FastQCFastQC Report
Thu 26 May 2016
SRR2099213_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099213_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57716
Sequences flagged as poor quality0
Sequence length42
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGCATGGTGGAGCGGACTTGTAAGGACCGCCGCTTTCG3480.602952387552845No Hit
CTGATGCAATGGGCTTCGCACCACACAGCCCTGGGGAATGCA2880.4989950793540786No Hit
CTGATGCACCGGGTTACAGTCTATGAAATAGATAAAGAGACT1920.3326633862360524No Hit
CTGATGCAATCTGGTCACACTCTTTCAGGGAGAGGGTGTCTT1770.3066740591863608No Hit
CTGATGCACACCGTTTCATTCAGCCCAGTAATCAAAAGTGCT1670.2893478411532331No Hit
CTGATGCATCACGTTTTGCCGGCGCACAAGCACGATCGGCTC1430.2477649178737265No Hit
CTGATGCAAGTCGATTCGGCTTCTCGCCATGTCTTCTCACAA1380.23910180885716265No Hit
CTGATGCAAACGATTTTGGCTGACCAGCAGGACAAGTACATG1320.22870607803728601No Hit
CTGATGCATCTCGTGTCTTAGACGACTGTGTGTGATTCTCGA1310.22697345623397322No Hit
CTGATGCACCGGTTACAGTCTATGAAATAGATAAAGAGACTG1070.1853905329544667No Hit
CTGATGCAAATTGGTCCGGATGGTGCTGAATGGCTGAGGACA1020.17672742393790283No Hit
CTGATGTATCCCATTACAGACATGTGTCCCCATGCCTATAAT1010.17499480213459007No Hit
CTGATGCAGCGGAATGCCAGCTGCGTGTCTAGTTTGGAGTGC970.16806431492133897No Hit
CTGATGCATGTCGATCCACACTGATGCATTTTTTTTTTTTTT900.15593596229814954No Hit
CTGATGCAGGAAGATTCAGGTGATGTGGAGCTCAGAAATCCT890.1542033404948368No Hit
CTGATGCATACAGTTGACTAGGCGCATAGTCGCCATTCACAG880.152470718691524No Hit
CTGATGCAAGATGTTGACTAGGCGCATAGTCGCCATTCACAG850.14727285328158568No Hit
CTGATGCAGAGTGTTGACTAGGCGCATAGTCGCCATTCACAG840.1455402314782729No Hit
CTGATGCAGTGGGTTAATAAGGCCTAGCCTAAAGGTTCTTGC830.14380760967496015No Hit
CTGATGCAAGCTGTTTCGCTCGGGTCTGCGGGTTATAGCTTT830.14380760967496015No Hit
CTGATGCAGCTAGCTGCAGCCCATCGGCCGAGTTGACGTCAC820.14207498787164738No Hit
CTGATGCAGACCGTTTCATTCAGCCCAGTAATCAAAAGTGCT810.1403423660683346No Hit
CTGATGCAAACTGTATAGCTTCCCATCTTCTTTGAGAGTTGT800.13860974426502184No Hit
CTGATGCATCAAGTAAAGAAATAGTGGGGATTTTTAAATTTA750.12994663524845798No Hit
CTGATGCACCTCGAGAGGATCTCCTCCGAGATTAGAGCTAAA720.12474876983851965No Hit
CTGATGCAGGCGGTTTATTGGAAGCATCAACCTGCGCCGTCT720.12474876983851965No Hit
CTGATGCAGCGGGTTAATAAGGCCTAGCCTAAAGGTTCTTGC700.1212835262318941No Hit
CTGATGCACTCCCATGCATGTCAGGCTGCCTTCCCCAAAGCC680.11781828262526854No Hit
CTGATGCAAAGTAATTCATGGTGTTCTTTCTCTTCTTCAAGT680.11781828262526854No Hit
CTGATGCAAGATGTAAAAGAAGCGGATTTAATTCTGCATTTA670.11608566082195579No Hit
CTGATGCATTGGAGTGCACGTGCTGTGCCTGATGTGACGAAC670.11608566082195579No Hit
CTGATGCACGGGGTTTCACAGGCCCCTCCTGCCCTGCCCATC660.11435303901864301No Hit
CTGATGCATCAAGTTTCAGTCTCGACGGGCTAGCACACATCT650.11262041721533025No Hit
CTGATGCATGAGGATGCGGGACATGAAAAAACAGTGCTTCGG630.10915517360870469No Hit
CTGATGCATGCCGTTACAGAAAATAAAATTACAGGCTGGGCG630.10915517360870469No Hit
CTGATGCATCTGGTATAGCTTCCCATCTTCTTTGAGAGTTGT620.10742255180539192No Hit
CTGATGCAAATTGAAAAGATCGGGATGCTACTAGTTTACTGC620.10742255180539192No Hit
CTGATGCATTGGGTTTCTAGGGTTAGTGAAGGTTCGATACAT590.10222468639545361No Hit
CTGATGTATCCCATTACAGACATGTGTCCCCATGCCTGGCTA590.10222468639545361No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCATC502.3101165E-1036.00000421
GGGTTAC309.865773E-636.00000411
CACACCG251.4324077E-436.0000047
AACGATT251.4324077E-436.0000049
GCAAGCG309.865773E-636.0000046
GCGACGG251.4324077E-436.00000413
CACGTTA251.4324077E-436.0000047
TGCAAGA502.3101165E-1036.0000045
ATGACGC309.865773E-636.0000041
GCAGGTA251.4324077E-436.0000046
ACCACAC309.865773E-636.00000420
CATCTTC502.3101165E-1036.00000424
ACGCACA309.865773E-636.0000044
CACCGTT251.4324077E-436.0000049
GCAGGGC309.865773E-636.0000046
TAGCTTC502.3101165E-1036.00000416
CACCGAG251.4324077E-436.0000047
ACCGTTT251.4324077E-436.00000410
ATGGCGT309.865773E-636.0000048
CAATTTC251.4324077E-436.00000411
Produced by FastQC (version 0.11.5)