FastQCFastQC Report
Thu 26 May 2016
SRR2099223_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099223_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19228
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACGACCTGCCTTTCATGCGCCACCCGCGCGCACGGCCCGG1600.8321198252548367No Hit
TGACGATCCCCAGTCGCGGAGGCACGTTACCGCGCACAACCC960.499271895152902No Hit
TGACGACGAAGTCAGCGCTTTGCGCCCCGGCTGCGCACCGTC920.4784688995215311No Hit
TGACGATCCGGGGGGCAGGACCGGTTCGCAGGGACAGTGCAC870.45246515498231743No Hit
TGACGATCCAAAGTAGCCTAGAGCGCCAGGGGCGGAAATTCG810.4212606615352611No Hit
TGACGATCGGGCGTTTCTATTGAAACTGGGTTAAATTGACAT800.41605991262741837No Hit
TGACGATCGCGGGTTTTGACGATCTTCGGACTGTAGAACTCT770.40045766590389015Illumina DpnII expression Sequencing Primer (95% over 22bp)
TGACGATCTCGAGTGGGCAGGTGGGGGAGCACCCTTTCAGAA600.31204493447056375No Hit
TGACGATCTTCGGTTAAAACTTGATGTTTTGATTTAGCTTCG580.3016434366548783No Hit
TGACGATCCCACAATACGACTCACTATAGCTATTGAAGGAAG560.2912419388391928No Hit
TGACGAACTTATAGCGCGTCGACCAGCTGAAGCTTGTTCTTC540.28084044102350736No Hit
TGACGATCTTCTGTTGACTAGGCGCATAGTCGCCATTCACAG470.24443519866860827No Hit
TGACGATCTAAGGTTTAAATTGACATCTTCATAAGGGGTTGG440.2288329519450801No Hit
TGACGATCCTGGGTAAGGAATTGGTGAAGTAAAATGGTGAAG420.21843145412939463No Hit
TGACGATCTGGTGTAAAAGAAGCGGATTTAATTCTGCATTTA410.2132307052215519No Hit
TGACGATCACTCAGTCCGACGATCCGTCTGACTGTAGAACTC410.2132307052215519No Hit
TGACGATCAGCGGTTCACGGGTCAAATGGGGGATTCTTGAAG400.20802995631370919No Hit
TGACGATCGGAAGTAGCCTAGAGCGCCAGGGGCGGAAATTCG400.20802995631370919No Hit
TGACGATGGGATGACCGTCCGGCGCCGGCAACCGATGCCGTG380.1976284584980237No Hit
TGACGACGAAGTGCAGCGCTTTGCGCCCCGGCTGCGCACCGT360.18722696068233824No Hit
TGACAATCCAAGGGTTATCGGTTGGAGGCAGTGCATTGCCAT350.18202621177449552No Hit
TGACGATCATGAGTTGACTAGGCGCATAGTCGCCATTCACAG350.18202621177449552No Hit
TGACGATCCGAAGTTTAAAGAGGCATCTATCACATAAGGCAT330.17162471395881007No Hit
TGACGATCAGATGTCCGACGATCGTGTTGACGATCGTCGGAC330.17162471395881007No Hit
AAACGATCCCAAGCCGATTGCATGGGGCCTGGTAGTCGTCGC320.16642396505096732No Hit
TGACGATCTCGAGGTGGGCAGGTGGGGGAGCACCCTTTCAGA310.1612232161431246No Hit
TGACGATCTTTAGTTTTAATAGCGTTTAAATTGACATCTTCA310.1612232161431246No Hit
TGACGATCAGTCGCTACAGTCCGACGATCGGACTGTAGAACT300.15602246723528188No Hit
TGACAATCGTTGAATACGACTCACTATAGGGAGTCGTATTAC300.15602246723528188No Hit
TGACGATCTTTAGTAAAAGAAGCGGATTTAATTCTGCATTTA300.15602246723528188No Hit
TGACGATCCCGTGCTAAGACAGCTAAGAAAGCTCCAATTGGT280.1456209694195964No Hit
TGACGATCAGAAGTATAGCTTCCCATCTTCTTTGAGAGTTGT280.1456209694195964No Hit
TGACAATCGAGTGTTTTGGATGTTTGGTGCCCTGAGTAGTTT270.14042022051175368No Hit
