0 37.0 40.0 35.0 41.0 32 38.34865213248893 40.0 38.0 40.0 35.0 41.0 33 38.46351510396234 40.0 38.0 40.0 35.0 41.0 34 38.54831026172729 40.0 38.0 40.0 35.0 41.0 35 38.48753012385809 40.0 38.0 40.0 35.0 41.0 36 38.54337835565768 40.0 38.0 40.0 35.0 41.0 37 38.41540099759009 40.0 38.0 40.0 35.0 41.0 38 38.451157316594745 40.0 38.0 40.0 35.0 41.0 39 38.45908759737712 40.0 38.0 40.0 35.0 41.0 40 38.46180575015412 40.0 38.0 40.0 35.0 41.0 41 38.45426778008183 40.0 38.0 40.0 35.0 41.0 42 38.495208204898276 40.0 38.0 40.0 35.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7894729935136979 1101 2 0.82957630405798 1101 3 0.4020413363158468 1101 4 0.5683918755507662 1101 5 1.1517969187625923 1101 6 0.5959202975691085 1101 7 0.9644460358319407 1101 8 0.7160734654778906 1101 9 0.8696651614862674 1101 10 0.9148394841305318 1101 11 1.0265502831270439 1101 12 0.7328053477224401 1101 13 0.1737423630074666 1101 14 -0.2533300666552307 1101 15 0.21524089913490485 1101 16 0.4023829900834386 1101 17 -0.0019115199619932355 1101 18 0.17175001947352797 1101 19 -1.0190018970487458 1101 20 -0.3039403999470096 1101 21 -0.6087175374902998 1101 22 -0.611773722606209 1101 23 -0.030413253656753625 1101 24 -0.8968628431702328 1101 25 -0.2649658791611671 1101 26 -0.2997900966250242 1101 27 -0.823792703808877 1101 28 0.13095727129859114 1101 29 0.012576705869498994 1101 30 0.33994355089611616 1101 31 0.7639476006993604 1101 32 0.35354160894456044 1101 33 0.4105905108617165 1101 34 -0.016905521123220524 1101 35 0.07660194144867205 1101 36 0.38026743356101633 1101 37 0.6395757780214879 1101 38 0.5536714985513598 1101 39 0.3100976813669263 1101 40 0.6440238327007677 1101 41 0.498028500439311 1101 42 0.21697246525096858 1102 1 0.7134062031611919 1102 2 0.8731755619429507 1102 3 0.7090914290802246 1102 4 0.7186701686862023 1102 5 0.24224218777928996 1102 6 0.5741206686266196 1102 7 -0.29900479904746646 1102 8 0.5838842261457913 1102 9 0.3501846420057433 1102 10 0.34480237837914274 1102 11 0.49686568201386905 1102 12 0.3728795592252254 1102 13 -0.14861385220589085 1102 14 -0.38737459355689907 1102 15 -0.33949008416750814 1102 16 0.07585237783854382 1102 17 -0.09513972033305151 1102 18 -0.6394744703223907 1102 19 0.07005190629077163 1102 20 -1.0474468934534968 1102 21 -1.361964290737049 1102 22 -1.0510130547026861 1102 23 -0.8917305078311557 1102 24 0.0864395687036037 1102 25 -0.8391773819440971 1102 26 -0.4751147719496984 1102 27 -0.6920672863691735 1102 28 0.0038700727828171466 1102 29 0.2277900639399988 1102 30 -0.17814550290722053 1102 31 0.34418878808340025 1102 32 0.7125397536569906 1102 33 0.7171767817336985 1102 34 0.5670462413999715 1102 35 0.3702011993336427 1102 36 0.1901004576797547 1102 37 1.004603607335028 1102 38 0.8236158399242726 1102 39 1.0930290357268504 1102 40 0.7029292130347216 1102 41 0.7498837880088871 1102 42 1.0499965839893761 1103 1 0.6402448631534909 1103 2 0.339867899255232 1103 3 0.18849073597279187 1103 4 0.1996097143157769 1103 5 -0.018443218458678245 1103 6 0.5671895943101006 1103 7 0.6505523823156452 1103 8 0.6092981653063632 1103 9 0.49573720265264853 1103 10 0.4907399987102963 1103 11 0.5299808148594565 1103 12 0.5715703562987713 1103 13 0.05238730297315897 1103 14 -0.0462117133104627 1103 15 -0.4022548127004306 1103 16 -0.5410132363316578 1103 17 -0.7193214685040203 1103 18 0.6670330383414509 