##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099223_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19228 Sequences flagged as poor quality 0 Sequence length 42 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.252548366964845 37.0 35.0 37.0 33.0 37.0 2 35.54165799875182 37.0 35.0 37.0 33.0 37.0 3 34.60562721031829 35.0 35.0 37.0 32.0 37.0 4 35.577283128770546 37.0 35.0 37.0 35.0 37.0 5 37.399729561056795 39.0 37.0 39.0 35.0 39.0 6 34.89359267734554 37.0 34.0 37.0 31.0 39.0 7 36.55429581859788 37.0 37.0 39.0 34.0 39.0 8 37.62185354691076 39.0 37.0 39.0 35.0 39.0 9 31.760609527772 33.0 31.0 34.0 30.0 34.0 10 32.04046182650302 33.0 31.0 34.0 30.0 34.0 11 32.13334720199709 34.0 31.0 34.0 30.0 34.0 12 35.616184730601205 37.0 35.0 37.0 35.0 37.0 13 31.0720823798627 31.0 30.0 31.0 30.0 34.0 14 30.64192843769503 31.0 30.0 31.0 27.0 34.0 15 30.007697108383606 31.0 30.0 31.0 26.0 34.0 16 33.07104223008113 35.0 32.0 37.0 28.0 37.0 17 34.0990742666944 35.0 33.0 37.0 30.0 37.0 18 31.97789681714167 35.0 30.0 37.0 25.0 37.0 19 31.879862700228834 35.0 30.0 37.0 23.0 37.0 20 32.03068441855627 35.0 30.0 37.0 25.0 37.0 21 33.02225920532557 35.0 30.0 39.0 25.0 39.0 22 33.33877678385687 35.0 30.0 39.0 25.0 39.0 23 34.0788433534429 35.0 32.0 39.0 27.0 39.0 24 34.84605783232786 37.0 33.0 39.0 27.0 39.0 25 34.88912003328479 37.0 33.0 39.0 27.0 39.0 26 36.249167880174745 38.0 34.0 40.0 30.0 41.0 27 36.5679737882255 38.0 35.0 40.0 31.0 41.0 28 37.11446848346162 38.0 36.0 40.0 32.0 41.0 29 37.33518826711046 38.0 36.0 40.0 32.0 41.0 30 37.59101310588725 39.0 36.0 40.0 33.0 41.0 31 37.942999791970045 39.0 37.0 40.0 34.0 41.0 32 38.22254004576659 40.0 37.0 40.0 35.0 41.0 33 38.32697108383607 40.0 37.0 40.0 35.0 41.0 34 38.45938215102975 40.0 37.0 40.0 35.0 41.0 35 38.56178489702517 40.0 38.0 40.0 35.0 41.0 36 38.65638651965883 40.0 38.0 40.0 35.0 41.0 37 38.577803203661325 40.0 38.0 40.0 35.0 41.0 38 38.59605783232786 40.0 38.0 40.0 35.0 41.0 39 38.606251300187225 40.0 38.0 40.0 35.0 41.0 40 38.6441647597254 40.0 38.0 40.0 35.0 41.0 41 38.550447264406074 40.0 38.0 40.0 35.0 41.0 42 38.463386727688786 40.0 38.0 40.0 35.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11071457351122405 1101 2 0.7181398681920186 1101 3 -0.46659458434216106 1101 4 0.5607927760563527 1101 5 0.6570752991735205 1101 6 0.9542383946640953 1101 7 1.1018050656933909 1101 8 0.6911233037577773 1101 9 -0.002202619737932565 1101 10 0.45618585875077144 1101 11 -0.5178869197495573 1101 12 0.49928641259828765 1101 13 0.48132216521880267 1101 14 0.1270618017091607 1101 15 0.6147123180024074 1101 16 0.6794434998157044 1101 17 -0.4129135057805371 1101 18 -0.0301700396351805 1101 19 1.6776467293053585 1101 20 1.9307152012731734 1101 21 2.043862990971448 1101 22 0.8248654092892167 1101 23 -0.37225104208081206 1101 24 -1.7853941079272104 1101 25 0.19120508921014334 1101 26 -0.28766851621182354 1101 27 0.6012395107909967 1101 28 -0.353251663284496 1101 29 0.14840968588299575 1101 30 -0.14933961072819812 1101 31 0.7121014037895748 1101 32 -0.9499148114769227 1101 33 0.44821869707584483 1101 34 0.5934241784959369 1101 35 0.5411985392677963 1101 36 0.4247027774282017 1101 37 1.21334594710261 1101 38 1.2707542894280124 1101 39 1.255240191045793 1101 40 1.1367144819853507 1101 41 1.019218708990735 1101 42 0.43795343805694387 1102 1 1.0847405475372014 1102 2 0.923767573819724 1102 3 0.3018036840561109 1102 4 0.6192343344979108 1102 5 0.37785451995274144 1102 6 -0.14532870490300098 1102 7 0.6624111262994532 1102 8 -0.0989199862855088 1102 9 1.1341610166257041 1102 10 1.0059694085343196 1102 11 1.1357927339300957 1102 12 0.7958232091350865 1102 13 0.3666035505001872 1102 14 0.9777111523585127 1102 