TGACGATCTCGGGTAAAAGAAGCGGATTTAATTCTGCATTTA270.14042022051175368No Hit
TGACGATCTCCCGGAGCCAGGGATCCCTGGAAAAATGGCTGA260.13521947160391096No Hit
TGCTGATCTAGTGATGTTGGGTTAAGTCCCGCAACGAGCGCA260.13521947160391096No Hit
TGACGGTCCGAGGTCCGCGACCGCATCTAGCTTCAGTGCGGC250.13001872269606823No Hit
TGACGATCACAAATTTCGCAGATGATATCCCGATGCTGACGG250.13001872269606823No Hit
TGACGATCAAGTGTTCAGGATCTTCCGACGACATTGATTGCT240.1248179737882255No Hit
TGACGATCTGACGCGACGATCTGATTGACGGACTGTAGAACT230.11961722488038277No Hit
TGACGATCATGCGCTTAGATATTTTAAAGAGGCATCTATCAC230.11961722488038277No Hit
TGACGATCGGCGGTACCGACCGCCGGAAGGGATCACATTATG230.11961722488038277No Hit
TGACGATCCGTCGTTTGGTGCCCTGAGTAGTTTCCGGGGCGT220.11441647597254005No Hit
TGACGATCTTTCGATGCGGGACATGAAAAAACAGTGCTTCGG220.11441647597254005No Hit
TGACGATCATGAGTTTCTGAGTTGCCTAGAGTATTGACAATT220.11441647597254005No Hit
TGACGATCAACTGTTGACTAGGCGCATAGTCGCCATTCACAG220.11441647597254005No Hit
TGACGATCCGGAGTTTCAGTCTCGACGGGCTAGCACACATCT210.10921572706469732No Hit
TGACGATCCATCGGTCCGACGATCCCGATGACGATCTTTAGA210.10921572706469732No Hit
TGACGATCCAGTGCCAAGGGAATAAGGAACGTTCCATATCGC210.10921572706469732No Hit
TGACGATCAGTAAATTAGCTGGGCGTGGTTGTGCGTGCCTGT210.10921572706469732No Hit
TGACGATGGGATACCGTCCGGCGCCGGCAACCGATGCCGTGC210.10921572706469732No Hit
TGACGATCCGGCGGATCGATTCCAGATGAGGATCCACAAGCG210.10921572706469732No Hit
TGACGATCGAGAGAAGCGGATTTAATTCTGCATTTAATTGAT210.10921572706469732No Hit
TGACGATCAGCCGATTAGCAAGTCTAGCTAATCTAGCTCTAC210.10921572706469732No Hit
TGACGATCTAAGAGTCCGACGATCGGCCTGACGATCTTCGGA210.10921572706469732No Hit
TGACGATCCATCGTCTCGACGGGCTAGCACACATCTGGTTGA200.10401497815685459No Hit
TGACGATCTCTAATTTCACTAATTCCAATACTTATTATTATT200.10401497815685459No Hit
TGACCTTCATGCGGAGCAAATATCGCTGCCTTCGAAAACGTA200.10401497815685459No Hit
TGACGAGAAGCGCGCGCGAAGCGGCTGCTGCTCGCGAAGAAA200.10401497815685459No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTG251.3797356E-436.075727
GAGTCCG356.408027E-736.0757212
TCAGCTG251.3797356E-436.075727
TCCCCAG251.3797356E-436.075727
GCGACGA309.388741E-636.07571813
CCTGCCT200.002034804336.0757187
TTTCATG200.002034804336.07571813
GTTTTGA200.002034804336.07571813
TCCGGGG200.002034804336.0757187
GGCTTAG200.002034804336.07571812
TCCAAAG200.002034804336.0757187
TCTTTGG200.002034804336.0757187
TGGCTTA200.002034804336.07571811
GATCGAG356.5414315E-735.9817735
GATCTTA251.4012025E-435.9817735
GATCCGA251.4012025E-435.9817735
GATCCCT251.4012025E-435.9817735
GATCCCC502.1282176E-1035.9817735
GATCTCG502.1282176E-1035.9817735
GATCCAG251.4012025E-435.9817735
Produced by FastQC (version 0.11.5)