1103 19 0.21598952662192517 1103 20 -1.133700262725739 1103 21 -0.7150640211952748 1103 22 0.23085063416909435 1103 23 -0.0033978162639627385 1103 24 -0.1850275086933948 1103 25 -0.20921973850936837 1103 26 -0.08967002801438895 1103 27 -0.3743930468620462 1103 28 -0.7069501043446351 1103 29 -0.34248332843581863 1103 30 -0.005253704678494842 1103 31 -0.2553491402955004 1103 32 -0.0075218558924845524 1103 33 -0.2339206383664134 1103 34 -0.19229145594311348 1103 35 -0.672540425618223 1103 36 -0.16261421309421564 1103 37 -0.024660928668048143 1103 38 0.31610717608138117 1103 39 -0.09642032892466545 1103 40 -0.5296468362529225 1103 41 0.37753107333811187 1103 42 0.33532581001941253 1104 1 0.3640184480591486 1104 2 0.39684903133071003 1104 3 -0.14886775459324753 1104 4 -0.2188808517219627 1104 5 0.198160555126222 1104 6 0.3022839339327419 1104 7 0.1426278540137602 1104 8 0.20061892002334503 1104 9 0.018756070577175876 1104 10 0.008475847766895583 1104 11 -0.12435880778205188 1104 12 0.38553262044971603 1104 13 0.17465145391655668 1104 14 0.6130335697084064 1104 15 0.09887726277126774 1104 16 0.38238299008343546 1104 17 -0.31554788359836294 1104 18 -0.9782499805264706 1104 19 -0.9662746243214713 1104 20 0.24969596368935498 1104 21 0.8894642806915201 1104 22 -0.2763191771516631 1104 23 -0.15132234456584825 1104 24 0.21950079319339721 1104 25 -0.312238606433894 1104 26 -0.4979719148068469 1104 27 -0.7128836128997804 1104 28 -0.0744972741559522 1104 29 0.1816676149604035 1104 30 -0.07732917637660819 1104 31 -0.14968876293700362 1104 32 -0.208276572873622 1104 33 -0.22486403459283366 1104 34 -0.6541782483959437 1104 35 -0.6061253312785979 1104 36 -0.3833689300753491 1104 37 -0.1631514947057866 1104 38 -0.1608739559940915 1104 39 -0.15717504590580234 1104 40 0.14129655997349744 1104 41 -0.25378968137886915 1104 42 -0.1993911711126657 1105 1 0.24401844805915118 1105 2 0.23684903133070634 1105 3 0.19113224540675589 1105 4 0.06111914827803844 1105 5 -0.34183944487377715 1105 6 0.36228393393274416 1105 7 0.4826278540137636 1105 8 0.38061892002334474 1105 9 -0.201243929422823 1105 10 0.30847584776689985 1105 11 0.09564119221794698 1105 12 0.2855326204497146 1105 13 0.014651453916556534 1105 14 -0.5269664302915942 1105 15 -0.7411227372287321 1105 16 -1.7176170099165624 1105 17 -1.1555478835983592 1105 18 -0.2982499805264709 1105 19 -0.5662746243214727 1105 20 -0.3303040363106433 1105 21 -0.4905357193084825 1105 22 -0.07631917715166026 1105 23 0.4886776554341523 1105 24 -1.0804992068066 1105 25 -0.9722386064338906 1105 26 -0.19797191480684262 1105 27 -0.6528836128997852 1105 28 -0.6744972741559536 1105 29 -0.27833238503959734 1105 30 -0.2573291763766079 1105 31 0.4703112370629938 1105 32 0.6717234271263806 1105 33 0.27513596540716634 1105 34 0.5658217516040551 1105 35 0.7538746687214015 1105 36 0.4566310699246472 1105 37 0.5568485052942123 1105 38 0.9191260440059068 1105 39 0.6028249540942028 1105 40 0.4612965599734977 1105 41 0.6462103186211294 1105 42 0.5406088288873363 1106 1 -0.47296268401632346 1106 2 -0.5657924781032548 1106 3 0.05641526427467625 1106 4 -0.12114500266535799 1106 5 0.43438697022056516 1106 6 -0.564885877388015 1106 7 0.1411184200514981 1106 8 -0.29636221205213076 1106 9 -0.2212439294228261 1106 10 -0.4526562277048001 1106 11 -0.18700031721601107 1106 12 -0.37182587011632506 