FastQCFastQC Report
Thu 26 May 2016
SRR2099224_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099224_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences629374
Sequences flagged as poor quality0
Sequence length42
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACGACCTGCCTTTCATGCGCCACCCGCGCGCACGGCCCGG61420.9758903291206819No Hit
TGACGATCCGGGGGGCAGGACCGGTTCGCAGGGACAGTGCAC47000.7467737783893201No Hit
TGACGACGAAGTCAGCGCTTTGCGCCCCGGCTGCGCACCGTC31400.498908439179248No Hit
TGACGATCTCGAGTGGGCAGGTGGGGGAGCACCCTTTCAGAA28710.45616755696930605No Hit
TGACGAACTTATAGCGCGTCGACCAGCTGAAGCTTGTTCTTC22490.35733919736118747No Hit
TGACAATCCAAGGGTTATCGGTTGGAGGCAGTGCATTGCCAT21750.34558148255250454No Hit
TGACGATCAGCGGTTCACGGGTCAAATGGGGGATTCTTGAAG21670.3443103782488631No Hit
TGACGATGGGATGACCGTCCGGCGCCGGCAACCGATGCCGTG18380.29203621376161076No Hit
TGACGACGAAGTGCAGCGCTTTGCGCCCCGGCTGCGCACCGT16420.26089415832239654No Hit
TGACGATCTCCCGGAGCCAGGGATCCCTGGAAAAATGGCTGA15320.24341647414732734No Hit
AAACGATCCCAAGCCGATTGCATGGGGCCTGGTAGTCGTCGC13300.21132109048038208No Hit
TGACCTTCATGCGGAGCAAATATCGCTGCCTTCGAAAACGTA13220.2100499861767407No Hit
TGACGATGGGATACCGTCCGGCGCCGGCAACCGATGCCGTGC12600.2001989278235199No Hit
TGACGATCTCGAGGTGGGCAGGTGGGGGAGCACCCTTTCAGA12300.19543228668486462No Hit
TGCTGATCTAGTGATGTTGGGTTAAGTCCCGCAACGAGCGCA12100.19225452592576117No Hit
AGACGACCAGTCCTTATTCTTCTAACTACCAGTCCAAAACTA9450.15014919586763992No Hit
TGACGGTCCGAGGTCCGCGACCGCATCTAGCTTCAGTGCGGC9060.1439525623873881No Hit
TGACGATCCGGGGGGGCAGGACCGGTTCGCAGGGACAGTGCA8800.13982147340055356No Hit
TGACGATCACGCCAGTCCGACGATCGCTGTGACGATCTTTTT8650.13743815283122596No Hit
TGACGATCGGAGACCAAAAATAATGAACTTAAAACAAAAATA8000.1271104303641396No Hit
TGACGATCACAGGTGACGATCTTTTTTTTTTTTTTTTTTTTT7970.1266337662502741No Hit
TGACGAGAAGCGCGCGCGAAGCGGCTGCTGCTCGCGAAGAAA7480.11884825239047052No Hit
TGACGATCGTATGGCCGCTGCCGTCGGGCAGCATCACGTCGA7470.11868936435251536No Hit
TGAAGATAGACTGAAAGTTCTTTGTGATGATTGCATTCAAGT7030.11169829068248768No Hit
TGACGAAGTGAGGCGGCCTGGTACGCCCGAATGCTCGGCATG6900.10963274618907042No Hit
TGACGATCGGTGTTTCGATTCGACTTCGGCGGCCAATATCTC6520.10359500074677377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTT200.00209671936.033759
CACGACA309.958394E-636.033758
CTTGCGC200.00209671936.033758
CCGAGAC200.00209671936.033758
CCGCGGA309.958394E-636.033758
GTACGCG200.00209671936.033759
CTTACGC200.00209671936.033758
TCGGGTA750.036.030887
TCTACCG200.002097538236.030887
TCGTACC1150.036.030887
TCGCGTA404.836329E-836.030887
TCCGCGG1750.036.0308767
AATCGGC356.9254384E-736.0308765
ATATCGT502.382876E-1036.0280154
AGCGTCC404.870344E-836.00222410
ATAGCGC2350.036.00222411
TACGGGG200.00210574536.00222410
TATAGCG2350.036.00222410
TAGTGCG404.870344E-836.00222410
TACGTTG200.00210574536.00222410
Produced by FastQC (version 0.11.5)