##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099225_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26793 Sequences flagged as poor quality 0 Sequence length 42 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.79662598439891 37.0 37.0 37.0 35.0 37.0 2 34.87224274997201 35.0 35.0 37.0 32.0 37.0 3 35.71820998021872 37.0 35.0 37.0 35.0 37.0 4 35.73731944910984 37.0 35.0 37.0 35.0 37.0 5 36.313813309446495 37.0 35.0 39.0 33.0 39.0 6 37.508453700593435 39.0 37.0 39.0 35.0 39.0 7 37.57679244578808 39.0 38.0 39.0 35.0 39.0 8 37.0690852088232 39.0 37.0 39.0 34.0 39.0 9 32.080655395065875 33.0 31.0 34.0 30.0 34.0 10 32.23017205986638 34.0 31.0 34.0 30.0 34.0 11 32.23409099391632 34.0 31.0 34.0 30.0 34.0 12 35.676743925652225 37.0 35.0 37.0 35.0 37.0 13 30.675512260665098 31.0 30.0 31.0 28.0 34.0 14 30.091367148135706 31.0 30.0 31.0 26.0 34.0 15 28.739596163176948 30.0 27.0 31.0 23.0 34.0 16 31.833426641286902 33.0 30.0 35.0 25.0 37.0 17 33.66983913708805 35.0 33.0 35.0 30.0 37.0 18 31.96793938715336 35.0 30.0 35.0 25.0 37.0 19 31.971597058933302 35.0 30.0 35.0 25.0 37.0 20 32.5292053894674 35.0 32.0 35.0 26.0 37.0 21 34.13294517224648 35.0 32.0 39.0 27.0 39.0 22 34.88881424252603 37.0 33.0 39.0 29.0 39.0 23 35.58836263203075 37.0 34.0 39.0 30.0 39.0 24 36.148471615720524 37.0 35.0 39.0 32.0 39.0 25 36.32486097114918 37.0 35.0 39.0 32.0 39.0 26 37.61213749860038 39.0 36.0 40.0 33.0 41.0 27 37.81696711827716 39.0 37.0 40.0 33.0 41.0 28 38.045608927705 39.0 37.0 40.0 34.0 41.0 29 38.20292613742395 40.0 37.0 40.0 35.0 41.0 30 38.25801515321166 40.0 37.0 40.0 35.0 41.0 31 38.50688612697346 40.0 38.0 40.0 35.0 41.0 32 38.5882879856679 40.0 38.0 40.0 36.0 41.0 33 38.59399843242638 40.0 38.0 40.0 36.0 41.0 34 38.47814727727391 40.0 38.0 40.0 35.0 41.0 35 38.49766730116075 40.0 38.0 40.0 35.0 41.0 36 38.45978427201135 40.0 38.0 40.0 35.0 41.0 37 38.256783488224535 40.0 38.0 40.0 35.0 41.0 38 38.23759937297055 40.0 37.0 40.0 35.0 41.0 39 38.288582838801176 40.0 38.0 40.0 35.0 41.0 40 38.32064345164782 40.0 38.0 40.0 35.0 41.0 41 38.34031276826037 40.0 38.0 40.0 35.0 41.0 42 38.34497816593886 40.0 38.0 40.0 35.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07568867312724592 1101 2 0.817404618417541 1101 3 0.600160349753196 1101 4 -0.15046724864361494 1101 5 -0.3450917574203558 1101 6 -0.08291571414635968 1101 7 0.15979047651299538 1101 8 -0.05568738196498657 1101 9 -0.06182441713236386 1101 10 0.3057366107624233 1101 11 0.1525133471803315 1101 12 -0.3063032465459301 1101 13 0.1941632781303042 1101 14 0.9709971597067018 1101 15 -0.053103734243897804 1101 16 -0.7364021759947015 1101 17 -0.6089229354632764 1101 18 0.6851739179758738 1101 19 -0.21364560925670872 1101 20 -0.2685766803193914 1101 21 -0.8670881674247326 1101 22 -0.148753929563874 1101 23 -0.9435290970203525 1101 24 -1.1335149731325842 1101 25 -0.02045250123735798 1101 26 0.10370871335162946 1101 27 0.4156740870469946 1101 28 0.21813890718409112 1101 29 0.5859433451292588 1101 30 0.5628744778179353 1101 31 0.7618635279770345 1101 32 0.3711689628183805 1101 33 0.3253288618554535 1101 34 0.737596567930396 1101 35 0.8967582899795303 1101 36 0.4060895406855636 1101 37 0.2504208329505815 1101 38 0.05200542541351183 1101 39 0.7573934995333218 1101 40 0.8205647279063655 1101 41 