##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099215_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24794 Sequences flagged as poor quality 0 Sequence length 43 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.60381543921917 37.0 35.0 37.0 32.0 37.0 2 35.60655803823506 37.0 35.0 37.0 32.0 37.0 3 34.505606195047186 35.0 35.0 37.0 32.0 37.0 4 36.81995644107445 39.0 37.0 39.0 32.0 39.0 5 34.816608856981524 37.0 34.0 39.0 30.0 39.0 6 36.20194401871421 37.0 35.0 39.0 32.0 39.0 7 37.12511091393079 39.0 37.0 39.0 33.0 39.0 8 36.828587561506815 39.0 37.0 39.0 32.0 39.0 9 31.484270387997096 33.0 31.0 34.0 28.0 34.0 10 31.78865854642252 34.0 31.0 34.0 28.0 34.0 11 31.83798499637009 34.0 31.0 34.0 28.0 34.0 12 35.291239816084534 37.0 35.0 37.0 32.0 37.0 13 35.289223199161086 37.0 35.0 37.0 32.0 37.0 14 31.422682907154957 31.0 31.0 34.0 30.0 34.0 15 31.325078648060014 31.0 31.0 34.0 28.0 34.0 16 30.39832217471969 31.0 30.0 34.0 26.0 34.0 17 33.72949100588852 35.0 33.0 37.0 28.0 37.0 18 34.83044284907639 35.0 35.0 37.0 32.0 37.0 19 33.73779946761313 35.0 33.0 37.0 28.0 37.0 20 33.66092603049125 35.0 33.0 37.0 28.0 37.0 21 34.06388642413487 35.0 33.0 37.0 29.0 37.0 22 35.6055900621118 37.0 34.0 39.0 30.0 39.0 23 36.18097120271033 38.0 35.0 39.0 31.0 39.0 24 36.54944744696297 38.0 35.0 39.0 32.0 39.0 25 36.963579898362504 39.0 37.0 39.0 33.0 39.0 26 37.03371783496007 39.0 37.0 39.0 33.0 39.0 27 38.434580947003305 40.0 37.0 41.0 34.0 41.0 28 38.62394127611519 40.0 38.0 41.0 36.0 41.0 29 38.7819230458982 40.0 38.0 41.0 36.0 41.0 30 38.775671533435506 40.0 38.0 41.0 36.0 41.0 31 38.82443333064451 40.0 38.0 41.0 36.0 41.0 32 38.869121561668145 40.0 38.0 41.0 36.0 41.0 33 38.88194724530128 40.0 38.0 41.0 36.0 41.0 34 38.88307655077841 40.0 38.0 41.0 36.0 41.0 35 38.838670646124065 40.0 38.0 40.0 36.0 41.0 36 38.85536823425022 40.0 38.0 41.0 36.0 41.0 37 38.906025651367266 40.0 38.0 41.0 36.0 41.0 38 38.78272969266758 40.0 38.0 41.0 35.0 41.0 39 38.74352665967573 40.0 38.0 41.0 35.0 41.0 40 38.72352181979511 40.0 38.0 41.0 35.0 41.0 41 38.74663224973784 40.0 38.0 41.0 35.0 41.0 42 38.679277244494635 40.0 38.0 41.0 35.0 41.0 43 38.593813019278855 40.0 38.0 41.0 35.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.690330698657533 1101 2 0.7081232810980893 1101 3 0.6665478221443877 1101 4 1.339541770963514 1101 5 1.1576263379930367 1101 6 1.3540493329487617 1101 7 1.1453911221129545 1101 8 0.5299275043105283 1101 9 1.041108141519043 1101 10 0.8576607786444761 1101 11 0.5392965705371182 1101 12 0.3001209516773642 1101 13 0.6101634194984484 1101 14 0.14788677062455236 1101 15 -0.12474005998508275 1101 16 0.44457127989995016 1101 17 -0.13712863461884695 1101 18 0.10422360753128146 1101 19 0.25747524199699257 1101 20 -0.6762298202448846 1101 21 0.38244775514163365 