FastQCFastQC Report
Thu 26 May 2016
SRR2099215_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099215_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24794
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGATCGGAGTTCGTCCGACGATCACACTCTAGATCGTTTTT2661.0728402032749857No Hit
CTAGATCGACCTCGGTACAGTGCTCTACGCCGGAAGTTCTACC2050.8268129386141808No Hit
CTAGATCGAGCACCGACGATCTAGGTGCTGATCGTCGGACTGT1830.7380817939824151No Hit
CTAGATCGTTCGCACGACTCACTATAGGGAGTCGTATTACCGG1370.5525530370250867No Hit
CTAGATCGCGACTGAACCCCTATAGTGAGTCGTATTACCGGCC760.3065257723642817No Hit
CTAGATCGCCAATGACATCGCTGACGGAGATGTCGTAGTGCTC740.2984593046704848No Hit
CTAGATCGTTATGGAACCCCTATAGTGAGTCGTATTACCGGCC690.27829313543599254No Hit
CTAGATCGCGACTAGTGAGTCGTATTACCGGCCGACGATCTAG690.27829313543599254No Hit
CTAGATCGGAGTGGAACCCCTATAGTGAGTCGTATTACCGGCC580.2339275631201097No Hit
CTAGATCGCGACTGAGTCGTATTACCGGCCGACGATCTAGAGT580.2339275631201097No Hit
CCAGTTCGAGCCGGTCGAAGATCAGGGCCGAGTTGGCGGGAAT580.2339275631201097No Hit
CTAGATCGCGACTACGATCTAGAGTCGGATCGTCGGACTGTAG530.21376139388561746No Hit
CTATATTGAGTCGGCGCGAATGGGCGTGGCTGGCGCTACGCAC510.20569492619182062No Hit
CTAGATCGCTGATGGTCCGACGATCTTTTTCTAGATCGTCGGA470.1895619908042268No Hit
CTAGATCGGAGTGAGTGAGTCGTATTACCGGCCGACGATCTAG460.1855287569573284No Hit
CTAGATCGCGACTGGCCGACGATCTAGAGTCGGATCGTCGGAC430.17342905541663306No Hit
CTAGATCGTTATGACCGGCCGACGATCTAGCATAAGATCGTCG430.17342905541663306No Hit
CTAGATCGTTATGCGGCCGACGATCTAGCATAAGATCGTCGGA420.16939582156973462No Hit
CTAGATCGGAATCGACTCACTATAGGGAGTCGTATTACCGGCC420.16939582156973462No Hit
CTAGATCGCGGGCAAGTCGACCACAATAAAGAGAAAACGAGAT420.16939582156973462No Hit
CTAGATCGGAATCGAGTCGTATTACCGGCCGACGATCTAGGAT410.16536258772283619No Hit
CTAGATCGCTTGTAGTCCGACGATCTTAGGCTAGATCGTTGGA400.16132935387593772No Hit
CTAGATCGCAAGTGGTTCAAATCCCAGCAACCACATGGTGGCT390.15729612002903928No Hit
CTAGATCGCGACTGACGATCTAGAGTCGGATCGTCGGACTGTA390.15729612002903928No Hit
CTAGATGTCAGTTGCAGCCAGGTCACGGTCCTGGCGCGCAGCA380.15326288618214085No Hit
CTAGATCGTTCGCGAACCCCTATAGTGAGTCGTATTACCGGCC370.1492296523352424No Hit
CTAGATCGTTATGGGCCGACGATCTAGCATAAGATCGTCGGAC370.1492296523352424No Hit
CTAGATCGGAGTGGAGTCGTATTACCGGCCGACGATCTAGCAC370.1492296523352424No Hit
CTAGATCGCGACTGCCGACGATCTAGAGTCGGATCGTCGGACT360.14519641848834394No Hit
CTAGATCGTTATGGAGTCGTATTACCGGCCGACGATCTAGCAT360.14519641848834394No Hit
CTAGATCGCGACTAGAACTCTGAACCCCTATAGTGAGTCGTAT350.1411631846414455No Hit
CTAGATCGGAATCGAACCCCTATAGTGAGTCGTATTACCGGCC340.13712995079454707No Hit