0.579234403325593 1101 42 0.3919444597936206 1102 1 0.5849792136677863 1102 2 -0.2113115977986766 1102 3 0.24374143083427668 1102 4 0.8292624810861113 1102 5 1.2283879723093705 1102 6 0.9137059074752614 1102 7 0.9520202062427217 1102 8 1.2078261315485292 1102 9 0.5302363936784502 1102 10 0.8927298540056654 1102 11 0.837479563396549 1102 12 0.8921764831837962 1102 13 -0.14451915430212736 1102 14 -0.5822122997527579 1102 15 -1.0024280585682206 1102 16 -1.7490710949136208 1102 17 -1.6258148273551711 1102 18 0.3219982423001966 1102 19 -1.3090847984458982 1102 20 -0.040536139778851066 1102 21 -0.6905678971544589 1102 22 -0.8007809565908985 1102 23 0.3005587408174861 1102 24 -1.2416230812406894 1102 25 -1.4098106093454632 1102 26 -0.03480480016188636 1102 27 -0.9652380751151668 1102 28 -0.16277325497807027 1102 29 -0.5466580062220956 1102 30 -0.49709173839828225 1102 31 -0.17648106661756202 1102 32 0.2630608547102753 1102 33 0.11755859158517978 1102 34 0.41073845982229074 1102 35 0.32834612781736894 1102 36 0.6037246758207004 1102 37 0.22423840051814636 1102 38 0.055383803791890784 1102 39 -0.03483623019640447 1102 40 -0.8576447315530942 1102 41 -0.4435696507284632 1102 42 -0.28542040507124256 1103 1 -0.6042099755213997 1103 2 0.7346343481472672 1103 3 0.13563332272617146 1103 4 -0.522088870265236 1103 5 0.7148744587958618 1103 6 0.07586806963742276 1103 7 -0.23716898294646427 1103 8 -1.332714408992011 1103 9 -0.523817660375606 1103 10 -0.16132420004839076 1103 11 -0.4598177339007492 1103 12 -0.405120814113495 1103 13 0.4771024673194937 1103 14 -0.12275284029329825 1103 15 0.4840584279182636 1103 16 0.5482262023836775 1103 17 0.6985094969691588 1103 18 0.9976739179758738 1103 19 -0.03881452817562803 1103 20 0.04054494130222963 1103 21 0.22835102176445332 1103 22 0.037056881246940065 1103 23 0.1113695516282931 1103 24 0.40702556740795615 1103 25 -0.8962970958319545 1103 26 0.1814114160543312 1103 27 -0.3706434805205703 1103 28 -0.1087192009240141 1103 29 -0.27638773595182187 1103 30 0.3948001534936125 1103 31 0.1748702847337924 1103 32 -0.5207229290735143 1103 33 -0.017576543549949974 1103 34 -0.10277505369122508 1103 35 -0.18516738569614688 1103 36 -0.47735640526038026 1103 37 -0.5325183562386044 1103 38 -0.836508088100004 1103 39 -1.1159173112774923 1103 40 -0.992779866688231 1103 41 -1.6868128939717053 1103 42 -1.7448798645307022 1104 1 0.39579002447860034 1104 2 0.2751748886878147 1104 3 0.4059035929964381 1104 4 0.288721940545571 1104 5 0.9310906750120793 1104 6 0.48127347504282625 1104 7 0.4925607467832691 1104 8 0.39701532073771517 1104 9 0.7194255828676361 1104 10 0.6494866107624233 1104 11 0.1347768606938473 1104 12 0.5137981048054243 1104 13 0.15277814299516734 1104 14 -0.3930231105635684 1104 15 0.6191935630534005 1104 16 1.386064040221516 1104 17 -0.571760773301115 1104 18 0.673349593651551 1104 19 -0.2550307443918456 1104 20 -0.44594154518425455 1104 21 -1.2311084376950063 1104 22 -2.016997172807116 1104 23 -1.0778196375608928 1104 24 -1.1064879461055597 1104 25 -0.35575655529141414 1104 26 -0.4131831785402653 1104 27 -0.34361645349354575 1104 28 -6.110928159088758E-4 1104 29 0.2641528045887185 1104 30 0.800205558899016 1104 31 0.1748702847337924 1104 32 0.8036013952508156 1104 33 0.6851261591527518 1104 34 0.3837114327952591 1104 35 