1101 22 -0.6877194456345137 1101 23 1.0409774895308175 1101 24 0.23565114816465638 1101 25 0.3594352702338597 1101 26 0.00806854220507347 1101 27 -0.23281618669057025 1101 28 0.10205914115417158 1101 29 0.1415933879878679 1101 30 0.029445923907012173 1101 31 -0.11075829605594834 1101 32 0.43082514962449636 1101 33 0.24399236007398883 1101 34 0.21952524583325328 1101 35 -0.004901524094712784 1101 36 0.24334510564178657 1101 37 0.3073913720174559 1101 38 0.010138602593080748 1101 39 -0.7385396625572724 1101 40 -0.449491970647685 1101 41 -0.4708099817702305 1101 42 -0.12990890137083966 1101 43 -0.7575392852977032 1102 1 0.9439192154039446 1102 2 0.9577245570151547 1102 3 1.442464887535138 1102 4 0.9735130628295465 1102 5 0.979795397004203 1102 6 1.1427255689934128 1102 7 1.1015314729901462 1102 8 1.376817456463634 1102 9 0.8640746486960786 1102 10 0.7659542395695205 1102 11 0.8901737635195701 1102 12 1.1151129771957002 1102 13 1.1261123828158333 1102 14 0.5498006462226357 1102 15 0.7117830660117299 1102 16 0.9262299720849612 1102 17 1.4410212856363316 1102 18 0.8227244049794464 1102 19 1.3547639182330329 1102 20 0.2145197810310293 1102 21 0.05150676630590567 1102 22 -0.9692186481863487 1102 23 0.7419344273298591 1102 24 0.72129708117901 1102 25 0.5484304855448627 1102 26 0.002486405043988782 1102 27 0.11806100629188165 1102 28 -0.18581964672461737 1102 29 0.3297911551330017 1102 30 0.4544857963153035 1102 31 -0.3659417091340984 1102 32 -0.3434970194345155 1102 33 -0.1906168903247334 1102 34 0.13499574025111372 1102 35 0.28855940094516086 1102 36 0.36535467501977337 1102 37 0.6056369860525379 1102 38 0.3562311065803243 1102 39 0.8731030806644213 1102 40 -0.07229898819154812 1102 41 -0.0409854203667237 1102 42 0.47854404918737004 1102 43 0.5438961214008629 1103 1 0.5940740141655567 1103 2 0.6992106251266108 1103 3 0.6576351661729092 1103 4 1.2165471185571022 1103 5 1.44728765706612 1103 6 1.3950475504175657 1103 7 1.0473519064266767 1103 8 1.2839381994976904 1103 9 0.14271241959390935 1103 10 0.19010284638066466 1103 11 0.5883161783802535 1103 12 0.6940603456167551 1103 13 0.8490225995341021 1103 14 0.014196931052357087 1103 15 0.13283569759067504 1103 16 -0.6418814830234005 1103 17 -0.8020127700912241 1103 18 -0.5116409200979533 1103 19 -0.5972484656678958 1103 20 -0.8241799093714448 1103 21 -2.4268214070687044 1103 22 -4.185936914440216 1103 23 -2.408220371431753 1103 24 -1.996969172690953 1103 25 -0.2983187404613332 1103 26 -0.4402380531603427 1103 27 -0.566149520023906 1103 28 0.049474470922440617 1103 29 -1.386927111120869 1103 30 0.05974895421004334 1103 31 0.332200705726585 1103 32 0.18127078242306993 1103 33 -0.3237438253092577 1103 34 0.18298335635018503 1103 35 0.23128385914949234 1103 36 -0.23526452002666076 1103 37 0.2975874504488232 1103 38 -0.04779366122153306 