FastQCFastQC Report
Thu 26 May 2016
SRR2099225_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099225_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26793
Sequences flagged as poor quality0
Sequence length42
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACGTTTCTAATGACGCTGAACGGCTTGCGGCGCACCTT3591.3399022132646587No Hit
GACTAGGTAGTCTCTACAGTCCGACGATCAGTCTAGAAAGGT1460.5449184488485799No Hit
GACTACGTTACTGTAACTTTTTTGTGTATGTACTTAGCTGTA1000.37323181427984925No Hit
GACTACGTCTTCGCTTCAGCTCCAGCCCAGGCTCCCAAAGGT930.34710558728025975No Hit
GACTACGTTATTGTGAAGTAAAATGGTGAAGCTTATGAATTT850.31724704213787186No Hit
GACTACGTCACCGCGACGATCATCGGACTGTAGAACTCTGAA770.28738849699548386Illumina DpnII expression Sequencing Primer (96% over 25bp)
GACTACGTAAGGGATTTGAGGAAGCCTGCTCCTAGTACGAGA750.2799238607098869No Hit
GACTACGTGGATCTGTAGGTGGTCATTCATTCACATGATGAA680.2537976337102975No Hit
GACTACGTCCTTGGAAGGAATTCCTCCTGATCAGCAGAGACT660.2463329974247005No Hit
GACTACGTTGGGGGTTCGACTGTAGAACTCTGAACCCCTATA500.18661590713992462No Hit
GACTACGTGCCGCAGTCCGACGATCTTAGGACTACGTTTTTT490.18288358899712612No Hit
GACTACGTCACCGATCCGGGGACTGTAGAACTCTGAACCCCT460.17168663456873062Illumina DpnII expression Adapter 1 (95% over 21bp)
GACTACGTAGTGATTTTAGGCTACAATTGGGAAGACAGTAAT390.1455604075691412No Hit
GACTACGTTTTCAGTCCGACGATCGGTGGACTGTAGAACTCT390.1455604075691412No Hit
GACTACGTTGCTGTTTTGCCGGCGCACAAGCACGATCGGCTC380.1418280894263427No Hit
GACTACGTCCTGGTTTCAGTTCTGGTTTCTTCCCTCCTGACC380.1418280894263427No Hit
GACTACGTGCGTGATTAAGGGGGATACAAATTAGAAAGAACT370.13809577128354422No Hit
GACTACGTGTCCGAGGAGCGGACTTGTAAGGACCGCCGCTTT360.13436345314074571No Hit
GACTACGTTGCGGATTGGGCGGATTGAGGATGTCACCCCCAT360.13436345314074571No Hit
GACTACGTTTAGGATATGATGAACGTCTCTGAACCTCAAAAA360.13436345314074571No Hit
GACTAGGGGTACGAACCCCTATAGTGAGTCGTATTACCGGCC350.1306311349979472No Hit
GACTACGTTGCTGGATAGATAGCCACCTATATAGTATAGCTT340.12689881685514875No Hit
GACTACGTGTGTGTTTCAGTCTCGACGGGCTAGCACACATCT330.12316649871235025No Hit
GACTACGTGAGGATCTCGGGACTACGTTTTTTTTTTTTTTTT330.12316649871235025No Hit
GACTACGTTTTGAGTCCGACGATCCCCAGACTGTAGAACTCT330.12316649871235025No Hit
GACTACGTGCTGACCGGCGAGACCCGTCGGGCCTTCAGCCTG320.11943418056955175No Hit
GACTACGTTGGCGATCCGTCGACTGTAGAACTCTGAACCCCT320.11943418056955175No Hit
GACTACGTATGAGCTTCGACAGAATTGAGTACATGCCTTTCT310.11570186242675325No Hit
GACCACGGGGCTGCGGAAGATGCTGGGCCCGTCGGCCATGAC310.11570186242675325No Hit
GACTACGTATAGATTTCAAAGGGAACTGAGGCCCAATGAAGA300.11196954428395475No Hit
GACTACGTTCGCGTGCAGAGGGAGGAGAATGAGGTTCTGTGT300.11196954428395475No Hit
GACTACGTATTAGTTTCACTAGATCGTGTTATGGACGATGAC300.11196954428395475No Hit
GACTACGTCCTTGGGAAGGAATTCCTCCTGATCAGCAGAGAC290.10823722614115629No Hit
GACTACGTTGTGGTTTGTCCCAGTAGCAGGGACACCAAGGCC270.10077258985555929No Hit
GACTACGTAGGTGTTCCAAGGGCCGGAAGTTTGAGCGCGCCA270.10077258985555929No Hit
GACTACGTCAATGTTTGGGTCTTCCGAGGCCAAGAGCTTGCC270.10077258985555929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGA309.6687745E-636.00000413
ACGTTGA309.6687745E-636.0000045
CGTAGCT309.6687745E-636.0000046
TACGTCT1200.036.0000044
CTACGTG8450.036.0000043
CTACGTC4800.036.0000043
CGTGTGT309.6687745E-636.0000046
CGTGCCG309.6687745E-636.0000046
AATACGA309.6687745E-636.00000413
CGTGATG309.6687745E-636.0000046
CGTGAGC309.6687745E-636.0000046
ACGTGTG601.8189894E-1236.0000045
ACGTGGC601.8189894E-1236.0000045
ACTACAT309.6687745E-636.0000042
AGGGGTA551.4551915E-1136.05
CGCGACG200.002070608536.012
CGTTTAG200.002070608536.06
CGCTGAA453.1668606E-936.018
ACGCTGA453.1668606E-936.017
CCGGAAG200.002070608536.023
Produced by FastQC (version 0.11.5)