1106 13 -0.09855609325325432 1106 14 -0.15941926048027355 1106 15 0.2581225457901368 1106 16 -0.2579943684071253 1106 17 0.37501815413748574 1106 18 0.13873115154900262 1106 19 -0.1802368884724146 1106 20 0.47007332217992115 1106 21 0.03965295993680229 1106 22 0.34405818133890165 1106 23 0.6192436931699987 1106 24 -0.22276335774999723 1106 25 -0.20921973850936837 1106 26 0.13674506632523276 1106 27 0.3048522361568189 1106 28 -0.5560067081182183 1106 29 0.16695063382832842 1106 30 0.22116138966112686 1106 31 -0.5383680082200257 1106 32 -0.290540723817017 1106 33 -0.5924112044041507 1106 34 -0.28663107858462666 1106 35 -1.0310309916559603 1106 36 -0.3135576093206325 1106 37 0.031942844916855506 1106 38 -1.2310626352393754 1106 39 -0.549250517603916 1106 40 -0.737194006064243 1106 41 -0.773412322888305 1106 42 -0.872221359791908 1107 1 0.036471278247830696 1107 2 -0.018622666782498243 1107 3 0.113019037859587 1107 4 -0.12114500266535799 1107 5 -0.13165076562849265 1107 6 0.26530280185726696 1107 7 -0.047560825231521164 1107 8 -0.10768296676911149 1107 9 -0.16464015583792246 1107 10 -0.16963735978026762 1107 11 -0.28133993985752426 1107 12 0.32628733743084837 1107 13 -0.6079900555174049 1107 14 -0.3480985057632928 1107 15 -0.6475378315683535 1107 16 -0.18252267029392044 1107 17 0.07313136168465917 1107 18 -0.06881601826231787 1107 19 -0.12363311488751094 1107 20 -1.0959644136691367 1107 21 -0.45091307779904355 1107 22 -0.9012248375290213 1107 23 -0.5128317785281098 1107 24 -0.6944614709575418 1107 25 -0.5677103045470986 1107 26 -1.1085379525426902 1107 27 -0.4687326695035594 1107 28 -0.5937425571748207 1107 29 -0.6255021963603511 1107 30 -0.061857478263405596 1107 31 0.10314142574223695 1107 32 -1.0829935540056965 1107 33 -0.8188262987437724 1107 34 -0.19229145594311348 1107 35 -0.4461253312786013 1107 36 -0.7475198734715747 1107 37 -0.11900055130956133 1107 38 -0.6650248993903176 1107 39 -0.643590140245422 1107 40 -0.0013449494604671486 1107 41 0.056776356356976976 1107 42 -0.40052324658436333 1108 1 0.5958702999110059 1108 2 0.35314532762700424 1108 3 0.3852063194808295 1108 4 0.5263043334632229 1108 5 0.0011235180891873142 1108 6 0.29709874874755826 1108 7 -0.032186960801055875 1108 8 0.23543373483816055 1108 9 0.2787560705771739 1108 10 0.3988462181372725 1108 11 0.3615671181438742 1108 12 0.41294002785711825 1108 13 -0.30016336089825657 1108 14 -0.03363309695826189 1108 15 -0.6803819964879914 1108 16 -0.37539478769434 1108 17 0.2548224867720137 1108 18 -0.6523240546005447 1108 19 -0.7277561058029534 1108 20 -0.19030403631064274 1108 21 -0.25349868227144157 1108 22 -0.5200228808553646 1108 23 -0.037989011232511416 1108 24 -0.8530917993991949 1108 25 -0.1959423101375961 1108 26 -0.17352747036240146 1108 27 -0.36251324252940975 1108 28 -0.4885713482300318 1108 29 -0.9301842368914492 1108 30 -0.6928847319321676 1108 31 -0.608948022196266 1108 32 -0.4393876839847337 1108 33 0.00402485429605548 1108 34 0.43174767752997667 1108 35 0.03535615020287963 1108 36 0.10922366251724469 1108 37 0.042774431220138354 1108 38 -0.4008739559940935 1108 39 0.3976397689090163 1108 40 0.2879632266401586 1108 41 0.08472883713965018 1108 42 0.4880162362947402 1109 1 0.5240184480591523 1109 2 0.3568490313307038 1109 3 0.3911322454067516 1109 4 0.5011191482780362 1109 5 0.21816055512622512 1109 6 0.16228393393274132 