1103 39 -0.0665254023077182 1103 40 0.12893940190133435 1103 41 -0.010025668044740144 1103 42 0.23550999345981438 1103 43 0.3333698056113903 1104 1 0.07474628307311804 1104 2 0.37652154949635275 1104 3 0.534946090542654 1104 4 0.7291521605739035 1104 5 0.6061111864778894 1104 6 -0.0520112731118445 1104 7 0.35491493163676324 1104 8 -0.8908517164686955 1104 9 0.7242250246359241 1104 10 0.760691081674782 1104 11 0.5488203800609242 1104 12 0.5226317741881843 1104 13 0.04566125499628981 1104 14 0.28814651088429244 1104 15 0.17569284044781952 1104 16 0.3051773405060132 1104 17 -0.11461781210802968 1104 18 0.1934010967087758 1104 19 0.11115489567664127 1104 20 -0.4208185648336311 1104 21 0.3530105257044056 1104 22 -0.05568481359988198 1104 23 0.16825021680354268 1104 24 0.5551316676451776 1104 25 -0.20420109340251003 1104 26 0.5942157283522604 1104 27 0.4624219085475261 1104 28 -0.08581964672461595 1104 29 -0.05667501028052868 1104 30 0.37403466849576006 1104 31 0.43556205026440153 1104 32 0.4602623790617244 1104 33 0.28381919990082594 1104 34 0.26281528912329577 1104 35 0.4825443633511739 1104 36 0.7706178329145104 1104 37 -0.02594196131587978 1104 38 0.4404416328961105 1104 39 0.5670880430704344 1104 40 0.6457461245904099 1104 41 0.4244281134678616 1104 42 0.46576209430015325 1104 43 0.704798377039964 1105 1 -0.4435076851808546 1105 2 -0.40046257748777236 1105 3 0.2246286302251974 1105 4 -0.34227641085466587 1105 5 1.0616667420334451 1105 6 0.05354428244370979 1105 7 -0.2990533223314955 1105 8 0.4996244740074971 1105 9 0.6345424849533821 1105 10 0.3551355261192235 1105 11 0.23374101498156108 1105 12 0.638504790061198 1105 13 0.42581998515501596 1105 14 0.13021000294778418 1105 15 0.35505791981289647 1105 16 0.3107328960615696 1105 17 -0.38207812956834175 1105 18 -1.1962814429737705 1105 19 -1.0531308186090698 1105 20 0.5434671494520842 1105 21 0.20062957332345377 1105 22 0.9006643927493272 1105 23 0.12936132791465837 1105 24 0.021004683518192735 1105 25 -0.19864553784695005 1105 26 -0.2899112557747259 1105 27 0.3227393688649798 1105 28 -0.3247085356135031 1105 29 0.06078530717978481 1105 30 0.19863784309892907 1105 31 0.14429220899455686 1105 32 0.2515322203315691 1105 33 0.38540650148812716 1105 34 0.39376767007567537 1105 35 -0.11348738268056735 1105 36 0.2206178329145132 1105 37 -0.02594196131587978 1105 38 0.5126638551183333 1105 39 0.10994518592757885 1105 40 0.6893969182412008 1105 41 0.08474557378531955 1105 42 0.5998890784271396 1105 43 0.6944809167225046 1106 1 0.5058387200479046 1106 2 0.6992106251266108 1106 3 0.9811645779376121 1106 4 0.18713535385121816 1106 5 -0.346829989992699 1106 6 -0.1931877437000793 1106 7 0.07676367113256077 1106 8 -0.3337088593258386 1106 9 0.14271241959390935 1106 10 0.6312793169688966 1106 11 0.35302206073319553 1106 12 