15 0.787872491162581 1102 16 0.5755473959195996 1102 17 -0.9064199992870243 1102 18 1.3529468434817034 1102 19 1.662495214153843 1102 20 -1.1558648853069116 1102 21 -3.241851294742837 1102 22 -0.7855242011003938 1102 23 -0.05623372606349619 1102 24 0.5046491821160757 1102 25 -1.2330373350322859 1102 26 -1.1404823690256762 1102 27 -0.29269988314839424 1102 28 -1.8424291524619818 1102 29 -1.0680405305672167 1102 30 -0.8895993509879361 1102 31 -0.7814050897169125 1102 32 -0.4585728201349326 1102 33 -0.6080583592012161 1102 34 0.028489113560873136 1102 35 0.21435871242797333 1102 36 0.2840101367355601 1102 37 0.2046879384446001 1102 38 -0.8764318577581349 1102 39 0.06043499624060189 1102 40 0.4137707590416255 1102 41 0.5278767176487449 1102 42 0.19769369779720591 1103 1 1.3361691189657705 1103 2 1.2399580500102019 1103 3 1.3970417792942058 1103 4 1.1735200487836295 1103 5 1.4807116628098882 1103 6 1.2451474855731917 1103 7 1.4090777929661158 1103 8 0.9220323946668714 1103 9 1.334161016625707 1103 10 1.5107313132962261 1103 11 1.1948403529777138 1103 12 1.032013685325559 1103 13 0.09041307430971202 1103 14 0.2634254380727974 1103 15 0.49834868163877033 1103 16 1.1812616816338846 1103 17 0.510722857855832 1103 18 -0.8356245850897288 1103 19 -2.0441714525128205 1103 20 -1.5711029805450067 1103 21 -2.4361370090285526 1103 22 -3.5969527725289616 1103 23 -2.808614678444446 1103 24 -1.5163031988363045 1103 25 -0.8997040016989502 1103 26 -0.8185776071209148 1103 27 -1.7060332164817282 1103 28 -0.2405243905572192 1103 29 0.33386423133754306 1103 30 0.5161149347263461 1103 31 0.5557377674259385 1103 32 0.45190337034125605 1103 33 0.23003687889402613 1103 34 0.2570605421323009 1103 35 0.8048349029041617 1103 36 0.6992482319736553 1103 37 0.9951641289207913 1103 38 0.24166338033710844 1103 39 0.4261492819548849 1103 40 0.5985326638035318 1103 41 0.5374005271725579 1103 42 0.603407983511488 1104 1 0.13616911896576767 1104 2 0.6799580500101996 1104 3 -0.002958220705792769 1104 4 0.5335200487836289 1104 5 0.8007116628098885 1104 6 0.9251474855731914 1104 7 0.6090777929661186 1104 8 0.6020323946668711 1104 9 -0.06583898337429517 1104 10 0.6707313132962227 1104 11 0.43484035297771584 1104 12 -0.12798631467443755 1104 13 -0.02958692569028898 1104 14 -0.09657456192720204 1104 15 0.8583486816387698 1104 16 -1.3787383183661142 1104 17 -0.04927714214417023 1104 18 -1.8356245850897288 1104 19 -2.3241714525128216 1104 20 -0.01110298054500447 1104 21 -0.2761370090285524 1104 22 -2.8769527725289628 1104 23 -2.1686146784444453 1104 24 0.24369680116369352 1104 25 0.5402959983010476 1104 26 -0.05857760712091675 1104 27 -0.3460332164817288 1104 28 -0.8405243905572206 1104 29 0.41386423133754136 1104 30 -0.1238850652736545 1104 31 0.23573776742593822 1104 32 -0.6280966296587422 1104 33 -0.009963121105975858 1104 34 0.537060542132302 1104 35 -0.6751650970958352 1104 36 -0.2607517680263456 1104 37 0.19516412892078705 1104 38 -0.1983366196628964 1104 39 -0.5738507180451151 1104 40 -0.08146733619646795 1104 41 -0.5425994728274475 1104 42 -0.3565920164885128 1105 1 -0.10383088103422722 1105 2 -0.4000419499897987 1105 3 0.7170417792942061 1105 4 0.2935200487836269 1105 5 -0.23928833719011067 1105 6 -0.8348525144268137 1105 7 -0.39092220703388136 1105 8 0.2020323946668725 1105 9 -0.14583898337429702 1105 10 -0.32926868670377374 1105 11 -0.08515964702228729 1105 12 -1.4479863146744378 1105 13 0.25041307430971216 1105 14 -0.2565745619272022 1105 15 1.0983486816387718 1105 16 0.9812616816338817 1105 17 -0.5692771421441734 1105 18 -1.5556245850897277 1105 19 -1.2441714525128198 1105 20 -0.651102980545005 1105 21 0.04386299097144786 1105 22 1.0830472274710345 1105 23 0.5913853215555562 