0.4634812629525058 1104 36 0.44156251365853905 1104 37 0.5755897518695008 1104 38 -0.025697277289189913 1104 39 -0.35916055452073437 1104 40 0.3585714846631234 1104 41 0.2591330519742385 1104 42 0.6605255408747013 1105 1 -0.03889466020608978 1105 2 -0.7728731593602376 1105 3 0.023771460864310257 1105 4 0.21064386246749933 1105 5 -1.1957862018647987 1105 6 -0.06902682525747394 1105 7 -0.6388206345981189 1105 8 0.41653484025723486 1105 9 -0.0027966393545852952 1105 10 0.01059772187353758 1105 11 -0.3787366528196685 1105 12 -0.03199769099037297 1105 13 -0.23986449964747436 1105 14 -0.09497506251551968 1105 15 -0.1781037342438978 1105 16 -0.7745966204391443 1105 17 0.15496595342560937 1105 18 -1.1412149709130155 1105 19 -0.7865622759233766 1105 20 -0.5532989025416128 1105 21 -0.4608381674247326 1105 22 -0.523753929563874 1105 23 -0.7595013192425739 1105 24 -0.43907052868814134 1105 25 -1.1072580567929151 1105 26 -1.2157357310928134 1105 27 -0.3030759129530054 1105 28 -0.306166648371466 1105 29 0.2491377895737017 1105 30 0.44134670004015675 1105 31 0.08478019464369879 1105 32 -0.10105325940384091 1105 33 -0.2510600270334322 1105 34 0.0744021234859531 1105 35 0.1849527344239732 1105 36 0.41303398513000644 1105 37 -0.06555138927163995 1105 38 -0.017439019030931036 1105 39 0.8615601661999861 1105 40 0.7684813945730298 1105 41 0.8327066255478144 1105 42 0.9405555709047349 1106 1 0.09443867312724308 1106 2 -0.8450953815824604 1106 3 -0.9873396502468026 1106 4 0.049532751356387905 1106 5 -0.676341757420353 1106 6 -1.0829157141463597 1106 7 -0.6027095234870075 1106 8 0.3693126180350106 1106 9 -0.20557441713236102 1106 10 0.12448661076242473 1106 11 -0.17873665281966566 1106 12 -0.41255324654593295 1106 13 -0.062086721869697215 1106 14 0.5272471597067003 1106 15 0.5718962657561022 1106 16 0.7948478240052985 1106 17 0.16607706453672222 1106 18 -0.22732608202412763 1106 19 0.3551043907432927 1106 20 1.0439233196806086 1106 21 0.564161832575266 1106 22 0.7512460704361246 1106 23 -1.1122790970203553 1106 24 -0.8835149731325842 1106 25 -0.6267025012373608 1106 26 -0.8212912866483677 1106 27 -0.6030759129530026 1106 28 0.04938890718408828 1106 29 0.07969334512925741 1106 30 -0.2558755221820661 1106 31 0.043113527977034494 1106 32 -0.6288310371816195 1106 33 -0.43717113814454933 1106 34 -0.4061534320696012 1106 35 0.04050828997952749 1106 36 0.36858954068556216 1106 37 0.6066708329505843 1106 38 0.6270054254135147 1106 39 0.11989349953331896 1106 40 -0.023185272093634524 1106 41 -0.5895155966744099 1106 42 -0.02055554020638084 1107 1 -0.21270418401561386 1107 2 0.09061890413182994 1107 3 -0.808768221675372 1107 4 -0.35761010578647046 1107 5 0.3272296711510734 1107 6 -0.22220142843207213 1107 7 -0.26342380920129216 1107 8 0.29431261803501485 1107 9 0.20513986858192368 1107 10 0.1923437536195678 1107 11 0.6212633471803315 1107 12 0.5053038963112115 1107 13 0.2021989924160188 1107 14 0.16296144542098645 1107 15 0.2933248371846737 1107 16 1.1127049668624416 1107 17 -0.076780078320418 1107 18 -1.11661179630984 1107 19 1.165818676457576 1107 20 1.2189233196806057 1107 21 0.09630468971812434 1107 22 -0.802325358135306 1107 23 -0.8515648113060692 1107 24 -0.6478006874182967 1107 25 0.21258321304835448 1107 26 0.4215658562087725 1107 27 0.796924087046996 1107 28 0.7422460500412313 