FastQCFastQC Report
Thu 26 May 2016
SRR2099208_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099208_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1901002
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTCAACCGCCGTTGGGGCGGAACACCACTTGCAGCGCCTG53850.28327166410135285No Hit
GCAACCGAAGCGTGCGGCGCGCCGGTGGGCGCGGACCGTCGGG34850.18332437314637226No Hit
GCATTCGAGGTTCAGCGCACCGCGCATGGCCTGGCCGCTGCAG31300.16465001088899434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTCG4550.037.035026
ATTCGAC391500.036.9290163
TTCGACT97300.036.8817984
ATTCGAG469900.036.8518033
TTCGACG115250.036.7769744
TTCGATA99550.036.7736324
TTCGATG128850.036.7322544
TTCGAGC126000.036.666644
TCGACGT32650.036.582265
TCGACGC27800.036.5697175
ATTCGAA357950.036.557163
TTCGAGT134600.036.5525474
TTCGAGG144900.036.5356564
CGAAACG3700.036.5345466
TCGACTC24700.036.5111925
TTCGACC93450.036.4792254
TTCGAAT95750.036.4345364
TTCGAAG103400.036.4072574
TTCGAGA82350.036.4044464
TCGAGCG45100.036.337985
Produced by FastQC (version 0.11.5)