1105 24 0.0036968011636986375 1105 25 -0.1797040016989513 1105 26 0.4614223928790864 1105 27 -0.8260332164817328 1105 28 -0.5605243905572195 1105 29 -0.42613576866245495 1105 30 -0.763885065273648 1105 31 -0.044262232574055815 1105 32 -0.10809662965874622 1105 33 -0.7299631211059747 1105 34 -1.6229394578676946 1105 35 0.24483490290416654 1105 36 -0.1807517680263473 1105 37 0.1551641289207879 1105 38 0.32166338033710673 1105 39 0.30614928195488744 1105 40 0.35853266380352977 1105 41 0.3374005271725551 1105 42 0.6434079835114872 1106 1 -1.3438308810342292 1106 2 -1.4148567648046182 1106 3 -0.15851377626135132 1106 4 -0.8887021734385954 1106 5 -1.590399448301227 1106 6 0.30440674483244834 1106 7 -0.04129257740425629 1106 8 -0.014263901629426812 1106 9 -0.5325056500409637 1106 10 -0.8670464644815539 1106 11 -0.8747892766519136 1106 12 -1.20946779615592 1106 13 -0.6281054442088063 1106 14 -0.29213011748275974 1106 15 0.16353386682395765 1106 16 -0.286886466514261 1106 17 -1.6789067717737964 1106 18 -2.260809770274914 1106 19 -0.9300973784387487 1106 20 -1.1399918694338957 1106 21 1.7105296576381122 1106 22 1.9023064867302963 1106 23 -0.5641702340000023 1106 24 -0.2837106062437087 1106 25 -1.9367410387359882 1106 26 -0.7489479774912908 1106 27 -2.774181364629875 1106 28 -2.302746612779444 1106 29 -0.2690987316254194 1106 30 -0.900181361569949 1106 31 -0.5168548251666536 1106 32 -1.1199484815105976 1106 33 -0.5551483062911586 1106 34 -0.7651616800899177 1106 35 -0.9284984304291726 1106 36 -0.5096406569152307 1106 37 -0.08631735256069106 1106 38 -0.19389217521845126 1106 39 -0.2834803476747396 1106 40 -0.45924511397424794 1106 41 -0.18111799134596396 1106 42 -0.5165920164885094 1107 1 0.8783913411879922 1107 2 1.0295876796398247 1107 3 0.17481955707198438 1107 4 0.7409274561910308 1107 5 1.1133042554024826 1107 6 0.4155178559435626 1107 7 0.9957444596327818 1107 8 0.6153657280002065 1107 9 0.2823091647738494 1107 10 0.9107313132962247 1107 11 0.4955810937184566 1107 12 0.49423590754778246 1107 13 0.5200427039393425 1107 14 0.559721734369095 1107 15 0.08945979274988147 1107 16 0.5649653853375867 1107 17 0.6914635985965703 1107 18 -0.2978468073119487 1107 19 0.9587915104501405 1107 20 -0.25110298054500646 1107 21 0.7846037317121883 1107 22 0.4578620422858535 1107 23 0.8432371734074096 1107 24 0.08665976412665799 1107 25 -0.0848891868841335 1107 26 -0.34154057008387895 1107 27 -0.4778850683335847 1107 28 -0.3768206868535202 1107 29 -0.3061357686624575 1107 30 -0.08536654675513233 1107 31 -0.5538918622036846 1107 32 -0.9347632963254071 1107 33 0.2596665085236509 1107 34 -0.394791309719551 1107 35 -1.187757689688432 1107 36 -0.47260361987819977 1107 37 0.3951641289207899 1107 38 0.39870041737414397 1107 39 0.2720752078808104 1107 40 -0.3481340028631337 1107 41 -0.5514883617163306 1107 42 0.03896353906704775 1108 1 1.211724674521328 1108 2 1.0666247166768628 1108 3 1.06370844596087 1108 4 0.7779644932280689 1108 5 0.9651561072543302 1108 6 1.0081104485361507 1108 7 0.9957444596327818 1108 8 0.6153657280002065 1108 9 1.0230499055145899 1108 10 1.2070276095925223 1108 11 0.9400255381628995 1108 12 0.9016433149551872 1108 13 0.0015241854208234429 1108 14 -0.3291671545197943 1108 15 -0.20683650354641614 1108 16 -1.2868864665142645 1108 17 0.13590804304101312 1108 18 -1.3719208813860249 1108 19 -1.3745418228831916 1108 20 0.45260072315869593 1108 21 -1.0672481201396629 1108 22 -0.9125083280845203 1108 23 -2.7123183821481476 1108 24 -0.8392661617992658 1108 25 -0.6404447424396906 1108 26 0.2140149854716782 1108 27 0.18878159833308672 1108 28 0.5491052390724036 1108 29 0.6938642313375425 1108 30 0.5442630828744939 1108 31 -0.8131511214629441 1108 32 0.3985700370079215 