FastQCFastQC Report
Thu 26 May 2016
SRR2099220_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099220_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2830234
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTAGCGACGGCAATGCTATCTTCTTACAAATGACCCAGGA49550.17507386315053808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG692550.036.9179953
TAGCGCG43300.036.8992085
CTAGCGC171300.036.7972644
TCTAGCC641900.036.791543
CTAGCGA160100.036.7466964
CTAGCGT201200.036.729784
TCTAGCA607500.036.5925983
TAGCGTA39200.036.5551955
TAGCGTC59700.036.5002865
TAGCGTT57250.036.445395
CTAGCAC161400.036.4392474
CTAGCGG169400.036.4122354
TAGCGAA26200.036.3915145
TAGCCGC37950.036.3444985
TAGCGCA38950.036.3145035
TAGCCGT38350.036.303345
TAGCCCG35750.036.3024675
TAGCGGC40300.036.2924465
TAGCGGG47150.036.2814335
TAGCGCT48950.036.2710465
Produced by FastQC (version 0.11.5)