FastQCFastQC Report
Thu 26 May 2016
SRR2099218_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099218_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2461762
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACGTATAAAAGAGTCGTATTACCGGCCGACGATCTAAAAT58150.2362129239138471No Hit
TGGACGTCCGAGCCAGCACCGACTGCCATTCTCCCTCGCGGCT38700.1572044738687168No Hit
TGCACGTGTATGAGGCACCCGCATCGCCACCAGCTACCCGGCC32330.13132869871254818No Hit
TGCACGTAAGGTTGGGTAGTTCAGCGGTTTAGCGCTGCCTTCA25560.10382807111329202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAC507250.036.9145363
CGTACGG36950.036.8296135
CGTACGC24600.036.8042565
CACGTAA488800.036.7930033
CACGTAG564050.036.7483373
ACGTACG120200.036.718994
ACGTAGC155100.036.704754
ACGTAAC134500.036.682924
ACGTATC173750.036.6541024
ACGTACA123150.036.6512034
ACGTATA144350.036.5781444
CGTAGGG40450.036.5723275
ACGTAAT130250.036.5721934
ACGTATG152250.036.5284774
ACGTACT135200.036.5066874
CGTAGCG40950.036.4874845
ACGTACC134750.036.477474
CGTAAGG38950.036.4596255
CGTAAGC31900.036.4496425
CGTAGAC25300.036.444595
Produced by FastQC (version 0.11.5)