1108 33 -0.11070386184671577 1108 34 0.7903938754656394 1108 35 0.6640941621634227 1108 36 0.6014704541958764 1108 37 0.432201165957828 1108 38 -0.11981810114437508 1108 39 0.2720752078808104 1108 40 -0.12591178064091224 1108 41 0.6336968234688527 1108 42 -0.5906660905625856 1109 1 0.8285829120692156 1109 2 0.813751153458476 1109 3 0.6384210896390314 1109 4 0.8852441867146581 1109 5 1.313815111085752 1109 6 1.0106647269524984 1109 7 1.3290777929661175 1109 8 0.41996342914963236 1109 9 0.7893334304188073 1109 10 0.6348692443307087 1109 11 0.4444955253915097 1109 12 0.8582205818772835 1109 13 -0.6613110636213229 1109 14 -0.0814021481340994 1109 15 0.8059348885353224 1109 16 0.8612616816338843 1109 17 0.9507228578558298 1109 18 0.7864443804275112 1109 19 0.6510009612802818 1109 20 1.2661383987653352 1109 21 1.7680009220059318 1109 22 1.470633434367592 1109 23 1.9569025629348644 1109 24 1.020248525301625 1109 25 1.479606343128637 1109 26 1.5179741170170118 1109 27 0.6153460938630957 1109 28 0.2643031956496742 1109 29 0.7628297485789233 1109 30 0.706459762312555 1109 31 0.31711707777076725 1109 32 0.45604130137574117 1109 33 0.3541748099285087 1109 34 0.04326743868402616 1109 35 -0.07792371778549523 1109 36 0.24131719749089342 1109 37 0.3951641289207899 1109 38 0.5340771734405507 1109 39 0.7944251440238546 1109 40 0.7323257672518082 1109 41 0.44084880303462626 1109 42 0.1730631559252842 1110 1 0.9895024522991065 1110 2 0.366624716676867 1110 3 0.5970417792942087 1110 4 0.7335200487836246 1110 5 0.3207116628098845 1110 6 0.7451474855731917 1110 7 0.2624111262994475 1110 8 0.21536572800020082 1110 9 0.06749434995903769 1110 10 -0.02260202003710532 1110 11 0.2548403529777161 1110 12 0.272013685325561 1110 13 0.45707974097637916 1110 14 0.6300921047394645 1110 15 0.4116820149721043 1110 16 0.06126168163388712 1110 17 0.9507228578558298 1110 18 1.1243754149102756 1110 19 0.8958285474871772 1110 20 1.715563686121662 1110 21 2.8105296576381136 1110 22 3.209713894137707 1110 23 2.5247186548888862 1110 24 1.757030134497029 1110 25 1.4336293316343856 1110 26 1.5214223928790815 1110 27 1.87396678351827 1110 28 1.7194756094427746 1110 29 0.6271975646708725 1110 30 1.562781601393013 1110 31 1.4424044340926088 1110 32 0.86523670367459 1110 33 0.863370212227359 1110 34 0.5570605421323052 1110 35 0.3048349029041617 1110 36 0.6125815653069893 1110 37 0.36183079558745845 1110 38 -0.3050032863295584 1110 39 -0.5871840513784434 1110 40 -0.3814673361964722 1110 41 -0.6292661394941135 1110 42 -0.2832586831551751 1111 1 -0.22261875982210455 1111 2 -0.05458740453525479 1111 3 -1.3302309479785208 1111 4 0.12139883666241502 1111 5 -1.539894397796175 1111 6 -0.40939796897226444 1111 7 -1.0345585706702494 1111 8 -0.3240282113937383 1111 9 -0.3506874682227803 1111 10 -0.2104808079158964 1111 11 -0.27546267732531504 1111 12 -0.33404692073504805 1111 13 0.19344337734001726 1111 14 -0.009301834654475982 1111 15 0.09956080285089186 1111 16 0.7400495604217596 1111 17 0.5264804336134077 1111 18 1.378920869455726 1111 19 -1.7920502403916103 1111 20 0.5367758073337825 1111 21 -0.01674306963461447 1111 22 2.0369866214104277 1111 23 1.233809563979797 1111 24 0.12369680116369608 1111 25 1.4033263013313544 1111 26 0.6820284534851453 1111 27 1.343663753215239 1111 28 0.07099076095792611 1111 29 0.4514399889133003 1111 30 0.30520584381725513 1111 31 0.560586252274426 1111 32 0.4591760976139838 1111 33 0.3270065758637202 1111 34 0.6085756936474525 1111 35 0.2836227816920385 1111 36 0.3489452016706238 1111 37 0.21334594710261 1111 38 -1.0625790439053162 1111 39 -0.44172950592390237 1111 40 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9 -0.7868916149532446 2212 10 -0.0892686867037753 2212 11 -0.9556859628117564 2212 12 -1.0700915778323363 2212 13 -0.32011324147975984 2212 14 -0.4997324566640451 2212 15 -0.5216513183612292 2212 16 -0.0861067394187458 2212 17 0.5033544368031997 2212 18 -0.15457195351077857 2212 19 0.048460126434548556 2212 20 -0.2247871910713215 2212 21 -0.3508738511338123 2212 22 -0.43590014095001806 2212 23 -1.0875620468655 2212 24 -0.6920926725205163 2212 25 -1.0575987385410528 2212 26 -1.1154197123840746 2212 27 0.37396678351827006 2212 28 -0.18578754845196244 2212 29 -0.46403050550456015 2212 30 -0.07756927579996642 2212 31 -1.3716306536266885 2212 32 -0.5049387349219003 2212 33 -0.5278578579480779 2212 34 -0.674518405236114 2212 35 -0.9372703602537342 2212 36 -1.173383346973715 2212 37 -1.3416779763423676 2212 38 -0.2699155670313118 2212 39 -0.18016650751879837 2212 40 -0.339362073038572 2212 41 0.10055842190939757 2212 42 -0.41132885859376955 2213 1 -0.5938308810342292 2213 2 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0.3604747989126835 2214 33 -0.34139169253454327 2214 34 0.14277482784658702 2214 35 -0.3380222399529771 2214 36 -0.04932319659777562 2214 37 -0.2905501567934934 2214 38 -0.8097651910914649 2214 39 -0.6538507180451134 2214 40 -0.08146733619646795 2214 41 -1.662599472827445 2214 42 -0.7737348736313692 2215 1 0.4847405475372 2215 2 0.25710090715305256 2215 3 -1.2601010778486526 2215 4 0.04780576306934137 2215 5 0.4349973770955984 2215 6 -0.9834239429982432 2215 7 -0.18520792131959496 2215 8 0.05346096609544304 2215 9 0.04844673091141871 2215 10 -0.0892686867037753 2215 11 0.5548403529777133 2215 12 0.6434422567541347 2215 13 0.09041307430971202 2215 14 0.4634254380727967 2215 15 0.5783486816387722 2215 16 -0.7101668897946851 2215 17 0.17929428642725753 2215 18 0.8100897006245589 2215 19 -1.2613143096556776 2215 20 -0.33681726625929187 2215 21 0.7295772766857311 2215 22 1.7573329417567507 2215 23 0.33424246441269645 2215 24 0.6665539440208406 2215 25 1.5002959983010484 2215 26 1.0214223928790815 2215 27 0.2882524978039811 2215 28 0.03376132372849128 2215 29 0.35100708848040085 2215 30 0.3675435061549166 2215 31 0.3043091959973694 2215 32 0.07476051319839883 2215 33 0.315751164608308 2215 34 0.8284891135608774 2215 35 0.9476920457613076 2215 36 0.8078196605450856 2215 37 0.36659270034935787 2215 38 0.6759490946228226 2215 39 0.6032921390977464 2215 40 -0.19575305048218183 2215 41 -0.005456615684586552 2215 42 0.7119794120829184 2216 1 0.7361691189657691 2216 2 0.03995805001019903 2216 3 -1.042958220705792 2216 4 -0.7064799512163731 2216 5 -0.4392883371901135 2216 6 -0.35485251442680976 2216 7 -0.4309222070338805 2216 8 0.3620323946668691 2216 9 0.13416101662570412 2216 10 -0.44926868670377473 2216 11 0.27484035297771925 2216 12 0.11201368532556444 2216 13 0.49041307430971415 2216 14 0.2634254380727974 2216 15 0.1383486816387709 2216 16 0.301261681633882 2216 17 0.510722857855832 2216 18 2.004375414910271 2216 19 0.7958285474871758 2216 20 1.1888970194549913 2216 21 0.36386299097144814 2216 22 -0.4769527725289606 2216 23 0.3113853215555551 2216 24 0.603696801163693 2216 25 0.7402959983010504 2216 26 0.4614223928790864 2216 27 0.21396678351827347 2216 28 -0.12052439055722175 2216 29 -1.7461357686624552 2216 30 -0.48388506527365394 2216 31 -0.32426223257405695 2216 32 -0.3480966296587411 2216 33 -0.9299631211059776 2216 34 -1.462939457867698 2216 35 -0.6751650970958352 2216 36 -0.14075176802634815 2216 37 -0.24483587107921068 2216 38 -0.7583366196628916 2216 39 0.026149281954886305 2216 40 -0.16146733619646625 2216 41 0.45740052717255253 2216 42 -0.11659201648851081 >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 11.0 27 33.0 28 82.0 29 158.0 30 306.0 31 504.0 32 1004.0 33 1906.0 34 3110.0 35 3850.0 36 3348.0 37 2486.0 38 2430.