##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099205_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23640 Sequences flagged as poor quality 0 Sequence length 43 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.54771573604061 37.0 35.0 37.0 33.0 37.0 2 35.31400169204738 35.0 35.0 37.0 33.0 37.0 3 35.50160744500846 37.0 35.0 37.0 33.0 37.0 4 37.90499153976312 39.0 39.0 39.0 35.0 39.0 5 38.05706429780034 39.0 39.0 39.0 37.0 39.0 6 37.19027072758037 39.0 37.0 39.0 35.0 39.0 7 37.44602368866328 39.0 37.0 39.0 35.0 39.0 8 37.563536379018615 39.0 37.0 39.0 35.0 39.0 9 32.142174280879864 33.0 31.0 34.0 31.0 34.0 10 32.36489001692048 34.0 31.0 34.0 31.0 34.0 11 32.39784263959391 34.0 31.0 34.0 31.0 34.0 12 35.86827411167513 37.0 35.0 37.0 35.0 37.0 13 35.8013959390863 37.0 35.0 37.0 35.0 37.0 14 30.92673434856176 31.0 30.0 31.0 30.0 34.0 15 30.409179357021998 31.0 30.0 31.0 26.0 34.0 16 29.339382402707276 31.0 27.0 31.0 25.0 34.0 17 32.19890016920474 35.0 30.0 35.0 25.0 37.0 18 33.967724196277494 35.0 33.0 37.0 30.0 37.0 19 32.31907783417936 35.0 30.0 37.0 25.0 37.0 20 32.03950930626058 35.0 30.0 37.0 25.0 37.0 21 32.95630287648054 35.0 32.0 37.0 27.0 37.0 22 34.90528764805415 37.0 33.0 39.0 28.0 39.0 23 35.414382402707275 37.0 34.0 39.0 30.0 39.0 24 36.053384094754655 37.0 35.0 39.0 32.0 39.0 25 36.45486463620981 37.0 35.0 39.0 32.0 39.0 26 36.47901861252115 37.0 35.0 39.0 32.0 39.0 27 37.88164128595601 39.0 37.0 40.0 33.0 41.0 28 38.066285956006766 39.0 37.0 40.0 34.0 41.0 29 38.16370558375635 40.0 37.0 40.0 34.0 41.0 30 38.21789340101523 40.0 37.0 40.0 35.0 41.0 31 38.191793570219964 40.0 37.0 40.0 34.0 41.0 32 38.31992385786802 40.0 37.0 40.0 35.0 41.0 33 38.38561759729272 40.0 37.0 40.0 35.0 41.0 34 38.43891708967851 40.0 38.0 40.0 35.0 41.0 35 38.43900169204738 40.0 38.0 40.0 35.0 41.0 36 38.4914551607445 40.0 38.0 40.0 35.0 41.0 37 38.472546531302875 40.0 38.0 40.0 35.0 41.0 38 38.40478003384095 40.0 38.0 40.0 35.0 41.0 39 38.35744500846024 40.0 38.0 40.0 35.0 41.0 40 38.31099830795262 40.0 37.0 40.0 35.0 41.0 41 38.20896785109983 40.0 37.0 40.0 34.0 41.0 42 38.19881556683587 40.0 37.0 40.0 34.0 41.0 43 38.090313028764804 40.0 37.0 40.0 34.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.03583002333 1101 2 0.8909557531713332 1101 3 0.8539430814643723 1101 4 0.3874931244466424 1101 5 0.12285724261415254 1101 6 0.6289323340847801 1101 7 0.656057288070599 1101 8 -0.11401025115780072 1101 9 0.7430594677003697 1101 10 0.823539067939187 1101 11 0.6689847289226734 1101 12 0.07971630203754643 1101 13 0.8751399892430456 1101 14 -0.26591083892711254 1101 15 -1.410165687921154 1101 16 -0.3990093590179704 1101 17 -1.38230090032663 1101 18 -0.0908588547554956 1101 19 -1.1264505505085367 1101 20 -1.8941742286903178 1101 21 -1.5555455085011118 1101 22 -1.4117670664209712 1101 23 -0.22432134520599334 1101 24 -0.2476532889929146 1101 25 -0.29274900901227596 1101 26 -0.41239832387957875 1101 27 -0.32525254370463585 1101 28 0.34906824223190824 1101 29 0.015166234496525988 1101 30 -0.794854206483592 1101 31 -0.0779457510393442 