0.047001522087342096 1106 13 0.7019637760046891 1106 14 0.4553734016405926 1106 15 0.33871805053185255 1106 16 -0.906587365376339 1106 17 0.021516641673485992 1106 18 0.13541790343145976 1106 19 0.22628094609681426 1106 20 -1.5888857917243868 1106 21 -1.0738802305981139 1106 22 -0.4212310320872774 1106 23 0.6211913932741311 1106 24 0.29714847436786584 1106 25 0.17226949483278986 1106 26 0.8244678291925993 1106 27 0.02208577409373902 1106 28 0.3730038826871507 1106 29 0.995425830055602 1106 30 0.6185724836218114 1106 31 0.832200705726585 1106 32 0.622447253011309 1106 33 0.029197351161329266 1106 34 0.21239512105606906 1106 35 0.08422503562008643 1106 36 0.2353237152674552 1106 37 0.5622933328017652 1106 38 0.3051475152490539 1106 39 0.22759224475110784 1106 40 0.36423351954839234 1106 41 -0.4512021386329792 1106 42 -0.14684294771664952 1106 43 0.06866392325844828 1107 1 -0.1935076851808546 1107 2 0.3773152002900062 1107 3 -0.3587047031081383 1107 4 -0.12005418863244444 1107 5 -0.743888813522112 1107 6 -1.640900162000733 1107 7 -0.3823866556648241 1107 8 0.19406891845193996 1107 9 -0.05990195949106081 1107 10 0.16069108167478063 1107 11 0.26151879275933965 1107 12 0.055171456727869383 1107 13 -0.40751334817831264 1107 14 0.07465444739222704 1107 15 -0.11716430240932496 1107 16 -0.2726004372717661 1107 17 -0.4098559073461203 1107 18 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-0.06862445928942407 2216 14 0.6595750823128625 2216 15 0.8614071261621028 2216 16 0.9908916262202965 2216 17 1.8853821878919703 2216 18 1.0791153824230548 2216 19 0.9397263242480705 2216 20 1.6934671494520828 2216 21 1.7530105257044042 2216 22 1.8586009006858362 2216 23 1.4825359310892594 2216 24 1.212274524788036 2216 25 1.4243703351689234 2216 26 0.994215728352259 2216 27 0.6909933371189538 2216 28 0.5141803532753855 2216 29 0.314753561148045 2216 30 0.7168918113529017 2216 31 0.34984776455011257 2216 32 0.5745480933474383 2216 33 0.3981049141865398 2216 34 0.8913867176947221 2216 35 0.22540150620832122 2216 36 0.2849035472002299 2216 37 0.6883437529698355 2216 38 0.7832987757532521 2216 39 0.5385166144990094 2216 40 0.7314604103046918 2216 41 0.36728525632500464 2216 42 0.3800478085858714 2216 43 0.6762269484685319 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 1.0 25 0.0 26 4.0 27 25.0 28 67.0 29 128.0 30 287.0 31 425.0 32 701.0 33 1202.0 34 2258.0 35 4045.0 36 5960.0 37 5068.0 38 4623.