1109 7 0.3826278540137622 1109 8 0.5006189200233422 1109 9 0.09875607057717417 1109 10 0.5684758477668979 1109 11 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5 0.40482722179289254 1110 6 0.13228393393274018 1110 7 0.2992945206804265 1110 8 -0.3960477466433261 1110 9 -0.02124392942282327 1110 10 -0.304857485566437 1110 11 0.11897452555128041 1110 12 -0.34446737955028794 1110 13 0.24798478724989081 1110 14 -0.7669664302915926 1110 15 -0.6544560705620661 1110 16 -0.9809503432498943 1110 17 -0.6488812169316915 1110 18 0.7217500194735251 1110 19 -0.37960795765480526 1110 20 -0.27363736964397845 1110 21 0.39279761402485036 1110 22 0.6170141561816678 1110 23 1.0453443221008172 1110 24 -0.07716587347326964 1110 25 0.057761393566103436 1110 26 0.3820280851931557 1110 27 0.2171163871002193 1110 28 0.48550272584404297 1110 29 0.8716676149604083 1110 30 0.11267082362338954 1110 31 -0.24968876293700504 1110 32 -0.6949432395402866 1110 33 -0.1848640345928274 1110 34 0.02248841827071857 1110 35 0.5538746687213987 1110 36 0.43329773659131376 1110 37 -0.2498181613724526 1110 38 -0.0842072893274235 1110 39 -0.050508379239133205 1110 40 -0.1453701066931714 1110 41 -0.1337896813788717 1110 42 0.03060882888733829 1111 1 -1.2797910757503743 1111 2 -1.3082303337486607 1111 3 -0.5856931514186456 1111 4 -1.0001506929918023 1111 5 -0.5015219845563124 1111 6 -0.8589859073371002 1111 7 -0.8364197650338596 1111 8 -0.2619207625163398 1111 9 -0.7291804373593358 1111 10 -0.4715241522331013 1111 11 -0.6595969030201445 1111 12 -0.86748325256616 1111 13 -0.14407870481360163 1111 14 -0.716172779497942 1111 15 -0.4846148007207951 1111 16 -1.200791613091166 1111 17 -0.5758653439158223 1111 18 -0.7845991868756776 1111 19 -1.3177031957500454 1111 20 -0.9204627664693703 1111 21 -0.9968849256576888 1111 22 -0.9988588596913459 1111 23 -0.712592185835689 1111 24 0.16807222176483094 1111 25 -0.3017624159577039 1111 26 -0.17352747036240146 1111 27 -0.3678042478204162 1111 28 -1.0732274328861138 1111 29 -0.42753873424594246 1111 30 -0.23256727161470536 1111 31 0.03126361801537314 1111 32 -0.2489114935085368 1111 33 -0.035657685386482285 1111 34 -0.25608301030070635 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0.33315821896292874 2212 26 0.2867899899550608 2212 27 0.394100514084343 2212 28 0.5458201861615066 2212 29 0.12801682130961467 2212 30 0.6721946331471997 2212 31 0.4915810783328354 2212 32 0.2272789826819377 2212 33 0.15481850508970751 2212 34 -0.20846396268166245 2212 35 -0.2239031090563799 2212 36 -0.18178162848804647 2212 37 -0.11331022486451303 2212 38 0.3769038217836851 2212 39 0.20187257314182006 2212 40 0.4308203694973045 2212 41 -0.1322023797915648 2212 42 0.24727549555399975 2213 1 0.3392565432972461 2213 2 0.08859506307673826 2213 3 -0.0777566434821324 2213 4 0.01572232288121711 2213 5 0.466731983697656 2213 6 0.2203791720279824 2213 7 0.41754848893439345 2213 8 0.3729998724042929 2213 9 0.28669257851368 2213 10 0.195142514433563 2213 11 0.08643484301160242 2213 12 0.2753738902909859 2213 13 0.5067149459800468 2213 14 0.3631922998671371 2213 15 0.4995121834061891 2213 16 0.8627004504009008 2213 17 0.8844521164016399 2213 18 1.07254367026718 2213 19 1.3807095026626541 2213 20 0.746203900197294 2213 21 1.6539087251359632 2213 22 0.6043157434832551 2213 23 0.3667728935293937 2213 24 