1101 32 0.07745943327295635 1101 33 -0.0793627455677921 1101 34 0.2645542766151223 1101 35 -0.3131003734926594 1101 36 -0.19623446364975194 1101 37 -0.49096929111843224 1101 38 0.5420373461398498 1101 39 0.4735381249021273 1101 40 0.5466719859369746 1101 41 0.5368988570166238 1101 42 -0.11106006795553469 1101 43 -0.33303195259691876 1102 1 0.6450254256288517 1102 2 0.31279483363110216 1102 3 0.765437334337939 1102 4 0.7438149635271003 1102 5 0.7504434495107049 1102 6 0.933530035234206 1102 7 0.867551540944163 1102 8 0.8020817028651877 1102 9 0.5660479734474961 1102 10 0.5534241254104515 1102 11 0.6023180622560034 1102 12 0.32109561238237205 1102 13 0.6567491846453493 1102 14 0.1375374369349558 1102 15 0.3817883350673519 1102 16 0.319381445579733 1102 17 -0.6340250382576649 1102 18 -0.6494795444106671 1102 19 -1.7781746884395737 1102 20 -0.3723351482305475 1102 21 -0.7141661981562848 1102 22 -0.22441074458188837 1102 23 -0.35880410382668515 1102 24 -0.15340041542969374 1102 25 -0.5640133768283704 1102 26 -0.9296397031899204 1102 27 -0.3057123138195834 1102 28 -0.4647248612163679 1102 29 -1.3618452597563504 1102 30 -0.19370478119623158 1102 31 0.02780137539743066 1102 32 -0.7041497621293473 1102 33 -0.5839604467172208 1102 34 -0.7147560682124663 1102 35 0.0029915805303346588 1102 36 0.12445519152266371 1102 37 0.09523760543329018 1102 38 0.6110028633812306 1102 39 0.675836975476841 1102 40 0.4064421008795094 1102 41 -0.16195171769602013 1102 42 0.8234226906651543 1102 43 0.656623219816872 1103 1 0.7221045331339226 1103 2 0.775269478661528 1103 3 0.6853156304839843 1103 4 0.6423950852309517 1103 5 0.3934454779082657 1103 6 0.7955990007514444 1103 7 0.7874298370902082 1103 8 0.4663819057049423 1103 9 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1104 2 -0.6327001608071612 1104 3 -0.6761644454173421 1104 4 -1.0748724669512129 1104 5 -1.2061750154503699 1104 6 0.15043771042886078 1104 7 -0.31598572268208613 1104 8 -1.035515627501887 1104 9 -0.8655426828372654 1104 10 -1.2786114696952176 1104 11 -1.3009077441956087 1104 12 -0.27512240763986995 1104 13 -0.7915266774236187 1104 14 0.5824762578470803 1104 15 0.978006355089601 1104 16 0.8966895657132135 1104 17 0.14350555128627462 1104 18 0.10914114524450724 1104 19 2.063872030136622 1104 20 2.649911792815061 1104 21 1.1089706205311458 1104 22 1.2516737937940832 1104 23 0.8047109128585248 1104 24 -0.11001888039076846 1104 25 -0.5873726649262565 1104 26 0.7488919987010689 1104 27 1.075822725112566 1104 28 0.2716488873931979 1104 29 0.7872092452492083 1104 30 0.062135040828238175 1104 31 0.4360327435843061 1104 32 0.4538035192944605 1104 33 0.4571963942171564 1104 34 -0.012865078223590842 1104 35 0.1331361856471247 1104 36 -0.20591188300458896 1104 37 0.12193393468801617 1104 38 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17 2.1339153158895883 2216 18 1.7307627668661283 2216 19 2.827603503545518 2216 20 2.864384329868244 2216 21 0.828238275282672 2216 22 1.9116563570024567 2216 23 0.8648678439831912 2216 24 1.4244187830791546 2216 25 0.8712149549516894 2216 26 -0.16915508063633666 