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.691699604743083 1.86706885791554 96.48949448720616 0.9517370501352195 2 95.64697316413564 3.203661327231121 0.5668816309548576 0.5824838776783856 3 1.0817557728312877 95.99022259205326 0.6864988558352403 2.241522779280216 4 0.6604951112960267 1.6642396505096735 1.055752028292074 96.61951320990224 5 95.8862076138964 2.652381942999792 0.45246515498231743 1.0089452881214895 6 1.4926149365508634 96.25546078635323 1.6382359059704599 0.6136883711254421 7 0.7437457741717377 2.1636240703177823 93.24387579965673 3.8487543558537474 8 2.527563969211567 1.7214478884959434 0.8737258165175785 94.87726232577491 9 22.239558953554898 24.87647578925469 28.43917407811931 24.4447911790711 10 33.40441023507385 23.07572290409819 22.92490118577075 20.59496567505721 11 33.02127216934519 23.27976283351537 21.110937743797784 22.588027253341654 12 29.302543298486505 23.768658657096793 24.725646226660423 22.203151817756282 13 57.16292134831461 21.681231793591344 9.228048272992092 11.927798585101957 14 23.37736634075307 21.676721447888497 41.19513209902226 13.750780112336175 15 18.708066781089094 19.54022988505747 46.9287980444167 14.822905289436731 16 17.386103598918247 22.98210942375702 41.699604743083 17.93218223424173 17 16.475972540045767 25.21843145412939 12.377782400665696 45.927813605159145 18 44.90167516387473 23.176568515242952 14.244095307460203 17.67766101342212 19 24.715244187860822 35.736204296042025 19.857492068445413 19.69105944765174 20 19.68483461618473 28.03203661327231 24.84917828167256 27.433950488870394 21 27.678385687539002 22.836488454337424 24.823174537133347 24.661951320990223 22 27.335136259621386 25.156022467235285 26.1077595173705 21.40108175577283 23 21.863948408570835 25.145620969419596 29.061784897025174 23.9286457249844 24 22.092781360515914 24.27189515290202 24.381110879966716 29.254212606615354 25 23.62180153942168 23.356563345121696 27.8240066569586 25.197628458498023 26 25.85292282088621 25.30164343665488 26.26378198460578 22.58165175785313 27 23.97025171624714 23.70501352194716 28.552111504056583 23.772623257749114 28 22.59725400457666 28.22966507177033 26.63303515706262 22.54004576659039 29 25.504472644060744 23.72061576867069 28.31287705429582 22.462034532972748 30 29.01497815685459 25.32764718119409 25.338048679009777 20.319325982941542 31 23.347376085712803 29.44817184168097 24.689239090861808 22.515212981744423 32 23.455377574370708 25.353650925733305 28.151653838152697 23.03931766174329 33 24.80757229040982 25.11961722488038 27.70438943207822 22.36842105263158 34 24.80757229040982 26.679841897233203 28.77054295818598 19.742042854171 35 20.927813605159145 27.850010401497816 30.887247763677973 20.334928229665074 36 23.668608279592263 25.31724568337841 27.563969211566462 23.45017682546287 37 25.046806740170585 25.889328063241106 28.77574370709382 20.288121489494486 38 26.019346785937174 26.83586436446848 26.445808196380277 20.698980653214065 39 20.21636240703178 26.98288864617465 29.125708638893222 23.67504030790035 40 22.261520857172577 24.409653594091335 28.05575782794133 25.273067720794756 41 25.87892656542542 26.8618681090077 26.976284584980238 20.282920740586643 42 23.45261624882971 28.378237802975136 27.051908873400603 21.11723707479455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 3.5 9 6.0 10 6.0 11 12.5 12 19.0 13 30.0 14 41.0 15 38.0 16 35.0 17 35.0 18 38.0 19 41.0 20 63.0 21 85.0 22 85.0 23 143.0 24 201.0 25 252.5 26 304.0 27 343.5 28 383.0 29 383.0 30 423.5 31 464.0 32 517.5 33 571.0 34 571.0 35 694.5 36 818.0 37 904.5 38 991.0 39 1096.5 40 1202.0 41 1202.0 42 1268.5 43 1335.0 44 1344.5 45 1354.0 46 1500.5 47 1647.0 48 1647.0 49 1772.5 50 1898.0 51 1876.5 52 1855.0 53 1855.0 54 1646.0 55 1437.0 56 1300.5 57 1164.0 58 1073.0 59 982.0 60 982.0 61 801.0 62 620.0 63 507.5 64 395.0 65 386.5 66 378.0 67 378.0 68 441.5 69 505.0 70 321.0 71 137.0 72 137.0 73 199.0 74 261.0 75 163.5 76 66.0 77 44.5 78 23.0 79 23.0 80 13.5 81 4.0 82 3.0 83 2.0 84 2.0 85 2.5 86 3.