0.8506119043045146 2213 25 1.0791899649946757 2213 26 0.09631379947887098 2213 27 0.9655290855129124 2213 28 0.6886773290186454 2213 29 0.38198507527786774 2213 30 0.37060733155989567 2213 31 0.5074540942058476 2213 32 0.43362818903113975 2213 33 0.28180263207383405 2213 34 0.22010746588976815 2213 35 0.569747684594418 2213 36 0.34202789532147193 2213 37 0.7914516798973921 2213 38 0.4562689011487606 2213 39 0.5352059064751487 2213 40 0.41494735362428514 2213 41 0.7408134932243087 2213 42 0.34251359079209465 2214 1 -0.8226482186075188 2214 2 -0.7598176353359634 2214 3 -0.4722010879265781 2214 4 -0.3922141850552947 2214 5 -0.5118394448737718 2214 6 -0.38438273273392554 2214 7 0.4492945206804251 2214 8 -0.12938107997665327 2214 9 -0.887910596089494 2214 10 -0.771524152233102 2214 11 -1.131025474448716 2214 12 -0.4611340462169551 2214 13 0.1979847872498901 2214 14 0.8997002363750717 2214 15 1.0122105961046017 2214 16 0.20238299008343574 2214 17 0.3177854497349699 2214 18 1.5884166861401923 2214 19 1.537058709011859 2214 20 0.29302929702269154 2214 21 -0.0738690526418111 2214 22 0.08368082284833633 2214 23 -0.08798901123251568 2214 24 0.6561674598600646 2214 25 0.14109472689943914 2214 26 0.4986947518598228 2214 27 0.16711638710021504 2214 28 0.035502725844047234 2214 29 0.47166761496040266 2214 30 -0.07066250970994048 2214 31 0.08364457039632356 2214 32 -0.028276572873622285 2214 33 0.2484692987405026 2214 34 -0.22751158172928143 2214 35 0.6038746687213958 2214 36 0.2999644032579809 2214 37 0.3168485052942174 2214 38 0.23245937733923938 2214 39 -0.5671750459057989 2214 40 -0.34537010669317425 2214 41 -0.6004563480455332 2214 42 -0.9860578377793345 2215 1 -0.11191375533068282 2215 2 -0.5872187652794594 2215 3 -0.15496944950849922 2215 4 -1.1419316991795867 2215 5 0.29239784326181706 2215 6 -0.5092414897960751 2215 7 -0.21737214598623922 2215 8 -0.6980251477732651 2215 9 -0.8483625734906219 2215 10 -0.7596597454534404 2215 11 -0.7677486382905236 2215 12 -0.45209449819435577 2215 13 0.014651453916556534 2215 14 0.7855759425897624 2215 15 0.5449789576865207 2215 16 0.5718745155071687 2215 17 0.7827572011474047 2215 18 0.4386991720159017 2215 19 0.7791491044920846 2215 20 0.14867901453681753 2215 21 -0.12951877015593993 2215 22 -0.6553022279991225 2215 23 0.7727454520443189 2215 24 0.4852635050578087 2215 25 0.7111512240745839 2215 26 0.14473994959993775 2215 27 -0.2583073417133406 2215 28 0.6109264546576014 2215 29 0.3453964285197273 2215 30 -0.10343087129186301 2215 31 0.05709089807994161 2215 32 -0.12488674236514186 2215 33 0.19140715184784796 2215 34 0.04819463295998361 2215 35 0.07929839753495571 2215 36 -0.042690963973655016 2215 37 -0.09501590148544636 2215 38 0.12454977281946356 2215 39 -0.1629377577702087 2215 40 -0.2600593722298967 2215 41 -0.247349003412765 2215 42 -0.3007471033160556 2216 1 -0.022648218607514536 2216 2 -0.09870652422485193 2216 3 -0.18886775459324667 2216 4 -0.2922141850552933 2216 5 0.8381605551262226 2216 6 -0.06216051051170268 2216 7 0.22707229845820365 2216 8 0.13173003113445247 2216 9 0.3120894039105053 2216 10 0.2173647366557887 2216 11 -0.2310254744487139 2216 12 0.09442150933860205 2216 13 0.21465145391655582 2216 14 0.3885891252639624 2216 15 0.1344328183268253 2216 16 0.6690496567501043 2216 17 0.7066743386238628 2216 18 1.3995277972513023 2216 19 1.2981698201229719 