FastQCFastQC Report
Thu 26 May 2016
SRR2099226_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099226_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539494
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACGTTTCTAATGACGCTGAACGGCTTGCGGCGCACCTT148142.7459063492828464No Hit
GACTAGGTAGTCTCTACAGTCCGACGATCAGTCTAGAAAGGT70861.3134529763074287No Hit
GACTACGTGCCGCAGTCCGACGATCTTAGGACTACGTTTTTT54631.0126155249177933No Hit
GACTAGGGGTACGAACCCCTATAGTGAGTCGTATTACCGGCC16570.3071396530823327No Hit
GACCACGGGGCTGCGGAAGATGCTGGGCCCGTCGGCCATGAC12550.23262538600985366No Hit
GACTAGGGGTACCGGCCGACGATCGTACAACTAGCAGTTGAC11740.2176113172713691No Hit
GATTCCGTCATGAGGACATCAGCCTGCCAGCCGTTGCGCGCC9160.16978872795619598No Hit
GACTACGTGTAAGCTCCGGCTGGCATCTGGCGGATGCCCTTC8980.16645226823653275No Hit
GACTACGTGCTGACCGGCGAGACCCGTCGGGCCTTCAGCCTG8610.15959398992389165No Hit
GACTACGTTTCTAACGGCTTGCGGCGCACCTTCTCGGTGAGC7390.13698020737950747No Hit
GACTACGTATCTGTGCCTCGGGAAACCACTTCTCAGGCCATG7150.1325315944199565No Hit
GACTAGGTAGTCCAGTCCGACGATCAGTCTAGAAAGGTAGCT6710.12437580399411301No Hit
GACTAGGGGTACGACTCCCTAGTCGTACGATCGTCGGACTGT6620.12270757413428138No Hit
GAGCACGTGGATGGAGAAGAGAGCTATGGTTTAGGACAGCTC6250.11584929582164029No Hit
GACTAGGGGTACGAGTCGTATTACCGGCCGACGATCGTACAA6160.11418106596180866No Hit
GACTAGGGGTACCCGGCCGACGATCGTACAACTAGCAGTTGA6160.11418106596180866No Hit
GACTACGTTTGGATCAGGGGACTACGTTTTTTTTTTTTTTTT5850.1084349408890553No Hit
GACTAGGGGTACGTAGAACTCTGAACCCCTATAGTGAGTCGT5580.10343025130956043No Hit
GACTACGTTCTAGACATGAGGAGAGCCTGGTCCAAGAGACTG5450.10102058595647032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTCG601.8189894E-1236.030557
GGTACGA3900.036.030558
GTATTCG309.961817E-636.030557
ACGTTTC30700.036.030555
GTTTCGA309.961817E-636.030557
GCGCGTA750.036.030559
GTCCTTA251.4411603E-436.030557
CGTGATA1250.036.030556
CGTTAGG1750.036.030556
CGTTAAG1500.036.030556
CCGTAGT404.83451E-836.030555
TGCGCGT850.036.030558
CGCTCGA404.83451E-836.030556
CATACGG200.002097367336.030556
ACGCTCG404.83451E-836.030555
CGTACCG1300.036.030556
ACGTCGC3750.036.030555
CGTGTCG601.8189894E-1236.030556
CGTGTAG2500.036.030556
CGTAAGC1650.036.030556
Produced by FastQC (version 0.11.5)