1107 29 0.37612191655782823 1107 30 -0.13801837932491878 1107 31 0.10382781369131777 1107 32 0.14259753424695276 1107 33 0.27354314756973963 1107 34 0.2617037107875362 1107 35 0.49050828997953033 1107 36 0.068589540685565 1107 37 -0.4861863099065644 1107 38 -0.29442314601505615 1107 39 -0.39082078618096716 1107 40 -0.7696138435222082 1107 41 -0.21808702524583623 1107 42 -0.44912696877781144 1108 1 -0.4555613268727541 1108 2 0.21543093420701354 1108 3 0.2152919287005659 1108 4 -0.16362514338045742 1108 5 -0.6066049153150956 1108 6 -0.47107360888320216 1108 7 -0.19876215506594974 1108 8 -0.7530558030176167 1108 9 -0.3332059960797338 1108 10 -0.218934441869159 1108 11 0.27915808402243414 1108 12 0.004552016611967247 1108 13 0.051071172867146686 1108 14 0.3509313702330168 1108 15 -0.1781037342438978 1108 16 0.906689929268456 1108 17 0.05555074874725108 1108 18 0.39241076008113396 1108 19 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1109 13 0.21814583626984074 1109 14 0.22608436900902618 1109 15 0.2056171959886619 1109 16 -1.8766638039016783 1109 17 -0.8036903773237398 1109 18 -1.258140035512497 1109 19 0.32196485585956935 1109 20 -0.06944877334264987 1109 21 -0.728280027889852 1109 22 -0.4249167202615496 1109 23 0.3464418332122037 1109 24 0.19206642221625003 1109 25 0.3727161034138078 1109 26 -0.4282680308344169 1109 27 -0.9658666106274225 1109 28 0.3017144885794423 1109 29 0.0605072986176296 1109 30 0.18424075688770358 1109 31 0.06927631867471007 1109 32 0.2083782651439634 1109 33 0.2017823502275462 1109 34 0.33280005630248866 1109 35 0.09515945277022553 1109 36 0.49184535463905377 1109 37 0.586903391090118 1109 38 0.5839821695995582 1109 39 0.5553586158123949 1109 40 0.5785589139528753 1109 41 0.12211231030233449 1109 42 0.25386306444478635 1110 1 0.02062914931772042 1110 2 -0.3760477625348386 1110 3 -0.2659110788182346 1110 4 -0.019514867691235338 1110 5 -0.025151281229881306 1110 6 -0.6126776189082648 1110 7 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0.7327007957711302 2211 6 0.4197438603217236 2211 7 0.17973728502363429 2211 8 0.48154666058820794 2211 9 0.3151702637186986 2211 10 -0.1803006232801323 2211 11 0.599986751435651 2211 12 0.05606377473066715 2211 13 -0.24400161548671662 2211 14 0.004906734174788596 2211 15 -0.42278458530772767 2211 16 -0.1705777079095938 2211 17 0.7979919581537445 2211 18 0.23171647116735983 2211 19 -0.843299864575858 2211 20 -0.19809795691513443 2211 21 -0.36509348657367013 2211 22 0.3539056449042093 2211 23 -0.7925982459565262 2211 24 0.41967651622911717 2211 25 0.48553154131583653 2211 26 0.6063682878197127 2211 27 0.6224560019406127 2211 28 0.4249208220777092 2211 29 -0.11020027189201897 2211 30 0.20848617994559504 2211 31 0.14151778329618026 2211 32 -0.18202252654332085 2211 33 -0.7823839041019909 2211 34 -0.5024300278142846 2211 35 -0.7648108589566434 2211 36 0.0430576257919455 2211 37 0.46518147124845655 2211 38 0.2727501062645743 2211 39 0.09914881868225933 2211 40 0.38904877045956 2211 41 0.6955907863043151 2211 42 0.4092316938361762 2212 1 0.43574302095333195 2212 2 0.7005567923305875 2212 3 0.3365733932314541 2212 4 0.2299675339650804 2212 5 0.6073538947535582 2212 6 0.5094755902014683 2212 7 0.48316004173038607 2212 8 0.10735609629588083 2212 9 0.5672516698241594 2212 10 0.4973126977189466 