2216 20 0.9874737414671344 2216 21 0.9205753918026289 2216 22 -0.6718747327072165 2216 23 -0.7157667890102886 2216 24 -0.616054762362154 2216 25 0.1077613935661077 2216 26 0.5598058629709328 2216 27 0.33378305376688644 2216 28 0.3521693925107101 2216 29 0.43277872607151835 2216 30 0.5071152680678352 2216 31 0.20586679261855068 2216 32 -0.26160990620694946 2216 33 0.23735818762938976 2216 34 0.2835995293818314 2216 35 -0.35723644238971275 2216 36 -0.705591152297572 2216 37 -0.6275959391502326 2216 38 -0.6453184004385406 2216 39 -0.6171750459058032 2216 40 -1.0009256622487257 2216 41 0.12176587417668827 2216 42 -0.019391171112665972 >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 4.0 27 30.0 28 88.0 29 171.0 30 339.0 31 549.0 32 961.0 33 1856.0 34 3820.0 35 7140.0 36 9907.0 37 6842.0 38 3979.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.7510158329830461 0.5212274064733081 98.18130867311196 0.546448087431694 2 89.51689738272712 1.0928655495152162 9.194081712716471 0.1961553550411926 3 0.7313997477931904 1.1405352388958947 0.3110550651534258 97.81700994815749 4 9.132432886846383 1.4991873563862579 88.76870481421287 0.5996749425545032 5 1.263800930336827 96.09931065403799 0.9555568009863812 1.6813316146387938 6 0.5184105811802948 0.3054419099927142 0.37829961329372863 98.79784789553327 7 0.3447019589160104 97.56746910293417 0.9836616876383711 1.104167250511448 8 96.8587120999832 1.8270470212408227 0.5520372134730707 0.7622036653029198 9 31.654756900658544 18.584839568446128 24.892812105926858 24.867591424968474 10 29.906123020877118 17.766568586240716 28.90570267619448 23.421605716687687 11 29.794866046407353 17.73624033180137 23.531554758435153 28.93733886335613 12 29.04382916713373 20.126667413967045 25.829503418899225 25.0 13 59.22022591585615 23.19982061271968 7.368893124421897 10.21106034700227 14 24.952359600941598 25.52124201322722 36.85965698912678 12.666741396704406 15 14.474274184508463 20.306019504539847 52.096177558569664 13.123528752382018 16 14.860169254049207 19.74163537521717 52.03441125371294 13.36378411702068 17 14.67522277643894 24.354088438042933 12.172840889984867 48.797847895533266 18 44.647512263489844 23.859845830413455 13.74071478626489 17.751927119831816 19 25.142921197175205 29.248402645443335 24.758995628292794 20.84968052908867 20 22.85209886229894 24.558650451157316 27.23757215714846 25.351678529395283 21 24.48018830914084 24.107493134562574 27.854060415849354 23.558258140447233 22 27.344056492742254 26.00459563974668 25.301238580956113 21.350109286554954 23 24.550931764046517 28.006165055345384 27.089813647190695 20.3530895334174 24 22.42896373928151 26.248388723869304 25.421734013338565 25.90091352351062 25 21.490220254441518 27.86246707392255 26.937734685871213 23.709577985764728 26 25.233985316370568 28.4397242616152 24.96777447738609 21.358515944628145 27 23.409740514487474 29.454127669113937 26.97416353752172 20.16196827887687 28 22.207588410020737 29.089839152608864 25.92052905901474 22.782043378355656 29 24.8612901417923 25.25920529059015 28.37807543574511 21.50142913187244 30 24.43815501877487 28.053017990248275 28.019391357955502 19.489435633021355 31 22.57748136524127 29.39528106260158 24.894916774085075 23.132320798072072 32 22.72599899120103 27.349660931457713 28.554615255282183 21.369724822059073 33 24.23079078630275 27.231967718432998 