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.005200748907842729 8 0.0 9 0.005200748907842729 10 0.0 11 0.005200748907842729 12 0.005200748907842729 13 0.020802995631370915 14 0.0 15 0.005200748907842729 16 0.0 17 0.0 18 0.031204493447056374 19 0.005200748907842729 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.005200748907842729 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.005200748907842729 40 0.010401497815685458 41 0.0 42 0.010401497815685458 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 19228.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.32411067193676 #Duplication Level Percentage of deduplicated Percentage of total 1 76.46352723915051 43.06740170584564 2 12.096029547553092 13.625962138547951 3 4.33056325023084 7.317453713334721 4 2.2899353647276084 5.159142916579987 5 1.348107109879963 3.7965467027251925 6 0.7109879963065558 2.402745995423341 7 0.5724838411819021 2.2571250260037443 8 0.3508771929824561 1.5810276679841897 9 0.24007386888273313 1.2169752444351987 >10 1.4958448753462603 14.889744123153733 >50 0.09233610341643582 3.8537549407114624 >100 0.009233610341643581 0.8321198252548367 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGACGACCTGCCTTTCATGCGCCACCCGCGCGCACGGCCCGG 160 0.8321198252548367 No Hit TGACGATCCCCAGTCGCGGAGGCACGTTACCGCGCACAACCC 96 0.499271895152902 No Hit TGACGACGAAGTCAGCGCTTTGCGCCCCGGCTGCGCACCGTC 92 0.4784688995215311 No Hit TGACGATCCGGGGGGCAGGACCGGTTCGCAGGGACAGTGCAC 87 0.45246515498231743 No Hit TGACGATCCAAAGTAGCCTAGAGCGCCAGGGGCGGAAATTCG 81 0.4212606615352611 No Hit TGACGATCGGGCGTTTCTATTGAAACTGGGTTAAATTGACAT 80 0.41605991262741837 No Hit TGACGATCGCGGGTTTTGACGATCTTCGGACTGTAGAACTCT 77 0.40045766590389015 Illumina DpnII expression Sequencing Primer (95% over 22bp) TGACGATCTCGAGTGGGCAGGTGGGGGAGCACCCTTTCAGAA 60 0.31204493447056375 No Hit TGACGATCTTCGGTTAAAACTTGATGTTTTGATTTAGCTTCG 58 0.3016434366548783 No Hit TGACGATCCCACAATACGACTCACTATAGCTATTGAAGGAAG 56 0.2912419388391928 No Hit TGACGAACTTATAGCGCGTCGACCAGCTGAAGCTTGTTCTTC 54 0.28084044102350736 No Hit TGACGATCTTCTGTTGACTAGGCGCATAGTCGCCATTCACAG 47 0.24443519866860827 No Hit TGACGATCTAAGGTTTAAATTGACATCTTCATAAGGGGTTGG 44 0.2288329519450801 No Hit TGACGATCCTGGGTAAGGAATTGGTGAAGTAAAATGGTGAAG 42 0.21843145412939463 No Hit TGACGATCTGGTGTAAAAGAAGCGGATTTAATTCTGCATTTA 41 0.2132307052215519 No Hit TGACGATCACTCAGTCCGACGATCCGTCTGACTGTAGAACTC 41 0.2132307052215519 No Hit TGACGATCAGCGGTTCACGGGTCAAATGGGGGATTCTTGAAG 40 0.20802995631370919 No Hit TGACGATCGGAAGTAGCCTAGAGCGCCAGGGGCGGAAATTCG 40 0.20802995631370919 No Hit TGACGATGGGATGACCGTCCGGCGCCGGCAACCGATGCCGTG 38 0.1976284584980237 No Hit TGACGACGAAGTGCAGCGCTTTGCGCCCCGGCTGCGCACCGT 36 0.18722696068233824 No Hit TGACAATCCAAGGGTTATCGGTTGGAGGCAGTGCATTGCCAT 35 0.18202621177449552 No Hit TGACGATCATGAGTTGACTAGGCGCATAGTCGCCATTCACAG 35 0.18202621177449552 No Hit TGACGATCCGAAGTTTAAAGAGGCATCTATCACATAAGGCAT 33 0.17162471395881007 No Hit TGACGATCAGATGTCCGACGATCGTGTTGACGATCGTCGGAC 33 0.17162471395881007 No Hit AAACGATCCCAAGCCGATTGCATGGGGCCTGGTAGTCGTCGC 32 0.16642396505096732 No Hit TGACGATCTCGAGGTGGGCAGGTGGGGGAGCACCCTTTCAGA 31 0.1612232161431246 No Hit TGACGATCTTTAGTTTTAATAGCGTTTAAATTGACATCTTCA 31 0.1612232161431246 No Hit TGACGATCAGTCGCTACAGTCCGACGATCGGACTGTAGAACT 30 0.15602246723528188 No Hit TGACAATCGTTGAATACGACTCACTATAGGGAGTCGTATTAC 30 