2212 11 0.4256111732672849 2212 12 0.4885337099758118 2212 13 0.005304582478128594 2212 14 -0.16623110116286455 2212 15 0.2784180048865359 2212 16 -0.6345000020816585 2212 17 -0.49479250068066705 2212 18 0.019413048410655165 2212 19 -0.6198956092567087 2212 20 0.28848853707191324 2212 21 0.14785748474918137 2212 22 -0.1867974078247414 2212 23 -0.4285834448464385 2212 24 0.062137200780462365 2212 25 -0.49735467515040455 2212 26 0.17436088726467602 2212 27 0.13170669574264338 2212 28 0.08634542892322372 2212 29 0.4786063886075169 2212 30 -0.1069624787038066 2212 31 -0.0818864720229655 2212 32 0.3059515715140293 2212 33 0.3226114705511023 2212 34 -0.24202299728699472 2212 35 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1.2994555968572001 2214 24 0.7848523738061886 2214 25 0.881970968150398 2214 26 0.6215658562087754 2214 27 0.27855674010822185 2214 28 0.7136746214698064 2214 29 0.2944892634966081 2214 30 -0.015569399733088574 2214 31 0.23444005858927852 2214 32 0.10586284036940441 2214 33 0.48986967818198224 2214 34 0.11068330262427395 2214 35 0.2660184940611643 2214 36 -0.06202270421239575 2214 37 0.0648340982567035 2214 38 -0.34340273785179676 2214 39 -0.5908207861809629 2214 40 -0.1328791496446584 2214 41 -0.40992375993971564 2214 42 -0.8899432953084201 2215 1 -0.015561326872756354 2215 2 -0.17509538158245874 2215 3 -0.577339650246806 2215 4 -0.660467248643613 2215 5 -0.7613417574203538 2215 6 -1.067915714146359 2215 7 -0.637709523487004 2215 8 -1.3656873819649888 2215 9 -0.8805744171323653 2215 10 -1.0105133892375768 2215 11 -1.0787366528196678 2215 12 -0.8375532465459301 2215 13 0.24791327813030506 2215 14 -0.32275284029329754 2215 15 -0.3181037342438984 2215 16 0.28984782400529596 2215 17 0.34607706453672193 2215 18 0.8976739179758724 2215 19 0.8801043907432913 2215 20 0.37892331968060944 2215 21 0.4791618325752651 2215 22 0.24624607043612912 2215 23 0.2627209029796447 2215 24 0.40648502686741494 2215 25 0.5782974987626446 2215 26 0.61870871335163 2215 27 0.23692408704699375 2215 28 0.2993889071840883 2215 29 -0.3153066548707386 2215 30 0.39912447781793503 2215 31 -0.12188647202296465 2215 32 -0.7288310371816209 2215 33 -0.33217113814454535 2215 34 -0.14115343206960063 2215 35 0.3705082899795329 2215 36 -0.39141045931443585 2215 37 -0.12332916704941965 2215 38 -0.22299457458648675 2215 39 0.4348934995333238 2215 40 -0.6181852720936334 2215 41 -0.8095155966744088 2215 42 -0.6405555402063783 2216 1 -0.4683818396932651 2216 2 -0.9181723046593788 2216 3 -0.7604165733237238 2216 4 -0.6440569922333594 2216 5 -0.5269827830613849 2216 6 -0.6566336628643086 2216 7 -1.3674531132305958 2216 8 -1.074918151195753 2216 9 -0.07544621200415946 2216 10 -0.8376928764170621 2216 11 -0.5838648579478729 2216 12 -0.3503737593664411 2216 13 -0.12234313212610459 2216 14 0.261862544322085 2216 15 -0.4216934778336423 2216 16 0.913437567595043 2216 17 0.5224873209469791 2216 18 0.2028021231040782 2216 19 0.726258236897138 2216 20 1.598410499167791 2216 21 2.1930079864214207 2216 22 1.739066583256637 2216 23 0.20631064656939202 2216 24 0.3280234884058757 2216 25 0.9803487808139266 2216 26 0.6325548671977828 2216 27 0.0687189588418633 2216 28 0.025029932825120227 2216 29 -0.06710152666560987 2216 30 -0.22959347090001359 2216 31 -0.4023992925357831 2216 