FastQCFastQC Report
Thu 26 May 2016
SRR2099214_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099214_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602132
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGCAATCTGGTCACACTCTTTCAGGGAGAGGGTGTCTT77981.295064869497054No Hit
CTGCTGAATCCAGAAGATATCGTCCTCGACGATGATCAGCTC31100.5164980436183428No Hit
ATGACGCACATGGATTGGTGGCCATGTAGGTCTCCTCTCGCT25650.42598632857911556No Hit
CTGATACAGCTCGGGAGGGGGTGGTAATAATAAATGAAGAAA19150.3180365766974683No Hit
CTGTTGCTCGCGAAGGCACCTCCGTCGGTGACGACTCGACCG16150.26821361429055424No Hit
ATGACGCACATGGGATTGGTGGCCATGTAGGTCTCCTCTCGC14770.24529505158337375No Hit
CTGATGCTGGCACCGGCACAAGCTCCTGTGCGAACACCACGA13270.22038357037991668No Hit
CTGATACAGCTCGGGGAGGGGGTGGTAATAATAAATGAAGAA11910.19779716075544895No Hit
CTGATGCACGCATGCAGACCGGGAGGCCGCATGAGTGGTGCA11000.18268419549201836No Hit
CTGCTGCCCCAAAGTCTTTTTTTTTTTTTTTTTTTTTTTTTT10710.17786797579268332No Hit
CTGATGCATTATGACAGGACCCAGTTTATCAAACATATTAGC10410.1728856795519919No Hit
CTGCTACATGCGGGCGGTGTGTACAAGACCCGGGAACGTATT6580.10927836421249824No Hit
CTGCTGCACAAGCCAGCGCTCCGAACCGTTCTCGGGGGTCAC6360.10562468030265788No Hit
ATGATGCTGTGTTGGAGGTGATGGTCAGCCCCTCGATCCAGC6190.10280137909959944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTA356.9117596E-736.0395289
AGACGGT356.9117596E-736.0395288
CACTCCC356.9117596E-736.0395287
CACCGTA356.9117596E-736.0395287
ACGAGGA356.9117596E-736.0395288
GTGCGTA356.9117596E-736.0395289
TAATGCG356.9117596E-736.0395289
AACGTAC200.00209499836.0395248
ACGTTCG200.00209499836.0395248
CACATCG404.825597E-836.0395247
CACGCGA453.3833203E-936.0395247
CCTACGC251.4392493E-436.0395249
CGTTCGA200.00209499836.0395249
TACATCG1050.036.0395245
CCCGTAC4000.036.0395246
CACTAGT251.4392493E-436.0395247
ATGACGG404.825597E-836.0395248
CTCGCGA2750.036.0395247
TTCGGGG251.4392493E-436.0395249
TCGAGTA404.825597E-836.0395249
Produced by FastQC (version 0.11.5)