FastQCFastQC Report
Thu 26 May 2016
SRR2099216_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099216_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024120
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGATCGAGCACCGACGATCTAGGTGCTGATCGTCGGACTGT95630.9337772917236262No Hit
CTAGATCGTTCGCACGACTCACTATAGGGAGTCGTATTACCGG48220.4708432605554037No Hit
CTAGATCGCCAATGACATCGCTGACGGAGATGTCGTAGTGCTC40230.3928250595633324No Hit
CTAGATCGCGACTAGTGAGTCGTATTACCGGCCGACGATCTAG30470.29752372768816154No Hit
CTAGATCGCGACTGAACCCCTATAGTGAGTCGTATTACCGGCC30040.29332500097644804No Hit
CTATATTGAGTCGGCGCGAATGGGCGTGGCTGGCGCTACGCAC28500.2782877006600789No Hit
CCAGTTCGAGCCGGTCGAAGATCAGGGCCGAGTTGGCGGGAAT28310.276432449322345No Hit
CTAGATCGGAGTGGAACCCCTATAGTGAGTCGTATTACCGGCC27000.2636409795727063No Hit
CTAGATCGCGACTGAGTCGTATTACCGGCCGACGATCTAGAGT26810.2617857282349725No Hit
CTAGATCGCGACTGGCCGACGATCTAGAGTCGGATCGTCGGAC24820.24235441159239152No Hit
CTAGATCGCGACTACGATCTAGAGTCGGATCGTCGGACTGTAG23210.2266335976252783No Hit
CTAGATCGGAGTGGAGTCGTATTACCGGCCGACGATCTAGCAC20100.1962660625707925No Hit
CTAGATCGGAATCGACTCACTATAGGGAGTCGTATTACCGGCC20100.1962660625707925No Hit
CTAGATCGGAATCGAGTCGTATTACCGGCCGACGATCTAGGAT19500.19040737413584347No Hit
CTAGATCGGAGTGAGTGAGTCGTATTACCGGCCGACGATCTAG18560.1812287622544233No Hit
CTAGATCGTTATGGAACCCCTATAGTGAGTCGTATTACCGGCC18520.1808381830254267No Hit
CTAGATCGCGACTGCCGACGATCTAGAGTCGGATCGTCGGACT18480.1804476037964301No Hit
CTAGATCGCGACTAGTCGGATCGTCGGACTGTAGAACTCTGAA17740.17322188805999297Illumina DpnII expression Sequencing Primer (100% over 25bp)
CTAGATCGCGACTGACGATCTAGAGTCGGATCGTCGGACTGTA16870.16472678982931688No Hit
CTGGATCGGTTTGGGCTCCACGATGCCGCGGCGCAGCAGCCAC15050.14695543490997148No Hit
CTAGATCGCGACTAGAACTCTGAACCCCTATAGTGAGTCGTAT14860.1451001835722376No Hit
CTAGATGTCAGTTGCAGCCAGGTCACGGTCCTGGCGCGCAGCA14600.14256141858375973No Hit
CTAGATCGAGCACGAACCCCTATAGTGAGTCGTATTACCGGCC14490.14148732570401906No Hit
CTAGATCGCGACTACCGGCCGACGATCTAGAGTCGGATCGTCG14120.13787446783580048No Hit
CTAGATCGCGACTACTCTGAACCCCTATAGTGAGTCGTATTAC14040.13709330937780728No Hit
CCAGTTCGAGCCGGGTCGAAGATCAGGGCCGAGTTGGCGGGAA13970.13640979572706324No Hit
CTAGATCGGAATCGAACCCCTATAGTGAGTCGTATTACCGGCC13080.12771940788188885No Hit
CTAGATCGGAGTGACGACTCACTATAGTGAGTCGTATTACCGG12890.12586415654415498No Hit
CTAGATCGGAGTGGGCCGACGATCTAGCACTCGATCGTCGGAC12720.12420419482091942No Hit
CTAGATCGCGACTAGTCGTATTACCGGCCGACGATCTAGAGTC12690.12391126039917198No Hit
CTAGATCGAGCACGGTGCTGATCGTCGGACTGTAGAACTCTGA12690.12391126039917198Illumina DpnII expression Sequencing Primer (100% over 24bp)
CTAGATCGGAGTGACCGGCCGACGATCTAGCACTCGATCGTCG12630.12332539155567707No Hit
CTAGATCGTTCGCGAACCCCTATAGTGAGTCGTATTACCGGCC12500.12205600906143811No Hit
CTAGATCGGAGTGCGGCCGACGATCTAGCACTCGATCGTCGGA12000.1171737686989806No Hit
CTAGATCGGAGTGGTAGAACTCTGAACCCCTATAGTGAGTCGT11800.11522087255399759No Hit
CTAGATCGCGACTCGGCCGACGATCTAGAGTCGGATCGTCGGA11750.11473264851775183No Hit
CTAGATCGGAGTGAGCACTCGATCGTCGGACTGTAGAACTCTG11740.11463500371050267Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTAGATCGGAATCAGTGAGTCGTATTACCGGCCGACGATCTAG11690.11414677967425692No Hit
CTAGATCGGAGTGCCGGCCGACGATCTAGCACTCGATCGTCGG11640.11365855563801118No Hit
CTAGATCGGAGTGACGATCTAGCACTCGATCGTCGGACTGTAG11550.11277975237276883No Hit
CTAGATCGGAGTGGCCGACGATCTAGCACTCGATCGTCGGACT11460.11190094910752647No Hit
CTAGATCGAGCACAGGTGCTGATCGTCGGACTGTAGAACTCTG11410.11141272507128072Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTAGATCGCGACTGTAGAACTCTGAACCCCTATAGTGAGTCGT11380.11111979064953326No Hit
CTAGATCGAGCACGCGACGATCTAGGTGCTGATCGTCGGACTG10490.10242940280435887No Hit
CTAGATCGAGCACGCTGATCGTCGGACTGTAGAACTCTGAACC10440.10194117876811311Illumina DpnII expression Sequencing Primer (100% over 27bp)
CTAGATCGGAGTGGAACTCTGAACCCCTATAGTGAGTCGTATT10250.10008592743037925No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG200.001833375837.034737
GACACGC200.001833375837.034738
CGTACGT950.037.034737
GGTACGC355.5892633E-737.0347258
TGGCGAA200.001842641936.99672710
TACACCG551.2732926E-1136.9696272
AGATCGG256050.036.9371073
CGCGACT56350.036.870427
AGATCGC291550.036.8587763
CGTTATG24950.036.8120737
ATCGCGA61350.036.787875
CGGAGTG47950.036.6871647
CGTTCGC26600.036.6866577
GCGACTA19750.036.659698
ATCGGGG18050.036.6195
ATCGGAG56000.036.5994875
GATCGCT61200.036.5701374
AGATCGA167100.036.5696533
ATCGCAT15350.036.546835
GATCGGA108100.036.544374
Produced by FastQC (version 0.11.5)