FastQCFastQC Report
Thu 26 May 2016
SRR2099207_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099207_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43167
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTCGATGGGGGTTTCTCATGGCTGACTTGTAGTTCTTCCA1430.331271573192485No Hit
GCGTTCAACCGCCGTTGGGGCGGAACACCACTTGCAGCGCCTG1350.3127388977691292No Hit
GCAACCGAAGCGTGCGGCGCGCCGGTGGGCGCGGACCGTCGGG890.20617601408483333No Hit
GCATTCGACAGACAGTCCGACGATCGGGCTGCATTCGATCGAA830.19227650751731648No Hit
GCATTCGAGGTTCAGCGCACCGCGCATGGCCTGGCCGCTGCAG780.1806935853777191No Hit
GCATTCGAGGCGTGCTTCAGTGAACTGGTTCTTAGTGGGTCTT700.16216090995436327No Hit
GCATTCGAATGTGGTTTTCATCTGAGACCATGAAGCCTGAAAA590.13667848124724905No Hit
CCAATCGACTCACGCGGATTTCGTCGTCTTCCAGCACGTTGAC580.13436189681932956No Hit
GCATTCGAGTTCGGTTTCATTTGCATCTATGTTTTTAGCTATC480.11119605254013483No Hit
GCATTCGAGCGAAGTTTGAATTATGTTCTGTAGGAAAGTGTAG470.10887946811221536No Hit
GCATTCGATGGCTAGTCCGACGATCTGCATGCATTCGTCGGAC450.10424629925637639No Hit
GCATTCGATCGGAAGTCCGACGATCGGATTGCATTCGATTGTC440.10192971482845692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGAA308.077239E-637.03364616
GAGTCCG200.001814237137.0336413
TTCAGTG200.001814237137.0336416
CGACAGA200.001824619536.990736
CGACACT200.001824619536.990736
CGACACC200.001824619536.990736
CGACAAC200.001824619536.990736
CGATATG200.001824619536.990736
CGATAGG251.2158361E-436.990736
CGATAGA251.2158361E-436.990736
TCGACTT900.036.990735
TCGACTG650.036.990735
TCGACTC700.036.990735
TCGACTA251.2158361E-436.990735
TCGATTC551.0913936E-1136.990735
TCGATTA355.476486E-736.990735
CGATAAA403.697278E-836.990736
TCGACGT501.6916601E-1036.990735
TCGACGG1000.036.990735
TCGACGC800.036.990735
Produced by FastQC (version 0.11.5)