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.0970396063563765 1.4882632895055254 0.7501814955231104 96.66451560861499 2 1.0526740340404936 1.9238525449705575 95.45051222069856 1.5729612002903928 3 1.3309671694764862 96.68468177784948 1.0688069694280873 0.9155440832459467 4 97.74138904573687 0.4517221908526256 1.1817375171412439 0.6251512462692588 5 0.6775832862789385 97.74542227958378 1.1252722432846656 0.4517221908526256 6 0.6453434437139515 0.48400758278546363 98.09623684104383 0.7744121324567419 7 0.7582479632169072 0.6251512462692588 1.056707267887392 97.55989352262644 8 96.60388012745533 1.351187835276086 1.6093252127616666 0.4356068245069173 9 27.56019844310894 20.235550356955592 21.421368934779977 30.782882265155486 10 27.768815035895784 22.920867951923853 24.95361781076067 24.356699201419698 11 27.636832977049973 26.096075505182913 19.005364417375873 27.26172710039124 12 23.135562457145163 19.38853708708103 29.790666720445287 27.685233735328517 13 24.40106477373558 17.14931031701218 28.599661208356856 29.849963700895376 14 44.429654727331396 30.47353985156502 8.902065182316877 16.194740238786707 15 31.128498830362183 29.22077922077922 23.52181979511172 16.128902153746875 16 20.17667701988625 20.717195756524546 43.043846557218345 16.06228066637086 17 15.628781156731467 23.759780592078727 39.02960393643624 21.58183431475357 18 16.42736145841736 21.283375010083088 13.36613696862144 48.92312656287812 19 39.535258996288526 23.265289656285297 15.378408907535905 21.82104243989027 20 23.467247499193288 32.732333010648595 22.374152952565343 21.426266537592774 21 19.27079132048076 26.373316124868918 24.159070742921674 30.19682181172864 22 26.00629184480116 23.759780592078727 25.53843671856094 24.695490844559167 23 23.5782850689683 26.849237718802936 27.018633540372672 22.553843671856093 24 19.34742276357183 33.58877147697024 28.075340808260062 18.98846495119787 25 20.016939582156972 23.703315318222153 26.744373638783575 29.5353714608373 26 20.323465354521257 22.66274098572235 33.06041784302654 23.953375816729856 27 27.575219811244654 23.34032427200129 25.215778010809064 23.86867790594499 28 25.74413164475276 26.744373638783575 23.88884407517948 23.62265064128418 29 23.34435750584819 27.16382995886101 25.853028958619024 23.638783576671777 30 23.134629345809472 27.413890457368716 27.12753085423893 22.323949342582882 31 23.134629345809472 27.159796725014118 24.626925869161894 25.07864806001452 32 21.327740582398967 28.063241106719367 27.930144389771723 22.678873921109947 33 20.605791723804145 29.57973703315318 29.25707832540131 20.557392917641366 34 25.82479632169073 31.57618778736791 24.02194079212713 18.57707509881423 35 23.687182382834557 33.213680729208676 25.51827054932645 17.58086633863031 36 20.859885456158747 27.66395095587642 32.241671372106154 19.234492215858676 37 23.059858018715715 25.78251048725395 26.669893514036787 24.487737979993547 38 23.75574735823183 27.962410260546903 26.853270952649833 21.428571428571427 39 23.328224570460595 28.29313543599258 27.192062595789302 21.18657739775752 40 23.020450970110122 25.440684119236817 29.885845669799526 21.653019240853535 41 25.083692977856654 26.688984794095106 25.341830355342232 