32 -0.09036949872007938 2216 33 -0.2788378048112108 2216 34 -0.7119226628388375 2216 35 -0.5094917100204697 2216 36 -1.4032053311093051 2216 37 -1.1638419875622361 2216 38 -0.8870971386890503 2216 39 0.1564319610717817 2216 40 -0.3469032208115834 2216 41 -0.5946438018026114 2216 42 -0.18978630943714592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 7.0 27 39.0 28 98.0 29 168.0 30 337.0 31 518.0 32 932.0 33 1633.0 34 3150.0 35 5818.0 36 7620.0 37 4303.0 38 2170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 97.32392789161348 1.9184115253984249 0.5187922218489904 0.2388683611391035 2 0.43668122270742354 98.82431978501847 0.507595267420595 0.2314037248535065 3 0.5785093121337663 0.7837868099876834 0.6419587205613406 97.9957451573172 4 0.3247116784234688 0.3620348598514537 98.38017392602545 0.9330795356996231 5 0.31725888324873097 98.58913108390564 0.6009256494475963 0.4926843833980293 6 3.0679655133803605 0.2836561788526854 0.4105549957078341 96.23782331205912 7 97.87615243925198 1.422119368444627 0.3844574670598335 0.31727072524355193 8 2.9074758332400252 1.1831448512671219 95.45030418392864 0.4590751315642145 9 32.05807703792177 21.31233203941475 26.940877873992235 19.688713048671243 10 29.97051468667189 21.43843541223454 28.593289291979247 19.99776060911432 11 31.590340760646434 20.012689881685514 25.432015825028927 22.964953532639125 12 29.73164632553279 18.236106445713432 27.89907811741873 24.13316911133505 13 53.56276361464671 26.665671307528648 7.431600164234258 12.339964913590384 14 23.793528160340387 28.040906206845072 35.13604299630501 13.029522636509535 15 15.134550069047886 17.825551450005598 54.28283506886127 12.757063412085245 16 13.149447596297403 18.8750373245745 51.728127799343085 16.247387279785013 17 14.787444481767626 22.117717314223864 11.017803157541149 52.07703504646736 18 45.99245902863329 22.466121626161943 13.267629820435287 18.273789524769477 19 26.542495614198796 32.495987458474865 21.794632525848233 19.166884401478107 20 21.18463777852424 25.39096032545814 24.48773933490091 28.936662561116712 21 25.980666592020302 24.64822901504124 25.585040868883663 23.78606352405479 22 27.36162430485575 27.410144440712124 24.405628335759342 20.82260291867279 23 20.24409360653902 29.336020602396147 29.79882805210316 20.621057738961667 24 19.617064158548875 24.461613107901318 27.704997573993207 28.2163251595566 25 24.55865337961408 22.207292949651027 27.167543761430224 26.06650990930467 26 27.30190721457097 26.40988317844213 25.308849326316572 20.979360280670324 27 23.330720710633372 26.92867540029112 29.31362669353936 20.426977195536146 28 24.55118874332848 26.895084537005935 24.939349830179523 23.61437688948606 29 27.145149852573436 26.241928862016202 26.943604672862314 19.669316612548055 30 23.297129847348188 30.978240585227486 27.186205352144217 18.53842421528011 31 22.55524037025978 25.645715138847418 26.47058823529412 25.328456255598685 32 20.531482103534504 23.666629343485237 32.01209271078267 23.789795842197588 33 27.055574217146273 25.069980965177475 28.350688612697343 19.523756204978913 34 23.006009032209906 25.794050684880382 31.77695666778636 19.422983615123353 35 21.027880416526703 30.705781360803197 27.313104168999363 20.953234053670737 36 27.286977941999773 27.27578098757138 25.752995185309597 19.684245885119246 