2216 27 0.08105376260166963 2216 28 0.7869060800697483 2216 29 0.9755265948568805 2216 30 -0.12007943170881674 2216 31 0.44998217688858233 2216 32 0.41980177561529786 2216 33 0.013430047225000408 2216 34 -0.09220248014163701 2216 35 -0.3176048779971623 2216 36 -0.14488311229839468 2216 37 0.48200368185454323 2216 38 0.21771302181552699 2216 39 0.3095741609381619 2216 40 0.04577108503607974 2216 41 0.20897092908869297 2216 42 0.5186696617741902 2216 43 -0.16816708773205846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 4.0 27 24.0 28 63.0 29 127.0 30 263.0 31 426.0 32 720.0 33 1310.0 34 2587.0 35 4594.0 36 6279.0 37 4272.0 38 2971.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.6641285956006768 1.4086294416243654 97.09390862944163 0.8333333333333334 2 0.892554991539763 97.76226734348562 0.35109983079526225 0.994077834179357 3 97.45769881556684 0.9813874788494078 0.5160744500846024 1.044839255499154 4 0.4145516074450084 0.3299492385786802 0.5372250423011845 98.71827411167513 5 0.3849407783417936 0.7106598984771574 0.3680203045685279 98.53637901861252 6 0.5922416345869115 96.80189517323068 1.036422860527095 1.5694403316553154 7 1.3663282571912012 3.1175972927241964 94.54314720812182 0.972927241962775 8 95.86699945006134 1.476373789077372 1.7471128220313887 0.9095139388298997 9 29.957698815566836 20.46954314720812 27.072758037225043 22.5 10 28.802876480541457 21.692047377326563 26.480541455160743 23.024534686971236 11 26.041710732264477 22.784381742036466 28.262616861965395 22.91129066373366 12 27.183891027539236 21.66335293371124 28.626422437497357 22.52633360125217 13 24.81387478849408 22.495769881556686 29.289340101522843 23.4010152284264 14 55.4253564025551 25.305638986420746 8.57058251195059 10.698422099073564 15 24.53045685279188 26.84010152284264 36.56091370558376 12.068527918781726 16 13.168358714043993 19.82233502538071 55.44839255499154 11.560913705583756 17 13.155668358714042 18.299492385786802 57.64805414551607 10.89678510998308 18 15.047167815897458 29.244045856423707 12.970091797453362 42.73869453022547 19 44.20090551347692 24.8508441585918 14.805568484745907 16.14268184318538 20 27.173499175022208 26.801201506113298 23.594364767102423 22.430934551762068 21 19.7419627749577 29.187817258883246 24.949238578680202 26.12098138747885 22 23.13451776649746 31.125211505922167 24.052453468697124 21.68781725888325 23 22.884940778341793 25.807952622673437 31.120981387478853 20.186125211505924 24 22.944162436548226 28.096446700507617 27.30541455160744 21.653976311336717 25 19.995769881556683 24.534686971235196 33.15989847715736 22.309644670050762 26 21.6243654822335 27.45346869712352 29.59813874788494 21.324027072758035 27 25.2834179357022 27.06429780033841 26.32402707275804 21.328257191201352 28 22.17005076142132 29.805414551607445 27.753807106598984 20.27072758037225 29 20.854483925549914 29.59390862944162 28.752115059221655 20.799492385786802 30 21.476311336717426 28.87055837563452 29.885786802030456 19.767343485617598 31 21.56937394247039 30.765651438240273 28.9424703891709 18.722504230118446 32 20.702199661590527 