22.885491872706005 42 26.062757118657743 28.228603694442207 24.082439299830604 21.626199887069454 43 23.3161248689199 29.668468177784945 25.768331047834153 21.247075905461 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 3.5 9 6.0 10 12.5 11 19.0 12 19.0 13 28.0 14 37.0 15 38.0 16 39.0 17 59.0 18 79.0 19 79.0 20 99.0 21 119.0 22 151.0 23 183.0 24 252.5 25 322.0 26 322.0 27 413.5 28 505.0 29 565.0 30 625.0 31 630.5 32 636.0 33 636.0 34 611.0 35 586.0 36 622.5 37 659.0 38 813.5 39 968.0 40 968.0 41 1078.5 42 1189.0 43 1318.5 44 1448.0 45 1843.0 46 2238.0 47 2238.0 48 2487.0 49 2736.0 50 3031.5 51 3327.0 52 3163.0 53 2999.0 54 2999.0 55 2727.0 56 2455.0 57 1898.0 58 1341.0 59 1079.0 60 817.0 61 817.0 62 742.0 63 667.0 64 512.0 65 357.0 66 285.5 67 214.0 68 214.0 69 185.0 70 156.0 71 98.5 72 41.0 73 29.5 74 18.0 75 18.0 76 10.5 77 3.0 78 3.0 79 3.0 80 1.5 81 0.0 82 0.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0040332338468984435 7 0.0 8 0.0040332338468984435 9 0.0040332338468984435 10 0.0 11 0.0040332338468984435 12 0.0040332338468984435 13 0.0 14 0.008066467693796887 15 0.0 16 0.01209970154069533 17 0.0 18 0.0 19 0.02419940308139066 20 0.008066467693796887 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.008066467693796887 38 0.0 39 0.0 40 0.01209970154069533 41 0.0040332338468984435 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 24794.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.48092280390418 #Duplication Level Percentage of deduplicated Percentage of total 1 77.57625111045307 42.264257481648784 2 11.644951140065146 12.688553682342501 3 3.560852827953805 5.819956441074454 4 1.7397098015990524 3.7912398160845364 5 1.221498371335505 3.3274179236912156 6 0.7551080840983121 2.468339114301847 7 0.5626295528575659 2.1456804065499715 8 0.5182114302635475 2.258610954263128 9 0.39976310334616527 1.9601516495926434 >10 1.924785312407462 17.80269420020973 >50 0.06662718389102754 2.282810357344519 >100 0.029612081729345572 3.1902879728966687 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTAGATCGGAGTTCGTCCGACGATCACACTCTAGATCGTTTTT 266 1.0728402032749857 No Hit CTAGATCGACCTCGGTACAGTGCTCTACGCCGGAAGTTCTACC 205 0.8268129386141808 No Hit CTAGATCGAGCACCGACGATCTAGGTGCTGATCGTCGGACTGT 183 0.7380817939824151 No Hit CTAGATCGTTCGCACGACTCACTATAGGGAGTCGTATTACCGG 137 0.5525530370250867 No Hit CTAGATCGCGACTGAACCCCTATAGTGAGTCGTATTACCGGCC 76 0.3065257723642817 No Hit CTAGATCGCCAATGACATCGCTGACGGAGATGTCGTAGTGCTC 74 0.2984593046704848 No Hit CTAGATCGTTATGGAACCCCTATAGTGAGTCGTATTACCGGCC 69 0.27829313543599254 No Hit CTAGATCGCGACTAGTGAGTCGTATTACCGGCCGACGATCTAG 69 0.27829313543599254 No Hit CTAGATCGGAGTGGAACCCCTATAGTGAGTCGTATTACCGGCC 58 0.2339275631201097 No Hit CTAGATCGCGACTGAGTCGTATTACCGGCCGACGATCTAGAGT 58 0.2339275631201097 No Hit CCAGTTCGAGCCGGTCGAAGATCAGGGCCGAGTTGGCGGGAAT 