0.15602246723528188 No Hit TGACGATCTTTAGTAAAAGAAGCGGATTTAATTCTGCATTTA 30 0.15602246723528188 No Hit TGACGATCCCGTGCTAAGACAGCTAAGAAAGCTCCAATTGGT 28 0.1456209694195964 No Hit TGACGATCAGAAGTATAGCTTCCCATCTTCTTTGAGAGTTGT 28 0.1456209694195964 No Hit TGACAATCGAGTGTTTTGGATGTTTGGTGCCCTGAGTAGTTT 27 0.14042022051175368 No Hit TGACGATCTCGGGTAAAAGAAGCGGATTTAATTCTGCATTTA 27 0.14042022051175368 No Hit TGACGATCTCCCGGAGCCAGGGATCCCTGGAAAAATGGCTGA 26 0.13521947160391096 No Hit TGCTGATCTAGTGATGTTGGGTTAAGTCCCGCAACGAGCGCA 26 0.13521947160391096 No Hit TGACGGTCCGAGGTCCGCGACCGCATCTAGCTTCAGTGCGGC 25 0.13001872269606823 No Hit TGACGATCACAAATTTCGCAGATGATATCCCGATGCTGACGG 25 0.13001872269606823 No Hit TGACGATCAAGTGTTCAGGATCTTCCGACGACATTGATTGCT 24 0.1248179737882255 No Hit TGACGATCTGACGCGACGATCTGATTGACGGACTGTAGAACT 23 0.11961722488038277 No Hit TGACGATCATGCGCTTAGATATTTTAAAGAGGCATCTATCAC 23 0.11961722488038277 No Hit TGACGATCGGCGGTACCGACCGCCGGAAGGGATCACATTATG 23 0.11961722488038277 No Hit TGACGATCCGTCGTTTGGTGCCCTGAGTAGTTTCCGGGGCGT 22 0.11441647597254005 No Hit TGACGATCTTTCGATGCGGGACATGAAAAAACAGTGCTTCGG 22 0.11441647597254005 No Hit TGACGATCATGAGTTTCTGAGTTGCCTAGAGTATTGACAATT 22 0.11441647597254005 No Hit TGACGATCAACTGTTGACTAGGCGCATAGTCGCCATTCACAG 22 0.11441647597254005 No Hit TGACGATCCGGAGTTTCAGTCTCGACGGGCTAGCACACATCT 21 0.10921572706469732 No Hit TGACGATCCATCGGTCCGACGATCCCGATGACGATCTTTAGA 21 0.10921572706469732 No Hit TGACGATCCAGTGCCAAGGGAATAAGGAACGTTCCATATCGC 21 0.10921572706469732 No Hit TGACGATCAGTAAATTAGCTGGGCGTGGTTGTGCGTGCCTGT 21 0.10921572706469732 No Hit TGACGATGGGATACCGTCCGGCGCCGGCAACCGATGCCGTGC 21 0.10921572706469732 No Hit TGACGATCCGGCGGATCGATTCCAGATGAGGATCCACAAGCG 21 0.10921572706469732 No Hit TGACGATCGAGAGAAGCGGATTTAATTCTGCATTTAATTGAT 21 0.10921572706469732 No Hit TGACGATCAGCCGATTAGCAAGTCTAGCTAATCTAGCTCTAC 21 0.10921572706469732 No Hit TGACGATCTAAGAGTCCGACGATCGGCCTGACGATCTTCGGA 21 0.10921572706469732 No Hit TGACGATCCATCGTCTCGACGGGCTAGCACACATCTGGTTGA 20 0.10401497815685459 No Hit TGACGATCTCTAATTTCACTAATTCCAATACTTATTATTATT 20 0.10401497815685459 No Hit TGACCTTCATGCGGAGCAAATATCGCTGCCTTCGAAAACGTA 20 0.10401497815685459 No Hit TGACGAGAAGCGCGCGCGAAGCGGCTGCTGCTCGCGAAGAAA 20 0.10401497815685459 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.00520074890784273 0.0 0.0 14 0.0 0.0 0.02080299563137092 0.0 0.0 15 0.0 0.0 0.031204493447056378 0.0 0.0 16 0.0 0.0 0.031204493447056378 0.0 0.0 17 0.0 0.0 0.03640524235489911 0.0 0.0 18 0.0 0.0 0.17162471395881007 0.0 0.0 19 0.0 0.0 0.20802995631370919 0.0 0.0 20 0.0 0.0 0.22363220303723735 0.0 0.0 21 0.0 0.0 0.5252756396921157 0.0 0.0 22 0.0 0.0 0.7749115872685667 0.0 0.0 23 0.0 0.0 0.8529228208862076 0.0 0.0 24 0.0 0.0 0.8789265654254212 0.0 0.0 25 0.0 0.0 0.941335552319534 0.0 0.0 26 0.0 0.0 1.4770126898273352 0.0 0.0 27 0.0 0.0 1.575826919076347 0.0 0.0 28 0.0 0.0 1.7006448928645725 0.0 0.0 29 0.0 0.0 1.8358643644684836 0.0 0.0 30 0.0 0.0 2.7771999167880175 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTAGTG 25 1.3797356E-4 36.07572 7 GAGTCCG 35 6.408027E-7 36.07572 12 TCAGCTG 25 1.3797356E-4 36.07572 7 TCCCCAG 25 1.3797356E-4 36.07572 7 GCGACGA 30 9.388741E-6 36.075718 13 CCTGCCT 20 0.0020348043 36.075718 7 TTTCATG 20 0.0020348043 36.075718 13 GTTTTGA 20 0.0020348043 36.075718 13 TCCGGGG 20 0.0020348043 36.075718 7 GGCTTAG 20 0.0020348043 36.075718 12 TCCAAAG 20 0.0020348043 36.075718 7 TCTTTGG 20 0.0020348043 36.075718 7 TGGCTTA 20 0.0020348043 36.075718 11 GATCGAG 35 6.5414315E-7 35.981773 5 GATCTTA 25 1.4012025E-4 35.981773 5 GATCCGA 25 1.4012025E-4 35.981773 5 GATCCCT 25 1.4012025E-4 35.981773 5 GATCCCC 50 2.1282176E-10 35.981773 5 GATCTCG 50 2.1282176E-10 35.981773 5 GATCCAG 25 1.4012025E-4 35.981773 5 >>END_MODULE