FastQCFastQC Report
Thu 26 May 2016
SRR2099206_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099206_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755198
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCATGGTTCTCGATCGTCCTTAGCCATGTTTTTTTTTTTT29210.3867859819544014No Hit
TAGCCCTGGCATTAATCAGACCTCGACGGCACATGGAAGCCCT19770.2617856509153891No Hit
AAGCCATCGTACGCAGGAACAGCGTCAGGCGCGGCACGGGGTT19200.2542379614352792No Hit
TAGCCATGTGTTAAGTTCCAGGGCCTGCTCGGCGGTGGCGCAA16630.2202071509723278No Hit
TAGCCTTGTTCGATGAGCATCCGCCTCACTGAGGTAGAGGCCT16180.21424844875118843No Hit
TAGCCAAGTGTCACGGCTGTCGTGAATGGTGCTTTGGCGATTG15030.19902065418605452No Hit
TCGCCATGGTTCTGCGATCGTCCTTAGCCATGTTTTTTTTTTT13260.1755830921162397No Hit
TAGCCATGGTAGGGGGTACCACTGAGGGTCGTGGCTGGGAGAG12740.1686974806607009No Hit
TAGCCATGACGACCCAGTTCGCGCTCAACACCAGCATCGTGAA11960.15836906347739269No Hit
TAGCCATGAAGAGATCGTGGTTAGCCATGTTTTTTTTTTTTTT11320.14989446476288337No Hit
TAGCCATGCGCCAGTGTAATCCCAGTGCTTTGGGAAGGCAAGG11270.14923238673831232No Hit
TAGCCAATAACATGCGTTCACCGCATCGTCCGGCCGTGGGATC10650.14102261923363146No Hit
TAGCCGCGTATGAGCAGCCACCATCAGCCACATCATGAACGAT10440.1382418915304331No Hit
TAGCCATGTCAGAATTGATTCTTGTTATTCTGTGTGTACATGC9700.12844313676678168No Hit
TAGCCAAGTCGAGGCCTTTGTGACGCCGGACAAACCCATGCGC7870.10421108106748166No Hit
TAGGCATTGTACCAAAGGTTGCTTCTGGTGAGCCAATTAAGAA7610.10076827533971225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG1500.037.062359
ACCTACG200.001826370737.062359
AACGCGC200.001826370737.062359
AGTCGCA251.2210627E-437.062359
ACGCGGA251.2210627E-437.062359
GTCTCGT251.22154E-437.059898
GTCTACG355.5570126E-737.059898
TGTACGG650.037.059897
TGCACGA551.2732926E-1137.059897
TGGGTCG1050.037.0598877
ATGTACG6250.037.054976
ACGCGGT900.037.0033810
TCCGGTA308.306768E-637.00092711
GCCATGG190600.036.712313
GCCATGC136550.036.26563
CCATGGT57300.036.214284
GCCATGA122600.036.191173
CATGGCG13950.036.125275
CCATGGG56750.036.0755424
CCATGTC35850.036.0213554
Produced by FastQC (version 0.11.5)