FastQCFastQC Report
Thu 26 May 2016
SRR2099205_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099205_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23640
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCATGGTTGTGTTTCCCCAGCTTACCCGGTCCCAGGTGAT950.4018612521150592No Hit
TAGCCATGGTGGTAATTCTTCAGCAGAAGAGCTGAGGAGAAGA890.37648054145516074No Hit
TAGCCATGCGCTGGAATCGTGACGGGCAGGAAACCATTACACC870.3680203045685279No Hit
TAGCCATGCCAGGGTTTAGGAAACAGCGGTTTTCCACCATTCT670.28341793570219964No Hit
TCGCCATGGTTCTCGATCGTCCTTAGCCATGTTTTTTTTTTTT640.2707275803722504No Hit
TAGCCATGCGGAAGTTTGGGGATGAATCTTGAATATGGGATAT520.21996615905245345No Hit
TAGCCATGGCGTGGTTTCAGTAGAGTGGCCCCAGGGTGATAGC490.2072758037225042No Hit
AAGCCATCGTACGCAGGAACAGCGTCAGGCGCGGCACGGGGTT490.2072758037225042No Hit
TAGCCATGTGTTAAGTTCCAGGGCCTGCTCGGCGGTGGCGCAA480.20304568527918782No Hit
TAGCCCTGGCATTAATCAGACCTCGACGGCACATGGAAGCCCT420.17766497461928935No Hit
TAGCCATGTGGAAGTTTCAGGGTCTGTAATGAACAAAATAACC410.17343485617597293No Hit
TAGCCATGCCTAGGTATCACTTATCCATTTAAGCACTTGACAG390.1649746192893401No Hit
TAGCCTTGTTCGATGAGCATCCGCCTCACTGAGGTAGAGGCCT380.1607445008460237No Hit
TAGCCATGGATTTAATGCGTGGGCTGACATCTGCTGGCCGAAA380.1607445008460237No Hit
TAGCCAAGTGTCACGGCTGTCGTGAATGGTGCTTTGGCGATTG360.15228426395939085No Hit
TAGCCATGTACGCGATTCACACAATGGCAAGACTGAGGATTTA340.14382402707275804No Hit
TAGCCATGTGCTCATTTCACTAATTCCAATACTTATTATTATT310.1311336717428088No Hit
TAGCCATGCGCCAGTGTAATCCCAGTGCTTTGGGAAGGCAAGG310.1311336717428088No Hit
TAGCCATGAGTCGGTATGGTCTCCCCCGCAGCACTTTCAGTAA300.12690355329949238No Hit
TCGCCATGGTTCTGCGATCGTCCTTAGCCATGTTTTTTTTTTT300.12690355329949238No Hit
TAGCCATGGTAGGGGGTACCACTGAGGGTCGTGGCTGGGAGAG290.12267343485617598No Hit
TAGCCATGAATATGATTCAGCGACTTGTTACTCCTCGTGTCCT290.12267343485617598No Hit
TAGCCATGACGACCCAGTTCGCGCTCAACACCAGCATCGTGAA280.11844331641285956No Hit
TAGCCATGTCCTTATTTATCTGCCACGTGGTGGGCATCACGTA270.11421319796954316No Hit
TAGCCATGGTCAGGATCCGGACTGTAGAACTCTGAACCCCTAT270.11421319796954316Illumina DpnII expression PCR Primer 2 (95% over 22bp)
TAGCCATGGTTTAGTTTCCTACCTGGATGATTTCATGCAGTCT260.10998307952622673No Hit
TAGCCATGCGGAGGATTCAGCTACAGGGTGACCAGCGCAAGAA240.10152284263959391No Hit
TAGCCAAGTCGAGGCCTTTGTGACGCCGGACAAACCCATGCGC240.10152284263959391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTG307.972125E-637.06
TTGTTTA200.001805652537.012
ATGGGGG200.001805652537.06
CATGATT355.326401E-737.05
CATGAGT452.395609E-937.05
CATGAGG307.972125E-637.05
CATGAGC200.001805652537.05
CATGACT200.001805652537.05
CATGACG200.001805652537.05
CATGACC403.5684934E-837.05
CATGACA355.326401E-737.05
TAGGAAT750.037.017
AATTCTT200.001805652537.014
GTATTTC200.001805652537.012
CCATGTG1300.037.04
ATGGTGG200.001805652537.06
AGGAATA750.037.018
CCATGGT1700.037.04
CCATGCC1300.037.04
CCATGAT850.037.04
Produced by FastQC (version 0.11.5)