CTAGATCGCTCAAGGTTCAGGAAGTTTTTAGCTCTGTACTTGC330.13309671694764863No Hit
CTAGATCGCGACTAGTCGGATCGTCGGACTGTAGAACTCTGAA320.1290634831007502Illumina DpnII expression Sequencing Primer (100% over 25bp)
CTAGATCGGAGTGCCGGCCGACGATCTAGCACTCGATCGTCGG320.1290634831007502No Hit
CCAGTTCGAGCCGGGTCGAAGATCAGGGCCGAGTTGGCGGGAA320.1290634831007502No Hit
CTAGATCGGAGTGACCGGCCGACGATCTAGCACTCGATCGTCG320.1290634831007502No Hit
CTAGATCGAGCACGAACCCCTATAGTGAGTCGTATTACCGGCC310.12503024925385173No Hit
CTAGATCGTTATGGTAGAACTCTGAACCCCTATAGTGAGTCGT310.12503024925385173No Hit
CTAGATCGTTACAAGTCCGACGATCTCAGACTAGATCGTCGGA310.12503024925385173No Hit
CTAGATCGAGGCCGATCCGACGATCGGGTTCTAGAACTCTGAA300.1209970154069533No Hit
CTAGATCGGAGTGACGACTCACTATAGTGAGTCGTATTACCGG300.1209970154069533No Hit
CTAGATCGCGACTAGTCGTATTACCGGCCGACGATCTAGAGTC300.1209970154069533No Hit
CTGGATCGGTTTGGGCTCCACGATGCCGCGGCGCAGCAGCCAC290.11696378156005485No Hit
CTAGATCGGAGTCAGTCCGACGATCGCGCGCTAGATCGTTGGA290.11696378156005485No Hit
CTAGATCGGCTGACAGTCCGACGATCTGGTACTAGATCGTCGG290.11696378156005485No Hit
CTAGATCGGAATCAGTGAGTCGTATTACCGGCCGACGATCTAG290.11696378156005485No Hit
CTAGATCGTTATGATTACCGGCCGACGATCTAGCATAAGATCG280.1129305477131564No Hit
CTAGATCGAACCCGGTTTGAATAAAGAAAGGTATTAATGGGTC280.1129305477131564No Hit
CTAGATCGGAGTGGCCGACGATCTAGCACTCGATCGTCGGACT260.10486408001935951No Hit
CTAGATCGTTATGAGTGAGTCGTATTACCGGCCGACGATCTAG260.10486408001935951No Hit
CTAGATCGTTATGCCGGCCGACGATCTAGCATAAGATCGTCGG250.10083084617246109No Hit
CTAGATCGGAGTGACGATCTAGCACTCGATCGTCGGACTGTAG250.10083084617246109No Hit
CTAGATCGAGCACGAGTCGTATTACCGGCCGACGATCTAGGTG250.10083084617246109No Hit
CTAGATCGGAGTGGGCCGACGATCTAGCACTCGATCGTCGGAC250.10083084617246109No Hit
CTAGATCGCCATGAGTCCGACGATCAAGCTCTAGATCGTCGGA250.10083084617246109No Hit
CTAGATCGTGGGCAGTCCGACGATCGGCATCTAGATCGTCGGA250.10083084617246109No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGC800.037.07
CGCGACT800.037.07
ACAGTGC200.001807362837.017
TTATGCG251.1990762E-437.09
CACACTC403.5806806E-837.025
CGTTATG1000.037.07
CAGTTCG200.001807362837.02
TCGACCT200.001807362837.06
GCGACTG355.340571E-737.08
CCAGTTC200.001807362837.01
GCGACTA307.988003E-637.08
TTCGTCC403.5806806E-837.012
TGGTAGA200.001807362837.012
GGACCGA200.001807362837.03
GAAGTTC200.001807362837.033
CCTCGGT200.001807362837.010
TCGGCCA200.001807362837.06
TCGGTCG200.001807362837.06
GATCGTA1100.037.04
AAATACG200.001807362837.013
Produced by FastQC (version 0.11.5)