28.184722300061647 20.3525191952026 34 24.72747653075522 28.39288216337397 26.890850497407875 19.988790808462937 35 22.378523790842348 27.559827383287566 28.069831306394665 21.991817519475422 36 25.506515342580915 26.0697772173182 27.381252627154268 21.042454812946616 37 22.72599899120103 28.781595023258422 28.829232752339852 19.663173233200695 38 21.03065627977358 26.66031496945581 30.745950793028076 21.563077957742532 39 22.63976460697772 25.357993554714863 29.43533697632058 22.566904861986828 40 23.895194059128485 28.95053944234272 27.00294241277848 20.151324085750318 41 23.790842347138934 28.14549122905341 27.854060415849354 20.209606007958303 42 22.627921313680435 28.20994227428123 27.671916157596815 21.490220254441518 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.5 9 5.0 10 5.0 11 12.0 12 19.0 13 30.0 14 41.0 15 57.0 16 73.0 17 73.0 18 95.0 19 117.0 20 171.0 21 225.0 22 225.0 23 321.5 24 418.0 25 515.0 26 612.0 27 750.5 28 889.0 29 889.0 30 974.0 31 1059.0 32 1121.0 33 1183.0 34 1183.0 35 1322.0 36 1461.0 37 1579.5 38 1698.0 39 1960.0 40 2222.0 41 2222.0 42 2383.5 43 2545.0 44 2799.5 45 3054.0 46 2983.0 47 2912.0 48 2912.0 49 3085.0 50 3258.0 51 3159.5 52 3061.0 53 3061.0 54 3167.0 55 3273.0 56 2783.0 57 2293.0 58 2017.5 59 1742.0 60 1742.0 61 1460.5 62 1179.0 63 960.5 64 742.0 65 608.5 66 475.0 67 475.0 68 384.0 69 293.0 70 278.0 71 263.0 72 263.0 73 226.0 74 189.0 75 148.5 76 108.0 77 116.5 78 125.0 79 125.0 80 81.0 81 37.0 82 72.0 83 107.0 84 107.0 85 56.0 86 5.0 87 4.0 88 3.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002802219357731323 2 0.0 3 0.002802219357731323 4 0.0 5 0.0 6 0.0 7 0.00840665807319397 8 0.0 9 0.002802219357731323 10 0.002802219357731323 11 0.005604438715462646 12 0.005604438715462646 13 0.02521997421958191 14 0.005604438715462646 15 0.005604438715462646 16 0.0 17 0.0 18 0.030824412935044555 19 0.005604438715462646 20 0.0 21 0.0 22 0.0 23 0.002802219357731323 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.002802219357731323 35 0.0 36 0.002802219357731323 37 0.0 38 0.0 39 0.002802219357731323 40 0.002802219357731323 41 0.0 42 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 35686.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.41669001849465 #Duplication Level Percentage of deduplicated Percentage of total 1 76.36023462783172 42.316314521100715 2 11.898260517799352 13.187244297483607 3 4.020024271844661 6.683293168189206 4 2.2148058252427183 4.909488314745278 5 1.5018203883495147 4.161295746231015 6 0.7787216828478964 2.5892506865437426 7 0.7382686084142395 2.8638681836014124 8 0.45509708737864074 2.0175979375665527 9 0.3489077669902913 1.7401782211511518 >10 1.59789644012945 14.605167292495658 >50 0.06067961165048543 2.4127108670066693 >100 0.025283171521035597 2.513590763884997 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGCTACAGCTGTAGTTCGAGGAGGAGGAGGGTGAAGATGAGG 433 1.213360981897663 No Hit TGCTACAGGTGGGTTTCACTAGATCGTGTTATGGACGATGAC 124 0.34747520035868407 No Hit TGCTACAGCATAGTTGCAGTGAGCCGAGATCATGCCACTGCA 123 0.3446729810009528 No Hit TGCTACAGGCGATTCGAAGCGGGATCTCGCCCACCATTTGGG 114 0.31945300678137084 No Hit TGCTACAGCCCTGTTTGGTTTGCATGACTCTTTCCTCAATGA 103 0.2886285938463263 No Hit TGCTACAGCTGAGTTTACTTTGAAAAAATTAGAGTGTTCAAA 92 0.2578041809112817 No Hit TGCAACCGAGCGGCGGCGCGCCGGTGGGCGCGGACCGTCGGG 89 