37 22.912701078639945 31.523159034076066 24.980405329750308 20.583734557533685 38 19.766356884260812 33.811070055611545 25.237935281603402 21.18463777852424 39 18.55777844132577 28.068080023887727 28.370409077336518 25.003732457449985 40 20.917472285469003 26.329737598447238 29.980217237131875 22.772572878951884 41 18.848206621132384 26.798044265293175 30.877467995371926 23.476281118202515 42 17.8000895789788 27.052851597491788 34.30128396536279 20.845774858166617 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.0 10 1.0 11 10.0 12 19.0 13 29.0 14 39.0 15 48.0 16 57.0 17 57.0 18 75.0 19 93.0 20 129.5 21 166.0 22 166.0 23 222.0 24 278.0 25 364.0 26 450.0 27 449.0 28 448.0 29 448.0 30 575.0 31 702.0 32 850.0 33 998.0 34 998.0 35 1025.0 36 1052.0 37 1171.0 38 1290.0 39 1467.5 40 1645.0 41 1645.0 42 1737.5 43 1830.0 44 1924.5 45 2019.0 46 2267.5 47 2516.0 48 2516.0 49 2663.0 50 2810.0 51 2808.5 52 2807.0 53 2807.0 54 2836.5 55 2866.0 56 2296.5 57 1727.0 58 1472.0 59 1217.0 60 1217.0 61 962.0 62 707.0 63 564.5 64 422.0 65 341.0 66 260.0 67 260.0 68 204.5 69 149.0 70 125.5 71 102.0 72 102.0 73 86.5 74 71.0 75 46.5 76 22.0 77 21.0 78 20.0 79 20.0 80 13.5 81 7.0 82 4.5 83 2.0 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0037323181427984923 6 0.0 7 0.007464636285596985 8 0.0 9 0.0037323181427984923 10 0.0 11 0.0 12 0.0 13 0.007464636285596985 14 0.0 15 0.0 16 0.0037323181427984923 17 0.0 18 0.02239390885679095 19 0.007464636285596985 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0037323181427984923 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0037323181427984923 40 0.007464636285596985 41 0.0 42 0.0037323181427984923 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 26793.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.78244317545627 #Duplication Level Percentage of deduplicated Percentage of total 1 73.05897327110735 38.56231105139402 2 12.58662141139867 13.287052588362632 3 5.550841465139301 8.789609226290448 4 2.4819686041578275 5.240174672489083 5 1.7677839060953189 4.665397678498115 6 1.1384528355253853 3.605419325943343 7 0.8061094611794655 2.9783898779531968 8 0.48083722245792676 2.03038106968238 9 0.3535567812190638 1.6795431642593215 >10 1.711214821100269 15.17187325047587 >50 0.04949794937066893 2.1050274325383493 >100 0.01414227124876255 1.8848206621132384 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GACTACGTTTCTAATGACGCTGAACGGCTTGCGGCGCACCTT 359 1.3399022132646587 No Hit GACTAGGTAGTCTCTACAGTCCGACGATCAGTCTAGAAAGGT 146 0.5449184488485799 No Hit GACTACGTTACTGTAACTTTTTTGTGTATGTACTTAGCTGTA 100 0.37323181427984925 No Hit GACTACGTCTTCGCTTCAGCTCCAGCCCAGGCTCCCAAAGGT 93 0.34710558728025975 No Hit GACTACGTTATTGTGAAGTAAAATGGTGAAGCTTATGAATTT 85 0.31724704213787186 No Hit GACTACGTCACCGCGACGATCATCGGACTGTAGAACTCTGAA 77 0.28738849699548386 Illumina DpnII expression Sequencing Primer (96% over 25bp) GACTACGTAAGGGATTTGAGGAAGCCTGCTCCTAGTACGAGA 75 0.2799238607098869 No Hit GACTACGTGGATCTGTAGGTGGTCATTCATTCACATGATGAA 68 0.2537976337102975 No Hit GACTACGTCCTTGGAAGGAATTCCTCCTGATCAGCAGAGACT 66 0.2463329974247005 No Hit GACTACGTTGGGGGTTCGACTGTAGAACTCTGAACCCCTATA 50 0.18661590713992462 No Hit GACTACGTGCCGCAGTCCGACGATCTTAGGACTACGTTTTTT 49 0.18288358899712612 No Hit GACTACGTCACCGATCCGGGGACTGTAGAACTCTGAACCCCT 46 