FastQCFastQC Report
Thu 26 May 2016
SRR2099210_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099210_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334164
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCACTAGTTGACGGCGCTGATCGTGCAGCTGGAGCTGCCGAT53260.3992012975916004No Hit
GTCACTAGGTACTATTGAGGCCGTGCATTCTTTCGATCCTGGA40020.299963122974387No Hit
GTCACTAGGAGCTTACAGGAGAAGGGCCGGCAGAGGAGCGTCG25320.18978176595980703No Hit
GTCACTAGCGTGGAGTGGATCCCATGGCTGACACGTGCCCAAG14120.10583406537726997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC1300.037.0364577
GGCAACG355.5889177E-737.0364578
TTGCGAG200.001843995436.99201210
ACTAGCG74450.036.9065484
CACTAGG394450.036.869923
CACTAGC238400.036.858653
CTAGGCG25550.036.7437865
ACTAGAT67850.036.703434
TAGGCCG10250.036.6737486
ACTAGGG119150.036.6734854
CTAGCCG14450.036.6492425
CACTAGA281600.036.628443
ACTAGGT106750.036.597284
ACTAGAC51550.036.42034
ACTAGCA62800.036.4117474
ACTAGGC83400.036.3870744
CTAGCGG21550.036.3462755
ACTAGAG92550.036.3306924
ACTAGGA92850.036.313014
CTAGGGG36100.036.264285
Produced by FastQC (version 0.11.5)