58 0.2339275631201097 No Hit CTAGATCGCGACTACGATCTAGAGTCGGATCGTCGGACTGTAG 53 0.21376139388561746 No Hit CTATATTGAGTCGGCGCGAATGGGCGTGGCTGGCGCTACGCAC 51 0.20569492619182062 No Hit CTAGATCGCTGATGGTCCGACGATCTTTTTCTAGATCGTCGGA 47 0.1895619908042268 No Hit CTAGATCGGAGTGAGTGAGTCGTATTACCGGCCGACGATCTAG 46 0.1855287569573284 No Hit CTAGATCGCGACTGGCCGACGATCTAGAGTCGGATCGTCGGAC 43 0.17342905541663306 No Hit CTAGATCGTTATGACCGGCCGACGATCTAGCATAAGATCGTCG 43 0.17342905541663306 No Hit CTAGATCGTTATGCGGCCGACGATCTAGCATAAGATCGTCGGA 42 0.16939582156973462 No Hit CTAGATCGGAATCGACTCACTATAGGGAGTCGTATTACCGGCC 42 0.16939582156973462 No Hit CTAGATCGCGGGCAAGTCGACCACAATAAAGAGAAAACGAGAT 42 0.16939582156973462 No Hit CTAGATCGGAATCGAGTCGTATTACCGGCCGACGATCTAGGAT 41 0.16536258772283619 No Hit CTAGATCGCTTGTAGTCCGACGATCTTAGGCTAGATCGTTGGA 40 0.16132935387593772 No Hit CTAGATCGCAAGTGGTTCAAATCCCAGCAACCACATGGTGGCT 39 0.15729612002903928 No Hit CTAGATCGCGACTGACGATCTAGAGTCGGATCGTCGGACTGTA 39 0.15729612002903928 No Hit CTAGATGTCAGTTGCAGCCAGGTCACGGTCCTGGCGCGCAGCA 38 0.15326288618214085 No Hit CTAGATCGTTCGCGAACCCCTATAGTGAGTCGTATTACCGGCC 37 0.1492296523352424 No Hit CTAGATCGTTATGGGCCGACGATCTAGCATAAGATCGTCGGAC 37 0.1492296523352424 No Hit CTAGATCGGAGTGGAGTCGTATTACCGGCCGACGATCTAGCAC 37 0.1492296523352424 No Hit CTAGATCGCGACTGCCGACGATCTAGAGTCGGATCGTCGGACT 36 0.14519641848834394 No Hit CTAGATCGTTATGGAGTCGTATTACCGGCCGACGATCTAGCAT 36 0.14519641848834394 No Hit CTAGATCGCGACTAGAACTCTGAACCCCTATAGTGAGTCGTAT 35 0.1411631846414455 No Hit CTAGATCGGAATCGAACCCCTATAGTGAGTCGTATTACCGGCC 34 0.13712995079454707 No Hit CTAGATCGCTCAAGGTTCAGGAAGTTTTTAGCTCTGTACTTGC 33 0.13309671694764863 No Hit CTAGATCGCGACTAGTCGGATCGTCGGACTGTAGAACTCTGAA 32 0.1290634831007502 Illumina DpnII expression Sequencing Primer (100% over 25bp) CTAGATCGGAGTGCCGGCCGACGATCTAGCACTCGATCGTCGG 32 0.1290634831007502 No Hit CCAGTTCGAGCCGGGTCGAAGATCAGGGCCGAGTTGGCGGGAA 32 0.1290634831007502 No Hit CTAGATCGGAGTGACCGGCCGACGATCTAGCACTCGATCGTCG 32 0.1290634831007502 No Hit CTAGATCGAGCACGAACCCCTATAGTGAGTCGTATTACCGGCC 31 0.12503024925385173 No Hit CTAGATCGTTATGGTAGAACTCTGAACCCCTATAGTGAGTCGT 31 0.12503024925385173 No Hit CTAGATCGTTACAAGTCCGACGATCTCAGACTAGATCGTCGGA 31 0.12503024925385173 No Hit CTAGATCGAGGCCGATCCGACGATCGGGTTCTAGAACTCTGAA 30 0.1209970154069533 No Hit CTAGATCGGAGTGACGACTCACTATAGTGAGTCGTATTACCGG 30 0.1209970154069533 No Hit CTAGATCGCGACTAGTCGTATTACCGGCCGACGATCTAGAGTC 30 0.1209970154069533 No Hit CTGGATCGGTTTGGGCTCCACGATGCCGCGGCGCAGCAGCCAC 29 0.11696378156005485 No Hit CTAGATCGGAGTCAGTCCGACGATCGCGCGCTAGATCGTTGGA 29 0.11696378156005485 No Hit CTAGATCGGCTGACAGTCCGACGATCTGGTACTAGATCGTCGG 29 0.11696378156005485 No Hit CTAGATCGGAATCAGTGAGTCGTATTACCGGCCGACGATCTAG 29 0.11696378156005485 No Hit CTAGATCGTTATGATTACCGGCCGACGATCTAGCATAAGATCG 28 0.1129305477131564 