0.17168663456873062 Illumina DpnII expression Adapter 1 (95% over 21bp) GACTACGTAGTGATTTTAGGCTACAATTGGGAAGACAGTAAT 39 0.1455604075691412 No Hit GACTACGTTTTCAGTCCGACGATCGGTGGACTGTAGAACTCT 39 0.1455604075691412 No Hit GACTACGTTGCTGTTTTGCCGGCGCACAAGCACGATCGGCTC 38 0.1418280894263427 No Hit GACTACGTCCTGGTTTCAGTTCTGGTTTCTTCCCTCCTGACC 38 0.1418280894263427 No Hit GACTACGTGCGTGATTAAGGGGGATACAAATTAGAAAGAACT 37 0.13809577128354422 No Hit GACTACGTGTCCGAGGAGCGGACTTGTAAGGACCGCCGCTTT 36 0.13436345314074571 No Hit GACTACGTTGCGGATTGGGCGGATTGAGGATGTCACCCCCAT 36 0.13436345314074571 No Hit GACTACGTTTAGGATATGATGAACGTCTCTGAACCTCAAAAA 36 0.13436345314074571 No Hit GACTAGGGGTACGAACCCCTATAGTGAGTCGTATTACCGGCC 35 0.1306311349979472 No Hit GACTACGTTGCTGGATAGATAGCCACCTATATAGTATAGCTT 34 0.12689881685514875 No Hit GACTACGTGTGTGTTTCAGTCTCGACGGGCTAGCACACATCT 33 0.12316649871235025 No Hit GACTACGTGAGGATCTCGGGACTACGTTTTTTTTTTTTTTTT 33 0.12316649871235025 No Hit GACTACGTTTTGAGTCCGACGATCCCCAGACTGTAGAACTCT 33 0.12316649871235025 No Hit GACTACGTGCTGACCGGCGAGACCCGTCGGGCCTTCAGCCTG 32 0.11943418056955175 No Hit GACTACGTTGGCGATCCGTCGACTGTAGAACTCTGAACCCCT 32 0.11943418056955175 No Hit GACTACGTATGAGCTTCGACAGAATTGAGTACATGCCTTTCT 31 0.11570186242675325 No Hit GACCACGGGGCTGCGGAAGATGCTGGGCCCGTCGGCCATGAC 31 0.11570186242675325 No Hit GACTACGTATAGATTTCAAAGGGAACTGAGGCCCAATGAAGA 30 0.11196954428395475 No Hit GACTACGTTCGCGTGCAGAGGGAGGAGAATGAGGTTCTGTGT 30 0.11196954428395475 No Hit GACTACGTATTAGTTTCACTAGATCGTGTTATGGACGATGAC 30 0.11196954428395475 No Hit GACTACGTCCTTGGGAAGGAATTCCTCCTGATCAGCAGAGAC 29 0.10823722614115629 No Hit GACTACGTTGTGGTTTGTCCCAGTAGCAGGGACACCAAGGCC 27 0.10077258985555929 No Hit GACTACGTAGGTGTTCCAAGGGCCGGAAGTTTGAGCGCGCCA 27 0.10077258985555929 No Hit GACTACGTCAATGTTTGGGTCTTCCGAGGCCAAGAGCTTGCC 27 0.10077258985555929 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0037323181427984923 0.0 0.0 13 0.0 0.0 0.007464636285596985 0.0 0.0 14 0.0 0.0 0.03732318142798492 0.0 0.0 15 0.0 0.0 0.05225245399917889 0.0 0.0 16 0.0 0.0 0.05225245399917889 0.0 0.0 17 0.0 0.0 0.06344940842757436 0.0 0.0 18 0.0 0.0 0.0895756354271638 0.0 0.0 19 0.0 0.0 0.10077258985555929 0.0 0.0 20 0.0 0.0 0.29112081513828236 0.0 0.0 21 0.0 0.0 0.9442764901280185 0.0 0.0 22 0.0 0.0 1.395886985406636 0.0 0.0 23 0.0 0.0 1.418280894263427 0.0 0.0 24 0.0 0.0 1.4929272571193968 0.0 0.0 25 0.0 0.0 1.6795431642593215 0.0 0.0 26 0.0 0.0 1.7504572089724928 0.0 0.0 27 0.0 0.0 1.8101742992572687 0.0 0.0 28 0.0 0.0 1.9221438435412235 0.0 0.0 29 0.0 0.0 2.161012204680327 0.0 0.0 30 0.0 0.0 2.272981748964282 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGACGA 30 9.6687745E-6 36.000004 13 ACGTTGA 30 9.6687745E-6 36.000004 5 CGTAGCT 30 9.6687745E-6 36.000004 6 TACGTCT 120 0.0 36.000004 4 CTACGTG 845 0.0 36.000004 3 CTACGTC 480 0.0 36.000004 3 CGTGTGT 30 9.6687745E-6 36.000004 6 CGTGCCG 30 9.6687745E-6 36.000004 6 AATACGA 30 9.6687745E-6 36.000004 13 CGTGATG 30 9.6687745E-6 36.000004 6 CGTGAGC 30 9.6687745E-6 36.000004 6 ACGTGTG 60 1.8189894E-12 36.000004 5 ACGTGGC 60 1.8189894E-12 36.000004 5 ACTACAT 30 9.6687745E-6 36.000004 2 AGGGGTA 55 1.4551915E-11 36.0 5 CGCGACG 20 0.0020706085 36.0 12 CGTTTAG 20 0.0020706085 36.0 6 CGCTGAA 45 3.1668606E-9 36.0 18 ACGCTGA 45 3.1668606E-9 36.0 17 CCGGAAG 20 0.0020706085 36.0 23 >>END_MODULE