FastQCFastQC Report
Thu 26 May 2016
SRR2099212_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099212_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3388611
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTGCACCACAACCAAAAATAATGAACTTAAAACAAAAATA37940.1119632793495624No Hit
AGTCTGCATGTAGCAGTCCGACGTTCATTGAAGTCTGCATTTT37800.1115501307172762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAA719350.036.5925983
CTGCACG212550.036.5681954
TCTGCAG1019650.036.427623
CTGCATA192750.036.165364
TGCACGA50450.036.151225
CTGCAAC147450.036.1508984
TGCAACG47350.036.1326565
CTGCAAT199050.036.127614
TGCAGCG82050.036.0842555
CTGCAGT264750.035.9808654
TGCACCG48300.035.9586685
TGCACGT55000.035.8537675
GTCTGCA3143150.035.8360862
AGTCTGC3187650.035.8041841
CTGCAAG225100.035.623364
CTGCACA161950.035.5436444
TCTGCAC663400.035.5236323
CTGCATG238700.035.5095834
TGCACGG66750.035.4786575
GCACGTA10550.035.452496
Produced by FastQC (version 0.11.5)