0.24939752283808778 No Hit TGCTTCAACTCCAGAAGCAGGCTCCATGCACCAGGAGCCCGA 87 0.2437930841226251 No Hit TGCTACAGTGCTGGTTCGATCAGGCTACTACAAAGTTCTGGG 81 0.2269797679762372 No Hit TTCGACAGTGGTGTTTTGGGCAAAATTGAGAGAGCCATCGGC 78 0.2185731099030432 No Hit TGCTACAGTGGCGATTAGTTTAATTAAACTAACGAATAGTTT 73 0.2045620131143866 No Hit TGCTACAGCAAAGCTTCGGAGGTCGTGGGTGACAAGTTGGTG 68 0.19055091632572996 No Hit TGCTACAGGGGAGTTGCAGAAAAGTCATTGCATGGAAAGATG 66 0.18494647761026733 No Hit TGCTACAGCATAGTTTACTTTGAAAAAATTAGAGTGTTCAAA 65 0.18214425825253602 No Hit TGCTACAGATCGGTTTCAGTCTCGACGGGCTAGCACACATCT 55 0.15412206467522277 No Hit TGCTACAGGGGCGATTCCCCATGGCTAAGATCTCACACTGCT 54 0.15131984531749146 No Hit TGCTACAGGGGTGGAGCGGACTTGTAAGGACCGCCGCTTTCG 53 0.14851762595976015 No Hit TGCTACAGTGTAGCGACGATCGGACTGTAGAACTCTGAACCC 50 0.14011096788656616 Illumina DpnII expression Adapter 1 (95% over 23bp) TGCTACAGTGCTGGGGCGCATTGCGATCCAACCATCGGCAGC 50 0.14011096788656616 No Hit TGCTACAGGTGCGTTTACTTTGAAAAAATTAGAGTGTTCAAA 49 0.13730874852883485 No Hit TGCTACAGTTTGGTTTACTTTGAAAAAATTAGAGTGTTCAAA 48 0.13450652917110353 No Hit TGGTCCAGGGCGGCATCTGGATACCGGCGTACCTGGAGCACG 47 0.1317043098133722 No Hit TGCTACAGCTGGAATTCTTGGACCGGCGCAAGACGGACCAGA 45 0.12609987109790954 No Hit TGCAGCAGTGAGGGGCAGGTGCGCAACTTCTCCATTCCGGGC 44 0.12329765174017822 No Hit TGCTACAGAGCTGTGAATTAGATAGGGAAAAGATCGGGATGC 42 0.11769321302471558 No Hit TGCTACAGTAGGGTTTGGCAAATCTGTCCACCAGAATTGCTC 42 0.11769321302471558 No Hit TGCTACAGATCGAATTTGACATGAAAATCAAAGGCTAGGGCT 42 0.11769321302471558 No Hit GGCGACAGGCATGGCCGATGGCCGCACGCTGTGGCTGCTGTC 41 0.11489099366698424 No Hit TGCTACAGCAGTGTTTACTTTGAAAAAATTAGAGTGTTCAAA 40 0.11208877430925292 No Hit TGCTACAGATGCGTTTCCCCTGTTCTTACCCTATATAAGTTG 38 0.10648433559379028 No Hit TGCTACAGTGAGGTTTACTTTGAAAAAATTAGAGTGTTCAAA 38 0.10648433559379028 No Hit TGCTACAGCCCACGACGATCTACTTGCTACAGTTTTTTTTTT 37 0.10368211623605897 No Hit TGCTACAGTGACAGTTCTACAGTCCGACGATCGTCGGACGTA 36 0.10087989687832764 No Hit TGCTACAGCATAAATTAGCTGCCACCAGGCCCAGCTAATTTT 36 0.10087989687832764 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.025219974219581907 0.0 0.0 15 0.0 0.0 0.028022193577313233 0.0 0.0 16 0.0 0.0 0.028022193577313233 0.0 0.0 17 0.0 0.0 0.033626632292775877 0.0 0.0 18 0.0 0.0 0.03923107100823853 0.0 0.0 19 0.0 0.0 0.03923107100823853 0.0 0.0 20 0.0 0.0 0.09807767752059632 0.0 0.0 21 0.0 0.0 0.2297819873339685 0.0 0.0 22 0.0 0.0 0.459563974667937 0.0 0.0 23 0.0 0.0 0.5296194586112201 0.0 0.0 24 0.0 0.0 0.538026116684414 0.0 0.0 25 0.0 0.0 0.5968727231967719 0.0 0.0 26 0.0 0.0 0.624894916774085 0.0 0.0 27 0.0 0.0 0.6361037942050104 0.0 0.0 28 0.0 0.0 0.728577033010144 0.0 0.0 29 0.0 0.0 0.7734125427338452 0.0 0.0 30 0.0 0.0 0.8210502718152777 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACAGC 815 0.0 36.0 3 CGACAGA 95 0.0 36.0 3 CAGCTAT 25 1.4214279E-4 36.0 6 ACAGCCA 30 9.760084E-6 36.0 5 ACAGTCT 60 1.8189894E-12 36.0 5 GATGGCC 25 1.4214279E-4 36.0 17 ACAGCAA 25 1.4214279E-4 36.0 5 ACAGTAT 25 1.4214279E-4 36.0 5 ACGCTGT 25 1.4214279E-4 36.0 26 CAGCATT 25 1.4214279E-4 36.0 6 CAGCATA 25 1.4214279E-4 36.0 6 CAGTCCA 30 9.760084E-6 36.0 6 ATCTCGC 25 1.4214279E-4 36.0 24 ACAGATG 45 3.2305252E-9 36.0 5 ACAGATC 50 2.237357E-10 36.0 5 ACAGAGT 55 1.6370905E-11 36.0 5 ACAGAGC 70 0.0 36.0 5 TACAGGT 245 0.0 36.0 4 TACAGGC 180 0.0 36.0 4 TTACTTT 85 0.0 36.0 15 >>END_MODULE