30.702199661590523 28.320642978003384 20.274957698815566 33 20.943316412859563 30.871404399323183 29.272419627749578 18.912859560067684 34 20.72335025380711 28.97631133671743 29.39086294416244 20.90947546531303 35 20.148054145516074 29.957698815566836 29.25549915397631 20.638747884940777 36 19.631979695431472 30.98138747884941 29.509306260575297 19.877326565143825 37 20.813063158340032 31.211134142730234 28.749101061804645 19.22670163712509 38 20.896785109983078 30.2326565143824 30.10575296108291 18.764805414551606 39 22.06006768189509 30.98138747884941 28.43485617597293 18.523688663282574 40 20.51954645456084 31.15163310204772 29.713149433068203 18.615671010323236 41 19.88155668358714 32.63536379018612 28.87055837563452 18.61252115059222 42 19.521996615905245 32.11082910321489 29.027072758037225 19.34010152284264 43 18.35871404399323 31.302876480541453 30.592216582064296 19.746192893401016 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 2.5 9 4.0 10 8.5 11 13.0 12 13.0 13 25.5 14 38.0 15 59.0 16 80.0 17 128.0 18 176.0 19 176.0 20 255.0 21 334.0 22 443.0 23 552.0 24 659.5 25 767.0 26 767.0 27 796.0 28 825.0 29 758.5 30 692.0 31 727.0 32 762.0 33 762.0 34 841.0 35 920.0 36 1012.0 37 1104.0 38 1279.5 39 1455.0 40 1455.0 41 1640.0 42 1825.0 43 1865.0 44 1905.0 45 1924.0 46 1943.0 47 1943.0 48 1951.5 49 1960.0 50 1903.0 51 1846.0 52 1845.5 53 1845.0 54 1845.0 55 1758.5 56 1672.0 57 1292.0 58 912.0 59 850.5 60 789.0 61 789.0 62 624.5 63 460.0 64 423.5 65 387.0 66 277.0 67 167.0 68 167.0 69 125.0 70 83.0 71 73.0 72 63.0 73 44.5 74 26.0 75 26.0 76 17.0 77 8.0 78 15.5 79 23.0 80 12.5 81 2.0 82 2.0 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.004230118443316413 7 0.0 8 0.004230118443316413 9 0.0 10 0.0 11 0.004230118443316413 12 0.004230118443316413 13 0.0 14 0.004230118443316413 15 0.0 16 0.0 17 0.0 18 0.004230118443316413 19 0.02961082910321489 20 0.012690355329949238 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.004230118443316413 38 0.0 39 0.0 40 0.01692047377326565 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 23640.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.54060913705584 #Duplication Level Percentage of deduplicated Percentage of total 1 78.35696691298848 49.78849407783418 2 11.810132481192996 15.008460236886634 3 4.194128220491312 7.994923857868021 4 2.003861260901405 5.093062605752961 5 1.1117768457492845 3.532148900169205 6 0.6723919845549564 2.563451776649746 7 0.4726715931029891 2.1023688663282574 8 0.32620997270487984 1.6582064297800336 9 0.16643365954330605 0.951776649746193 >10 0.8454829904799948 9.38663282571912 >50 0.039944078290393446 1.9204737732656514 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TAGCCATGGTTGTGTTTCCCCAGCTTACCCGGTCCCAGGTGAT 95 0.4018612521150592 No Hit TAGCCATGGTGGTAATTCTTCAGCAGAAGAGCTGAGGAGAAGA 89 0.37648054145516074 No Hit TAGCCATGCGCTGGAATCGTGACGGGCAGGAAACCATTACACC 87 0.3680203045685279 No Hit TAGCCATGCCAGGGTTTAGGAAACAGCGGTTTTCCACCATTCT 67 0.28341793570219964 No Hit TCGCCATGGTTCTCGATCGTCCTTAGCCATGTTTTTTTTTTTT 64 0.2707275803722504 No Hit