FastQCFastQC Report
Thu 26 May 2016
SRR2099219_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099219_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85443
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTAGCCTAGGGTTTCAGCCTGAGGAGCTGGTGGAGTACAA1580.18491860070456329No Hit
GATCTAGCAGCTCGTTTGGTGGGACCCAAAAGGCAATGTGAGA1530.17906674625188723No Hit
GATCTAGCCCCCTGTTTAGGTGTTTACACCAAGTAATGCTGAA1400.16385192467492948No Hit
GATCTAGCCCGACAGTCCGACGATCCTGGGGATCTAGCTTTTT990.11586671816298584No Hit
GATCTAGCGACGGCAATGCTATCTTCTTACAAATGACCCAGGA980.11469634727245065No Hit
GATCTAGCTCGTCGATTCGACAACGAAAGTCATATCGAAGAAA980.11469634727245065No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACTC251.2203179E-437.01933335
GCCCCGG251.224537E-436.997667
AGCGTTC452.561137E-936.997666
AGCGTCA452.561137E-936.997666
AGCCGTC501.7462298E-1036.997666
AGCGTAC251.224537E-436.997666
TAATACG251.224537E-436.9976613
AGCCGGT251.224537E-436.997666
GTATTTC251.224537E-436.9976612
AGCCCAC251.224537E-436.997666
TAGCGGC1050.036.997665
TAGCGCT1050.036.997665
TAGCCGC1050.036.997665
TAGCCCG1050.036.997665
TTTCACT1000.036.9976615
TAGTTCT251.224537E-436.9976613
AGCGGTG452.561137E-936.997666
AGCGGTC501.7462298E-1036.997666
TAGCACG1000.036.997665
AGCGGCG501.7462298E-1036.997666
Produced by FastQC (version 0.11.5)