No Hit CTAGATCGAACCCGGTTTGAATAAAGAAAGGTATTAATGGGTC 28 0.1129305477131564 No Hit CTAGATCGGAGTGGCCGACGATCTAGCACTCGATCGTCGGACT 26 0.10486408001935951 No Hit CTAGATCGTTATGAGTGAGTCGTATTACCGGCCGACGATCTAG 26 0.10486408001935951 No Hit CTAGATCGTTATGCCGGCCGACGATCTAGCATAAGATCGTCGG 25 0.10083084617246109 No Hit CTAGATCGGAGTGACGATCTAGCACTCGATCGTCGGACTGTAG 25 0.10083084617246109 No Hit CTAGATCGAGCACGAGTCGTATTACCGGCCGACGATCTAGGTG 25 0.10083084617246109 No Hit CTAGATCGGAGTGGGCCGACGATCTAGCACTCGATCGTCGGAC 25 0.10083084617246109 No Hit CTAGATCGCCATGAGTCCGACGATCAAGCTCTAGATCGTCGGA 25 0.10083084617246109 No Hit CTAGATCGTGGGCAGTCCGACGATCGGCATCTAGATCGTCGGA 25 0.10083084617246109 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.01209970154069533 0.0 0.0 0.0 0.0 4 0.01209970154069533 0.0 0.0 0.0 0.0 5 0.01209970154069533 0.0 0.0 0.0 0.0 6 0.01209970154069533 0.0 0.0 0.0 0.0 7 0.01209970154069533 0.0 0.0 0.0 0.0 8 0.01209970154069533 0.0 0.0 0.0 0.0 9 0.01209970154069533 0.0 0.0 0.0 0.0 10 0.01209970154069533 0.0 0.0040332338468984435 0.0 0.0 11 0.01209970154069533 0.0 0.0040332338468984435 0.0 0.0 12 0.01209970154069533 0.0 0.0040332338468984435 0.0 0.0 13 0.01209970154069533 0.0 0.060498507703476645 0.0 0.0 14 0.01209970154069533 0.0 0.29442607082358635 0.0 0.0 15 0.01209970154069533 0.0 1.3390336371702831 0.0 0.0 16 0.01209970154069533 0.0 1.4600306525772364 0.0 0.0 17 0.01209970154069533 0.0 1.637492941840768 0.0 0.0 18 0.01209970154069533 0.0 1.847221101879487 0.0 0.0 19 0.01209970154069533 0.0 2.266677421956925 0.0 0.0 20 0.01209970154069533 0.0 2.657901105106074 0.0 0.0 21 0.01209970154069533 0.0 3.1257562313462937 0.0 0.0 22 0.01209970154069533 0.0 3.3717834960070987 0.0 0.0 23 0.01209970154069533 0.0 3.5734451883520206 0.0 0.0 24 0.01209970154069533 0.0 3.8719044930225053 0.0 0.0 25 0.01209970154069533 0.0 4.331693151568928 0.0 0.0 26 0.01209970154069533 0.0 4.6624183270146 0.0 0.0 27 0.01209970154069533 0.0 5.009276437847866 0.0 0.0 28 0.01209970154069533 0.0 5.654593853351617 0.0 0.0 29 0.01209970154069533 0.0 6.291844801161571 0.0 0.0 30 0.01209970154069533 0.0 7.348552069048964 0.0 0.0 31 0.01209970154069533 0.0 8.49802371541502 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTCGC 80 0.0 37.0 7 CGCGACT 80 0.0 37.0 7 ACAGTGC 20 0.0018073628 37.0 17 TTATGCG 25 1.1990762E-4 37.0 9 CACACTC 40 3.5806806E-8 37.0 25 CGTTATG 100 0.0 37.0 7 CAGTTCG 20 0.0018073628 37.0 2 TCGACCT 20 0.0018073628 37.0 6 GCGACTG 35 5.340571E-7 37.0 8 CCAGTTC 20 0.0018073628 37.0 1 GCGACTA 30 7.988003E-6 37.0 8 TTCGTCC 40 3.5806806E-8 37.0 12 TGGTAGA 20 0.0018073628 37.0 12 GGACCGA 20 0.0018073628 37.0 3 GAAGTTC 20 0.0018073628 37.0 33 CCTCGGT 20 0.0018073628 37.0 10 TCGGCCA 20 0.0018073628 37.0 6 TCGGTCG 20 0.0018073628 37.0 6 GATCGTA 110 0.0 37.0 4 AAATACG 20 0.0018073628 37.0 13 >>END_MODULE