TAGCCATGCGGAAGTTTGGGGATGAATCTTGAATATGGGATAT 52 0.21996615905245345 No Hit TAGCCATGGCGTGGTTTCAGTAGAGTGGCCCCAGGGTGATAGC 49 0.2072758037225042 No Hit AAGCCATCGTACGCAGGAACAGCGTCAGGCGCGGCACGGGGTT 49 0.2072758037225042 No Hit TAGCCATGTGTTAAGTTCCAGGGCCTGCTCGGCGGTGGCGCAA 48 0.20304568527918782 No Hit TAGCCCTGGCATTAATCAGACCTCGACGGCACATGGAAGCCCT 42 0.17766497461928935 No Hit TAGCCATGTGGAAGTTTCAGGGTCTGTAATGAACAAAATAACC 41 0.17343485617597293 No Hit TAGCCATGCCTAGGTATCACTTATCCATTTAAGCACTTGACAG 39 0.1649746192893401 No Hit TAGCCTTGTTCGATGAGCATCCGCCTCACTGAGGTAGAGGCCT 38 0.1607445008460237 No Hit TAGCCATGGATTTAATGCGTGGGCTGACATCTGCTGGCCGAAA 38 0.1607445008460237 No Hit TAGCCAAGTGTCACGGCTGTCGTGAATGGTGCTTTGGCGATTG 36 0.15228426395939085 No Hit TAGCCATGTACGCGATTCACACAATGGCAAGACTGAGGATTTA 34 0.14382402707275804 No Hit TAGCCATGTGCTCATTTCACTAATTCCAATACTTATTATTATT 31 0.1311336717428088 No Hit TAGCCATGCGCCAGTGTAATCCCAGTGCTTTGGGAAGGCAAGG 31 0.1311336717428088 No Hit TAGCCATGAGTCGGTATGGTCTCCCCCGCAGCACTTTCAGTAA 30 0.12690355329949238 No Hit TCGCCATGGTTCTGCGATCGTCCTTAGCCATGTTTTTTTTTTT 30 0.12690355329949238 No Hit TAGCCATGGTAGGGGGTACCACTGAGGGTCGTGGCTGGGAGAG 29 0.12267343485617598 No Hit TAGCCATGAATATGATTCAGCGACTTGTTACTCCTCGTGTCCT 29 0.12267343485617598 No Hit TAGCCATGACGACCCAGTTCGCGCTCAACACCAGCATCGTGAA 28 0.11844331641285956 No Hit TAGCCATGTCCTTATTTATCTGCCACGTGGTGGGCATCACGTA 27 0.11421319796954316 No Hit TAGCCATGGTCAGGATCCGGACTGTAGAACTCTGAACCCCTAT 27 0.11421319796954316 Illumina DpnII expression PCR Primer 2 (95% over 22bp) TAGCCATGGTTTAGTTTCCTACCTGGATGATTTCATGCAGTCT 26 0.10998307952622673 No Hit TAGCCATGCGGAGGATTCAGCTACAGGGTGACCAGCGCAAGAA 24 0.10152284263959391 No Hit TAGCCAAGTCGAGGCCTTTGTGACGCCGGACAAACCCATGCGC 24 0.10152284263959391 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.004230118443316413 0.0 0.0 15 0.0 0.0 0.03807106598984772 0.0 0.0 16 0.0 0.0 0.03807106598984772 0.0 0.0 17 0.0 0.0 0.03807106598984772 0.0 0.0 18 0.0 0.0 0.03807106598984772 0.0 0.0 19 0.0 0.0 0.04230118443316413 0.0 0.0 20 0.0 0.0 0.04230118443316413 0.0 0.0 21 0.0 0.0 0.04230118443316413 0.0 0.0 22 0.0 0.0 0.08037225042301184 0.0 0.0 23 0.0 0.0 0.12267343485617598 0.0 0.0 24 0.0 0.0 0.2580372250423012 0.0 0.0 25 0.0 0.0 0.26649746192893403 0.0 0.0 26 0.0 0.0 0.37225042301184436 0.0 0.0 27 0.0 0.0 0.3934010152284264 0.0 0.0 28 0.0 0.0 0.41455160744500846 0.0 0.0 29 0.0 0.0 0.44416243654822335 0.0 0.0 30 0.0 0.0 0.4780033840947547 0.0 0.0 31 0.0 0.0 0.5076142131979695 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGTG 30 7.972125E-6 37.0 6 TTGTTTA 20 0.0018056525 37.0 12 ATGGGGG 20 0.0018056525 37.0 6 CATGATT 35 5.326401E-7 37.0 5 CATGAGT 45 2.395609E-9 37.0 5 CATGAGG 30 7.972125E-6 37.0 5 CATGAGC 20 0.0018056525 37.0 5 CATGACT 20 0.0018056525 37.0 5 CATGACG 20 0.0018056525 37.0 5 CATGACC 40 3.5684934E-8 37.0 5 CATGACA 35 5.326401E-7 37.0 5 TAGGAAT 75 0.0 37.0 17 AATTCTT 20 0.0018056525 37.0 14 GTATTTC 20 0.0018056525 37.0 12 CCATGTG 130 0.0 37.0 4 ATGGTGG 20 0.0018056525 37.0 6 AGGAATA 75 0.0 37.0 18 CCATGGT 170 0.0 37.0 4 CCATGCC 130 0.0 37.0 4 CCATGAT 85 0.0 37.0 4 >>END_MODULE